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The map label for this gene is maeA [H]

Identifier: 161869608

GI number: 161869608

Start: 641213

End: 642493

Strand: Direct

Name: maeA [H]

Synonym: NMCC_0624

Alternate gene names: 161869608

Gene position: 641213-642493 (Clockwise)

Preceding gene: 161869607

Following gene: 161869609

Centisome position: 29.78

GC content: 55.04

Gene sequence:

>1281_bases
ATGGAAAACTCATTGAAAGAAGCCGCCCTCAAGTTCCACGAATTACCCGTTCCGGGCAAAATTTCCGTTACCCCGACCAA
ATCTTTGGCGACCGACAAAGATTTGGCGTTGGCGTACTCTCCGGGCGTAGCCGCTCCTTGTATGGAAATCCATGCCGATC
CGCAAAATGCCTACAAATACACCGCCAAAGGCAACTTGGTCGCCGTCATTTCCAACGGTACGGCCGTTTTGGGCTTGGGC
GACATCGGCGCGCTGGCGGGCAAACCCGTGATGGAAGGTAAAGGCGTATTGTTCAAAAAATTCGCCGGTGTGGACGTGTT
CGACATCGAAATCGATGAAAAAGACCCGCAAAAACTCGTGGACATCATCGCCGCTTTAGAGCCGACCTTCGGCGGCATCA
ACCTCGAAGACATCAAAGCACCCGAGTGTTTCTACATCGAACGCGAATTACGCAAACGCTGCAAAATCCCCGTATTCCAC
GACGACCAGCACGGCACGGCCATCATTACCGCCGCCGCCGTATTGAATGCCCTGCGTTTTACCGGCCGTAAAATCGAAGA
AGCGACTTTGGTGTGTTCCGGCGCAGGTGCGGCCGCGATTGCCTGCTTGAACCAATTGCTGGATTTGGGCTTGAAACGCG
AAAACGTGACCGTTTGCGACTCCAAAGGCGTGATTTACCAAACCCGCGAAGACAAAGACCGCATGGATGAGTCTAAACAG
TTCTACGCCATTGAAGACAACGGCCAGCGCGTGCTTGCCGATGCCGTCAAAGGCAAAGACATCTTCTTGGGCCTCTCCGG
CGCGAACCTGCTGACGCCTGAAATGCTGAACACCATGAACGAAAAACCCATCGTGTTCGCCATGGCCAACCCGAATCCGG
AAATTCTGCCGCCGCTGGCAAAAGAAACCCGTCCGGACGTGGTTATCGGTACCGGCCGTTCCGACTTCCCGAACCAAGTC
AACAATGTATTGTGCTTCCCGTTCATCTTCCGTGGCGCGTTGGATGTCGGCGCGACCACCATCAACGAAGAAATGAAACG
CGCCTGCGTGTATGCTTTGGCGGATTTGGCGATGGAAGAAGTGACCGAAGAAGTGGTTGCCGCTTACGGTAAGAAATTTG
AATTCGGCGCGGAATACCTGATTCCGACTCCGTTCGATTCCCGCCTGCTGCCGCGCGTCGCTACGGCTGCCGCCAAAGCA
GCGATGGAAAGCGGCGTGGCAACCCGTCCGATTGCCGACTTTGAAGCCTACGCCGCCAAGCTGGGCGAATGGAAGCTGTA
A

Upstream 100 bases:

>100_bases
AGGATGCGTCGGCAATATTAACGAACCGCCCGTAACATGATGACCCGAAAGCGTTTCGGACAGTCCGATTCAAATCTTTT
TCTCGCAACAGGATTGACAC

Downstream 100 bases:

>100_bases
GCCGTTTGTGGTTAAAATGCCGTCTGAACTGTTTTCAGGCGGCATTTTGCTGTCAGATTGATAAATGAAAGATACTGGAA
AATGAAAGAGATGGAGACTG

Product: malate oxidoreductase

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVAAPCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLG
DIGALAGKPVMEGKGVLFKKFAGVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTVCDSKGVIYQTREDKDRMDESKQ
FYAIEDNGQRVLADAVKGKDIFLGLSGANLLTPEMLNTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQV
NNVLCFPFIFRGALDVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPRVATAAAKA
AMESGVATRPIADFEAYAAKLGEWKL

Sequences:

>Translated_426_residues
MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVAAPCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLG
DIGALAGKPVMEGKGVLFKKFAGVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTVCDSKGVIYQTREDKDRMDESKQ
FYAIEDNGQRVLADAVKGKDIFLGLSGANLLTPEMLNTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQV
NNVLCFPFIFRGALDVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPRVATAAAKA
AMESGVATRPIADFEAYAAKLGEWKL
>Mature_426_residues
MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVAAPCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLG
DIGALAGKPVMEGKGVLFKKFAGVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTVCDSKGVIYQTREDKDRMDESKQ
FYAIEDNGQRVLADAVKGKDIFLGLSGANLLTPEMLNTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQV
NNVLCFPFIFRGALDVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPRVATAAAKA
AMESGVATRPIADFEAYAAKLGEWKL

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=356, Percent_Identity=26.9662921348315, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI62420880, Length=356, Percent_Identity=26.9662921348315, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI239049447, Length=356, Percent_Identity=26.9662921348315, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI4505143, Length=356, Percent_Identity=24.7191011235955, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1788806, Length=425, Percent_Identity=69.1764705882353, Blast_Score=610, Evalue=1e-176,
Organism=Saccharomyces cerevisiae, GI6322823, Length=236, Percent_Identity=28.8135593220339, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI281362674, Length=387, Percent_Identity=27.1317829457364, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21356279, Length=387, Percent_Identity=27.1317829457364, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281362672, Length=387, Percent_Identity=27.1317829457364, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281363503, Length=365, Percent_Identity=27.1232876712329, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI78707232, Length=365, Percent_Identity=27.1232876712329, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI281363505, Length=365, Percent_Identity=27.1232876712329, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI78707236, Length=365, Percent_Identity=27.1232876712329, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24646388, Length=410, Percent_Identity=25.609756097561, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24646386, Length=410, Percent_Identity=25.609756097561, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 46013; Mature: 46013

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVAAPCMEIHADPQNAYKY
CCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCHHHCCCCCHHCEEE
TAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFAGVDVFDIEIDEKDPQKLV
EECCCEEEEEECCEEEEEECCHHHHCCCCCCCCCCEEEEHCCCCEEEEEEECCCCHHHHH
DIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFHDDQHGTAIITAAAVLNALRF
HHHHHHCCCCCCCCHHHCCCCCEEEHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHH
TGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTVCDSKGVIYQTREDKDRMDESKQ
CCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEECCCCEEEECCCCHHHHCCCCE
FYAIEDNGQRVLADAVKGKDIFLGLSGANLLTPEMLNTMNEKPIVFAMANPNPEILPPLA
EEEECCCCCEEEHHHCCCCEEEEEECCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCHH
KETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGALDVGATTINEEMKRACVYALADLAMEE
HCCCCCEEEECCCCCCCHHHHCEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
VTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPRVATAAAKAAMESGVATRPIADFEAYAAK
HHHHHHHHHCCCEECCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LGEWKL
HCCCCC
>Mature Secondary Structure
MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVAAPCMEIHADPQNAYKY
CCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCHHHCCCCCHHCEEE
TAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFAGVDVFDIEIDEKDPQKLV
EECCCEEEEEECCEEEEEECCHHHHCCCCCCCCCCEEEEHCCCCEEEEEEECCCCHHHHH
DIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFHDDQHGTAIITAAAVLNALRF
HHHHHHCCCCCCCCHHHCCCCCEEEHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHH
TGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTVCDSKGVIYQTREDKDRMDESKQ
CCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEECCCCEEEECCCCHHHHCCCCE
FYAIEDNGQRVLADAVKGKDIFLGLSGANLLTPEMLNTMNEKPIVFAMANPNPEILPPLA
EEEECCCCCEEEHHHCCCCEEEEEECCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCHH
KETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGALDVGATTINEEMKRACVYALADLAMEE
HCCCCCEEEECCCCCCCHHHHCEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
VTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPRVATAAAKAAMESGVATRPIADFEAYAAK
HHHHHHHHHCCCEECCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LGEWKL
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376 [H]