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The map label for this gene is clcA [H]

Identifier: 161869195

GI number: 161869195

Start: 199942

End: 201264

Strand: Reverse

Name: clcA [H]

Synonym: NMCC_0198

Alternate gene names: 161869195

Gene position: 201264-199942 (Counterclockwise)

Preceding gene: 161869196

Following gene: 161869194

Centisome position: 9.35

GC content: 61.68

Gene sequence:

>1323_bases
TTGGCAGGTTCGGCACTCGTCGCCCTGACCGCGCTGTTTTTTGCCCATCTTGCCGATTTTGCGCTGGAACTGAACGCCAA
ACTGGTTCAACAATACCCGTGGTTCGCGTGGGTCGCGCTTCCTTTGGGTTTACCGCTTATTGCGTGGCTCACACGCAAAT
TCGCCCCCTTCACCGCCGGCAGCGGCATCCCGCAGGTCATCGCCTCACTGTCGCTGCCCTACGGCGCACAGAAAACGCGG
CTGATCCGCCTCGGGCAGACGCTGCTGAAGATTCCGCTGACCTTTTTGGGTATGCTGTTCGGCGCGTCCATCGGACGCGA
AGGTCCGTCCGTGCAGGTCGGCGCGGCAGTGATGGGCGCGTGGGGCGCGTGGTGCAAGAAACACGGCTTGGCATTCAAAG
GGATGCAGGAAAACGATTTGATGGCGGCGGGCGCGGCGGGCGGTTTGGCAGCCGCGTTCAACGCGCCGCTGGCGGGCGTG
ATTTTCGCCATTGAGGAACTCGGGCGCGGCATCATGTTGCGCTGGGAACGGCAGATTCTGCTCGGCGTACTCGCCGCCGG
TTTCATACAGGTCGCCATTCAGGGCAACAACCCGTATTTTTCCGGCTTCAACGGCGGCGTATTGGAACATATCTTTCTGT
GGGTCGCACTGTCCGGCCTGGTTTGCGGCGCGGCGGGCGGCCTGTTCGGACGTTTGCTCTATCGCGGTGCGGCGGCGTTT
GCACCGCGCAAGATACGCGGCTTCATCCGCAACCGTCCGCTGCTGCTGGCGGCACTGATGGGGCTGCTGCTCGCCCTGCT
CGGCACGTTCTACCAAGGCAAAACCTACGGCACCGGCTACCACGAAGCCGCCCAAGCCCTGCACGGCATCTACGAAGCCC
CCTTCGGACTCGCCGCCGCCAAATGGCTCGCCACCGTATTCAGCTATTGGGCAGGCGTTCCGGGCGGCATTTTCACTCCC
TCGCTGACCATAGGCGCGGTTTTGGGCGAGCATATCGCCGCCATCGCCGACATATCGCAGGGTGCAAACATCATCGTCCT
CATCTGCATGGCGGCATTTCTGGCGGGCGCGACACAATCCCCGATTACTTCCGCCGTCGTCGTCATGGAAATGACGGGCG
GACAAAGCCTGCTGTTTTGGATGCTAATTGCCTGCATTTTCGCCTCGCAGGTTTCGCGCCAGTTTTCGCCGCGTCCGTTC
TACCACGCATCGGGAATGCGCTTCCGCCAGCGCGTGCTTCAAGAAACCGCCGCCCAAACCGGCAATGCGCCCGCAAGACC
GCAAACAGCAAACAGCAAAACGGGAATGCCGTCTGAAAATTAA

Upstream 100 bases:

>100_bases
CGGACAACATGCCATGACCTCCACATTCCCCCGCCGCCTCGCCCGCAAAATCCGCCAAACCCGCCGCCTGTCGCGCAAAA
GCATCGCCTTTCTGTTCCTT

Downstream 100 bases:

>100_bases
AACGCCCCCGATCAAACGCCGGCAGCCGCCTTGATTTGAATACCGTTCCGCCGCCGCTTGAAACTTCAGCAACAATGCCG
TCTGAACGACAGAATGCGGT

Product: chloride channel protein-related protein

Products: Cl Ion [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MAGSALVALTALFFAHLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLPYGAQKTR
LIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLAFKGMQENDLMAAGAAGGLAAAFNAPLAGV
IFAIEELGRGIMLRWERQILLGVLAAGFIQVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAF
APRKIRGFIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLATVFSYWAGVPGGIFTP
SLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPITSAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPF
YHASGMRFRQRVLQETAAQTGNAPARPQTANSKTGMPSEN

Sequences:

>Translated_440_residues
MAGSALVALTALFFAHLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLPYGAQKTR
LIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLAFKGMQENDLMAAGAAGGLAAAFNAPLAGV
IFAIEELGRGIMLRWERQILLGVLAAGFIQVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAF
APRKIRGFIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLATVFSYWAGVPGGIFTP
SLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPITSAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPF
YHASGMRFRQRVLQETAAQTGNAPARPQTANSKTGMPSEN
>Mature_439_residues
AGSALVALTALFFAHLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLPYGAQKTRL
IRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLAFKGMQENDLMAAGAAGGLAAAFNAPLAGVI
FAIEELGRGIMLRWERQILLGVLAAGFIQVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFA
PRKIRGFIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLATVFSYWAGVPGGIFTPS
LTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPITSAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFY
HASGMRFRQRVLQETAAQTGNAPARPQTANSKTGMPSEN

Specific function: Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcA subfamily [H]

Homologues:

Organism=Homo sapiens, GI153252026, Length=466, Percent_Identity=23.6051502145923, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI55770840, Length=419, Percent_Identity=24.8210023866348, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI55770838, Length=419, Percent_Identity=24.8210023866348, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786350, Length=408, Percent_Identity=28.921568627451, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081943, Length=340, Percent_Identity=29.1176470588235, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322500, Length=386, Percent_Identity=23.8341968911917, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19922112, Length=430, Percent_Identity=26.046511627907, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 46517; Mature: 46386

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AGSALVALTALFFAHLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
SGIPQVIASLSLPYGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGA
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHH
WGAWCKKHGLAFKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQIL
HHHHHHHCCCEECCCCCCCCEECCCCCCHHHHHCCCHHHHHHHHHHHCCCCEEHHHHHHH
LGVLAAGFIQVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAF
HHHHHHHHHEEEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
APRKIRGFIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAA
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHH
KWLATVFSYWAGVPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQS
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
PITSAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETAAQT
CHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHC
GNAPARPQTANSKTGMPSEN
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cl Ion [Periplasm] [C]

Specific reaction: Cl Ion [Periplasm] = Cl Ion [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA