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The map label for this gene is exoA [H]

Identifier: 161869112

GI number: 161869112

Start: 105505

End: 106125

Strand: Direct

Name: exoA [H]

Synonym: NMCC_0103

Alternate gene names: 161869112

Gene position: 105505-106125 (Clockwise)

Preceding gene: 161869111

Following gene: 161869113

Centisome position: 4.9

GC content: 53.46

Gene sequence:

>621_bases
ATGCACGGCCATTGGCATTGCGCCGAGAAGCGCGGTTACAGCGGCGTGGCGGTGTACAGCAAACGCAAACCCGACAATGT
GCAAATCGGTATGGGCATTGAAGAATTCGACAGGGAAGGGCGGTTTGTGCGTTGCGATTTCGACAGGTTGAGCGTTATTT
CGCTTTACTTGCCCAGCGGCAGCAGCGCGGAAGAACGCCAGCAGGTGAAATACCGTTTCCTTGATGCGTTTTACCCTATG
CTCGAAGCGATGAAAAACGAAGGACGCGACATTGTCGTTTGCGGCGACTGGAACATCGCCCACCAAAACATCGACCTGAA
AAACTGGAAAGGCAATCAGAAAAATTCAGGTTTCCTGCCGGAAGAACGCGAGTGGATAGGCAAGGTTATCCACAAGCTCG
GTTGGACGGATATGTGGCGCACGCTTTATCCCGATGTGCCGGGCTACACGTGGTGGAGCAACCGCGGGCAGGCGTATGCG
AAAGATGTCGGGTGGCGCATCGATTATCAGATGGTTACGCCCGAACTTGCCGCCAAAGCCGTGTCCGCACACGTTTATAA
AGATGAAAAATTTTCCGACCATGCGCCGCTGGTCATGGAGTATGACTATGCTGCCGAATAA

Upstream 100 bases:

>100_bases
TTACGAATATATCGCCGCATCGGGCGCGGACATTGTCTGCGTGCAGGAACTCAAAGCGCAGGAGGCTGATTTGTCTGCCG
ATATGAAAAATCCGCACGGG

Downstream 100 bases:

>100_bases
AGTTTTGGGTAAATATGATTGGAATGTGGACGGAAAAACAGGAATAGGCGCAGCATGGGTTGTCGCTGCGTTTATCCTGC
CCATATTGGTGTGGGCGATA

Product: exodeoxyribonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDRLSVISLYLPSGSSAEERQQVKYRFLDAFYPM
LEAMKNEGRDIVVCGDWNIAHQNIDLKNWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYA
KDVGWRIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE

Sequences:

>Translated_206_residues
MHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDRLSVISLYLPSGSSAEERQQVKYRFLDAFYPM
LEAMKNEGRDIVVCGDWNIAHQNIDLKNWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYA
KDVGWRIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE
>Mature_206_residues
MHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDRLSVISLYLPSGSSAEERQQVKYRFLDAFYPM
LEAMKNEGRDIVVCGDWNIAHQNIDLKNWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYA
KDVGWRIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=200, Percent_Identity=36, Blast_Score=150, Evalue=7e-37,
Organism=Homo sapiens, GI18375503, Length=200, Percent_Identity=36, Blast_Score=150, Evalue=7e-37,
Organism=Homo sapiens, GI18375501, Length=200, Percent_Identity=36, Blast_Score=150, Evalue=7e-37,
Organism=Escherichia coli, GI1788046, Length=205, Percent_Identity=26.8292682926829, Blast_Score=74, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71989536, Length=193, Percent_Identity=34.7150259067358, Blast_Score=127, Evalue=5e-30,
Organism=Drosophila melanogaster, GI221330655, Length=205, Percent_Identity=36.5853658536585, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI17136678, Length=205, Percent_Identity=36.5853658536585, Blast_Score=138, Evalue=2e-33,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 24036; Mature: 24036

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDRLSVISLYLPSG
CCCCCCCHHHCCCCCEEEEECCCCCCEEEECCHHHHCCCCCEEEECCCCEEEEEEECCCC
SSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLKNWKGNQKNSGFLP
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCCC
EEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGWRIDYQMVTPELAAKA
HHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEECCCCCEEHHCCCEEEEEEEECHHHHHHH
VSAHVYKDEKFSDHAPLVMEYDYAAE
HHHHHCCCCCCCCCCCEEEECCCCCC
>Mature Secondary Structure
MHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDRLSVISLYLPSG
CCCCCCCHHHCCCCCEEEEECCCCCCEEEECCHHHHCCCCCEEEECCCCEEEEEEECCCC
SSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLKNWKGNQKNSGFLP
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCCC
EEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGWRIDYQMVTPELAAKA
HHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEECCCCCEEHHCCCEEEEEEEECHHHHHHH
VSAHVYKDEKFSDHAPLVMEYDYAAE
HHHHHCCCCCCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]