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The map label for this gene is dnaB [H]

Identifier: 161869818

GI number: 161869818

Start: 851164

End: 852468

Strand: Direct

Name: dnaB [H]

Synonym: NMCC_0847

Alternate gene names: 161869818

Gene position: 851164-852468 (Clockwise)

Preceding gene: 161869816

Following gene: 161869819

Centisome position: 39.53

GC content: 54.94

Gene sequence:

>1305_bases
ATGCTGGAAAATCCGGCATGGGACAGGATTGCCGATGTGGTTTCGGGAGAGGATTTCTACCGGCATGAACACCGCCTGAT
TTTCCGTTCCATTGCCAAATTAATCAATGAGAGCCGTCCCGCCGATGTGATTACGGTTCAGGAAGATTTGCAGCGGAACG
AAGAATTGGAAGCGGCAGGCGGATTTGAATATCTGATTACGCTGGCGCAAAATACTCCGTCTGCCGCCAACATCCGCCGC
TACGCCGAAATCGTGCGCGAGCGTTCCATCATGCGCCAACTCGCCGAAGTGGGGACGGAAATCGCCCGCAGCGCCTACAA
TCCGCAAGGCAGGGACGCGGGGCAGCTTTTAGATGAAGCGGAAAACAAAGTATTCCAAATCGCCGAAAGCACCGCCAAAT
CCAAGCAGGGCTTTTTGGAGATGCCCGATTTGCTGAAAGAAGTCGTACAGCGCATCGATATGCTCTACTCGCGCGACAAT
CCCGATGAAGTTACCGGCGTGCCGACGGGGTTCATCGACCTCGACAAAAAAACCTCGGGCCTTCAGCCCGGCGACCTGAT
TATCGTTGCCGGTCGTCCGTCTATGGGTAAGACCGCCTTTTCCATCAATATCGCCGAACACGTTGCCGTAGAAGGCAAGC
TGCCGGTTGCCGTCTTCTCAATGGAGATGGGCGGGGCGCAACTGGTCATGCGTATGCTCGGCTCGGTCGGACGCTTGGAT
CAAAGCGTTTTGAAAACCGGCAGGCTCGAAGACGAACACTGGGGTCGCCTGAACGAAGCAGTCGTCAAACTCTCCGACGC
GCCCGTGTACATCGACGAGACCCCGGGTTTGACTGCGCTCGAACTGCGCGCCCGTGCCCGCCGTCTCGCCCGTCAATTTA
ACAATAAGCTTGGATTAATCGTCATCGACTACCTGCAACTGATGGCAGGATCCGGCCGTTCCGACAACCGAGCTTCGGAG
CTGGGAGAGATTTCACGTTCGCTCAAAGCGTTGGCGAAAGAATTGCAAGTCCCCATCATCGCCCTGTCGCAATTGAGCCG
TACCGTCGAATCGCGTACCGACAAACGCCCGATGATGTCCGACCTCCGCGAATCCGGTGCTATCGAACAAGATGCCGACC
TGATTATGTTCATGTACCGCGACGAATACTACAACCAAGACTCGCCCATGAAAGGCCTTGCCGAATGTATCATCGGCAAA
CACCGCAACGGTCCCGTCGGTAAAATCTTCCTCACATGGACGGGACAATTCACCAAATTCGACAATGCTGCCTATATTCC
CGAGGAGGCAAAGATAGAAGATTAA

Upstream 100 bases:

>100_bases
GAACGATTACACAGCCATGCTGTCTGAAGACGGAGGAATCGGCGCATTGTCGCTTCCGCCGCACTCAATGGAGGCGGAGC
AATCGGTTTTGGGCGGGTTG

Downstream 100 bases:

>100_bases
ATGGCTATATAAAAATTTATTAGGCGAAATCAGGCAAAATCGTTTAAAATCATGCTGAGAGATTGCCCTAAAAAATAAAA
CGCGGTCTTGAGGCATTTTT

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MLENPAWDRIADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITLAQNTPSAANIRR
YAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQIAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDN
PDEVTGVPTGFIDLDKKTSGLQPGDLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLD
QSVLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLIVIDYLQLMAGSGRSDNRASE
LGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPMMSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGK
HRNGPVGKIFLTWTGQFTKFDNAAYIPEEAKIED

Sequences:

>Translated_434_residues
MLENPAWDRIADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITLAQNTPSAANIRR
YAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQIAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDN
PDEVTGVPTGFIDLDKKTSGLQPGDLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLD
QSVLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLIVIDYLQLMAGSGRSDNRASE
LGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPMMSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGK
HRNGPVGKIFLTWTGQFTKFDNAAYIPEEAKIED
>Mature_434_residues
MLENPAWDRIADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITLAQNTPSAANIRR
YAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQIAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDN
PDEVTGVPTGFIDLDKKTSGLQPGDLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLD
QSVLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLIVIDYLQLMAGSGRSDNRASE
LGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPMMSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGK
HRNGPVGKIFLTWTGQFTKFDNAAYIPEEAKIED

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=425, Percent_Identity=53.6470588235294, Blast_Score=458, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 48476; Mature: 48476

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLENPAWDRIADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAG
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHCCCHHHHCC
GFEYLITLAQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEA
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ENKVFQIAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSG
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCC
LQPGDLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLD
CCCCCEEEEECCCCCCCEEEEEEHHHHHEECCCCCEEEEEECCCHHHHHHHHHHHHHHHH
QSVLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
HHHHHHCCCCHHHHHHHHHHHHEECCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHH
VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPMMS
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHH
DLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQFTKF
HHHHCCCCCCCCCEEEEEEHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCEEEC
DNAAYIPEEAKIED
CCCEECCCCCCCCC
>Mature Secondary Structure
MLENPAWDRIADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAG
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHCCCHHHHCC
GFEYLITLAQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEA
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ENKVFQIAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSG
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCC
LQPGDLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLD
CCCCCEEEEECCCCCCCEEEEEEHHHHHEECCCCCEEEEEECCCHHHHHHHHHHHHHHHH
QSVLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
HHHHHHCCCCHHHHHHHHHHHHEECCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHH
VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPMMS
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHH
DLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQFTKF
HHHHCCCCCCCCCEEEEEEHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCEEEC
DNAAYIPEEAKIED
CCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]