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The map label for this gene is cysK

Identifier: 161869707

GI number: 161869707

Start: 731104

End: 732036

Strand: Direct

Name: cysK

Synonym: NMCC_0727

Alternate gene names: 161869707

Gene position: 731104-732036 (Clockwise)

Preceding gene: 161869706

Following gene: 161869708

Centisome position: 33.95

GC content: 53.27

Gene sequence:

>933_bases
ATGAAAATTGCAAACAGCATCACCGAACTAATCGGCAACACGCCTTTGGTCAAACTGAACCGTCTGACCGAAGGTTTGAA
GGCGGAGGTTGCCGTGAAACTGGAATTTTTCAATCCAGGCAGCAGCGTCAAAGACCGCATTGCCGAAGCAATGATCGAGG
GTGCCGAAAAAGCGGGCAAAATCAACAAAAACACCGTCATTGTCGAAGCAACCAGCGGCAATACGGGTGTCGGTTTGGCA
ATGGTATGTGCCGCACGCGGCTACAAGCTGGCGATTACCATGCCGGAAAGCATGAGTAAGGAGCGCAAAATGCTGTTGCG
CGCGTTTGGTGCGGAGCTGATTCTGACCCCTGCCGCCGAAGGTATGGCGGGCGCGATTGCCAAAGCGAAATCCTTGGTGG
ACGCGCATCCCGACACTTATTTTATGCCGCGCCAGTTCGACAATGAGGCAAACCCCGAAGTCCACCGCAAAACAACCGCC
GAGGAAATTTGGCGGGATACGGACGGCAAAGTCGATGTCTTCGTTGCCGGCGTCGGCACGGGCGGTACGATTACCGGCGT
GGGCGAAGTGTTGAAAAAATACAAACCCGAAGTTAAAGTGGTTGCCGTCGAGCCTGAAGCTTCACCCGTATTGAGCGGCG
GCGAAAAAGGCCCGCACCCGATTCAAGGCATCGGCGCAGGCTTTATTCCGACCGTTTTGAATACCAAAATTTACGACAGC
ATTACCAAAGTGTCGAACGAAGCGGCTTTTGAAACCGCCCGCGCAATAGCGGAAAAAGAAGGCATTTTGGTGGGTATTTC
TTCCGGTGCGGCGGTTTGGAGTGCGTTGCAGCTTGCCAAACAGCCTGAAAACGAAGGCAAGCTGATAGTCGTGCTGCTGC
CTTCTTATGGCGAACGCTATCTCTCTACGCCACTTTTTGCAGATTTGGCATAA

Upstream 100 bases:

>100_bases
CGAAATTTGTTTATGTTTCGGCGCAAATCCCCTGCAATCGGACAGGATGCCTATGGGGATTGCGCCTTACTGTCGAAACC
TTATTATTCAGGAGCAGAAG

Downstream 100 bases:

>100_bases
TGCTTTAATCGGATTGTCGAAACATTCAGACGCATTTTTCGGTATCGGTGTAACGCCGTGCCGGAAAATGCGTTTTTGCA
TATATGCCGAAAACGCCGGT

Product: cysteine synthase

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVKDRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLA
MVCAARGYKLAITMPESMSKERKMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGPHPIQGIGAGFIPTVLNTKIYDS
ITKVSNEAAFETARAIAEKEGILVGISSGAAVWSALQLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA

Sequences:

>Translated_310_residues
MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVKDRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLA
MVCAARGYKLAITMPESMSKERKMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGPHPIQGIGAGFIPTVLNTKIYDS
ITKVSNEAAFETARAIAEKEGILVGISSGAAVWSALQLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA
>Mature_310_residues
MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVKDRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLA
MVCAARGYKLAITMPESMSKERKMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGPHPIQGIGAGFIPTVLNTKIYDS
ITKVSNEAAFETARAIAEKEGILVGISSGAAVWSALQLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family

Homologues:

Organism=Homo sapiens, GI295821202, Length=314, Percent_Identity=42.0382165605096, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI295821200, Length=314, Percent_Identity=42.0382165605096, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI4557415, Length=314, Percent_Identity=42.0382165605096, Blast_Score=233, Evalue=2e-61,
Organism=Escherichia coli, GI1788754, Length=313, Percent_Identity=56.2300319488818, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI2367138, Length=304, Percent_Identity=40.4605263157895, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1790207, Length=271, Percent_Identity=30.9963099630996, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17535051, Length=302, Percent_Identity=53.6423841059603, Blast_Score=313, Evalue=5e-86,
Organism=Caenorhabditis elegans, GI17562970, Length=302, Percent_Identity=50, Blast_Score=304, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI115535073, Length=310, Percent_Identity=54.8387096774194, Blast_Score=298, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI32566674, Length=294, Percent_Identity=50.6802721088435, Blast_Score=297, Evalue=6e-81,
Organism=Caenorhabditis elegans, GI17561720, Length=302, Percent_Identity=50.6622516556291, Blast_Score=270, Evalue=6e-73,
Organism=Caenorhabditis elegans, GI32566672, Length=216, Percent_Identity=48.6111111111111, Blast_Score=217, Evalue=7e-57,
Organism=Caenorhabditis elegans, GI17534315, Length=323, Percent_Identity=42.4148606811146, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI25147552, Length=319, Percent_Identity=38.871473354232, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17561716, Length=119, Percent_Identity=47.0588235294118, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17539800, Length=341, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71996324, Length=337, Percent_Identity=24.3323442136499, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321594, Length=317, Percent_Identity=37.5394321766561, Blast_Score=202, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6321449, Length=330, Percent_Identity=34.2424242424242, Blast_Score=134, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24643623, Length=311, Percent_Identity=40.1929260450161, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI20129101, Length=311, Percent_Identity=40.1929260450161, Blast_Score=200, Evalue=1e-51,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): CYSK_NEIMB (Q7DDL5)

Other databases:

- EMBL:   AE002098
- PIR:   H81161
- RefSeq:   NP_273805.1
- HSSP:   P47998
- ProteinModelPortal:   Q7DDL5
- SMR:   Q7DDL5
- EnsemblBacteria:   EBNEIT00000010907
- GeneID:   902878
- GenomeReviews:   AE002098_GR
- KEGG:   nme:NMB0763
- TIGR:   NMB0763
- GeneTree:   EBGT00050000020210
- HOGENOM:   HBG748215
- OMA:   ETIGDTP
- ProtClustDB:   CLSK877729
- BioCyc:   NMEN122586:NMB_0763-MONOMER
- BRENDA:   2.5.1.47
- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926
- TIGRFAMs:   TIGR01139
- TIGRFAMs:   TIGR01136

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =2.5.1.47

Molecular weight: Translated: 32821; Mature: 32821

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00901 CYS_SYNTHASE; PS00430 TONB_DEPENDENT_REC_1

Important sites: BINDING 74-74 BINDING 267-267

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVKDRIAEAMIEGAEKAGK
CCHHHHHHHHHCCCCEEEEHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCC
INKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKERKMLLRAFGAELILTPAAE
CCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEECCCC
GMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTAEEIWRDTDGKVDVFVAGVGT
CHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCC
GGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGPHPIQGIGAGFIPTVLNTKIYDS
CCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ITKVSNEAAFETARAIAEKEGILVGISSGAAVWSALQLAKQPENEGKLIVVLLPSYGERY
HHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHH
LSTPLFADLA
HCCCCHHCCC
>Mature Secondary Structure
MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVKDRIAEAMIEGAEKAGK
CCHHHHHHHHHCCCCEEEEHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCC
INKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKERKMLLRAFGAELILTPAAE
CCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEECCCC
GMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTAEEIWRDTDGKVDVFVAGVGT
CHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCC
GGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGPHPIQGIGAGFIPTVLNTKIYDS
CCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ITKVSNEAAFETARAIAEKEGILVGISSGAAVWSALQLAKQPENEGKLIVVLLPSYGERY
HHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHH
LSTPLFADLA
HCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10710307