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The map label for this gene is sucB [H]

Identifier: 161869868

GI number: 161869868

Start: 900947

End: 902158

Strand: Direct

Name: sucB [H]

Synonym: NMCC_0899

Alternate gene names: 161869868

Gene position: 900947-902158 (Clockwise)

Preceding gene: 161869867

Following gene: 161869869

Centisome position: 41.84

GC content: 52.72

Gene sequence:

>1212_bases
ATGATTATTGATGTAAAAGTACCTATGCTGTCTGAAAGCGTATCTGAAGGCACGCTCTTGGAATGGAAGAAAAAAGTTGG
CGAAGCAGTTGCCCGTGACGAAATCCTGATCGATATCGAAACGGACAAAGTGGTTTTGGAAGTACCTTCTCCACAAGCCG
GCGTATTGGTTGAAATCGTAGCGCAAGACGGTGAAACCGTTGTTGCCGACCAAGTTTTGGCACGTATCGATACCGCTGCT
ACTGCCGCTGCTGAAGCACCTGCCGCTGCTACTGCCGCTGCTGAAGCACCTGCCGCCGCTCCTGCCGAAGCTGCCCCGGC
CGCCGCTCCTGCTGCTGCACAAAACAACGCCGCTATGCCTGCCGCCGCCAAACTGGCTGCAGAGAGCGGTGTTGACGTGA
ACGCATTGCAAGGTTCCGGCCGTGACGGTCGCGTATTGAAAGAAGACGTACAAAATGCCGCTGCCAAACCTGCCGCAGCC
GCTGCTCCTGCTGTTGCACTTCCTGCCGGCGCACGTCCTGAAGAACGCGTACCAATGAGCCGCCTGCGTGCCCGTGTTGC
AGAACGCCTCTTGGCTTCTCAACAAGAAAACGCCATTCTGACTACATTCAACGAAGTCAACATGAAACCAATCATGGACT
TGCGTGCGAAGTACAAAGAAAAATTCGAGAAAGAACACGGCGTGAAACTGGGCTTTATGTCCTTCTTCGTTAAAGCCGCT
GTTGCCGCCCTGAAAAAATACCCGGTTGTGAATGCTTCTGTTGACGGCAAAGACATCGTGTACCACGGCTACTTCGACAT
CGGTATCGCAATTGGCAGCCCACGCGGTTTGGTTGTGCCAATCCTGCGCGATGCCGACCAAATGAGCATTGCCGACATCG
AACAAGCAATTGTTGATTACGCGAAAAAAGCCAAAGACGGCAAAATCGCTATCGAAGATCTGACCGGCGGTACATTCAGT
ATTACCAACGGCGGTACTTTCGGTTCTATGATGTCCACCCCGATCATCAATCCGCCTCAATCTGCGATTTTGGGTATGCA
CGCCACTAAAGAGCGCGCTGTGGTTGAAAACGGCCAAGTTGTTGTCCGTCCAATGATGTATCTGGCTCTGTCTTACGACC
ACCGTATCATTGACGGCCGCGAAGCTGTATTGACCTTGGTAGCCATTAAAGACGCGTTGGAAGACCCAGCCCGCCTGTTG
TTGGATCTGTAA

Upstream 100 bases:

>100_bases
AGCCAAGTAGCATTCCGTCTGAGGCTGCTCAGGCGGAACACCCATATGCAGAATGAAAAACACACAACAGGCCGTCTGAA
AGGGCCATTGGAGACACAAA

Downstream 100 bases:

>100_bases
TCGTTTCAGACGGCCTTTTATTTGTTAATGAAAAGGCCGTCTGAATTTTTATAGTGGATTAAATTTAAACCAGTACGGCG
TTGCCTCGCCTTGCCGTACT

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKVVLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAA
TAAAEAPAAATAAAEAPAAAPAEAAPAAAPAAAQNNAAMPAAAKLAAESGVDVNALQGSGRDGRVLKEDVQNAAAKPAAA
AAPAVALPAGARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGVKLGFMSFFVKAA
VAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDADQMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFS
ITNGGTFGSMMSTPIINPPQSAILGMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLL
LDL

Sequences:

>Translated_403_residues
MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKVVLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAA
TAAAEAPAAATAAAEAPAAAPAEAAPAAAPAAAQNNAAMPAAAKLAAESGVDVNALQGSGRDGRVLKEDVQNAAAKPAAA
AAPAVALPAGARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGVKLGFMSFFVKAA
VAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDADQMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFS
ITNGGTFGSMMSTPIINPPQSAILGMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLL
LDL
>Mature_403_residues
MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKVVLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAA
TAAAEAPAAATAAAEAPAAAPAEAAPAAAPAAAQNNAAMPAAAKLAAESGVDVNALQGSGRDGRVLKEDVQNAAAKPAAA
AAPAVALPAGARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGVKLGFMSFFVKAA
VAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDADQMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFS
ITNGGTFGSMMSTPIINPPQSAILGMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLL
LDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=256, Percent_Identity=55.078125, Blast_Score=288, Evalue=9e-78,
Organism=Homo sapiens, GI31711992, Length=433, Percent_Identity=29.3302540415704, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI110671329, Length=428, Percent_Identity=28.0373831775701, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI203098816, Length=458, Percent_Identity=26.8558951965066, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI203098753, Length=456, Percent_Identity=26.0964912280702, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI260898739, Length=166, Percent_Identity=34.9397590361446, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1786946, Length=408, Percent_Identity=55.6372549019608, Blast_Score=454, Evalue=1e-129,
Organism=Escherichia coli, GI1786305, Length=406, Percent_Identity=34.2364532019704, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI25146366, Length=406, Percent_Identity=44.0886699507389, Blast_Score=331, Evalue=5e-91,
Organism=Caenorhabditis elegans, GI17560088, Length=431, Percent_Identity=30.1624129930394, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17537937, Length=426, Percent_Identity=28.4037558685446, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=315, Percent_Identity=30.4761904761905, Blast_Score=127, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6320352, Length=400, Percent_Identity=43.25, Blast_Score=332, Evalue=6e-92,
Organism=Saccharomyces cerevisiae, GI6324258, Length=450, Percent_Identity=27.7777777777778, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24645909, Length=228, Percent_Identity=57.0175438596491, Blast_Score=278, Evalue=4e-75,
Organism=Drosophila melanogaster, GI18859875, Length=423, Percent_Identity=29.0780141843972, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI20129315, Length=233, Percent_Identity=32.1888412017167, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24582497, Length=233, Percent_Identity=32.1888412017167, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 42326; Mature: 42326

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKVVLEVPSPQAGVLVEIV
CEEEEECCHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCEEEEECCCCCCCEEEEEE
AQDGETVVADQVLARIDTAATAAAEAPAAATAAAEAPAAAPAEAAPAAAPAAAQNNAAMP
ECCCCEEHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
AAAKLAAESGVDVNALQGSGRDGRVLKEDVQNAAAKPAAAAAPAVALPAGARPEERVPMS
HHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHCCCCHHCCCCEECCCCCCCCCCCCHH
RLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGVKLGFMSFFVKAA
HHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHH
VAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDADQMSIADIEQAIVDY
HHHHHHCCCEECCCCCCEEEEEEEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHH
AKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAILGMHATKERAVVENGQV
HHHCCCCCEEEEECCCCEEEECCCCCCHHHHCCCCCCCCHHHHCCCCCCCCHHHHCCCCE
VVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLLLDL
EEHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCHHHHHHCC
>Mature Secondary Structure
MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKVVLEVPSPQAGVLVEIV
CEEEEECCHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCEEEEECCCCCCCEEEEEE
AQDGETVVADQVLARIDTAATAAAEAPAAATAAAEAPAAAPAEAAPAAAPAAAQNNAAMP
ECCCCEEHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
AAAKLAAESGVDVNALQGSGRDGRVLKEDVQNAAAKPAAAAAPAVALPAGARPEERVPMS
HHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHCCCCHHCCCCEECCCCCCCCCCCCHH
RLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGVKLGFMSFFVKAA
HHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHH
VAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDADQMSIADIEQAIVDY
HHHHHHCCCEECCCCCCEEEEEEEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHH
AKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAILGMHATKERAVVENGQV
HHHCCCCCEEEEECCCCEEEECCCCCCHHHHCCCCCCCCHHHHCCCCCCCCHHHHCCCCE
VVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLLLDL
EEHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]