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The map label for this gene is nmgII [H]

Identifier: 161869767

GI number: 161869767

Start: 794065

End: 795300

Strand: Direct

Name: nmgII [H]

Synonym: NMCC_0787

Alternate gene names: 161869767

Gene position: 794065-795300 (Clockwise)

Preceding gene: 161869766

Following gene: 161869768

Centisome position: 36.87

GC content: 33.66

Gene sequence:

>1236_bases
ATGACTATTTCGACAATGGCACAGACACACGATACTCGATTACAAAAAACTTTGCTCTTTCCCAGTCAACAAGGTTGGAA
ATCTAAAACTTTTCTTAAACCTGATTCACATATTAGATTAGCAACCGTATTCAGCGGGATTGGTGCGGTTGAACAGGCAT
TCCACCGATTAAATTTAAACCATACCATTGTTTTTGCAGGAGATATTGATCCATACGTTAAAAAAAGTTATCTTGGAAAC
TATAAATTAAATGAAGATTTTTGGCATAACGACATTACTCAATTTGATGCGAGCAAGTTTAGAAATCAAGTTGATATTTT
AGTTGGAGGCAGTCCTTGCCAAGCATTTTCCATGGTTGGCAAACGTGCAGGATTAGAAGATACACGAGGAACATTATTCT
ATGAATTTGCTCGCGTTGTGGATGAAGTCCAACCAAAGATATTTATTTATGAAAATGTAAAGGGCTTGCTCAATCATGAT
AATGGAAAAACTTGGAAAGTTGTAAAAAGTGTTTTTTATTCACTTGGTTATGACTTATATTTCCAAATAATGAATAGTAA
GGATTATGGGATTCCTCAACATCGTGAGCGTATTTTTGTTGTTGGCTTTCATACCCCTCCTATAAATGGTTTTCAGTTTC
CTGAAAAGATTGAATTAGAACATACTATGCAAGATTTTTTAGAGGACTATACTGATAGCAAATATTTTTTACGTGAAAAG
GGTGTGAAATTTGTTACCAGTTCTAAAAATAGACAAAAACGTTATACACAGATTAATGGAGAAATTGCCTTATGCCAAAA
AGCAAATCAACAATTTAATTGGCATGGTGATTTTATTTTTCAGGCAGCCCGCGAATCTGAATTTGATGACTTTATTTTTG
ATGTAAATAACGTTGAGGAAAAATATTATCTTTCTGAAAAAATCAAAAATTATGTTTTGGCAGGAGGAACAAAAAATTTT
AAAACCAGTACGGAAACTGATTTGCCTGTAGCTCGCCCATTATTGCAAACTATGCATAAAATGCATCGTGCCGGGGTTGA
TAATTATGTTACTCATAATCGTGGACGTATTCGTAAATTAACACCTAGGGAATGTTTGCGGCTAATGGGTTTTAGAGATG
ATTTTAAAATTTTAGTTAGTGATACTCAAATGTATCGCCAAGCGGGAAACAGTATTGTTGTTGATGTATTAATCGCTATA
TTAAAACAAATGGATATTACGCTTTATGGAGAGTAA

Upstream 100 bases:

>100_bases
CGGTACGGCGGTTTGCTCGTTTGCCGAATTGACCAAGGCATTGTCAGGGCAATCAACAATGTAGTTTTCATATTGATAAG
ATAAACAGAACGATATAAGT

Downstream 100 bases:

>100_bases
AATGCCGGATTTTCAAAGAATTACTATTGAAAACATAGAGTATTTTATTGTCGATAGTATTCAAAATTTACGTGCGGAAG
ATAGTTTTATTCACAGGAAT

Product: DNA (cytosine-5-)-methyltransferase

Products: NA

Alternate protein names: M.HpaII; Cytosine-specific methyltransferase HpaII [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVFSGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGN
YKLNEDFWHNDITQFDASKFRNQVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIELEHTMQDFLEDYTDSKYFLREK
GVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHGDFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNF
KTSTETDLPVARPLLQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSIVVDVLIAI
LKQMDITLYGE

Sequences:

>Translated_411_residues
MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVFSGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGN
YKLNEDFWHNDITQFDASKFRNQVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIELEHTMQDFLEDYTDSKYFLREK
GVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHGDFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNF
KTSTETDLPVARPLLQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSIVVDVLIAI
LKQMDITLYGE
>Mature_410_residues
TISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVFSGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNY
KLNEDFWHNDITQFDASKFRNQVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHDN
GKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIELEHTMQDFLEDYTDSKYFLREKG
VKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHGDFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFK
TSTETDLPVARPLLQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSIVVDVLIAIL
KQMDITLYGE

Specific function: This methylase recognizes the double-stranded sequence CCGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the HpaII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=212, Percent_Identity=32.0754716981132, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 47730; Mature: 47599

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVFSGIGAVEQAFHRLNLN
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCC
HTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDASKFRNQVDILVGGSPCQAFSMVG
EEEEEECCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCCHHHHHHHH
KRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHDNGKTWKVVKSVFYSLGYDLY
HHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCHHHHHHHHHHHHCHHHE
FQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIELEHTMQDFLEDYTDSKYFLREK
EEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHC
GVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHGDFIFQAARESEFDDFIFDVNNVEE
CCEEEECCCCHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCHHEEECCCCHH
KYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPLLQTMHKMHRAGVDNYVTHNRGRIRKL
HHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCCCCCEEEC
TPRECLRLMGFRDDFKILVSDTQMYRQAGNSIVVDVLIAILKQMDITLYGE
CHHHHHHHCCCCCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHCCEEEECC
>Mature Secondary Structure 
TISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVFSGIGAVEQAFHRLNLN
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCC
HTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDASKFRNQVDILVGGSPCQAFSMVG
EEEEEECCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCCHHHHHHHH
KRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHDNGKTWKVVKSVFYSLGYDLY
HHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCHHHHHHHHHHHHCHHHE
FQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIELEHTMQDFLEDYTDSKYFLREK
EEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHC
GVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHGDFIFQAARESEFDDFIFDVNNVEE
CCEEEECCCCHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCHHEEECCCCHH
KYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPLLQTMHKMHRAGVDNYVTHNRGRIRKL
HHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCCCCCEEEC
TPRECLRLMGFRDDFKILVSDTQMYRQAGNSIVVDVLIAILKQMDITLYGE
CHHHHHHHCCCCCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2183189; 7514149 [H]