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The map label for this gene is rfaE [H]

Identifier: 161869766

GI number: 161869766

Start: 793057

End: 794028

Strand: Direct

Name: rfaE [H]

Synonym: NMCC_0786

Alternate gene names: 161869766

Gene position: 793057-794028 (Clockwise)

Preceding gene: 161869765

Following gene: 161869767

Centisome position: 36.83

GC content: 55.97

Gene sequence:

>972_bases
ATGCCCGCCAAGTTTCAACAAGAAACCCTCAAATCCCGTTTCGCACAAGCCAAAGTCCTGGTTGTTGGCGACGTGATGCT
CGACCGCTATTGGTTTGGCGATGTGTCCCGTATTTCGCCCGAAGCCCCTGTTCCCGTAGCAAAAATCGGACGTATCGACC
AACGCGCGGGCGGTGCGGCGAACGTTGCGCGCAACATCGCTTCGTTGGGCGGCAGGGCAGGGCTGTTGTCGGTAACCGGC
GACGACGAAGCCGCCGACGCGCTCGACGCGCTGATGGTACAAGACGGCGTCGCCTCCTATCTGATGCGCGACAAGCAAAT
CGCCACCACCGTCAAACTGCGCGTCGTCGCCCGCAACCAGCAGCTTATCCGCCTTGATTTTGAAGAACATCCCAACCGCG
AAGTATTGGAACAAATCAAGCAGAAATACCTCGAAGTATTGTCCGAATACGATGCAATTATTTTTTCAGACTACGGCAAA
GGCGGCCTGTCGCACATCTCCGATATGATCGATTGTGCGAAACACGAAGGCAAAATCGTATTAATCGACCCCAAAGGCGA
TGATTACGAAAAATACGCCGGCGCAACCCTGATTACGCCCAACCGCGCCGAATTGAAAGAAGTGGTCGGCAGTTGGAAAA
ACGAAGGCGAGCTGACCGAAAAAGCGCAAAACCTGCGCCGCCACCTCGACCTGACCGCCGTTTTACTGACCCGAAGCGAA
GAAGGCATGACCTTGTTCAGTGAAGGCGAACCGATTTACCAGCCCACCCGCGCCCAAGAAGTTTACGACGTATCCGGTGC
GGGCGACACCGTCATTGCCGGAATGGGCTTGGGTTTGGCGGCAGGCTGCACCATGCCCGAAGCCATGTACCTTGCCAATA
CTGCGGCCGGGGTTGTCGTGGCGAAACTCGGTACGGCGGTTTGCTCGTTTGCCGAATTGACCAAGGCATTGTCAGGGCAA
TCAACAATGTAG

Upstream 100 bases:

>100_bases
GTATTGCGCGAAGTGAACCGCGTGGCAAACCTTGAAGCAAACTGATTTTCAGGCGGCCTTACCGGTTAAGGTCGTCTGAA
CAAAACACAACGGAGGCAAT

Downstream 100 bases:

>100_bases
TTTTCATATTGATAAGATAAACAGAACGATATAAGTATGACTATTTCGACAATGGCACAGACACACGATACTCGATTACA
AAAAACTTTGCTCTTTCCCA

Product: ADP-heptose synthetase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MPAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPVPVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTG
DDEAADALDALMVQDGVASYLMRDKQIATTVKLRVVARNQQLIRLDFEEHPNREVLEQIKQKYLEVLSEYDAIIFSDYGK
GGLSHISDMIDCAKHEGKIVLIDPKGDDYEKYAGATLITPNRAELKEVVGSWKNEGELTEKAQNLRRHLDLTAVLLTRSE
EGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMGLGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELTKALSGQ
STM

Sequences:

>Translated_323_residues
MPAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPVPVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTG
DDEAADALDALMVQDGVASYLMRDKQIATTVKLRVVARNQQLIRLDFEEHPNREVLEQIKQKYLEVLSEYDAIIFSDYGK
GGLSHISDMIDCAKHEGKIVLIDPKGDDYEKYAGATLITPNRAELKEVVGSWKNEGELTEKAQNLRRHLDLTAVLLTRSE
EGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMGLGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELTKALSGQ
STM
>Mature_322_residues
PAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPVPVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGD
DEAADALDALMVQDGVASYLMRDKQIATTVKLRVVARNQQLIRLDFEEHPNREVLEQIKQKYLEVLSEYDAIIFSDYGKG
GLSHISDMIDCAKHEGKIVLIDPKGDDYEKYAGATLITPNRAELKEVVGSWKNEGELTEKAQNLRRHLDLTAVLLTRSEE
GMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMGLGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELTKALSGQS
TM

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=309, Percent_Identity=51.4563106796116, Blast_Score=300, Evalue=6e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.-

Molecular weight: Translated: 34912; Mature: 34781

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPVPVAKIGRIDQRAGGAA
CCCCHHHHHHHHHHHHCEEEEEHHHHHHHHHCCCHHHCCCCCCCCHHHHCCHHHHCCCHH
NVARNIASLGGRAGLLSVTGDDEAADALDALMVQDGVASYLMRDKQIATTVKLRVVARNQ
HHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCC
QLIRLDFEEHPNREVLEQIKQKYLEVLSEYDAIIFSDYGKGGLSHISDMIDCAKHEGKIV
EEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHCCCEEE
LIDPKGDDYEKYAGATLITPNRAELKEVVGSWKNEGELTEKAQNLRRHLDLTAVLLTRSE
EECCCCCCHHHHCCCEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEECCC
EGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMGLGLAAGCTMPEAMYLANTAAGVVV
CCCEEEECCCCCCCCCCCCCEEECCCCCCEEEEECCCCHHCCCCCCHHHHHHHHHHHHHH
AKLGTAVCSFAELTKALSGQSTM
HHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPVPVAKIGRIDQRAGGAA
CCCHHHHHHHHHHHHCEEEEEHHHHHHHHHCCCHHHCCCCCCCCHHHHCCHHHHCCCHH
NVARNIASLGGRAGLLSVTGDDEAADALDALMVQDGVASYLMRDKQIATTVKLRVVARNQ
HHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCC
QLIRLDFEEHPNREVLEQIKQKYLEVLSEYDAIIFSDYGKGGLSHISDMIDCAKHEGKIV
EEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHCCCEEE
LIDPKGDDYEKYAGATLITPNRAELKEVVGSWKNEGELTEKAQNLRRHLDLTAVLLTRSE
EECCCCCCHHHHCCCEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEECCC
EGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMGLGLAAGCTMPEAMYLANTAAGVVV
CCCEEEECCCCCCCCCCCCCEEECCCCCCEEEEECCCCHHCCCCCCHHHHHHHHHHHHHH
AKLGTAVCSFAELTKALSGQSTM
HHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA