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The map label for this gene is glcD [H]

Identifier: 161897779

GI number: 161897779

Start: 3009901

End: 3011400

Strand: Direct

Name: glcD [H]

Synonym: Csal_2687

Alternate gene names: 161897779

Gene position: 3009901-3011400 (Clockwise)

Preceding gene: 92114804

Following gene: 92114806

Centisome position: 81.42

GC content: 65.47

Gene sequence:

>1500_bases
ATGAACATCCTCTTTGACGAACGCCTGGACGGCACGCTGCCGGCGGTCGACAAGGATGCCCTTGTCACGCATCTGACCGC
GCACCTCCCCGACATGACGCTACTGCATCGCGAGGAGGACCTGCGTCCCTTCGAATGCGACGGCCTCTCGGCATATCGCG
TGCTACCCATGCTCGTGGCACTACCCGATACCCTGGAGCAGGTGGAAGCCCTGCTCGGCATCTGTCACACCCAGGGCGTT
CCGGTGGTCACGCGCGGCGCAGGCACCGGCCTCTCCGGTGGCGCCCTGCCCCTCGAAAAGGGAGTGCTACTGGTGATGTC
CCGCTTCAGCCGCATTCTCGACATCGACCCCGAGGCCCGCACCGCACGCGTGCAACCCGGTGTGCGCAACCTGGCGATTT
CCGAGGCGGCTGCGCCCCATGGGCTGTATTACGCACCCGACCCTTCGTCGCAGATCGCCTGCTCGATCGGCGGCAACGTG
GCCGAGAACGCCGGGGGCGTGCACTGCCTCAAGTACGGGCTGACCGTGCACAACGTGCTCGCGGTGAACATCCTGACCAT
CGAAGGGGAGCACATCACGCTCGGCAGCGAGGCCCTGGATACGCCGGGCTTCGATCTGCTGGCGCTGTTCACGGGCTCCG
AAGGCATGCTCGGCGTGATCACCGAAATCACCGTCAAGCTGCTGCCCAAGCCGGAAAGCGCCAAGGTACTGATGGCCAGC
TTCGACGACGTCGAGAAGGCCGGCAAGGCAGTGGGCGACATCATCGCCGCGGGCATCATTCCCGGCGGCCTGGAAATGAT
GGACCGGCTGGCCATTCAAGCCGCCGAGAACTTCGTCAAGGCCGGCTACCCGGTGGAGGCCGAGGCCATCCTGCTGTGCG
AACTGGATGGCGTGGAAGCCGACGTTCACGATGACTGCGAAACGGTTCGCCGAGTGCTCGAGGCCGCCGGCGCGACCGAT
ATTCGGCTGGCCCGTGACGACGCCGAGCGCGCCACCTTCTGGGCCGGACGCAAGAACGCCTTCCCCGCGGTAGGACGCAT
GTCGCCCGACTACTATTGCATGGACGGCACCATTCCCCGGCGCGAGCTGCCGCGCGTCCTCAAGGGCATCGCCGACCTCT
CGGCAACCCATGGCCTGCGCGTGGCCAATGTCTTCCATGCCGGCGACGGCAACATGCACCCGCTGATCCTGTTCGATGCC
AATGCGCCGGGAGAACTGGCACTGGCCGAGGAAGTGGGTGGCAAGATCCTCGAACTCTGCGTCGAGGTCGGCGGCAGCAT
TACCGGCGAGCATGGCGTGGGACGCGAGAAGATCAACCAGATGTGCGCCCAGTTCTCCCCCGACGAGATCACGGTCTTTC
ATGCCGTCAAGGCCGCCTTCGACCCGCTCGGGCTGCTCAATCCCGGCAAGAACATTCCGACGCTGGCACGCTGCGCCGAA
TTCGGTGCCATGCACGTGCATCACGGCAACCTGCCCCACCCCGAGTTGCCGCGTTTCTAA

Upstream 100 bases:

>100_bases
GCCATAAGAGAATATGGAAACACTTTCCATAAGCGGCGAGGTCCGGCGCCATGCGCTGACGCTCTCGCCCACGCGTGGCG
GCATCGCCCAAGGAGACCGC

Downstream 100 bases:

>100_bases
CGGCATGCGTGCGAAGGAGACAACATGACGCATCCACAGGATCAACATCCGCCACAGGACGCACGCACCTTGCCGACGCG
CGATCACGATGCCAGCGAGG

Product: glycolate oxidase subunit GlcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MNILFDERLDGTLPAVDKDALVTHLTAHLPDMTLLHREEDLRPFECDGLSAYRVLPMLVALPDTLEQVEALLGICHTQGV
PVVTRGAGTGLSGGALPLEKGVLLVMSRFSRILDIDPEARTARVQPGVRNLAISEAAAPHGLYYAPDPSSQIACSIGGNV
AENAGGVHCLKYGLTVHNVLAVNILTIEGEHITLGSEALDTPGFDLLALFTGSEGMLGVITEITVKLLPKPESAKVLMAS
FDDVEKAGKAVGDIIAAGIIPGGLEMMDRLAIQAAENFVKAGYPVEAEAILLCELDGVEADVHDDCETVRRVLEAAGATD
IRLARDDAERATFWAGRKNAFPAVGRMSPDYYCMDGTIPRRELPRVLKGIADLSATHGLRVANVFHAGDGNMHPLILFDA
NAPGELALAEEVGGKILELCVEVGGSITGEHGVGREKINQMCAQFSPDEITVFHAVKAAFDPLGLLNPGKNIPTLARCAE
FGAMHVHHGNLPHPELPRF

Sequences:

>Translated_499_residues
MNILFDERLDGTLPAVDKDALVTHLTAHLPDMTLLHREEDLRPFECDGLSAYRVLPMLVALPDTLEQVEALLGICHTQGV
PVVTRGAGTGLSGGALPLEKGVLLVMSRFSRILDIDPEARTARVQPGVRNLAISEAAAPHGLYYAPDPSSQIACSIGGNV
AENAGGVHCLKYGLTVHNVLAVNILTIEGEHITLGSEALDTPGFDLLALFTGSEGMLGVITEITVKLLPKPESAKVLMAS
FDDVEKAGKAVGDIIAAGIIPGGLEMMDRLAIQAAENFVKAGYPVEAEAILLCELDGVEADVHDDCETVRRVLEAAGATD
IRLARDDAERATFWAGRKNAFPAVGRMSPDYYCMDGTIPRRELPRVLKGIADLSATHGLRVANVFHAGDGNMHPLILFDA
NAPGELALAEEVGGKILELCVEVGGSITGEHGVGREKINQMCAQFSPDEITVFHAVKAAFDPLGLLNPGKNIPTLARCAE
FGAMHVHHGNLPHPELPRF
>Mature_499_residues
MNILFDERLDGTLPAVDKDALVTHLTAHLPDMTLLHREEDLRPFECDGLSAYRVLPMLVALPDTLEQVEALLGICHTQGV
PVVTRGAGTGLSGGALPLEKGVLLVMSRFSRILDIDPEARTARVQPGVRNLAISEAAAPHGLYYAPDPSSQIACSIGGNV
AENAGGVHCLKYGLTVHNVLAVNILTIEGEHITLGSEALDTPGFDLLALFTGSEGMLGVITEITVKLLPKPESAKVLMAS
FDDVEKAGKAVGDIIAAGIIPGGLEMMDRLAIQAAENFVKAGYPVEAEAILLCELDGVEADVHDDCETVRRVLEAAGATD
IRLARDDAERATFWAGRKNAFPAVGRMSPDYYCMDGTIPRRELPRVLKGIADLSATHGLRVANVFHAGDGNMHPLILFDA
NAPGELALAEEVGGKILELCVEVGGSITGEHGVGREKINQMCAQFSPDEITVFHAVKAAFDPLGLLNPGKNIPTLARCAE
FGAMHVHHGNLPHPELPRF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=426, Percent_Identity=30.9859154929577, Blast_Score=199, Evalue=7e-51,
Organism=Homo sapiens, GI37595754, Length=449, Percent_Identity=30.2895322939866, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI119964728, Length=447, Percent_Identity=29.5302013422819, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI4501993, Length=254, Percent_Identity=25.9842519685039, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1789351, Length=499, Percent_Identity=77.1543086172345, Blast_Score=795, Evalue=0.0,
Organism=Escherichia coli, GI1787977, Length=502, Percent_Identity=24.9003984063745, Blast_Score=106, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71992373, Length=471, Percent_Identity=26.963906581741, Blast_Score=180, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI71992381, Length=473, Percent_Identity=26.4270613107822, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71992392, Length=359, Percent_Identity=27.5766016713092, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17534361, Length=457, Percent_Identity=26.2582056892779, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17556096, Length=461, Percent_Identity=21.2581344902386, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320023, Length=458, Percent_Identity=29.0393013100437, Blast_Score=179, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6320027, Length=433, Percent_Identity=27.2517321016166, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320764, Length=458, Percent_Identity=26.8558951965066, Blast_Score=156, Evalue=6e-39,
Organism=Drosophila melanogaster, GI18921117, Length=469, Percent_Identity=28.1449893390192, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24639277, Length=469, Percent_Identity=28.1449893390192, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24639275, Length=469, Percent_Identity=28.1449893390192, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24653753, Length=221, Percent_Identity=26.2443438914027, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 53108; Mature: 53108

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILFDERLDGTLPAVDKDALVTHLTAHLPDMTLLHREEDLRPFECDGLSAYRVLPMLVA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
LPDTLEQVEALLGICHTQGVPVVTRGAGTGLSGGALPLEKGVLLVMSRFSRILDIDPEAR
CCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCHHCCHHHHHHHHHHHCCCCCCCC
TARVQPGVRNLAISEAAAPHGLYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNVL
EEECCCCHHHHHHHHCCCCCCEEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCEEEEE
AVNILTIEGEHITLGSEALDTPGFDLLALFTGSEGMLGVITEITVKLLPKPESAKVLMAS
EEEEEEEECCEEEECCHHCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEC
FDDVEKAGKAVGDIIAAGIIPGGLEMMDRLAIQAAENFVKAGYPVEAEAILLCELDGVEA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCC
DVHDDCETVRRVLEAAGATDIRLARDDAERATFWAGRKNAFPAVGRMSPDYYCMDGTIPR
CCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHEECCCCCCCCCCCCCCCCEEEECCCCCH
RELPRVLKGIADLSATHGLRVANVFHAGDGNMHPLILFDANAPGELALAEEVGGKILELC
HHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCEEEEECCCCCCCEEEHHHHCHHHHHHH
VEVGGSITGEHGVGREKINQMCAQFSPDEITVFHAVKAAFDPLGLLNPGKNIPTLARCAE
HHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
FGAMHVHHGNLPHPELPRF
HCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
MNILFDERLDGTLPAVDKDALVTHLTAHLPDMTLLHREEDLRPFECDGLSAYRVLPMLVA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
LPDTLEQVEALLGICHTQGVPVVTRGAGTGLSGGALPLEKGVLLVMSRFSRILDIDPEAR
CCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCHHCCHHHHHHHHHHHCCCCCCCC
TARVQPGVRNLAISEAAAPHGLYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNVL
EEECCCCHHHHHHHHCCCCCCEEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCEEEEE
AVNILTIEGEHITLGSEALDTPGFDLLALFTGSEGMLGVITEITVKLLPKPESAKVLMAS
EEEEEEEECCEEEECCHHCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEC
FDDVEKAGKAVGDIIAAGIIPGGLEMMDRLAIQAAENFVKAGYPVEAEAILLCELDGVEA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCC
DVHDDCETVRRVLEAAGATDIRLARDDAERATFWAGRKNAFPAVGRMSPDYYCMDGTIPR
CCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHEECCCCCCCCCCCCCCCCEEEECCCCCH
RELPRVLKGIADLSATHGLRVANVFHAGDGNMHPLILFDANAPGELALAEEVGGKILELC
HHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCEEEEECCCCCCCEEEHHHHCHHHHHHH
VEVGGSITGEHGVGREKINQMCAQFSPDEITVFHAVKAAFDPLGLLNPGKNIPTLARCAE
HHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
FGAMHVHHGNLPHPELPRF
HCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]