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The map label for this gene is pilR [H]

Identifier: 161870457

GI number: 161870457

Start: 1539147

End: 1539614

Strand: Direct

Name: pilR [H]

Synonym: NMCC_1512

Alternate gene names: 161870457

Gene position: 1539147-1539614 (Clockwise)

Preceding gene: 161870456

Following gene: 161870458

Centisome position: 71.47

GC content: 57.48

Gene sequence:

>468_bases
ATGACTGAACTGCAACACCCCGTCCTCGTCGTCGATGACGAAACCGACATTCTCGACCTGATGGAAATGACCCTGATGAA
AATGGGCTTGCGCGTCCATACCGCGTCAGGCGTTGCCGAAGCCAAAAACAAGCTCGACAGCCAACGCTATTCGCTCGTCC
TGACCGATATGCGTATGCCGGACAGCTCGGGGCTGGAAGTCGTCCAACACATCAACAGCCGCCTGCTCGATACGCCGGTT
GCCGTCATCACCGCCTTCGGCAACGCCGATCAGGCACAGGAAGCGTTGCGTTGCGGCGCGTTCGACCCCGATACCATGCA
GATACAGGACTATCTCGACCAAATCGAACGCGACATCATCGAACAAACCCTCAAACAAACCGAAGGCAACCGCACGCAGG
CCGCCAAACGCTTGGGCATCAGCTTCCGTTCCATGCGCTACCGTATGGAACGCCTCAACATCGGCTGA

Upstream 100 bases:

>100_bases
GGGCTGTATGTCGCCCGCGAACTGGCGCACGCCAATTTCGGCGATTTGACCTACCTACCGGAAGCCAAATGTTTCGAACT
CACATTACCGGAAAAAACCA

Downstream 100 bases:

>100_bases
CGACAAAACGGCATCCGCACCATCTCCGCCCACCCGAAAAAATGCCGTCTGAAACGGCACGGGAAAGCGGGTTCGCCCCA
CGCCCGAACGGACACAAAAC

Product: two component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNKLDSQRYSLVLTDMRMPDSSGLEVVQHINSRLLDTPV
AVITAFGNADQAQEALRCGAFDPDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG

Sequences:

>Translated_155_residues
MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNKLDSQRYSLVLTDMRMPDSSGLEVVQHINSRLLDTPV
AVITAFGNADQAQEALRCGAFDPDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG
>Mature_154_residues
TELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNKLDSQRYSLVLTDMRMPDSSGLEVVQHINSRLLDTPVA
VITAFGNADQAQEALRCGAFDPDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=95, Percent_Identity=33.6842105263158, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1790299, Length=114, Percent_Identity=35.0877192982456, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 17542; Mature: 17411

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
6.5 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNKLDSQRYSLVLTDMRMP
CCCCCCCEEEECCCCHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHEEEECCCCC
DSSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFDPDTMQIQDYLDQIERDII
CCCCHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
EQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNKLDSQRYSLVLTDMRMP
CCCCCCEEEECCCCHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHEEEECCCCC
DSSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFDPDTMQIQDYLDQIERDII
CCCCHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
EQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]