Click here to switch to the map view.

The map label for this gene is trmE [H]

Identifier: 161869216

GI number: 161869216

Start: 228983

End: 230329

Strand: Reverse

Name: trmE [H]

Synonym: NMCC_0220

Alternate gene names: 161869216

Gene position: 230329-228983 (Counterclockwise)

Preceding gene: 161869220

Following gene: 161869212

Centisome position: 10.7

GC content: 59.76

Gene sequence:

>1347_bases
ATGTCGGATAACGTTCCAACGATTGCGGCAGTCGCTACCGCACCAGGGCGCGGCGGCGTGGGCGTGATACGCATATCGGG
GAAAAACCTGCTGCCGATGGCGCAGGCTTTGTGCGGGAAAACGCCCAAGCCGCGCGTGGCAACCTATGCTGATTTTACGG
ACGCGGACGGACAGGCAATCGACAGCGGGCTGCTACTGTTTTTTGCCGCACCGGCAAGTTTTACGGGCGAAGATGTCATC
GAGCTTCAGGGACACGGCGGGCCGGTGGTGATGGATATGCTGCTGAACCGCTGTTTGGAACTGGGCGCGCGCCTTGCCGA
ACCGGGCGAGTTTACCAAACGTGCGTTTTTGAACGACAAACTGGACTTGGCACAGGCGGAAGGTGTGGCGGATTTGATTG
ACGCATCCAGCCGTTCGGCGGCGCGTCTGGCGTTGCGCTCGCTCAAGGGCGATTTTTCGCGGCGGATACACGGTCTGGTC
GAAGATTTGATTACCTTGCGGATGCTGGTCGAAGCGACGCTGGATTTTCCCGAGGAAGACATTGATTTTCTCGAAGCGGC
GGACGCACGCGGCAAACTGGACGGCTTGCGCCGCGCCGTGGATGATGTGCTTGCCAACGCACAGCAGGGCGCGATTTTGC
GCGAAGGTCTGAATGTCGTATTGGTCGGTGCGCCGAATGTGGGCAAGTCGAGCCTGCTGAATGCTCTGGCGGGCGACGAA
GTGGCGATTGTTACCGATATTGCCGGAACGACGCGCGACGCAGTCAGGGAACGTATCCTGATTGACGGCGTGCCGGTGCA
TATTGTCGATACGGCAGGTTTGCGCGAGACGGACGACGTGGTCGAGCGTATCGGCATCGAACGCAGCCGCAAAGCCGTAT
CCGAAGCCGATGTCGCGCTGGTGTTGGTCGATCCGCGCGAGGGTTTGAATGAAAAGACACGGGCGATTTTGGACGCGCTG
CCGTCGGAGTTGAAACGCATCGAAATCCACAGCAAATCTGATTTGCATGCACACGCGGCAGGCGGGTTCGGTACGGGCGC
GGAAACCGTCATCGCGCTGTCGGCGAAAACTGGCGACGGCTTGGACGCGCTGAAACGGACGTTGTTGCGCGAGGCCGGAT
GGCAGGGCGAGGGCGAAGGGCTGTTTTTGGCGCGGACGCGGCACGTCAACGCACTCAAAGCAGCGCAGGAAGAATTGTCG
CTGGCGGCATTGTGCGGCAACCATCAAATCGAGCTGTTTGCCGAACACTTGCGCTTGGCGCAGGTCGCATGCGGCGAAAT
CACGGGCGAGTTTACGGCGGACGACCTGCTCGGCGTGATTTTTTCGAGGTTTTGTATCGGAAAATAA

Upstream 100 bases:

>100_bases
ATCAAGCATTTTTGCGGTTTTATGCCGTCTGAAAGCCAAACCGTCGGACTTCAGACGGCATTTGCTATAATCGCAGCTGT
TTTGAATTTTCGGGGGTTTT

Downstream 100 bases:

>100_bases
ACGGATCGAAAGCATCGTGGCGGTGTCCGGCTGAACATTCCGTTATCCCATAAAAACGGGAATCCGATCCGTTTGGTTTT
ATAGTGGATTAACAAAAATC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPKPRVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVI
ELQGHGGPVVMDMLLNRCLELGARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLNVVLVGAPNVGKSSLLNALAGDE
VAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRETDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDAL
PSELKRIEIHSKSDLHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGEGEGLFLARTRHVNALKAAQEELS
LAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK

Sequences:

>Translated_448_residues
MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPKPRVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVI
ELQGHGGPVVMDMLLNRCLELGARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLNVVLVGAPNVGKSSLLNALAGDE
VAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRETDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDAL
PSELKRIEIHSKSDLHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGEGEGLFLARTRHVNALKAAQEELS
LAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK
>Mature_447_residues
SDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPKPRVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIE
LQGHGGPVVMDMLLNRCLELGARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLVE
DLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLNVVLVGAPNVGKSSLLNALAGDEV
AIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRETDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDALP
SELKRIEIHSKSDLHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGEGEGLFLARTRHVNALKAAQEELSL
AALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=462, Percent_Identity=35.9307359307359, Blast_Score=213, Evalue=4e-55,
Organism=Homo sapiens, GI193082993, Length=452, Percent_Identity=35.8407079646018, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI193082988, Length=493, Percent_Identity=33.2657200811359, Blast_Score=199, Evalue=6e-51,
Organism=Escherichia coli, GI2367268, Length=459, Percent_Identity=54.9019607843137, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI87082120, Length=104, Percent_Identity=35.5769230769231, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17507259, Length=464, Percent_Identity=32.1120689655172, Blast_Score=182, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6323665, Length=491, Percent_Identity=32.5865580448065, Blast_Score=218, Evalue=2e-57,
Organism=Drosophila melanogaster, GI45550813, Length=472, Percent_Identity=33.4745762711864, Blast_Score=210, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 47491; Mature: 47360

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPKPRVATYADFTDADGQAI
CCCCCCEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCEEEECCCCCCCCCHH
DSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELGARLAEPGEFTKRAFLNDK
HCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
LDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLVEDLITLRMLVEATLDFPEED
HHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
IDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLNVVLVGAPNVGKSSLLNALAGDE
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCCHHHHHHHHCCCC
VAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRETDDVVERIGIERSRKAVSEADVAL
EEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHCCCCEEE
VLVDPREGLNEKTRAILDALPSELKRIEIHSKSDLHAHAAGGFGTGAETVIALSAKTGDG
EEECCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCEECCCCCCCCCCEEEEEECCCCCC
LDALKRTLLREAGWQGEGEGLFLARTRHVNALKAAQEELSLAALCGNHQIELFAEHLRLA
HHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QVACGEITGEFTADDLLGVIFSRFCIGK
HHHHHHCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPKPRVATYADFTDADGQAI
CCCCCEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCEEEECCCCCCCCCHH
DSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELGARLAEPGEFTKRAFLNDK
HCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
LDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLVEDLITLRMLVEATLDFPEED
HHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
IDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLNVVLVGAPNVGKSSLLNALAGDE
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCCHHHHHHHHCCCC
VAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRETDDVVERIGIERSRKAVSEADVAL
EEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHCCCCEEE
VLVDPREGLNEKTRAILDALPSELKRIEIHSKSDLHAHAAGGFGTGAETVIALSAKTGDG
EEECCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCEECCCCCCCCCCEEEEEECCCCCC
LDALKRTLLREAGWQGEGEGLFLARTRHVNALKAAQEELSLAALCGNHQIELFAEHLRLA
HHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QVACGEITGEFTADDLLGVIFSRFCIGK
HHHHHHCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA