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The map label for this gene is acnB [H]

Identifier: 161897780

GI number: 161897780

Start: 2324364

End: 2326967

Strand: Direct

Name: acnB [H]

Synonym: Csal_2058

Alternate gene names: 161897780

Gene position: 2324364-2326967 (Clockwise)

Preceding gene: 92114176

Following gene: 92114181

Centisome position: 62.88

GC content: 66.47

Gene sequence:

>2604_bases
GTGCTTGAAGCCTATCGCCAACATGTCGAGGAGCGCGCCCAGGCGGGCGTTCCGCCAAAGCCGCTGAACGCCGAGCAGAC
GGCTGCCCTGGTCAAGCTGCTCAAGAATCCGCCCGCCGGCGAGGAAGAGGCCATTCTCGACCTGCTGACCAACCGCGTGC
CGCCGGGCGTCGACGAGGCCGCCTACGTCAAGGCCGGCTTCCTGACCGCCATCGCCAAGGGCGAGGCCGAGTCCCCGCTG
ATCGACCGCGTCCACGCCGTCAAGCTGCTGGGCACCATGCAGGGCGGTTACAACATCGTCTCGCTGGTCGAGCTGCTGGA
TGACGACGACCTGGCCCGCGAAGTCGGCGAGCAGCTCAAGCACACCCTGCTGATGTTCGATGCCTTCCATGACGTGGAAG
AGCGTGCCAAGGCCGGTAACGCCGTGGCCAAGGAGGTCATCGCGTCCTGGGCCGAGGCCGAGTGGTTCCTCGCAAAGCCC
AAGCTGGCCGACAAGATCACCCTGACCGTGTTCAAGGTGCCCGGCGAGACCAACACCGATGACCTCTCCCCGGCCCCGGA
TGCCTGGTCGCGCCCGGACATCCCGCTGCATGCCAATGCCATGCTCAAGAACCCGCGCGACGGCATCGAGCCGGTCGAAC
CGGGCGTCACCGGTCCGCTCAAGCAGATCGAGGAAGTGAAAGCCAAGGGCCACCCGGTCGTCTACGTCGGCGACGTGGTC
GGCACCGGCTCCTCGCGCAAGTCCGCCACCAACTCCGTGCTGTGGTTCTTCGGCGACGACATCCCCAATGTGCCGAACAA
GCGCGCCGGCGGCTTCTGCTTCGGCGGCAAGATCGCCCCGATCTTCTTCAATACCATGGAAGACTCCGGCGCCCTGCCCA
TCGAGATGGACGTCGAGAAGCTCGAGATGGGCGACGTCATCGACGTCTACCCGTATGAAGGCAAGGTCTGCAAGCACGGC
ACCGACGAGGTGCTGACCACCTTCGAGCTCAAGACCCAGCTGCTGCTCGACGAAGCCCGTGCCGGCGGCCGCATCCCGCT
GATCATCGGCCGCGGCCTGACCGCCAAGGCCCGCGAGTCCCTGGGCCTGGCCCCATCCGACGTCTTCCGCCTGCCGGAGC
AGCCGGCCGATACCGGTCGCGGCTTCACCCTGGCCCAGAAGATGGTCGGCAAGGCCTGCGGCATGGACGGCGTGCGTCCC
GGCATGTACTGCGAGCCGAAGATGACCACCGTGGGTTCCCAGGACACCACCGGTCCGATGACCCGCGACGAGCTCAAGGA
CCTGGCCTGCCTGGGCTTCCAGGCCGACCTGGTGATGCAGTCCTTCTGCCACACCAGCGCCTATCCCAAGCCGGTCGACG
TGGAGACCCACCACACCCTGCCCGACTTCATCATGAATCGCGGCGGCGTCTCGCTGCGTCCGGGCGACGGCATCATCCAC
AGCTGGCTCAACCGCATGCTGCTGCCCGACACCGTGGGTACCGGCGGCGACTCCCACACCCGTTTCCCGATGGGCATCTC
CTTCCCGGCGGGCTCCGGCCTGGTGGCCTTCGCCGCCGCCACCGGCGTGATGCCGCTGGACATGCCGGAATCGGTGCTGG
TGCGCTTCAAGGGCAAGCGTCAGCCCGGCGTCACCCTGCGTGACCTGGTCCACGCCATCCCCTACTACGCCATCCAGCAG
GGCCTGCTGACCGTCGAGAAGTCCGGCAAGAAGAACGCCTTCTCCGGCCGCGTGCTGGAGATCGAGGGCCTCGAGGACCT
CACCGTCGAGCAGGCCTTCGAACTCTCCGACGCCTCCGCCGAGCGCAGCGCCGCCGGCTGCACCATCACCCTCTCCGAGG
ACAGTGTCAGCGAGTACCTGAAGTCCAACATCACCTTGCTCAAGTGGATGATCGCCAACGGCTACGGCGACGCGCGCACC
ATCGAGCGCCGCATCAAGGGCATGGAAGAATGGCTCGAGAACCCGAGCCTGATGCGCGCCGACAAGGACGCCGAGTACGC
CGAGGTCATCGAGATCGATCTCGACCAGCTCAGCGAGCCGGTGCTGTGCGCCCCGAACGACCCGGACGACGCCCGCCTGC
TCTCCGAGGTGGCCGGCCAGAGCATCGACGAGGTGTTCATCGGCTCGTGCATGACCAACATCGGCCACTTCCGTGCCGCC
GGCAAGCTGCTCGAGAAGCAGCCCGCGGGCAGCCTGAAGACCAAGCTGTGGCTGGCGCCGCCGACCAAGATGGACCAGCA
CCAGCTCACCGAGGAAGGCTACTACGGCATCTACGGCCGTGCCGGTGCGCGCATGGAAATGCCCGGCTGCTCGCTGTGCA
TGGGTAACCAGGCCCGCGTCGCCGCCAAGAGCACCGTGGTGTCCACCTCCACGCGCAACTTCCCCAACCGTCTGGGCGAT
GGCGCCGATGTCTTCCTGGCCTCCGCCGAGCTTGCCGCCGTGGCCGCGGTGGAAGGACGCCTGCCGAGCGTCGACGAATA
TCGCCGTTACATGAGCGAATTCGACGCCATGGCCGGCGAGATCTATCGTTACATGAACTTCCATGAAATCGAAGAGTTCC
AGAACGCGGCCTCGAACGTGATTCCGGTCGCCGAGGAAGCCTGA

Upstream 100 bases:

>100_bases
GGTGAAACCTGCACCAGGTCGGTGGGGAGGCGGTGACGGCCGAGCGCCTGCCCATGACTCGCCGCCGCCCTGGTTTCGAT
GACTGATGAGGGTCCCCAAC

Downstream 100 bases:

>100_bases
CCACTCGTCTCCAATGCTCGCCTGTTGAGAAAGAGTGCAGGTCACCGAAACGCCCCGCCGATGCGGGGCGTTTTTTTGAG
CCTGTCGCCATCCGGCAACG

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 867; Mature: 867

Protein sequence:

>867_residues
MLEAYRQHVEERAQAGVPPKPLNAEQTAALVKLLKNPPAGEEEAILDLLTNRVPPGVDEAAYVKAGFLTAIAKGEAESPL
IDRVHAVKLLGTMQGGYNIVSLVELLDDDDLAREVGEQLKHTLLMFDAFHDVEERAKAGNAVAKEVIASWAEAEWFLAKP
KLADKITLTVFKVPGETNTDDLSPAPDAWSRPDIPLHANAMLKNPRDGIEPVEPGVTGPLKQIEEVKAKGHPVVYVGDVV
GTGSSRKSATNSVLWFFGDDIPNVPNKRAGGFCFGGKIAPIFFNTMEDSGALPIEMDVEKLEMGDVIDVYPYEGKVCKHG
TDEVLTTFELKTQLLLDEARAGGRIPLIIGRGLTAKARESLGLAPSDVFRLPEQPADTGRGFTLAQKMVGKACGMDGVRP
GMYCEPKMTTVGSQDTTGPMTRDELKDLACLGFQADLVMQSFCHTSAYPKPVDVETHHTLPDFIMNRGGVSLRPGDGIIH
SWLNRMLLPDTVGTGGDSHTRFPMGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKRQPGVTLRDLVHAIPYYAIQQ
GLLTVEKSGKKNAFSGRVLEIEGLEDLTVEQAFELSDASAERSAAGCTITLSEDSVSEYLKSNITLLKWMIANGYGDART
IERRIKGMEEWLENPSLMRADKDAEYAEVIEIDLDQLSEPVLCAPNDPDDARLLSEVAGQSIDEVFIGSCMTNIGHFRAA
GKLLEKQPAGSLKTKLWLAPPTKMDQHQLTEEGYYGIYGRAGARMEMPGCSLCMGNQARVAAKSTVVSTSTRNFPNRLGD
GADVFLASAELAAVAAVEGRLPSVDEYRRYMSEFDAMAGEIYRYMNFHEIEEFQNAASNVIPVAEEA

Sequences:

>Translated_867_residues
MLEAYRQHVEERAQAGVPPKPLNAEQTAALVKLLKNPPAGEEEAILDLLTNRVPPGVDEAAYVKAGFLTAIAKGEAESPL
IDRVHAVKLLGTMQGGYNIVSLVELLDDDDLAREVGEQLKHTLLMFDAFHDVEERAKAGNAVAKEVIASWAEAEWFLAKP
KLADKITLTVFKVPGETNTDDLSPAPDAWSRPDIPLHANAMLKNPRDGIEPVEPGVTGPLKQIEEVKAKGHPVVYVGDVV
GTGSSRKSATNSVLWFFGDDIPNVPNKRAGGFCFGGKIAPIFFNTMEDSGALPIEMDVEKLEMGDVIDVYPYEGKVCKHG
TDEVLTTFELKTQLLLDEARAGGRIPLIIGRGLTAKARESLGLAPSDVFRLPEQPADTGRGFTLAQKMVGKACGMDGVRP
GMYCEPKMTTVGSQDTTGPMTRDELKDLACLGFQADLVMQSFCHTSAYPKPVDVETHHTLPDFIMNRGGVSLRPGDGIIH
SWLNRMLLPDTVGTGGDSHTRFPMGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKRQPGVTLRDLVHAIPYYAIQQ
GLLTVEKSGKKNAFSGRVLEIEGLEDLTVEQAFELSDASAERSAAGCTITLSEDSVSEYLKSNITLLKWMIANGYGDART
IERRIKGMEEWLENPSLMRADKDAEYAEVIEIDLDQLSEPVLCAPNDPDDARLLSEVAGQSIDEVFIGSCMTNIGHFRAA
GKLLEKQPAGSLKTKLWLAPPTKMDQHQLTEEGYYGIYGRAGARMEMPGCSLCMGNQARVAAKSTVVSTSTRNFPNRLGD
GADVFLASAELAAVAAVEGRLPSVDEYRRYMSEFDAMAGEIYRYMNFHEIEEFQNAASNVIPVAEEA
>Mature_867_residues
MLEAYRQHVEERAQAGVPPKPLNAEQTAALVKLLKNPPAGEEEAILDLLTNRVPPGVDEAAYVKAGFLTAIAKGEAESPL
IDRVHAVKLLGTMQGGYNIVSLVELLDDDDLAREVGEQLKHTLLMFDAFHDVEERAKAGNAVAKEVIASWAEAEWFLAKP
KLADKITLTVFKVPGETNTDDLSPAPDAWSRPDIPLHANAMLKNPRDGIEPVEPGVTGPLKQIEEVKAKGHPVVYVGDVV
GTGSSRKSATNSVLWFFGDDIPNVPNKRAGGFCFGGKIAPIFFNTMEDSGALPIEMDVEKLEMGDVIDVYPYEGKVCKHG
TDEVLTTFELKTQLLLDEARAGGRIPLIIGRGLTAKARESLGLAPSDVFRLPEQPADTGRGFTLAQKMVGKACGMDGVRP
GMYCEPKMTTVGSQDTTGPMTRDELKDLACLGFQADLVMQSFCHTSAYPKPVDVETHHTLPDFIMNRGGVSLRPGDGIIH
SWLNRMLLPDTVGTGGDSHTRFPMGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKRQPGVTLRDLVHAIPYYAIQQ
GLLTVEKSGKKNAFSGRVLEIEGLEDLTVEQAFELSDASAERSAAGCTITLSEDSVSEYLKSNITLLKWMIANGYGDART
IERRIKGMEEWLENPSLMRADKDAEYAEVIEIDLDQLSEPVLCAPNDPDDARLLSEVAGQSIDEVFIGSCMTNIGHFRAA
GKLLEKQPAGSLKTKLWLAPPTKMDQHQLTEEGYYGIYGRAGARMEMPGCSLCMGNQARVAAKSTVVSTSTRNFPNRLGD
GADVFLASAELAAVAAVEGRLPSVDEYRRYMSEFDAMAGEIYRYMNFHEIEEFQNAASNVIPVAEEA

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=861, Percent_Identity=76.0743321718931, Blast_Score=1334, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=398, Percent_Identity=25.3768844221106, Blast_Score=76, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6323335, Length=384, Percent_Identity=26.5625, Blast_Score=78, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6322261, Length=399, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 94103; Mature: 94103

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00450 ACONITASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEAYRQHVEERAQAGVPPKPLNAEQTAALVKLLKNPPAGEEEAILDLLTNRVPPGVDEA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCH
AYVKAGFLTAIAKGEAESPLIDRVHAVKLLGTMQGGYNIVSLVELLDDDDLAREVGEQLK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHH
HTLLMFDAFHDVEERAKAGNAVAKEVIASWAEAEWFLAKPKLADKITLTVFKVPGETNTD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEEECCCCCCCC
DLSPAPDAWSRPDIPLHANAMLKNPRDGIEPVEPGVTGPLKQIEEVKAKGHPVVYVGDVV
CCCCCCCCCCCCCCCEECHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCEE
GTGSSRKSATNSVLWFFGDDIPNVPNKRAGGFCFGGKIAPIFFNTMEDSGALPIEMDVEK
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEEEEEEEEECCCCCCCCEEECHHH
LEMGDVIDVYPYEGKVCKHGTDEVLTTFELKTQLLLDEARAGGRIPLIIGRGLTAKARES
HCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
LGLAPSDVFRLPEQPADTGRGFTLAQKMVGKACGMDGVRPGMYCEPKMTTVGSQDTTGPM
CCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCC
TRDELKDLACLGFQADLVMQSFCHTSAYPKPVDVETHHTLPDFIMNRGGVSLRPGDGIIH
CHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCEECCCCCHHH
SWLNRMLLPDTVGTGGDSHTRFPMGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKR
HHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCEEHHHHCCCCCCCCCHHHHHEECCCC
QPGVTLRDLVHAIPYYAIQQGLLTVEKSGKKNAFSGRVLEIEGLEDLTVEQAFELSDASA
CCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCC
ERSAAGCTITLSEDSVSEYLKSNITLLKWMIANGYGDARTIERRIKGMEEWLENPSLMRA
HHCCCCEEEEECCHHHHHHHHCCCCEEEHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEC
DKDAEYAEVIEIDLDQLSEPVLCAPNDPDDARLLSEVAGQSIDEVFIGSCMTNIGHFRAA
CCCCCHHHHHEEEHHHCCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GKLLEKQPAGSLKTKLWLAPPTKMDQHQLTEEGYYGIYGRAGARMEMPGCSLCMGNQARV
HHHHHCCCCCCCCEEEEECCCCCCHHHHHHCCCCEEEEECCCCEECCCCCCEEECCCCCC
AAKSTVVSTSTRNFPNRLGDGADVFLASAELAAVAAVEGRLPSVDEYRRYMSEFDAMAGE
HHHHHEEECCCCCCHHHCCCCCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
IYRYMNFHEIEEFQNAASNVIPVAEEA
HHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLEAYRQHVEERAQAGVPPKPLNAEQTAALVKLLKNPPAGEEEAILDLLTNRVPPGVDEA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCH
AYVKAGFLTAIAKGEAESPLIDRVHAVKLLGTMQGGYNIVSLVELLDDDDLAREVGEQLK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHH
HTLLMFDAFHDVEERAKAGNAVAKEVIASWAEAEWFLAKPKLADKITLTVFKVPGETNTD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEEECCCCCCCC
DLSPAPDAWSRPDIPLHANAMLKNPRDGIEPVEPGVTGPLKQIEEVKAKGHPVVYVGDVV
CCCCCCCCCCCCCCCEECHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCEE
GTGSSRKSATNSVLWFFGDDIPNVPNKRAGGFCFGGKIAPIFFNTMEDSGALPIEMDVEK
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEEEEEEEEECCCCCCCCEEECHHH
LEMGDVIDVYPYEGKVCKHGTDEVLTTFELKTQLLLDEARAGGRIPLIIGRGLTAKARES
HCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
LGLAPSDVFRLPEQPADTGRGFTLAQKMVGKACGMDGVRPGMYCEPKMTTVGSQDTTGPM
CCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCC
TRDELKDLACLGFQADLVMQSFCHTSAYPKPVDVETHHTLPDFIMNRGGVSLRPGDGIIH
CHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCEECCCCCHHH
SWLNRMLLPDTVGTGGDSHTRFPMGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKR
HHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCEEHHHHCCCCCCCCCHHHHHEECCCC
QPGVTLRDLVHAIPYYAIQQGLLTVEKSGKKNAFSGRVLEIEGLEDLTVEQAFELSDASA
CCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCC
ERSAAGCTITLSEDSVSEYLKSNITLLKWMIANGYGDARTIERRIKGMEEWLENPSLMRA
HHCCCCEEEEECCHHHHHHHHCCCCEEEHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEC
DKDAEYAEVIEIDLDQLSEPVLCAPNDPDDARLLSEVAGQSIDEVFIGSCMTNIGHFRAA
CCCCCHHHHHEEEHHHCCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GKLLEKQPAGSLKTKLWLAPPTKMDQHQLTEEGYYGIYGRAGARMEMPGCSLCMGNQARV
HHHHHCCCCCCCCEEEEECCCCCCHHHHHHCCCCEEEEECCCCEECCCCCCEEECCCCCC
AAKSTVVSTSTRNFPNRLGDGADVFLASAELAAVAAVEGRLPSVDEYRRYMSEFDAMAGE
HHHHHEEECCCCCCHHHCCCCCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
IYRYMNFHEIEEFQNAASNVIPVAEEA
HHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]