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The map label for this gene is pilS [H]

Identifier: 161870456

GI number: 161870456

Start: 1537578

End: 1539095

Strand: Direct

Name: pilS [H]

Synonym: NMCC_1511

Alternate gene names: 161870456

Gene position: 1537578-1539095 (Clockwise)

Preceding gene: 161870455

Following gene: 161870457

Centisome position: 71.4

GC content: 58.17

Gene sequence:

>1518_bases
ATGGTAATTTCCAATACCCGCGAACTTGAGAAACTCAAAGACCGGATTCCCAATCTGATCAACATCGCCCGCATCGCCAT
CGTCTTCCCCCTGCTGATTATGCATATCTTCGGGTTGGAGGTCAGCGGACATGAAAACCTGCACGCTTCGTGGACGGCGT
GGGCATTTTATGTTTGGCTCGCCATTTCCTGCTGGCTGATTTTCTTTTCCATCATCCATCCGCATTGGCAATGGCAGTCG
CTGAAAATGCCGCGTTTCAGCGCGGTGGCGGACATCACGATGATCGGCGTGCTGACCTACCTGTTCGGCGGCATCGATTC
CGGCTTCGGCATCCTGATTCTGCCCTTCGTCGTCTGCTCCTGCCTGCTCAGCTACGGGCGTTACCCCCTGCTCTATGCCA
GCTACGCCGCCATCCTGCTGATATTCAACGCCATTGCCGACGGCGATATCGGCAAATATCCGCACATATCGGATGCCCGA
ACCGCCTCGGCAACCTTCATCCTCGTCGCCGCCTCCTATCTTTCCGCCATCTTCACCTCACTGTCGGTCAGATACATCGA
CAGCGCCGGCCAACTGGCCCGCGACAACCACCTCGCCTACCGGCGCATCAAAGGCTTGAGCCAAACCGTACTCGAACGCG
TTCAAGAAGCCGTCGTCGTCATCAATACCGAAGGGCTGGCGGTGCTGTTCAACCGGAAGGCGAAAGACCTTTTCCCCGCA
CTCGAAATCGGACGGCGCGCCGACCTGTCCGATTCTGCCGCCGAACTGTGGGACCAAGCCTCTCCGCACACTTTCGAATA
CGTCCTCGGCACACTCGGCCTGACCGCCCGCATCCGCGCCGTGCCGATGAACAAAGAGCCGGACAAGCTGCTTATCCTCT
ACATCCGCCCGCAAAGCGAAATTCAGGCAGAAGCCCTGTCCGTCAAACTTGCCGCGCTCGGACAACTGACCGCCAACCTT
GCCCACGAAATCCGCAACCCGATGTCCGCCATCCGCCACGCCAACGACCTGCTGCGCGAAAATATGGAAGCGGGGGCGGC
AGATCCGTTCAACGCCAAATTGTGCAAAATCATCGACGGCAACGTCTGCCGCATCGACAAAATGCTCGAAGACATTTCCT
CACTCAACAAGCGCAACAAAACCGAACGCGAAACCATCGGCCTGATACCGTTTTGGGAAGAATTCAAACAAGAGTTCCTG
CTCGGCCATCCCGATGCCGCCGGCTGCATCCGCTTGGATATGCAGGGCAATCACCCGACCGCCTATTTCGATCCCGCCCA
CCTGCGGCAGATTATGTGGAATCTCGCCAACAACGCGTGGCGGCACAGCCGCAAACAGCCCGGCTCGATTTCCGTCACCA
TCCGCCCCGCGCAAAAAAACACCGTCTGCATCCTCTTTGCCGACCGCCCGAAGTGCAGGAACACCTGTTCGAACCCTTTT
ACACCACGGCGGAAAACGGCACCGGCCTCGGGCTGTATGTCGCCCGCGAACTGGCGCACGCCAATTTCGGCGATTTGA

Upstream 100 bases:

>100_bases
CTGCTTGAGGCAAAACGCGGCAAAAAAGGCAAACTATTACCCATATCGGGCAGCCTGAAACAGCTTTCTTCCCCGAAATA
AACCCGACTCTGCACATACT

Downstream 100 bases:

>100_bases
CCTACCTACCGGAAGCCAAATGTTTCGAACTCACATTACCGGAAAAAACCAATGACTGAACTGCAACACCCCGTCCTCGT
CGTCGATGACGAAACCGACA

Product: two component sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MVISNTRELEKLKDRIPNLINIARIAIVFPLLIMHIFGLEVSGHENLHASWTAWAFYVWLAISCWLIFFSIIHPHWQWQS
LKMPRFSAVADITMIGVLTYLFGGIDSGFGILILPFVVCSCLLSYGRYPLLYASYAAILLIFNAIADGDIGKYPHISDAR
TASATFILVAASYLSAIFTSLSVRYIDSAGQLARDNHLAYRRIKGLSQTVLERVQEAVVVINTEGLAVLFNRKAKDLFPA
LEIGRRADLSDSAAELWDQASPHTFEYVLGTLGLTARIRAVPMNKEPDKLLILYIRPQSEIQAEALSVKLAALGQLTANL
AHEIRNPMSAIRHANDLLRENMEAGAADPFNAKLCKIIDGNVCRIDKMLEDISSLNKRNKTERETIGLIPFWEEFKQEFL
LGHPDAAGCIRLDMQGNHPTAYFDPAHLRQIMWNLANNAWRHSRKQPGSISVTIRPAQKNTVCILFADRPKCRNTCSNPF
TPRRKTAPASGCMSPANWRTPISAI

Sequences:

>Translated_505_residues
MVISNTRELEKLKDRIPNLINIARIAIVFPLLIMHIFGLEVSGHENLHASWTAWAFYVWLAISCWLIFFSIIHPHWQWQS
LKMPRFSAVADITMIGVLTYLFGGIDSGFGILILPFVVCSCLLSYGRYPLLYASYAAILLIFNAIADGDIGKYPHISDAR
TASATFILVAASYLSAIFTSLSVRYIDSAGQLARDNHLAYRRIKGLSQTVLERVQEAVVVINTEGLAVLFNRKAKDLFPA
LEIGRRADLSDSAAELWDQASPHTFEYVLGTLGLTARIRAVPMNKEPDKLLILYIRPQSEIQAEALSVKLAALGQLTANL
AHEIRNPMSAIRHANDLLRENMEAGAADPFNAKLCKIIDGNVCRIDKMLEDISSLNKRNKTERETIGLIPFWEEFKQEFL
LGHPDAAGCIRLDMQGNHPTAYFDPAHLRQIMWNLANNAWRHSRKQPGSISVTIRPAQKNTVCILFADRPKCRNTCSNPF
TPRRKTAPASGCMSPANWRTPISAI
>Mature_505_residues
MVISNTRELEKLKDRIPNLINIARIAIVFPLLIMHIFGLEVSGHENLHASWTAWAFYVWLAISCWLIFFSIIHPHWQWQS
LKMPRFSAVADITMIGVLTYLFGGIDSGFGILILPFVVCSCLLSYGRYPLLYASYAAILLIFNAIADGDIGKYPHISDAR
TASATFILVAASYLSAIFTSLSVRYIDSAGQLARDNHLAYRRIKGLSQTVLERVQEAVVVINTEGLAVLFNRKAKDLFPA
LEIGRRADLSDSAAELWDQASPHTFEYVLGTLGLTARIRAVPMNKEPDKLLILYIRPQSEIQAEALSVKLAALGQLTANL
AHEIRNPMSAIRHANDLLRENMEAGAADPFNAKLCKIIDGNVCRIDKMLEDISSLNKRNKTERETIGLIPFWEEFKQEFL
LGHPDAAGCIRLDMQGNHPTAYFDPAHLRQIMWNLANNAWRHSRKQPGSISVTIRPAQKNTVCILFADRPKCRNTCSNPF
TPRRKTAPASGCMSPANWRTPISAI

Specific function: Member of the two-component regulatory system pilS/pilR involved in the regulation of the expression of type 4 fimbriae. PilS may function as a membrane-associated protein kinase that phosphorylates pilR in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56510; Mature: 56510

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVISNTRELEKLKDRIPNLINIARIAIVFPLLIMHIFGLEVSGHENLHASWTAWAFYVWL
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
AISCWLIFFSIIHPHWQWQSLKMPRFSAVADITMIGVLTYLFGGIDSGFGILILPFVVCS
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
CLLSYGRYPLLYASYAAILLIFNAIADGDIGKYPHISDARTASATFILVAASYLSAIFTS
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LSVRYIDSAGQLARDNHLAYRRIKGLSQTVLERVQEAVVVINTEGLAVLFNRKAKDLFPA
HHHHEECCCCCHHHCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECCCHHHHHHH
LEIGRRADLSDSAAELWDQASPHTFEYVLGTLGLTARIRAVPMNKEPDKLLILYIRPQSE
HHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCHHHEEEEECCCCCCCEEEEEEECCCHH
IQAEALSVKLAALGQLTANLAHEIRNPMSAIRHANDLLRENMEAGAADPFNAKLCKIIDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEECC
NVCRIDKMLEDISSLNKRNKTERETIGLIPFWEEFKQEFLLGHPDAAGCIRLDMQGNHPT
CHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCC
AYFDPAHLRQIMWNLANNAWRHSRKQPGSISVTIRPAQKNTVCILFADRPKCRNTCSNPF
CEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEECCCCCHHHCCCCCC
TPRRKTAPASGCMSPANWRTPISAI
CCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVISNTRELEKLKDRIPNLINIARIAIVFPLLIMHIFGLEVSGHENLHASWTAWAFYVWL
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
AISCWLIFFSIIHPHWQWQSLKMPRFSAVADITMIGVLTYLFGGIDSGFGILILPFVVCS
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
CLLSYGRYPLLYASYAAILLIFNAIADGDIGKYPHISDARTASATFILVAASYLSAIFTS
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LSVRYIDSAGQLARDNHLAYRRIKGLSQTVLERVQEAVVVINTEGLAVLFNRKAKDLFPA
HHHHEECCCCCHHHCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECCCHHHHHHH
LEIGRRADLSDSAAELWDQASPHTFEYVLGTLGLTARIRAVPMNKEPDKLLILYIRPQSE
HHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCHHHEEEEECCCCCCCEEEEEEECCCHH
IQAEALSVKLAALGQLTANLAHEIRNPMSAIRHANDLLRENMEAGAADPFNAKLCKIIDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEECC
NVCRIDKMLEDISSLNKRNKTERETIGLIPFWEEFKQEFLLGHPDAAGCIRLDMQGNHPT
CHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCC
AYFDPAHLRQIMWNLANNAWRHSRKQPGSISVTIRPAQKNTVCILFADRPKCRNTCSNPF
CEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEECCCCCHHHCCCCCC
TPRRKTAPASGCMSPANWRTPISAI
CCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8097014; 10984043 [H]