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The map label for this gene is ligA [H]

Identifier: 161869144

GI number: 161869144

Start: 139440

End: 140264

Strand: Direct

Name: ligA [H]

Synonym: NMCC_0138

Alternate gene names: 161869144

Gene position: 139440-140264 (Clockwise)

Preceding gene: 161869142

Following gene: 161869145

Centisome position: 6.48

GC content: 57.33

Gene sequence:

>825_bases
ATGATTAAGAAGATAATCGGCGGCATCATACCGATTTTTACGGCGGTTTTCATCCCTGCATCGGCAGGCGCGGCGGATTT
GATGCTGGCGCAGGAATACAAAGGGCAGGACATTGCCGGCTGGGCGATGAGCGAGAAACTCGACGGCGTGCGCGCCTATT
GGGACGGAAAGCACCTGATAAGCCGTCAGGGGTATGCGTTTGCTCCGCCCAAAGGTTTTACCGCTCAGTTTCCGCCTTAT
CCTTTGGACGGCGAATTGTATAGCGGACGCGGTCAGTTCGAGCAGATTTCCGCTACCGTGCGTTCTGTTTCTTCAGACTG
GCGCGGCATCCGCCTGCACGTTTTCGATGTACCCAAGGCGCAGGGCAATCTCTACCAACGTTTGGCAGTCGCAACGCAGT
GGCTGAAAACGCATCCGAACGCGCCGATTACCATCATCCCGCAAATCAAAGTGCGCGACCGGCAGCACGCGATGGACTTT
TTAAAACAAATCGAAGCGCAGGGCGGCGAAGGCGTGATGCTGCGTCAGCCCGAATCCCGTTACAGCGGCGGCAGGAGCAG
CCAATTATTGAAGCTGAAAAGCCAATACGACGACGAATGCACGGTAACGCGGCACTATGAGGGCAAAGGGCGAAACGCCG
GACGGCTGGGCGCGGTCGGCTGCAAAAACCGACACGGCGAATTCCGCATCGGCAGCGGTTTCAAAGACAAAGACCGCGAC
AACCCGCCCAAAATCGGCACGCTGATTACCTATCGCTACCGTGGTTTTACGCGGAAAGGCACGCCGAAATTCGCCACGTT
TGTGCGCGCGCGTACCGACCGCTGA

Upstream 100 bases:

>100_bases
TAGGGTCATCGGCGTGTTCCTAAACGGTTTTTCAGACGGCATCGGGTTTGCCGTTTGTTTTATGGCGGTTTGCCGCCCGT
TTTGATATTGGGGGAATCAG

Downstream 100 bases:

>100_bases
GCAGGACAGTTCAGACGGCATCCGATACTTTGGTTTATAATTTTCCTTTTACCGACCGATTCCGACATATGACCGATTTA
GAAACCAAACGCCTTGAAAC

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MIKKIIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMSEKLDGVRAYWDGKHLISRQGYAFAPPKGFTAQFPPY
PLDGELYSGRGQFEQISATVRSVSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGAVGCKNRHGEFRIGSGFKDKDRD
NPPKIGTLITYRYRGFTRKGTPKFATFVRARTDR

Sequences:

>Translated_274_residues
MIKKIIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMSEKLDGVRAYWDGKHLISRQGYAFAPPKGFTAQFPPY
PLDGELYSGRGQFEQISATVRSVSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGAVGCKNRHGEFRIGSGFKDKDRD
NPPKIGTLITYRYRGFTRKGTPKFATFVRARTDR
>Mature_274_residues
MIKKIIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMSEKLDGVRAYWDGKHLISRQGYAFAPPKGFTAQFPPY
PLDGELYSGRGQFEQISATVRSVSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGAVGCKNRHGEFRIGSGFKDKDRD
NPPKIGTLITYRYRGFTRKGTPKFATFVRARTDR

Specific function: Catalyzes efficient strand joining on a single nicked DNA [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF01068 DNA_ligase_A_M [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 30679; Mature: 30679

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKIIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMSEKLDGVRAYWDGKHLI
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEECHHHHHHCCCEEEEECCCHHH
SRQGYAFAPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRSVSSDWRGIRLHVFDVPKA
HCCCCEECCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCC
QGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDFLKQIEAQGGEGVMLRQPESR
CCHHHHHHHHHHHHHHHCCCCCEEEEECEEECCHHHHHHHHHHHHCCCCCEEEEECCHHH
YSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGAVGCKNRHGEFRIGSGFKDKDRD
HCCCCCHHHEEHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCC
NPPKIGTLITYRYRGFTRKGTPKFATFVRARTDR
CCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MIKKIIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMSEKLDGVRAYWDGKHLI
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEECHHHHHHCCCEEEEECCCHHH
SRQGYAFAPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRSVSSDWRGIRLHVFDVPKA
HCCCCEECCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCC
QGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDFLKQIEAQGGEGVMLRQPESR
CCHHHHHHHHHHHHHHHCCCCCEEEEECEEECCHHHHHHHHHHHHCCCCCEEEEECCHHH
YSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGAVGCKNRHGEFRIGSGFKDKDRD
HCCCCCHHHEEHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCC
NPPKIGTLITYRYRGFTRKGTPKFATFVRARTDR
CCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550458; 7542800; 9060431 [H]