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The map label for this gene is miaB [H]

Identifier: 161869349

GI number: 161869349

Start: 356139

End: 357467

Strand: Reverse

Name: miaB [H]

Synonym: NMCC_0355

Alternate gene names: 161869349

Gene position: 357467-356139 (Counterclockwise)

Preceding gene: 161869352

Following gene: 161869346

Centisome position: 16.6

GC content: 55.83

Gene sequence:

>1329_bases
ATGAAAAAAGTATTTATCCGTACCTTCGGCTGCCAGATGAACGAATACGACAGCGACAAAATGCTCGCCGTCCTCGCCGA
AGAACACGGCGGCATCGAACAGGTTACCCAAGCCGACGAAGCCGACATCATCTTGTTCAACACCTGCTCCGTGCGCGAAA
AAGCACAGGAAAAAGTGTTCTCCGATTTGGGGCGCGTGCGTCCGCTCAAAGAAAAAAACCCCGGCCTTATCATCGGCGTT
GCCGGCTGCGTCGCCTCGCAAGAAGGCGAAAACATCATCAAACGCGCGCCTTATGTTGACGTGGTTTTCGGCCCGCAAAC
GCTGCACCGCCTGCCCAAAATGATTGTGGACAAAGAAACCAGCGGGCTGTCGCAAGTCGATATTTCCTTTCCCGAAATCG
AAAAATTCGACCACCTGCCGCCCGCCCGCGTCGAAGGCGGCGCGGCATTTGTATCGATTATGGAAGGCTGTTCCAAATAC
TGCTCCTTCTGCGTCGTCCCCTACACGCGCGGCGAAGAATTCTCCCGCCCGCTCAACGACGTATTGACAGAAATCGCCAA
CCTTGCCCAGCAAGGCGTGAAAGAAATCAACCTCTTGGGACAAAACGTCAACGCCTATTGCGGCGAAATGGACGACGGCG
AAATCTGCGACTTCGCCACCCTGCTGCGCATCGTCCACGAAATCCCCGGCATCGAACGTATGCGCTTCACCACCAGCCAC
CCGCGCGAGTTTACCGACTCGATTATCGAGTGCTACCGCGACCTGCCCAAATTGGTTTCCCACCTGCACCTGCCGATTCA
AAGCGGTTCCGACCGCGTATTGAGCGCGATGAAACGCGGCTACACCGCTTTGGAATACAAATCCATCATCCGCAAACTGC
GTGCCATCCGTCCTGATTTATGCCTGAGCAGCGATTTCATCGTCGGCTTCCCCGGCGAGACCGAACGCGAGTTCGAGCAA
ACCTTGAAACTGGTGAAAGACATTGCCTTCGACTTGAGCTTCGTGTTTATTTACAGTCCGCGCCCCGGCACGCCTGCCGC
CAACCTGCACGACGACACGCCGCACGAAGAAAAAGTGCGCCGCCTCGAAGCCTTGAACGAAGTCATCGAAGCCGAAACCG
CGCGCATCAACCAAACGATGATCGGCACGGTTCAACGCTGCCTGGTCGAAGGCATCTCCAAAAAAGACCCCGACCAGCTC
CAAGCCCGCACCGCCAACAACCGCGTCGTCAACTTTACCGGCACGCCCAACATGATTAACCAAATGATCGATTTGGAAAT
CACCGAGGCCTACACCTTCTCCCTGCGCGGCAAAGTTGTCGAAGCCTAA

Upstream 100 bases:

>100_bases
CCACACCGATTTTCAGACGGCATCGTCATGCCGTCTGAAATTTTTTCATTCCAACAACCATCAGCCCCGCGATTACGGCT
GCCTGAGAAAGACACAAACC

Downstream 100 bases:

>100_bases
ACCCTTACGCCGAAAAAATGCCGTCTGAAGCGTTTCAGACGGCATTTTGCCTTGTATCGGCAGACGATGGCGCGGCCAGG
CGGCTTAATTTGCCGCATCC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADIILFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGV
AGCVASQEGENIIKRAPYVDVVFGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYCGEMDDGEICDFATLLRIVHEIPGIERMRFTTSH
PREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSAMKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQ
TLKLVKDIAFDLSFVFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGISKKDPDQL
QARTANNRVVNFTGTPNMINQMIDLEITEAYTFSLRGKVVEA

Sequences:

>Translated_442_residues
MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADIILFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGV
AGCVASQEGENIIKRAPYVDVVFGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYCGEMDDGEICDFATLLRIVHEIPGIERMRFTTSH
PREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSAMKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQ
TLKLVKDIAFDLSFVFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGISKKDPDQL
QARTANNRVVNFTGTPNMINQMIDLEITEAYTFSLRGKVVEA
>Mature_442_residues
MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADIILFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGV
AGCVASQEGENIIKRAPYVDVVFGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYCGEMDDGEICDFATLLRIVHEIPGIERMRFTTSH
PREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSAMKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQ
TLKLVKDIAFDLSFVFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGISKKDPDQL
QARTANNRVVNFTGTPNMINQMIDLEITEAYTFSLRGKVVEA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=482, Percent_Identity=35.8921161825726, Blast_Score=301, Evalue=7e-82,
Organism=Homo sapiens, GI28872782, Length=482, Percent_Identity=35.8921161825726, Blast_Score=301, Evalue=9e-82,
Organism=Homo sapiens, GI93277076, Length=454, Percent_Identity=27.9735682819383, Blast_Score=164, Evalue=2e-40,
Organism=Escherichia coli, GI1786882, Length=440, Percent_Identity=64.7727272727273, Blast_Score=580, Evalue=1e-167,
Organism=Escherichia coli, GI1787057, Length=389, Percent_Identity=31.3624678663239, Blast_Score=152, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=463, Percent_Identity=34.341252699784, Blast_Score=266, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI71996771, Length=308, Percent_Identity=31.4935064935065, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI21356207, Length=438, Percent_Identity=35.1598173515982, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI19922432, Length=460, Percent_Identity=27.3913043478261, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49566; Mature: 49566

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADIILFNTCSVREKAQEKVF
CCEEEEEEECCCCCCCCCCHHEEHEEHHCCCHHHHHCCCCCCEEEEECCCHHHHHHHHHH
SDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVVFGPQTLHRLPKMIVDKET
HHHHHCCCCCCCCCCEEEEEHHHHCCCCCCHHHHHCCEEEEEECHHHHHHHHHHHHCCCC
SGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKYCSFCVVPYTRGEEFSRPLND
CCCCEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCHHHCCCHHH
VLTEIANLAQQGVKEINLLGQNVNAYCGEMDDGEICDFATLLRIVHEIPGIERMRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCC
PREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSAMKRGYTALEYKSIIRKLRAIRPDL
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
CLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSFVFIYSPRPGTPAANLHDDTPHEEKVR
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCCHHHHHH
RLEALNEVIEAETARINQTMIGTVQRCLVEGISKKDPDQLQARTANNRVVNFTGTPNMIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEECCCHHHHH
QMIDLEITEAYTFSLRGKVVEA
HHHCEEEEEEEEEEECCEEECC
>Mature Secondary Structure
MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADIILFNTCSVREKAQEKVF
CCEEEEEEECCCCCCCCCCHHEEHEEHHCCCHHHHHCCCCCCEEEEECCCHHHHHHHHHH
SDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVVFGPQTLHRLPKMIVDKET
HHHHHCCCCCCCCCCEEEEEHHHHCCCCCCHHHHHCCEEEEEECHHHHHHHHHHHHCCCC
SGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKYCSFCVVPYTRGEEFSRPLND
CCCCEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCHHHCCCHHH
VLTEIANLAQQGVKEINLLGQNVNAYCGEMDDGEICDFATLLRIVHEIPGIERMRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCC
PREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSAMKRGYTALEYKSIIRKLRAIRPDL
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
CLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSFVFIYSPRPGTPAANLHDDTPHEEKVR
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCCHHHHHH
RLEALNEVIEAETARINQTMIGTVQRCLVEGISKKDPDQLQARTANNRVVNFTGTPNMIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEECCCHHHHH
QMIDLEITEAYTFSLRGKVVEA
HHHCEEEEEEEEEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA