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The map label for this gene is thiG [H]

Identifier: 161777624

GI number: 161777624

Start: 2831153

End: 2832142

Strand: Reverse

Name: thiG [H]

Synonym: Rru_A2432

Alternate gene names: 161777624

Gene position: 2832142-2831153 (Counterclockwise)

Preceding gene: 83593772

Following gene: 83593766

Centisome position: 65.06

GC content: 66.67

Gene sequence:

>990_bases
ATGCGGGTGATCATCAACGGCGAGCCGCGCGAGCTCGCCCCCTCCGTTTCCATCGAGGCCTTGCTGGTTGGCATGGGGGT
CGATCCCCGTAAGGTGGCGGTCGAGCGCAACCGCGAGATCGTGGCGCGGACCACTTATAAGGAAACCGCCCTTGATGAGG
GCGATCAGCTTGAAATCGTCCATTTCATCGGTGGCGGCGACCGCTCGGACCCCCAGCCGGCCGATGATCCGTTCACCGTG
GCCGGTCGGGTGTTCTCGTCGCGGCTGCTGGTCGGCACCGGCAAATACCGCGATTTCGAGGAGACCGCCCGGGCGATCGA
AGCCAGCGGCGCCGAAATCGTCACCGTCGCCGTGCGCCGGGTCAATGTCAGCGATCCGTCGCAGCCGATGCTGGTCGATT
ACGTCGATCCCGCCAAATACACCTATCTGCCCAATACGGCCGGCTGTTTCACCGCCGACGAGGCCGTGCGCACCCTGCGG
CTGGCCCGCGAGGCCGGCGGCTGGTCGCTGGTCAAGCTCGAGGTTCTCGGCGATCAGCGCACGCTTTATCCCAATATGGC
CGAGACCTTCCTGGCCGCCGAGGCCCTGATTCGCGACGGCTTCGAGGTGATGGTCTATTGCGCCGATGATCCGATCGCCG
CCAAGCGGCTGGAGGATATGGGCTGCGTGGCGATCATGCCGCTGGGGTCGCTGATCGGCTCGGGGCTGGGTATCATCAAC
CCCTATACCATCCGCCTGATCAAGGAGAGCGTCAGCGTTCCCGTGCTGGTCGACGCCGGGGTGGGAACCGCCTCCGACGC
CGCCGTGGCCATGGAACTGGGCTGCGACGGCGTGTTGATGAACACGGCCATCGCCCAGGCCAAGGACCCGGTGCGCATGG
CGCGCGCCATGCGGCTGGCGATCGAGGCCGGGCGGCTGAGCTATCAGGCCGGCCGCATGCCGCGCAAACTCTACGCCGAT
CCGTCCTCGCCGCTGGCCGGGCTGATCTAG

Upstream 100 bases:

>100_bases
CGAGGGCCGTCTCTCCGCCCAGAAAAAGCGGGATCGCTTTCGCGGAGGGGCCGCCCCGGATTGAGAGCCCGCCCAACAAA
CAGCCATGGAATTCCAAGAC

Downstream 100 bases:

>100_bases
GCCCGCCGCCAAGCCGGTCGTTTTTACCCGATGGGGCGGCAGGTTTTGCCGCCCCATCGGCGTTTTGATCCTGACGAGCA
CAAGACCTTGGCTGAAAGGT

Product: bifunctional sulfur carrier protein/thiazole synthase protein

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MRVIINGEPRELAPSVSIEALLVGMGVDPRKVAVERNREIVARTTYKETALDEGDQLEIVHFIGGGDRSDPQPADDPFTV
AGRVFSSRLLVGTGKYRDFEETARAIEASGAEIVTVAVRRVNVSDPSQPMLVDYVDPAKYTYLPNTAGCFTADEAVRTLR
LAREAGGWSLVKLEVLGDQRTLYPNMAETFLAAEALIRDGFEVMVYCADDPIAAKRLEDMGCVAIMPLGSLIGSGLGIIN
PYTIRLIKESVSVPVLVDAGVGTASDAAVAMELGCDGVLMNTAIAQAKDPVRMARAMRLAIEAGRLSYQAGRMPRKLYAD
PSSPLAGLI

Sequences:

>Translated_329_residues
MRVIINGEPRELAPSVSIEALLVGMGVDPRKVAVERNREIVARTTYKETALDEGDQLEIVHFIGGGDRSDPQPADDPFTV
AGRVFSSRLLVGTGKYRDFEETARAIEASGAEIVTVAVRRVNVSDPSQPMLVDYVDPAKYTYLPNTAGCFTADEAVRTLR
LAREAGGWSLVKLEVLGDQRTLYPNMAETFLAAEALIRDGFEVMVYCADDPIAAKRLEDMGCVAIMPLGSLIGSGLGIIN
PYTIRLIKESVSVPVLVDAGVGTASDAAVAMELGCDGVLMNTAIAQAKDPVRMARAMRLAIEAGRLSYQAGRMPRKLYAD
PSSPLAGLI
>Mature_329_residues
MRVIINGEPRELAPSVSIEALLVGMGVDPRKVAVERNREIVARTTYKETALDEGDQLEIVHFIGGGDRSDPQPADDPFTV
AGRVFSSRLLVGTGKYRDFEETARAIEASGAEIVTVAVRRVNVSDPSQPMLVDYVDPAKYTYLPNTAGCFTADEAVRTLR
LAREAGGWSLVKLEVLGDQRTLYPNMAETFLAAEALIRDGFEVMVYCADDPIAAKRLEDMGCVAIMPLGSLIGSGLGIIN
PYTIRLIKESVSVPVLVDAGVGTASDAAVAMELGCDGVLMNTAIAQAKDPVRMARAMRLAIEAGRLSYQAGRMPRKLYAD
PSSPLAGLI

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=250, Percent_Identity=46.4, Blast_Score=219, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR012675
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 35317; Mature: 35317

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVIINGEPRELAPSVSIEALLVGMGVDPRKVAVERNREIVARTTYKETALDEGDQLEIV
CEEEECCCCHHHCCCCCEEEEEEECCCCHHHHHHHCCCEEEEEECHHHHHCCCCCCEEEE
HFIGGGDRSDPQPADDPFTVAGRVFSSRLLVGTGKYRDFEETARAIEASGAEIVTVAVRR
EEECCCCCCCCCCCCCCHHHHHHHHHCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEEE
VNVSDPSQPMLVDYVDPAKYTYLPNTAGCFTADEAVRTLRLAREAGGWSLVKLEVLGDQR
ECCCCCCCCEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCC
TLYPNMAETFLAAEALIRDGFEVMVYCADDPIAAKRLEDMGCVAIMPLGSLIGSGLGIIN
EECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCEEEEEHHHHHHCCCCCCC
PYTIRLIKESVSVPVLVDAGVGTASDAAVAMELGCDGVLMNTAIAQAKDPVRMARAMRLA
CEEEEEEHHCCCCEEEEECCCCCCCCCEEEEEECCCCEEHHHHHHHCCCHHHHHHHHHHH
IEAGRLSYQAGRMPRKLYADPSSPLAGLI
HHHCCEEECCCCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRVIINGEPRELAPSVSIEALLVGMGVDPRKVAVERNREIVARTTYKETALDEGDQLEIV
CEEEECCCCHHHCCCCCEEEEEEECCCCHHHHHHHCCCEEEEEECHHHHHCCCCCCEEEE
HFIGGGDRSDPQPADDPFTVAGRVFSSRLLVGTGKYRDFEETARAIEASGAEIVTVAVRR
EEECCCCCCCCCCCCCCHHHHHHHHHCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEEE
VNVSDPSQPMLVDYVDPAKYTYLPNTAGCFTADEAVRTLRLAREAGGWSLVKLEVLGDQR
ECCCCCCCCEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCC
TLYPNMAETFLAAEALIRDGFEVMVYCADDPIAAKRLEDMGCVAIMPLGSLIGSGLGIIN
EECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCEEEEEHHHHHHCCCCCCC
PYTIRLIKESVSVPVLVDAGVGTASDAAVAMELGCDGVLMNTAIAQAKDPVRMARAMRLA
CEEEEEEHHCCCCEEEEECCCCCCCCCEEEEEECCCCEEHHHHHHHCCCHHHHHHHHHHH
IEAGRLSYQAGRMPRKLYADPSSPLAGLI
HHHCCEEECCCCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA