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The map label for this gene is acnB [H]

Identifier: 161870427

GI number: 161870427

Start: 1501985

End: 1504570

Strand: Direct

Name: acnB [H]

Synonym: NMCC_1475

Alternate gene names: 161870427

Gene position: 1501985-1504570 (Clockwise)

Preceding gene: 161870426

Following gene: 161870428

Centisome position: 69.75

GC content: 55.88

Gene sequence:

>2586_bases
ATGTTAGAAGCCTATCGTAAAGCCGCCGCCGAGCGCGCCGCCCTTGGTATTCCCGCCCTTCCTCTGACTGCTCAGCAAAC
CGCCGATTTGGTTGAGCTGCTGAAAAGCCCGCCCGCAGGCGAAGGCGAGTTCTTGGTCGAACTGCTTGCCCACCGTGTTC
CGCCCGGTGTGGACGATGCCGCCAAAGTCAAAGCCTCATTCCTGGCTGCCGTTGCCGAAGGCAGCGCGTCCAGCCCGCTG
ATCTCCCCCGAATATGCGACCGAACTCTTAGGTACGATGCTCGGCGGTTACAATATCCACGCCTTAATCGAACTCTTGGA
CGACGACAAACTCGCGCCCATTGCCGCCAAAGGCTTGAAACATACGCTTCTGATGTTCGATTCCTTCCACGACGTTCAGG
AAAAAGCCGAAAAAGGCAACAAATACGCGCAAGAAGTTTTGCAATCTTGGGCAGATGCCGAATGGTTCGCCTCACGCGCC
AAAGTTCCCGAAAAAATTACCGTTACCGTTTTCAAAGTTGACGGCGAAACCAATACAGACGACCTCTCCCCCGCGCCCGA
CGCGTGGAGTCGTCCCGATATTCCGCTGCACGCGCTGGCCATGCTGAAAAACCCGCGCGACGGCATCACGCCCGACAAAC
CGGGCGAAGTCGGTCCGATTAAATTGTTGGAAGAACTCAAAGCCAAAGGCCATCCGGTTGCTTACGTCGGCGACGTGGTC
GGTACTGGTTCTTCACGCAAATCCGCGACCAACTCCGTCATTTGGCATACCGGCGAAGACATTCCGTTCGTGCCGAACAA
ACGCTTCGGCGGCGTATGTTTGGGCGGCAAAATCGCGCCGATTTTCTTCAATACCCAAGAAGATTCCGGCGCGCTGCCGA
TTGAAGTCGATGTATCTGCTCTAAAAATGGGCGATGTCGTCGATATCCTGCCTTATGAAGGCAAAATCGTGAAAAACGGC
GAGACTGTTGCCGAGTTTGAATTGAAATCACAAGTATTGCTGGACGAAGTGCAAGCCGGCGGCCGTATCAACCTGATTAT
CGGTCGAGGTCTGACCGCCAAAGCGCGCGAAGCCCTGAAACTGCCTGCCTCTACTGCATTCCGCCTGCCGCAAGCGCCTG
CCGAAAGCAAAGCCGGTTTCACCTTGGCGCAAAAAATGGTCGGCCGCGCCTGCGGTCTGCCCGAAGGACAAGGCGTGCGC
CCGGGTACTTACTGCGAACCGCGTATGACGACTGTCGGCTCGCAAGACACGACCGGCCCGATGACCCGCGACGAGTTGAA
AGACTTGGCTTGTTTGGGCTTCTCCGCCGATATGGTGATGCAGTCTTTCTGCCACACCGCCGCCTATCCGAAACCTGTCG
ATGTAAAAACCCATAAAGAACTGCCCGCCTTTATTTCCACCCGTGGCGGCGTGTCACTGCGTCCGGGCGACGGCGTCATC
CACTCGTGGCTCAACCGCCTGCTGCTGCCCGATACCGTCGGCACCGGCGGCGACAGCCATACCCGTTTCCCCATCGGTAT
TTCCTTCCCCGCCGGCTCCGGCTTGGTTGCCTTTGCCGCCGCAACGGGCGTAATGCCGCTCGATATGCCCGAGTCTGTAT
TGGTACGCTTCAGCGGCAAGCTGCAACCGGGCGTAACCCTGCGCGATTTGGTGAACGCCATCCCGCTGTACGCAATCAAA
CAAGGTTTGCTGACCGTTGCCAAAGCCGGTAAGAAAAACATCTTCTCCGGCCGCATCCTCGAAATCGAAGGCCTGCCTGA
TTTGAAAGTGGAACAAGCCTTTGAATTGACCGACGCATCCGCCGAACGCTCCGCCGCCGGCTGTACCGTGAAGCTCAACA
AAGAGCCGATTATCGAGTACATGAAATCCAACGTCGTGTTGATGAAAAACATGATTGCCAACGGCTATCAAGACCCGCGC
ACTTTGGAACGCCGCATCAAAGCTATGGAAAAATGGCTGGCAAATCCCGAGTTGCTCGAAGCGGATAAAGATGCCGAATA
CGCCGCCGTGATTGAAATCAACATGGACGACATCAAAGAGCCGATTATCGCCTGCCCGAACGACCCGGACGACGTGTGCT
TCATGTCCGAACGCTCCGGCACCAAAATCGACGAAGTATTCATCGGTTCGTGTATGACCAACATCGGCCACTTCCGCGCC
GCCTCCAAACTTTTGGAAGGCAAGGCAGACACCCCCGTCCGCCTGTGGATTGCGCCGCCGACCAAAATGGACGCGAAACA
ATTGTCCGACGAAGGACACTACGGCGTACTCGGACGTGCCGGCGCGCGTATGGAAATGCCGGGTTGCTCCTTATGTATGG
GTAATCAGGCGCAAGTACGCGAAGGTGCGACCGTTATGTCCACCTCCACCCGCAACTTCCCGAACCGTTTGGGTAAAAAC
ACCTTTGTTTACCTCGGTTCGGCGGAATTGGCAGCGATTTGCTCCAAACTGGGTAAAATCCCGACCGTTGAAGAATATCA
AGCCAATATCGGCATCATCAACGAACAGGGCGATAAAATCTACCGCTATATGAACTTCAACGAAATCGACAGCTACAACG
AAGTAGCCGAGACCGTGAACGTTTAA

Upstream 100 bases:

>100_bases
GTTAAAACTAGTCTATGATAGCGGTACAAATATTGTTTACAATATTTAACGCAAATCATTTGCAACCCGACAAAAGAAAA
ACAGAAAAAGGAACAAAGAG

Downstream 100 bases:

>100_bases
TCCCCGTCATCCGTATGAAGTAAGGGATTGACCGCAATGCCGTCTGAACAACCTTCAGACGGCATTGCAACATTCCGCTA
ACCCTTCTTTCCGCAAACGC

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 861; Mature: 861

Protein sequence:

>861_residues
MLEAYRKAAAERAALGIPALPLTAQQTADLVELLKSPPAGEGEFLVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPL
ISPEYATELLGTMLGGYNIHALIELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVGPIKLLEELKAKGHPVAYVGDVV
GTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGGKIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNG
ETVAEFELKSQVLLDEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACGLPEGQGVR
PGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYPKPVDVKTHKELPAFISTRGGVSLRPGDGVI
HSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIK
QGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSNVVLMKNMIANGYQDPR
TLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPIIACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRA
ASKLLEGKADTPVRLWIAPPTKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLGKN
TFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVAETVNV

Sequences:

>Translated_861_residues
MLEAYRKAAAERAALGIPALPLTAQQTADLVELLKSPPAGEGEFLVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPL
ISPEYATELLGTMLGGYNIHALIELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVGPIKLLEELKAKGHPVAYVGDVV
GTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGGKIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNG
ETVAEFELKSQVLLDEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACGLPEGQGVR
PGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYPKPVDVKTHKELPAFISTRGGVSLRPGDGVI
HSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIK
QGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSNVVLMKNMIANGYQDPR
TLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPIIACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRA
ASKLLEGKADTPVRLWIAPPTKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLGKN
TFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVAETVNV
>Mature_861_residues
MLEAYRKAAAERAALGIPALPLTAQQTADLVELLKSPPAGEGEFLVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPL
ISPEYATELLGTMLGGYNIHALIELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVGPIKLLEELKAKGHPVAYVGDVV
GTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGGKIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNG
ETVAEFELKSQVLLDEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACGLPEGQGVR
PGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYPKPVDVKTHKELPAFISTRGGVSLRPGDGVI
HSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIK
QGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSNVVLMKNMIANGYQDPR
TLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPIIACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRA
ASKLLEGKADTPVRLWIAPPTKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLGKN
TFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVAETVNV

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=862, Percent_Identity=74.9419953596288, Blast_Score=1291, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=374, Percent_Identity=24.8663101604278, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6321429, Length=371, Percent_Identity=25.3369272237197, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 92713; Mature: 92713

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00450 ACONITASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEAYRKAAAERAALGIPALPLTAQQTADLVELLKSPPAGEGEFLVELLAHRVPPGVDDA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHH
AKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHALIELLDDDKLAPIAAKGLK
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHH
HTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRAKVPEKITVTVFKVDGETNTD
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEEEECCCCCCC
DLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVGPIKLLEELKAKGHPVAYVGDVV
CCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCC
GTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGGKIAPIFFNTQEDSGALPIEVDVSA
CCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEECCEEEEEEEECCCCCCCCEEEECHHH
LKMGDVVDILPYEGKIVKNGETVAEFELKSQVLLDEVQAGGRINLIIGRGLTAKAREALK
HCCCCEEEECCCCCEEEECCCEEEHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHC
LPASTAFRLPQAPAESKAGFTLAQKMVGRACGLPEGQGVRPGTYCEPRMTTVGSQDTTGP
CCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCC
MTRDELKDLACLGFSADMVMQSFCHTAAYPKPVDVKTHKELPAFISTRGGVSLRPGDGVI
CCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCEECCCCCHH
HSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFSGK
HHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHEECCC
LQPGVTLRDLVNAIPLYAIKQGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELTDAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCC
AERSAAGCTVKLNKEPIIEYMKSNVVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLE
CCCCCCCCEEEECCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHCC
ADKDAEYAAVIEINMDDIKEPIIACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRA
CCCCCCEEEEEEECHHHHCCCEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
ASKLLEGKADTPVRLWIAPPTKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVR
HHHHHCCCCCCCEEEEECCCCCCCHHHHCCCCCEEEEECCCCEECCCCCCEECCCHHHHH
EGATVMSTSTRNFPNRLGKNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKI
CCCEEEECCCCCHHHHHCCCEEEEECCHHHHHHHHHHCCCCCHHHHHCCCEEEECCCCEE
YRYMNFNEIDSYNEVAETVNV
EEEECCHHCCCHHHHHHHHCC
>Mature Secondary Structure
MLEAYRKAAAERAALGIPALPLTAQQTADLVELLKSPPAGEGEFLVELLAHRVPPGVDDA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHH
AKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHALIELLDDDKLAPIAAKGLK
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHH
HTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRAKVPEKITVTVFKVDGETNTD
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEEEECCCCCCC
DLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVGPIKLLEELKAKGHPVAYVGDVV
CCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCC
GTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGGKIAPIFFNTQEDSGALPIEVDVSA
CCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEECCEEEEEEEECCCCCCCCEEEECHHH
LKMGDVVDILPYEGKIVKNGETVAEFELKSQVLLDEVQAGGRINLIIGRGLTAKAREALK
HCCCCEEEECCCCCEEEECCCEEEHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHC
LPASTAFRLPQAPAESKAGFTLAQKMVGRACGLPEGQGVRPGTYCEPRMTTVGSQDTTGP
CCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCC
MTRDELKDLACLGFSADMVMQSFCHTAAYPKPVDVKTHKELPAFISTRGGVSLRPGDGVI
CCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCEECCCCCHH
HSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFSGK
HHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHEECCC
LQPGVTLRDLVNAIPLYAIKQGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELTDAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCC
AERSAAGCTVKLNKEPIIEYMKSNVVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLE
CCCCCCCCEEEECCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHCC
ADKDAEYAAVIEINMDDIKEPIIACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRA
CCCCCCEEEEEEECHHHHCCCEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
ASKLLEGKADTPVRLWIAPPTKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVR
HHHHHCCCCCCCEEEEECCCCCCCHHHHCCCCCEEEEECCCCEECCCCCCEECCCHHHHH
EGATVMSTSTRNFPNRLGKNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKI
CCCEEEECCCCCHHHHHCCCEEEEECCHHHHHHHHHHCCCCCHHHHHCCCEEEECCCCEE
YRYMNFNEIDSYNEVAETVNV
EEEECCHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]