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The map label for this gene is lpdA3 [H]

Identifier: 161869869

GI number: 161869869

Start: 902523

End: 903956

Strand: Direct

Name: lpdA3 [H]

Synonym: NMCC_0900

Alternate gene names: 161869869

Gene position: 902523-903956 (Clockwise)

Preceding gene: 161869868

Following gene: 161869870

Centisome position: 41.91

GC content: 52.65

Gene sequence:

>1434_bases
ATGTCTCAATATGATGTAGTAGTGATTGGTGCAGGCCCGGGCGGATACGTTGCCGCCATCCGTGCCGCACAACTGGGTTT
CAAAACTGCCTGTGTCGATGCAGGCGTTAACAAAGCAGGCAATGCCCCTGCATTGGGCGGTACTTGCTTGAACGTAGGCT
GTATCCCTTCTAAAGCCCTGTTGCAATCCAGCGAACATTTCCACGCTGCGCAACACGATTTTGCCGAACACGGTATCACT
GTCGGCGACGTAAAATTCGACGCGGCCAAAATGATTGAGCGCAAAGATGCCATCGTGACCAAACTGACCGGCGGCGTGAA
ATTCCTGTTCCAAAAAAACAAAGTAACCAGCCTGTTCGGTACTGCTTCCTTTGCCGGTAAAAATGGCGATGCTTACCAAA
TCGAAGTCGATAACAAAGGCGAGAAAACCGTTATCGAAGCCAAACACGTCATCGTAGCGACCGGTTCCGTACCGCGTCCG
CTGCCACAAGTCGCTATCGACAACGTTAACGTATTGGACAACGAAGGCGCATTGAACCTGACCGAAGTACCTGCCAAACT
CGGCGTGATCGGTTCCGGCGTGATTGGTTTGGAAATGGGTTCCGTATGGAACCGCGTGGGTTCAGAAGTTACCATTCTTG
AAGCCGCACCGACTTTCTTGGCTGCCGCCGACCAACAAATCGCCAAAGAAGCCTTCAAATACTTCACCAAAGAGCAAGGT
CTGAGCATCGAATTGGGCGTGAAAATCGGCGACATCAAATCTGAAGGCAAAGGTGTTTCCGTTGCTTACGAAACTGCTGC
CGGCGAAGCCAAAACCGAAGTATTCGACAAACTGATCGTTGCCATCGGCCGTATTCCAAACACCAAAGGCCTGAACGCGG
AAGCCGTAGGCTTGGAAAAAGACGAGCGCGGCTTTATCAAAGTAGATGGCGAATGCCGTACCAACCTGCCTAACGTATGG
GCAATCGGCGACGTGGTTCGCGGCCCGATGTTGGCACACAAAGCCAGCGACGAAGGCGTTGCCGTTGCCGAACGCATTGC
CGGTCAAAAACCGCATATCGACTTCAACAACGTACCGTTCGTGATTTACACCGATCCTGAAATCGCTTGGGTGGGTAAAA
CCGAAGAGCAGCTCAAAGCCGAAGGCGTGGAGTACAAAAAAGGTACTTCAGGTTTTGGTGCGAATGGTCGCGCATTGGCA
ATGGGCAAAGCCAAAGGTACGGTTAAAGTGTTGGCAGACGCCAAAACCGACCGCATCTTGGGCGTACACATGATTGGTCC
GGTTGTCAGCGAATTGGTTACCGAAGGCGTGACTGCGCTCGAATTCTTCGCCAGCAGCGAAGACATCGCCCGCATTATCC
ATGCCCACCCAACCTTGTCCGAAGTGGTTCACGAAGCTGCATTGGCGGCCGACAAACGCGCTTTGCACGGTTGA

Upstream 100 bases:

>100_bases
CTTTTTCTTAAAACCATCAAAACGCAGTCATTCAAAATAAAAAAGAAACGAAAAGTATCGTTTTTATTTTGAGATACTGC
TAAAAGCAAAGGATGACACG

Downstream 100 bases:

>100_bases
TAGACATTAAGGCCTCCGACAAATTGAATGTTCCGAGAGCTTCGTTTTCTGATTTATAATTCCGTCAGGCAAACAAACAG
CATTTACATTCATTATGAAC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPALGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGIT
VGDVKFDAAKMIERKDAIVTKLTGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGSEVTILEAAPTFLAAADQQIAKEAFKYFTKEQG
LSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFDKLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVW
AIGDVVRGPMLAHKASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGFGANGRALA
MGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIARIIHAHPTLSEVVHEAALAADKRALHG

Sequences:

>Translated_477_residues
MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPALGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGIT
VGDVKFDAAKMIERKDAIVTKLTGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGSEVTILEAAPTFLAAADQQIAKEAFKYFTKEQG
LSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFDKLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVW
AIGDVVRGPMLAHKASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGFGANGRALA
MGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIARIIHAHPTLSEVVHEAALAADKRALHG
>Mature_476_residues
SQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPALGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITV
GDVKFDAAKMIERKDAIVTKLTGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRPL
PQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGSEVTILEAAPTFLAAADQQIAKEAFKYFTKEQGL
SIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFDKLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWA
IGDVVRGPMLAHKASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGFGANGRALAM
GKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIARIIHAHPTLSEVVHEAALAADKRALHG

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=476, Percent_Identity=49.3697478991597, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI50301238, Length=471, Percent_Identity=29.2993630573248, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI22035672, Length=464, Percent_Identity=30.3879310344828, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI33519430, Length=469, Percent_Identity=27.5053304904051, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI33519428, Length=469, Percent_Identity=27.5053304904051, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI33519426, Length=469, Percent_Identity=27.5053304904051, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI148277065, Length=469, Percent_Identity=27.5053304904051, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI148277071, Length=469, Percent_Identity=27.5053304904051, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI291045266, Length=462, Percent_Identity=27.0562770562771, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI291045268, Length=456, Percent_Identity=24.5614035087719, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1786307, Length=462, Percent_Identity=41.7748917748918, Blast_Score=335, Evalue=6e-93,
Organism=Escherichia coli, GI87082354, Length=477, Percent_Identity=28.9308176100629, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI87081717, Length=477, Percent_Identity=29.979035639413, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1789915, Length=447, Percent_Identity=27.9642058165548, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI32565766, Length=472, Percent_Identity=48.9406779661017, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17557007, Length=475, Percent_Identity=27.5789473684211, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71982272, Length=484, Percent_Identity=26.8595041322314, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=469, Percent_Identity=27.5053304904051, Blast_Score=118, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI71983429, Length=469, Percent_Identity=27.5053304904051, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17559934, Length=227, Percent_Identity=30.3964757709251, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=484, Percent_Identity=46.900826446281, Blast_Score=399, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6325240, Length=479, Percent_Identity=34.446764091858, Blast_Score=242, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6325166, Length=475, Percent_Identity=30.3157894736842, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI21358499, Length=472, Percent_Identity=48.9406779661017, Blast_Score=422, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24640549, Length=492, Percent_Identity=28.8617886178862, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24640553, Length=492, Percent_Identity=28.8617886178862, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24640551, Length=492, Percent_Identity=28.8617886178862, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI17737741, Length=476, Percent_Identity=27.9411764705882, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50096; Mature: 49965

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPALGGTCLNVGCIPSKAL
CCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHH
LQSSEHFHAAQHDFAEHGITVGDVKFDAAKMIERKDAIVTKLTGGVKFLFQKNKVTSLFG
HCCCCHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEE
TASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRPLPQVAIDNVNVLDNEGALNL
CCEECCCCCCEEEEEECCCCCEEEEEEEEEEEECCCCCCCCCHHHCCCCEEECCCCCCCH
TEVPAKLGVIGSGVIGLEMGSVWNRVGSEVTILEAAPTFLAAADQQIAKEAFKYFTKEQG
HHCCHHHCEEECCEEEEEHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
LSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFDKLIVAIGRIPNTKGLNAEAVGLEK
CEEEEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEECCCC
DERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHKASDEGVAVAERIAGQKPHIDFNNVPF
CCCCEEEECCCCCCCCCCEEEEHHHHCCCHHEECCCCCCHHHHHHHCCCCCCCCCCCCCE
VIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGFGANGRALAMGKAKGTVKVLADAKTDRIL
EEEECCCEEEECCCHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCEE
GVHMIGPVVSELVTEGVTALEFFASSEDIARIIHAHPTLSEVVHEAALAADKRALHG
EEHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPALGGTCLNVGCIPSKAL
CCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHH
LQSSEHFHAAQHDFAEHGITVGDVKFDAAKMIERKDAIVTKLTGGVKFLFQKNKVTSLFG
HCCCCHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEE
TASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRPLPQVAIDNVNVLDNEGALNL
CCEECCCCCCEEEEEECCCCCEEEEEEEEEEEECCCCCCCCCHHHCCCCEEECCCCCCCH
TEVPAKLGVIGSGVIGLEMGSVWNRVGSEVTILEAAPTFLAAADQQIAKEAFKYFTKEQG
HHCCHHHCEEECCEEEEEHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
LSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFDKLIVAIGRIPNTKGLNAEAVGLEK
CEEEEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEECCCC
DERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHKASDEGVAVAERIAGQKPHIDFNNVPF
CCCCEEEECCCCCCCCCCEEEEHHHHCCCHHEECCCCCCHHHHHHHCCCCCCCCCCCCCE
VIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGFGANGRALAMGKAKGTVKVLADAKTDRIL
EEEECCCEEEECCCHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCEE
GVHMIGPVVSELVTEGVTALEFFASSEDIARIIHAHPTLSEVVHEAALAADKRALHG
EEHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]