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The map label for this gene is thiE [H]

Identifier: 161869122

GI number: 161869122

Start: 116407

End: 117024

Strand: Reverse

Name: thiE [H]

Synonym: NMCC_0116

Alternate gene names: 161869122

Gene position: 117024-116407 (Counterclockwise)

Preceding gene: 161869123

Following gene: 161869121

Centisome position: 5.43

GC content: 62.14

Gene sequence:

>618_bases
ATGACCTTCCCGCCCCTAAAATCCCCGCTCAAATTCTACGCCGTCGTCCCCACCGCCGATTGGGTGGGGCGCATGGTCAA
AGCAGGTGCCGACACGGTGCAACTGCGCTGCAAGACCCTGCACGGCGATGAATTGAAACGCGAAATCGCCCGCTGCGTCG
CCGCCTGTCAAGGCAGCCGCACACAGCTTTTCATCAACGACCACTGGCACGAGGCAATCGAAGCGGGCGCATACGGCGTA
CATCTCGGGCAGGAAGACATGGACACCGCCGACCTTGCCGCCATCGCCGCCGCCGGTTTGCGTTTAGGTTTGAGTACGCA
CTCCGTCGCCGAACTCAACCGCGCCCTGTCCGTACATCCCAGCTACGTCGCCAGCGGCGCGATTTTCCCGACCACGACCA
AACAAATGCCCACCGCCCCGCAAGGCTTGGACAAACTGTGCGAGTATGTGAAACAGGCAGGCGGCACGCCCGTCGTCGCC
ATCGGCGGTATCGACTTGAACAACGCCCGAGCCGTACTCGCCACCGGCGTTTCCTCACTCGCCGCCGTCCGCGCCGTAAC
CGAAGCGGCAAATCCCGAAGCGGTGGTTAAAGCGTTTCAGGCTTTGTGGGATGGATAA

Upstream 100 bases:

>100_bases
AGTGGCACTGTTTGACGGAAAAGACGCGCCCGAACGCGATGAAGAAAGCGGTTTGGCGTATATCCGAAGACAAGATTAAA
GCCGCCCGAAAGGACACCTT

Downstream 100 bases:

>100_bases
AACCGAAAGAAGAAAATTCAATTGCCGTGTAGGCAAAACTTAGCCCGTTATCGCAAACATACTTAACTTTAAATGTGGCA
TATCATCAAATTCCGTCATT

Product: thiamin-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGDELKREIARCVAACQGSRTQLFINDHWHEAIEAGAYGV
HLGQEDMDTADLAAIAAAGLRLGLSTHSVAELNRALSVHPSYVASGAIFPTTTKQMPTAPQGLDKLCEYVKQAGGTPVVA
IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG

Sequences:

>Translated_205_residues
MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGDELKREIARCVAACQGSRTQLFINDHWHEAIEAGAYGV
HLGQEDMDTADLAAIAAAGLRLGLSTHSVAELNRALSVHPSYVASGAIFPTTTKQMPTAPQGLDKLCEYVKQAGGTPVVA
IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG
>Mature_204_residues
TFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGDELKREIARCVAACQGSRTQLFINDHWHEAIEAGAYGVH
LGQEDMDTADLAAIAAAGLRLGLSTHSVAELNRALSVHPSYVASGAIFPTTTKQMPTAPQGLDKLCEYVKQAGGTPVVAI
GGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=189, Percent_Identity=49.7354497354497, Blast_Score=189, Evalue=8e-50,
Organism=Saccharomyces cerevisiae, GI6325042, Length=198, Percent_Identity=28.7878787878788, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 21576; Mature: 21445

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGDELKREIARCVAACQGSR
CCCCCCCCCCEEEEEECCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCC
TQLFINDHWHEAIEAGAYGVHLGQEDMDTADLAAIAAAGLRLGLSTHSVAELNRALSVHP
EEEEECCHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
SYVASGAIFPTTTKQMPTAPQGLDKLCEYVKQAGGTPVVAIGGIDLNNARAVLATGVSSL
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCEECCCCHHHHHHHHHHH
AAVRAVTEAANPEAVVKAFQALWDG
HHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGDELKREIARCVAACQGSR
CCCCCCCCCEEEEEECCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCC
TQLFINDHWHEAIEAGAYGVHLGQEDMDTADLAAIAAAGLRLGLSTHSVAELNRALSVHP
EEEEECCHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
SYVASGAIFPTTTKQMPTAPQGLDKLCEYVKQAGGTPVVAIGGIDLNNARAVLATGVSSL
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCEECCCCHHHHHHHHHHH
AAVRAVTEAANPEAVVKAFQALWDG
HHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA