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The map label for this gene is fumA [H]

Identifier: 161870464

GI number: 161870464

Start: 1545513

End: 1547036

Strand: Direct

Name: fumA [H]

Synonym: NMCC_1519

Alternate gene names: 161870464

Gene position: 1545513-1547036 (Clockwise)

Preceding gene: 161870463

Following gene: 161870465

Centisome position: 71.77

GC content: 56.96

Gene sequence:

>1524_bases
ATGACCGTCATCAAACAGGAAGACTTTATCCAAAGCATTTGCGATGCCTTCCAATTCATCAGCTACTACCATCCCAAAGA
CTACATCGACGCGCTCTATAAAGCGTGGCAGAAGGAAGAAAACCCCGCCGCCAAAGACGCGATGACGCAGATTTTGGTCA
ACAGCCGCATGTGTGCCGAAAACAACCGCCCCATCTGCCAAGACACAGGTATCGCAACCGTGTTTTTGAAAGTCGGTATG
GATGTGCAGTGGGATGCGGACATGAGCGTGGAAGAAATGGTTAACGAAGGCGTGCGCCGCGCCTACACTTGGGAAGGCAA
CACCCTGCGCGCCTCTGTCCTCGCCGATCCGGCCGGCAAACGTCAAAACACCAAAGACAACACGCCCGCCGTCATCCACA
TGAGCATCGTGCCGGGCGGTAAAGTCGAAGTAACCTGCGCGGCAAAAGGCGGCGGTTCTGAAAACAAATCCAAACTCGCC
ATGCTCAACCCTTCCGACAACATCGTCGATTGGGTATTGAAAACCATTCCGACGATGGGCGCGGGCTGGTGTCCTCCCGG
CATCTTGGGCATCGGCATCGGCGGCACGCCTGAAAAAGCCGTGTTGATGGCAAAAGAGTCCCTGATGAGCCACATCGACA
TCCAAGAATTGCAGGAAAAAGCCGCGTCCGGCGCAGAATTATCCACCACCGAAGCCCTGCGCCTCGAACTCTTTGAAAAA
GTCAACGCGCTGGGCATCGGCGCACAAGGCTTGGGCGGACTGACCACCGTGTTGGACGTGAAAATCCTCGATTATCCGAC
CCATGCCGCCTCCAAACCGATTGCCATGATTCCGAACTGCGCCGCCACCCGCCACGTCGAGTTCGAATTGGACGGCTCAG
GCCCTGTCGAACTCACGCCGCCGCGCGTCGAAGACTGGCCCGATTTGACTTACAGCCCCGACAACGGCAAACGCGTCGAT
GTCGACAAGCTGACCAAAGAAGAAGTGGCAAGCTGGAAAACCGGCGACGTATTGCTGTTGAACGGCAAAATCCTCACCGG
CCGCGATGCCGCACACAAACGCCTCGTCGATATGCTCAACAAAGGCGAAGAATTGCCCGTCGATTTCACCAACCGCCTGA
TTTACTACGTTGGTCCCGTCGATCCGGTCGGCGATGAAGTCGTCGGCCCGGCAGGCCCCACCACCGCCACCCGCATGGAC
AAATTCACCCGCCAAATGCTCGAACAAACCGACCTCTTGGGCATGATCGGCAAATCCGAACGCGGCGCGGCCACCTGCGA
AGCCATCGCCGACAACAAAGCCGTGTACCTGATGGCAGTCGGCGGCGCGGCGTATCTCGTGGCAAAAGCCATCAAATCTT
CCAAAGTCTTGGCGTTCCCCGAATTGGGCATGGAAGCCATTTACGAATTTGAAGTCAAAGACATGCCCGTAACCGTCGCC
GTAGACAGCAAAGGCGAATCCATCCACGCCACCGCCCCGCCCCAATGGCAGGCGAAAATCGGCATCATCCCCGTCGAATC
TTGA

Upstream 100 bases:

>100_bases
ACGCTGGCACTATTTCAACCTGATAAAAAACAGCCTTCAAAAAGGTTGCTTAAAACAGCAGCAGACACTTACCGCCACAA
CCTTGAAAAGGAACACAATC

Downstream 100 bases:

>100_bases
GGCGCCATGCCGTCTGAACACAAAATCTGCTTTCAGACGGCATTTCCGCCCCTGGTTGCGGTACAATCCACCATTTCATC
ACTCGGCGACCCACACCGTG

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAAKDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGM
DVQWDADMSVEEMVNEGVRRAYTWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGGKVEVTCAAKGGGSENKSKLA
MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQEKAASGAELSTTEALRLELFEK
VNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAMIPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVD
VDKLTKEEVASWKTGDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAGPTTATRMD
KFTRQMLEQTDLLGMIGKSERGAATCEAIADNKAVYLMAVGGAAYLVAKAIKSSKVLAFPELGMEAIYEFEVKDMPVTVA
VDSKGESIHATAPPQWQAKIGIIPVES

Sequences:

>Translated_507_residues
MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAAKDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGM
DVQWDADMSVEEMVNEGVRRAYTWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGGKVEVTCAAKGGGSENKSKLA
MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQEKAASGAELSTTEALRLELFEK
VNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAMIPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVD
VDKLTKEEVASWKTGDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAGPTTATRMD
KFTRQMLEQTDLLGMIGKSERGAATCEAIADNKAVYLMAVGGAAYLVAKAIKSSKVLAFPELGMEAIYEFEVKDMPVTVA
VDSKGESIHATAPPQWQAKIGIIPVES
>Mature_506_residues
TVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAAKDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMD
VQWDADMSVEEMVNEGVRRAYTWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGGKVEVTCAAKGGGSENKSKLAM
LNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQEKAASGAELSTTEALRLELFEKV
NALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAMIPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDV
DKLTKEEVASWKTGDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAGPTTATRMDK
FTRQMLEQTDLLGMIGKSERGAATCEAIADNKAVYLMAVGGAAYLVAKAIKSSKVLAFPELGMEAIYEFEVKDMPVTVAV
DSKGESIHATAPPQWQAKIGIIPVES

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=452, Percent_Identity=32.0796460176991, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1787897, Length=493, Percent_Identity=31.8458417849899, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1789443, Length=179, Percent_Identity=31.8435754189944, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789442, Length=269, Percent_Identity=26.0223048327138, Blast_Score=85, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54864; Mature: 54733

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAAKDAMTQILVNSRMCAE
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHCC
NNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAYTWEGNTLRASVLADPAGK
CCCCCCCCCCCEEEEEEECCCEEECCCCCHHHHHHHHHHEEEEECCCEEEEEEEECCCCC
RQNTKDNTPAVIHMSIVPGGKVEVTCAAKGGGSENKSKLAMLNPSDNIVDWVLKTIPTMG
CCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHCCCC
AGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQEKAASGAELSTTEALRLELFEK
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHH
VNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAMIPNCAATRHVEFELDGSGPVELTP
HHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCCEEECCCCCCCEEEEEEECCCCCEEECC
PRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKTGDVLLLNGKILTGRDAAHKRLVDMLN
CCCCCCCCCCCCCCCCCEECHHHHHHHHHHCCCCCCEEEECCEEEECCHHHHHHHHHHHC
KGEELPVDFTNRLIYYVGPVDPVGDEVVGPAGPTTATRMDKFTRQMLEQTDLLGMIGKSE
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
RGAATCEAIADNKAVYLMAVGGAAYLVAKAIKSSKVLAFPELGMEAIYEFEVKDMPVTVA
CCCHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCEEECHHCCCHHEEEEEECCCCEEEE
VDSKGESIHATAPPQWQAKIGIIPVES
ECCCCCEEECCCCCCCCEEEEEEECCC
>Mature Secondary Structure 
TVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAAKDAMTQILVNSRMCAE
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHCC
NNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAYTWEGNTLRASVLADPAGK
CCCCCCCCCCCEEEEEEECCCEEECCCCCHHHHHHHHHHEEEEECCCEEEEEEEECCCCC
RQNTKDNTPAVIHMSIVPGGKVEVTCAAKGGGSENKSKLAMLNPSDNIVDWVLKTIPTMG
CCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHCCCC
AGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQEKAASGAELSTTEALRLELFEK
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHH
VNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAMIPNCAATRHVEFELDGSGPVELTP
HHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCCEEECCCCCCCEEEEEEECCCCCEEECC
PRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKTGDVLLLNGKILTGRDAAHKRLVDMLN
CCCCCCCCCCCCCCCCCEECHHHHHHHHHHCCCCCCEEEECCEEEECCHHHHHHHHHHHC
KGEELPVDFTNRLIYYVGPVDPVGDEVVGPAGPTTATRMDKFTRQMLEQTDLLGMIGKSE
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
RGAATCEAIADNKAVYLMAVGGAAYLVAKAIKSSKVLAFPELGMEAIYEFEVKDMPVTVA
CCCHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCEEECHHCCCHHEEEEEECCCCEEEE
VDSKGESIHATAPPQWQAKIGIIPVES
ECCCCCEEECCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]