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The map label for this gene is rfaD [H]

Identifier: 161869769

GI number: 161869769

Start: 796461

End: 797465

Strand: Direct

Name: rfaD [H]

Synonym: NMCC_0789

Alternate gene names: 161869769

Gene position: 796461-797465 (Clockwise)

Preceding gene: 161869768

Following gene: 161869770

Centisome position: 36.99

GC content: 51.24

Gene sequence:

>1005_bases
ATGACCATCATCGTAACAGGCGCGGCCGGCTTTATCGGCAGCAACATCGTCAAAGCACTTAATCAACGCGGTATTACTGA
CATTGTTGCCGTCGATAATTTGAGCAAAGGCGAAAAATTCAAAAACCTTGCCGAGTGCGAAATCGCCCATTATCTCGACA
AACATGAATTCATCCGCCAAGTGAGGGAACACATTTTACCTTATCAAAACATCGAAGCCGTTTTCCATCAAGGCGCGTGT
TCCGATACGATGAACCACGACGGTTTGTATATGATGGACAACAACTACCAGTACACGCTGGATCTGTTGGACTGGTGTCA
GGACGAACGCATTCCCTTCCTTTATGCCTCCAGTGCGGCGGTTTACGGCAAAGGCGAAATCTTCCGCGAAGAGCGCGAAC
TCGAAAAACCGCTCAACGTGTACGGCTACTCCAAATTCCTGTTTGACCAAGTATTGCGCCGCCGCATGAAAGAAGGTCTC
ACCGCCCAAGTCGTCGGCTTCCGCTACTTCAACGTTTACGGACAACACGAACAACACAAAGGTCGCATGGCATCCGTCGC
CTTCCACCACTTCCACCAATACCGCGAACACGGTTACGTCAACCTGTTCGGCAGCAACGACGGCTACGGCAACGGCGAAC
AAACCCGCGACTTCGTCAGCGTCGAAGACGTTGCCAAAGTCAACCTCTACTTCTTCGACCATCCCGAACTTTCCGGCATC
TACAACCTCGGAACAGGCCGCAGCCAACAGTTCAACGAACTCGCTGCCGCCACCGTCAACGCCTGCCGCGCCGCCGAAGG
CAAACCCGAAATGAGCTTGAAAGAGCTGGTAGAAGAAGAACTTATCCGCTACATCCCATTTCCCGATGCGCTCAAAGGCA
AATACCAAAGCTTCACTCAAGCCGACATCACCAAATTGCGTGAGGCAGGTTATACGGAAGAATTTTTCGATGTCAAATCA
GGCGTCGAGCGCTACGTCAAATGGATGCTGGAAAATTTGGCTTAA

Upstream 100 bases:

>100_bases
AAATATTTGGAATATCATCGCAAATTTGTGTTTCAGGACAATATCGATGATTTCTTGTAACTAAGTTTATTAATTTTACT
CAAAACAAAGGAAACCGAAT

Downstream 100 bases:

>100_bases
TTTGAATGCCCGTAAAAAATCGTCTGAAAATATCAGGCGATTTCGATTTGTTTAACTTTTATATGGATTTCGATGATGAC
CGAAATACAACAACGCGCCC

Product: ADP-L-glycero-D-mannoheptose epimerase

Products: NA

Alternate protein names: ADP-L-glycero-beta-D-manno-heptose-6-epimerase; ADP-glyceromanno-heptose 6-epimerase; ADP-hep 6-epimerase; AGME [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLAECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGAC
SDTMNHDGLYMMDNNYQYTLDLLDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDFVSVEDVAKVNLYFFDHPELSGI
YNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKELVEEELIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKS
GVERYVKWMLENLA

Sequences:

>Translated_334_residues
MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLAECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGAC
SDTMNHDGLYMMDNNYQYTLDLLDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDFVSVEDVAKVNLYFFDHPELSGI
YNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKELVEEELIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKS
GVERYVKWMLENLA
>Mature_333_residues
TIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLAECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACS
DTMNHDGLYMMDNNYQYTLDLLDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGLT
AQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDFVSVEDVAKVNLYFFDHPELSGIY
NLGTGRSQQFNELAAATVNACRAAEGKPEMSLKELVEEELIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKSG
VERYVKWMLENLA

Specific function: Catalyzes the interconversion between ADP-D-glycero- beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. HldD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790049, Length=328, Percent_Identity=53.0487804878049, Blast_Score=324, Evalue=5e-90,
Organism=Saccharomyces cerevisiae, GI6319493, Length=301, Percent_Identity=27.5747508305648, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR011912
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.20 [H]

Molecular weight: Translated: 38424; Mature: 38293

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLAECEIAHYLDKHEFIRQ
CEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VREHILPYQNIEAVFHQGACSDTMNHDGLYMMDNNYQYTLDLLDWCQDERIPFLYASSAA
HHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEEHHHHHCCCCCCCEEEECCEE
VYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGLTAQVVGFRYFNVYGQHEQHK
EECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHEEEECCCCHHHC
GRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDFVSVEDVAKVNLYFFDHPELSGI
CHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHEEEEEEECCCCCCCE
YNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKELVEEELIRYIPFPDALKGKYQSFTQ
EECCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCHHHHHHH
ADITKLREAGYTEEFFDVKSGVERYVKWMLENLA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLAECEIAHYLDKHEFIRQ
EEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VREHILPYQNIEAVFHQGACSDTMNHDGLYMMDNNYQYTLDLLDWCQDERIPFLYASSAA
HHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEEHHHHHCCCCCCCEEEECCEE
VYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGLTAQVVGFRYFNVYGQHEQHK
EECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHEEEECCCCHHHC
GRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDFVSVEDVAKVNLYFFDHPELSGI
CHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHEEEEEEECCCCCCCE
YNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKELVEEELIRYIPFPDALKGKYQSFTQ
EECCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCHHHHHHH
ADITKLREAGYTEEFFDVKSGVERYVKWMLENLA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA