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The map label for this gene is hisJ [H]

Identifier: 161870463

GI number: 161870463

Start: 1544400

End: 1545206

Strand: Direct

Name: hisJ [H]

Synonym: NMCC_1518

Alternate gene names: 161870463

Gene position: 1544400-1545206 (Clockwise)

Preceding gene: 161870462

Following gene: 161870464

Centisome position: 71.72

GC content: 51.55

Gene sequence:

>807_bases
ATGAATATGAAAAAATGGATTGCCGCCGCCCTTGCCTGTTCCGCGCTCGCGCTGTCTGCCTGCGGCGGTCAGGGCAAAGA
TGCCGCCGCGCCCGCCGCAAACCCCGACAAAGTGTACCGCGTGGCTTCCAACGCCGAGTTTGCCCCCTTTGAATCTTTAG
ACTCGAAAGGCAATGTCGAAGGTTTCGATGTGGATTTGATGAACGCGATGGCGAAGGCGGGCAATTTTAAAATCGAATTC
AAACACCAGCCGTGGGACAGCCTTTTCCCCGCCTTGAACAACGGCGATGCGGACGTTGTGATGTCGGGCGTAACCATTAC
CGACGACCGCAAACAGTCTATGGACTTCAGCGACCCGTATTTTGAAATCACCCAAGTCGTCCTCGTTCCGAAAGGCAAAA
AAATATCTTCTTCCGACGATTTGAAAAACATGAACAAAGTCGGCGTGGTAACCGGCTACACGGGCGATTTCTCCGTTTCC
AAACTCTTGGGCAACGACAACCCGAAAATCGCGCGCTTTGAAAACGTTCCCCTGATTATCAAAGAACTGGAAAACGGCGG
CTTGGATTCCGTGGTCAGCGACAGCGCGGTCATCGCCAATTATGTGAAAAACAATCCGACCAAAGGAATGGACTTCGTTA
CCCTGCCCGACTTCACCACCGAACACTACGGCATCGCGGTACGCAAAGGCGACGAAGCAACCGTCAAAATGCTGAACGAT
GCGTTGAAAAAAGTACGCGAAAGCGGCGAATACGACAAAATCTACGCCAAATATTTTGCAAAAGAAAACGGACAGGCCGC
AAAATAA

Upstream 100 bases:

>100_bases
TTTGTTCCACGACGTTACTTATGCGTTTTTCCGTGAAAAAGAACGTTAATTTTATGTTAAACTGATTTTTTAGGCTTTTT
GATTACCGAAAGGAATTTTG

Downstream 100 bases:

>100_bases
GCCCGCCCATCCGAACACAATGCCGTCTGAAGCCCTTTCAGACGGCATTGTTCATCAATCGGCCTACAATGAACTGCCTG
CTGATTTCTCCCTACCGCAA

Product: polar amino acid transport system substrate-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASNAEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEF
KHQPWDSLFPALNNGDADVVMSGVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDFTTEHYGIAVRKGDEATVKMLND
ALKKVRESGEYDKIYAKYFAKENGQAAK

Sequences:

>Translated_268_residues
MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASNAEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEF
KHQPWDSLFPALNNGDADVVMSGVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDFTTEHYGIAVRKGDEATVKMLND
ALKKVRESGEYDKIYAKYFAKENGQAAK
>Mature_268_residues
MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASNAEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEF
KHQPWDSLFPALNNGDADVVMSGVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDFTTEHYGIAVRKGDEATVKMLND
ALKKVRESGEYDKIYAKYFAKENGQAAK

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787088, Length=266, Percent_Identity=34.5864661654135, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1788649, Length=252, Percent_Identity=30.952380952381, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787085, Length=222, Percent_Identity=31.981981981982, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787031, Length=265, Percent_Identity=32.4528301886792, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788648, Length=231, Percent_Identity=29.004329004329, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788228, Length=223, Percent_Identity=30.0448430493274, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786876, Length=238, Percent_Identity=27.7310924369748, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29015; Mature: 29015

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASNAEFAPFESLDSKGNVE
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEEECCCCCCCHHHCCCCCCCC
GFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMSGVTITDDRKQSMDFSDPY
CCCHHHHHHHHHCCCEEEEEECCCHHHHCCCCCCCCHHEEEECEEEECCHHHCCCCCCCH
FEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVSKLLGNDNPKIARFENVPLII
HEEEEEEEECCCCCCCCCHHHHCCCCEEEEEECCCCCHHHHHHCCCCCCEEEECCCCEEE
KELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDFTTEHYGIAVRKGDEATVKMLND
EECCCCCCCHHHCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEECCCHHHHHHHHH
ALKKVRESGEYDKIYAKYFAKENGQAAK
HHHHHHHCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASNAEFAPFESLDSKGNVE
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEEECCCCCCCHHHCCCCCCCC
GFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMSGVTITDDRKQSMDFSDPY
CCCHHHHHHHHHCCCEEEEEECCCHHHHCCCCCCCCHHEEEECEEEECCHHHCCCCCCCH
FEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVSKLLGNDNPKIARFENVPLII
HEEEEEEEECCCCCCCCCHHHHCCCCEEEEEECCCCCHHHHHHCCCCCCEEEECCCCEEE
KELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDFTTEHYGIAVRKGDEATVKMLND
EECCCCCCCHHHCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEECCCHHHHHHHHH
ALKKVRESGEYDKIYAKYFAKENGQAAK
HHHHHHHCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1448614 [H]