Click here to switch to the map view.

The map label for this gene is thiP [H]

Identifier: 161869176

GI number: 161869176

Start: 176163

End: 177581

Strand: Direct

Name: thiP [H]

Synonym: NMCC_0173

Alternate gene names: 161869176

Gene position: 176163-177581 (Clockwise)

Preceding gene: 161869173

Following gene: 161869177

Centisome position: 8.18

GC content: 60.68

Gene sequence:

>1419_bases
GTGCTGTCGGATGCCTATATGCTCAAACGTTTGGCGTGGACGGTATTTCAGGCAGCGGCAACCTGTGTGCTGGTGCTGCC
TTTGGGCGTGCCTGTCGCGTGGGTGCTGGCGCGGCTGGCGTTTCCGGGGCGGGCTTTGGTGCTGCGCCTGCTGATGCTGC
CTTTTGTGATGCCCACGTTGGTGGCGGGCGTGGGCGTGCTGGCTCTGTTTGGGGCGGACGGCCTGTTGTGGCGCGGACGG
CAGGATACGCCGTATCTGTTGTTGTACGGCAATGTGTTTTTCAACCTTCCTGTGTTGGTCAGGGCGGCGTATCAGGGGTT
TGTGCAAGTGCCTGCGGCACGGCTTCAGACGGCACGGACGTTGGGCGCGGGGGCGTGGCGGCGGTTTTTGGACATTGAAA
TGCCCGTTTTGCGCCCGTGGCTTGCCGGCGGCGTGTGCCTTGTCTTTCTGTATTGTTTTTCCGGGTTCGGGCTGGCGCTG
CTGCTGGGCGGCAGCCGTTATGCCACGGTCGAAGTGGAAATTTACCAGTTGGTCATGTTCGAACTCGATATGGCGGTTGC
TTCGGTGCTGGTGTGGCTGGTGTTGGGGGTAACGGCGGCGGCAGGGTTGCTGTATGCGTGGTTCGGCAGGCGCGCGGTTT
CGGATAAGGCGGTTGCCCCCGTGATGCCGTCGCCGCCGCAATCGGTGGAGGAATATGTGCTGCTGGCATTTTCGGCGGTG
GTGTTGTCCGTGTGCTGCCTGTTTCCTTTGTTGGCAATTGTTGTGAAAGCGTGGTCGGCCGGCGAATCGTGGCGTGTGTT
AATGGAAAGTGAAACGTGGCAGGCGGTGTGGAATACTTTGCGCTTCTCGACGGCGGCGGTGTTTGCGGCGGCGGTTTTGG
GTGTGGTGTATGCGGCGGCGGCGCGGCGGTCAGCGTGGATGCGCGGGCTGATGTTTTTGCCGTTTATGGTGTCGCCGGTT
TGTGTTTCGGCAGGCGTGCTGCTGCTTTATCCGGGGTGGACGGCTTCGTTGCCGTTGCTGCTGGCGATGTATGCGCTGCT
GGCGTATCCGTTTGTGGCAAAAGATGTTTTATCAGCCTGGGATGCACTGCCGCCGGATTACGGCAGGGCGGCGGCGGGTT
TGGGTGCAAACGGCTTTCAGACGGCATGCCGCATCACGTTCCCCCTCTTGAAACCGGCGTTGCGGCGCGGTCTGACTTTG
GCGGCGGCAACCTGCGTGGGCGAATTTGCGGCAACCTTGTTCCTGTCGCGTCCCGAGTGGCAGACGCTGACGACTTTGAT
TTATGCCTATTTGGGGCGTGCGGGTGAGGATAATTATGCGCGGGCAATGGTGTTGACATTGCTGTTGTCGGCGTTCGCGC
TGGGCATTTTCCTGCTGTTGGACAACGGCGAAGGCGGAAAACAGACGGAAACGTTATAA

Upstream 100 bases:

>100_bases
ATGGGGTGCTTTTGCCCTGCTGCCTTCGGCTTTTTTGGCGGTAATGGTCGTTGCGCCTTTGTGGGCGGTGGCGGCGTATG
ACGGTTTGGCGTGGCGCGCG

Downstream 100 bases:

>100_bases
TGTGAACCCTTTTTCAGAGGACGGCAAAATGAGCGAGTTGGATAACATCCTTGCCCATAACCGGCAGTTTGTCGAGTCGG
GCGAATATGAAAAATACTTT

Product: ABC transporter, permease protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MLSDAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLVAGVGVLALFGADGLLWRGR
QDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTARTLGAGAWRRFLDIEMPVLRPWLAGGVCLVFLYCFSGFGLAL
LLGGSRYATVEVEIYQLVMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVAPVMPSPPQSVEEYVLLAFSAV
VLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSTAAVFAAAVLGVVYAAAARRSAWMRGLMFLPFMVSPV
CVSAGVLLLYPGWTASLPLLLAMYALLAYPFVAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTL
AAATCVGEFAATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFALGIFLLLDNGEGGKQTETL

Sequences:

>Translated_472_residues
MLSDAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLVAGVGVLALFGADGLLWRGR
QDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTARTLGAGAWRRFLDIEMPVLRPWLAGGVCLVFLYCFSGFGLAL
LLGGSRYATVEVEIYQLVMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVAPVMPSPPQSVEEYVLLAFSAV
VLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSTAAVFAAAVLGVVYAAAARRSAWMRGLMFLPFMVSPV
CVSAGVLLLYPGWTASLPLLLAMYALLAYPFVAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTL
AAATCVGEFAATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFALGIFLLLDNGEGGKQTETL
>Mature_472_residues
MLSDAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLVAGVGVLALFGADGLLWRGR
QDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTARTLGAGAWRRFLDIEMPVLRPWLAGGVCLVFLYCFSGFGLAL
LLGGSRYATVEVEIYQLVMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVAPVMPSPPQSVEEYVLLAFSAV
VLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSTAAVFAAAVLGVVYAAAARRSAWMRGLMFLPFMVSPV
CVSAGVLLLYPGWTASLPLLLAMYALLAYPFVAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTL
AAATCVGEFAATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFALGIFLLLDNGEGGKQTETL

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=461, Percent_Identity=27.114967462039, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788764, Length=203, Percent_Identity=30.5418719211823, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787369, Length=153, Percent_Identity=32.6797385620915, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI87082099, Length=190, Percent_Identity=28.9473684210526, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 51078; Mature: 51078

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSDAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VAGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTART
HHHHHHHHHHCCCCCEECCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LGAGAWRRFLDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQLVMF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEHHHHHHHHH
ELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVAPVMPSPPQSVEEYVLLAFSAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSTAAVFAAAVLGVVYAAA
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPGWTASLPLLLAMYALLAYPFVAKDVLSAW
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEFAATLFLSRPEW
HCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCH
QTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFALGIFLLLDNGEGGKQTETL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCC
>Mature Secondary Structure
MLSDAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VAGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTART
HHHHHHHHHHCCCCCEECCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LGAGAWRRFLDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQLVMF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEHHHHHHHHH
ELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVAPVMPSPPQSVEEYVLLAFSAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSTAAVFAAAVLGVVYAAA
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPGWTASLPLLLAMYALLAYPFVAKDVLSAW
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEFAATLFLSRPEW
HCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCH
QTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFALGIFLLLDNGEGGKQTETL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA