Click here to switch to the map view.

The map label for this gene is metG [H]

Identifier: 161869048

GI number: 161869048

Start: 23222

End: 25384

Strand: Reverse

Name: metG [H]

Synonym: NMCC_0031

Alternate gene names: 161869048

Gene position: 25384-23222 (Counterclockwise)

Preceding gene: 161869049

Following gene: 161869046

Centisome position: 1.18

GC content: 54.14

Gene sequence:

>2163_bases
TTGGCAAAAATGCCGTCTGAAGCCCGATTTTTCAGGCTTCAGACGGCATTTCCCCGTTTGATTTGCGGTATAATCCGCCT
TTACCCTTTGTTTGCAAAGCACAATATGACACGCAAAATCTTAGTTACCTCCGCCCTGCCCTATGCCAACGGCAGCATCC
ACCTCGGCCACATGGTCGAACACATCCAAACCGACGTTTGGGTACGCTTCCAAAAACTGCGCGGCAACGAATGCCACTAC
TGCTGCGCCGACGACACCCACGGCACGCCCGTGATGCTTGCCGCGCAAAAACAAGGTATCGCCCCCGAAGACATGATTGC
CAAAGTGCGCGAAGAGCACCTCGCCGACTTTACTGGCTTTTTCATCGGTTACGACAATTATTACAGCACCCATTCCCCTG
AAAACAAACAGTTTTCCCAAGACATTTACCGCGCGCTGAAAGCCAACGGCAAGATTGAGAGCCGCGTCATCGAGCAGCTT
TTCGACCCCGAAAAACAAATGTTCCTGCCCGACCGCTTCGTCAAAGGCGAATGCCCGAAATGCCACGCCCAAGACCAATA
CGGCGACAACTGCGAAGTCTGCGGCACGACCTATTCCCCGACCGAACTGATTAATCCGTATTCCGCCGTTTCCGGTGCCA
AACCCGAATTGCGCGAATCCGAACACTTCTTCTTCAAACTGGGAGAATGCGCCGACTTCCTCAAAGCATGGACTTCCGGC
AACAACCCGCACGACGGCAAGCCCCATCTGCAAGCCGAAGCCCTCAACAAAATGAAAGAATGGCTGGGCGAAGGTGAAGA
AACCACCCTGTCCGACTGGGATATTTCCCGCGACGCGCCGTATTTCGGTTTTGAAATCCCCGACGCGCCGGGCAAATACT
TCTACGTCTGGCTGGACGCGCCCGTCGGCTACATGGCGTCGTTTAAAAACCTGTGCGACCGCATCGGCGTCGATTTCGAC
GAATACTTCAAAGCCGGCAGCCAAACCGAGATGTACCACTTCATCGGCAAAGACATCCTCTATTTCCACGCCCTGTTCTG
GCCTGCCATGCTGCATTTCTCCGGCCACCGCGCCCCGACCGGCGTGTACGCACACGGCTTTTTGACCGTGGACGGACAAA
AAATGTCCAAATCGCGCGGCACGTTTATCACCGCCAAATCCTATTTGGAACAAGGTTTAAACCCCGAGTGGATGCGCTAC
TACATCGCCGCCAAACTCAACAGCAAAATCGAAGACATCGATTTGAACCTGCAAGACTTTATCAGCCGCGTAAACAGCGA
CCTTGTCGGCAAATACGTCAACATCGCCGCCCGCGCCTCAGGTTTTATCGCCAAACGCTTTGAAGGCCGTCTGAAAGACG
TTGCCGACAGCGCATTGCTGGCAAAACTTGCCGCCGAAAGCGACACCATCGCCGAGCAATACGAAAACCGCGAATACGCC
CGCGCCCTGCGCGATATCATGGCATTGGCAGATGCCGTGAACGAATACGTTGATGCCAACAAACCTTGGGAACTGGCGAA
ACAGGAAGGTCAAGACGAACGCCTGCACGAAGTGTGCAGCGAGCTCATCAACGCCTTCACCATGTTGACCGCCTACCTCG
CCCCCGTCTTGCCGCAAACCGCCGCAAACGCCGCCAAATTCCTGAATTTGGAAGCCATCACTTGGGCAAATACACGCGAA
ACCTTGGGCAAACACGCCATCAACAAATACAAACATTTAATGCAACGAGTGGAGCAAAAACAAGTGGACGATTTAATCGA
AGCCAACAAACAAAGCATCGCCGCCGCAGCCGCACCTGCCGCCGAAGAGAGCAAATACGAAAAAGTCGCCGAACAGGCGA
GCTTCGACGACTTTATGAAAATCGACATGCGCGTCGCCAAAGTATTAAACTGCGAAGCCGTCGAAGGCAGCACCAAACTC
CTGAAATTCGACCTCGATTTCGGTTTTGAGCAACGCATCATCTTCTCCGGCATCGCCGCGTCTTACCTGAACCCTGCCGA
GTTAAACGGAAGGATGGTCATCGCCGTCGCCAACTTCGCCCCGCGCAAAATGGCGAAATTCGGCGTATCCGAAGGCATGA
TTTTGAGTGCCGCCACGGCAGACGGGAAACTGAAACTGCTTGATGTCGACGCAGGCGCGCAACCGGGCGACAAAGTCGGT
TAA

Upstream 100 bases:

>100_bases
GTGCAGCTCCTCGCCTACCACACCGCCCTCGCGCGCGGCACGGATGTAGACAAGCCGCGCAACCTGGCGAAATCCGTAAC
CGTCGAATAAATACCGCCGG

Downstream 100 bases:

>100_bases
GTAGAAATATCAAAAACAATGCCGTCTGGACCCTTGAGGCCTTCAGACGGCATTGTTTTCCCATAATCCCGACAAGCTCA
AACCACCCGATTTTGTGCCT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 720; Mature: 719

Protein sequence:

>720_residues
MAKMPSEARFFRLQTAFPRLICGIIRLYPLFAKHNMTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGNECHY
CCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFFIGYDNYYSTHSPENKQFSQDIYRALKANGKIESRVIEQL
FDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCGTTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSG
NNPHDGKPHLQAEALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNLCDRIGVDFD
EYFKAGSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLTVDGQKMSKSRGTFITAKSYLEQGLNPEWMRY
YIAAKLNSKIEDIDLNLQDFISRVNSDLVGKYVNIAARASGFIAKRFEGRLKDVADSALLAKLAAESDTIAEQYENREYA
RALRDIMALADAVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAANAAKFLNLEAITWANTRE
TLGKHAINKYKHLMQRVEQKQVDDLIEANKQSIAAAAAPAAEESKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKL
LKFDLDFGFEQRIIFSGIAASYLNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGDKVG

Sequences:

>Translated_720_residues
MAKMPSEARFFRLQTAFPRLICGIIRLYPLFAKHNMTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGNECHY
CCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFFIGYDNYYSTHSPENKQFSQDIYRALKANGKIESRVIEQL
FDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCGTTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSG
NNPHDGKPHLQAEALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNLCDRIGVDFD
EYFKAGSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLTVDGQKMSKSRGTFITAKSYLEQGLNPEWMRY
YIAAKLNSKIEDIDLNLQDFISRVNSDLVGKYVNIAARASGFIAKRFEGRLKDVADSALLAKLAAESDTIAEQYENREYA
RALRDIMALADAVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAANAAKFLNLEAITWANTRE
TLGKHAINKYKHLMQRVEQKQVDDLIEANKQSIAAAAAPAAEESKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKL
LKFDLDFGFEQRIIFSGIAASYLNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGDKVG
>Mature_719_residues
AKMPSEARFFRLQTAFPRLICGIIRLYPLFAKHNMTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGNECHYC
CADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFFIGYDNYYSTHSPENKQFSQDIYRALKANGKIESRVIEQLF
DPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCGTTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGN
NPHDGKPHLQAEALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNLCDRIGVDFDE
YFKAGSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLTVDGQKMSKSRGTFITAKSYLEQGLNPEWMRYY
IAAKLNSKIEDIDLNLQDFISRVNSDLVGKYVNIAARASGFIAKRFEGRLKDVADSALLAKLAAESDTIAEQYENREYAR
ALRDIMALADAVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAANAAKFLNLEAITWANTRET
LGKHAINKYKHLMQRVEQKQVDDLIEANKQSIAAAAAPAAEESKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLL
KFDLDFGFEQRIIFSGIAASYLNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGDKVG

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=589, Percent_Identity=28.6926994906621, Blast_Score=235, Evalue=9e-62,
Organism=Homo sapiens, GI24308436, Length=542, Percent_Identity=25.830258302583, Blast_Score=145, Evalue=2e-34,
Organism=Escherichia coli, GI1788432, Length=687, Percent_Identity=56.3318777292576, Blast_Score=806, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=589, Percent_Identity=26.3157894736842, Blast_Score=219, Evalue=5e-57,
Organism=Caenorhabditis elegans, GI212645096, Length=518, Percent_Identity=22.972972972973, Blast_Score=130, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71992781, Length=346, Percent_Identity=24.2774566473988, Blast_Score=97, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321704, Length=574, Percent_Identity=28.5714285714286, Blast_Score=232, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6321610, Length=543, Percent_Identity=23.0202578268877, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24655311, Length=631, Percent_Identity=29.0015847860539, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI21358483, Length=527, Percent_Identity=26.1859582542694, Blast_Score=145, Evalue=1e-34,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 80916; Mature: 80785

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMPSEARFFRLQTAFPRLICGIIRLYPLFAKHNMTRKILVTSALPYANGSIHLGHMVE
CCCCCCHHHHEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEHHHHHH
HIQTDVWVRFQKLRGNECHYCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGF
HHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
FIGYDNYYSTHSPENKQFSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPK
EEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHCCCCHHCCCCCCC
CHAQDQYGDNCEVCGTTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSG
CCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCCCCCCCCCCCHHEEEHHHHHHHHHHHHCC
NNPHDGKPHLQAEALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDA
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEEEEECC
PVGYMASFKNLCDRIGVDFDEYFKAGSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPT
CHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GVYAHGFLTVDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDF
CEEEEEEEEECCHHHHCCCCCEEEEHHHHHHCCCHHHHHHHHHHHHCCCHHHCCCCHHHH
ISRVNSDLVGKYVNIAARASGFIAKRFEGRLKDVADSALLAKLAAESDTIAEQYENREYA
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHH
RALRDIMALADAVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQT
HHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AANAAKFLNLEAITWANTRETLGKHAINKYKHLMQRVEQKQVDDLIEANKQSIAAAAAPA
HHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
AEESKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEQRIIFSGIAA
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
SYLNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGDKVG
HHCCHHHCCCEEEEEECCCCHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
AKMPSEARFFRLQTAFPRLICGIIRLYPLFAKHNMTRKILVTSALPYANGSIHLGHMVE
CCCCCHHHHEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEHHHHHH
HIQTDVWVRFQKLRGNECHYCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGF
HHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
FIGYDNYYSTHSPENKQFSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPK
EEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHCCCCHHCCCCCCC
CHAQDQYGDNCEVCGTTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSG
CCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCCCCCCCCCCCHHEEEHHHHHHHHHHHHCC
NNPHDGKPHLQAEALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDA
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEEEEECC
PVGYMASFKNLCDRIGVDFDEYFKAGSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPT
CHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GVYAHGFLTVDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDF
CEEEEEEEEECCHHHHCCCCCEEEEHHHHHHCCCHHHHHHHHHHHHCCCHHHCCCCHHHH
ISRVNSDLVGKYVNIAARASGFIAKRFEGRLKDVADSALLAKLAAESDTIAEQYENREYA
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHH
RALRDIMALADAVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQT
HHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AANAAKFLNLEAITWANTRETLGKHAINKYKHLMQRVEQKQVDDLIEANKQSIAAAAAPA
HHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
AEESKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEQRIIFSGIAA
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
SYLNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGDKVG
HHCCHHHCCCEEEEEECCCCHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA