Click here to switch to the map view.

The map label for this gene is purN [H]

Identifier: 161870421

GI number: 161870421

Start: 1495602

End: 1496324

Strand: Reverse

Name: purN [H]

Synonym: NMCC_1469

Alternate gene names: 161870421

Gene position: 1496324-1495602 (Counterclockwise)

Preceding gene: 161870422

Following gene: 161870420

Centisome position: 69.49

GC content: 53.39

Gene sequence:

>723_bases
ATGCCCGAAACAGGTTTTCGGGCATTTTTACGGCAAATGCCGTCTGAAACCACCAAACAGCGGTTCAGATGGCATCCCTT
TCAAAAGCGCACCATTATGAAAAACATCGTCATCCTGATTTCCGGACGCGGCAGCAATATGCAGGCAATCGTCAATGCCG
CCATTCCCAACGTCCGTATTGCCGCCGTGTTGAGCAACAGCGAAACGGCTGCCGGTTTGCAATGGGCGGCCGGTCAGGGT
ATTCCGACCGACAGCCTGAATCATAAAAACTTTGAATCACGACTTGCCTTCGATACCGCCATGATGGAAAAAATCGACGC
ATATCAACCCGACTTGGTGGTTTTGGCAGGTTTTATGCGGATTCTGACCCCCGAGTTTTGCGCCCATTACGAAGGCAGGC
TGATGAACATCCATCCGTCCATCCTGCCTTCATTTACTGGGCTTCATACGCACGAACGCTCTTTGGAAGCGGGTTGCCGC
GTTGCAGGCTGTACGATTCACTTTGTTACGGCCGAGCTGGATTGCGGCCCGATTGTATCGCAAGGGATTGTGCCGATACT
TGATGGCGATACGGCAGACGATATTGCCGCCCGGGTTTTGGCTGTCGAACACAAACTCTATCCGAAAGCCGTTGCCGATT
TTGCCGCCGGCCGCCTGATTATCGAGGGAAACCGCGTCAGAAATTCGGAAAACGCCGATGCCGCCCGTTTCCTGACGGCG
TAA

Upstream 100 bases:

>100_bases
GTATTTGATGTGAAACTGGTCAAAATCGGCGCACCCGAAAACGCGCCCGCCAAGCAGCCGGCTCAAGTCGACATCAAAAA
AGTAAATTAAGTCCGAATCC

Downstream 100 bases:

>100_bases
ACCGGGCGGGAGCAAATGATGAAGACTTTTAAAAATATATTTTCCGCCGCCATTTTGTCCGCCGCCCTGCCGTGCGCGTA
TGCGGCAGGGCTGCCCCAAT

Product: phosphoribosylglycinamide transformylase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MPETGFRAFLRQMPSETTKQRFRWHPFQKRTIMKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWAAGQG
IPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRLMNIHPSILPSFTGLHTHERSLEAGCR
VAGCTIHFVTAELDCGPIVSQGIVPILDGDTADDIAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA

Sequences:

>Translated_240_residues
MPETGFRAFLRQMPSETTKQRFRWHPFQKRTIMKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWAAGQG
IPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRLMNIHPSILPSFTGLHTHERSLEAGCR
VAGCTIHFVTAELDCGPIVSQGIVPILDGDTADDIAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA
>Mature_239_residues
PETGFRAFLRQMPSETTKQRFRWHPFQKRTIMKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWAAGQGI
PTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRLMNIHPSILPSFTGLHTHERSLEAGCRV
AGCTIHFVTAELDCGPIVSQGIVPILDGDTADDIAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=201, Percent_Identity=42.2885572139304, Blast_Score=166, Evalue=1e-41,
Organism=Homo sapiens, GI209869995, Length=201, Percent_Identity=42.2885572139304, Blast_Score=166, Evalue=1e-41,
Organism=Homo sapiens, GI209869993, Length=201, Percent_Identity=42.2885572139304, Blast_Score=166, Evalue=1e-41,
Organism=Escherichia coli, GI1788846, Length=193, Percent_Identity=51.8134715025907, Blast_Score=200, Evalue=8e-53,
Organism=Escherichia coli, GI1787483, Length=195, Percent_Identity=35.3846153846154, Blast_Score=119, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17567511, Length=204, Percent_Identity=37.7450980392157, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6320616, Length=201, Percent_Identity=29.3532338308458, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24582400, Length=193, Percent_Identity=40.9326424870466, Blast_Score=160, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 26342; Mature: 26210

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPETGFRAFLRQMPSETTKQRFRWHPFQKRTIMKNIVILISGRGSNMQAIVNAAIPNVRI
CCCHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHCEEEEEEECCCCCHHHHHHHCCCCEEE
AAVLSNSETAAGLQWAAGQGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMR
EEEECCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ILTPEFCAHYEGRLMNIHPSILPSFTGLHTHERSLEAGCRVAGCTIHFVTAELDCGPIVS
HHCHHHHHCCCCEEEEECHHHCCCCCCCCCHHHHHHCCCEEEEEEEEEEEEECCCCCHHH
QGIVPILDGDTADDIAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA
HCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHCCC
>Mature Secondary Structure 
PETGFRAFLRQMPSETTKQRFRWHPFQKRTIMKNIVILISGRGSNMQAIVNAAIPNVRI
CCHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHCEEEEEEECCCCCHHHHHHHCCCCEEE
AAVLSNSETAAGLQWAAGQGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMR
EEEECCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ILTPEFCAHYEGRLMNIHPSILPSFTGLHTHERSLEAGCRVAGCTIHFVTAELDCGPIVS
HHCHHHHHCCCCEEEEECHHHCCCCCCCCCHHHHHHCCCEEEEEEEEEEEEECCCCCHHH
QGIVPILDGDTADDIAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA
HCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]