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The map label for this gene is truB

Identifier: 161777478

GI number: 161777478

Start: 342491

End: 343210

Strand: Reverse

Name: truB

Synonym: SSO0393

Alternate gene names: 161777478

Gene position: 343210-342491 (Counterclockwise)

Preceding gene: 15897326

Following gene: 15897324

Centisome position: 11.47

GC content: 35.42

Gene sequence:

>720_bases
ATGAACTATATAAGTAAAGGAGGAAAAGAGTACGTATGTGTAATGCAAGTTCACTGTGAATACAATAAAGAGGAATTGGC
AAAGATAATAAGTTCATTTAAAGGAGAAATATACCAACGACCACCAGTTAGATCATCAGTCAAAAGGAGACTAAGAATTA
GAAGAATATATGATATAGAAATTCTAGACATGGACAAGAAGTTAGTATTACTTAGAGTAAACTGTGACTCCGGGACGTAC
ATGAGAAAATTATGTCATGATATAGGAATAATTTACGGTTGTGGAGCTCATATGAGAGAGCTAAGAAGAACTAGATCCGG
GATTTTCACAGAATCCACAAATCTAGTGAAATTACACGAACTTTCTGAAGCAATATACTTATACAAGAACTGCAAAGACG
AAACAGAACTAAGAAGAGTACTTATACCAATGGAATTCGCAACTTGTGAAATTCCAAAAATTGTAATAGAAGACTCAGCT
GTAAACGCATTAGCTTATGGTGCGCAACTAGCAGTACCTGGAGTAGTAGCTTATCAGAACTTTAAAAAGAACGATACAGT
CGCAGTATTAACGCTTAAAGGGGAATTAGTAGCGACAGGCAACGCACTAATGGACAGCGAAGAATTGGAAAACGCAAAAA
AGGGAATAGTAGTCAACTTAAGTAGAGTTTTTATGCAGAGAGATATATATCCAAAAGCATGGAAAAAACATGAGTCATGA

Upstream 100 bases:

>100_bases
AAAGCAGGACATGGGGGGACCCTAGAGCCCATTCATTGGGCGGGGTAATCCTAAAGTTACCGGTGTGTTACCAATAGGAA
TAGAGAACGCAACTAAAATA

Downstream 100 bases:

>100_bases
GTACATAATTTTTGAAGTAATTAACGGTATACCTCTAACCCTTCTATCCTCACCGGGGATATTTTCAAAAAAGGAACTAG
ATTTGGGAACAAGGATACTT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MNYISKGGKEYVCVMQVHCEYNKEELAKIISSFKGEIYQRPPVRSSVKRRLRIRRIYDIEILDMDKKLVLLRVNCDSGTY
MRKLCHDIGIIYGCGAHMRELRRTRSGIFTESTNLVKLHELSEAIYLYKNCKDETELRRVLIPMEFATCEIPKIVIEDSA
VNALAYGAQLAVPGVVAYQNFKKNDTVAVLTLKGELVATGNALMDSEELENAKKGIVVNLSRVFMQRDIYPKAWKKHES

Sequences:

>Translated_239_residues
MNYISKGGKEYVCVMQVHCEYNKEELAKIISSFKGEIYQRPPVRSSVKRRLRIRRIYDIEILDMDKKLVLLRVNCDSGTY
MRKLCHDIGIIYGCGAHMRELRRTRSGIFTESTNLVKLHELSEAIYLYKNCKDETELRRVLIPMEFATCEIPKIVIEDSA
VNALAYGAQLAVPGVVAYQNFKKNDTVAVLTLKGELVATGNALMDSEELENAKKGIVVNLSRVFMQRDIYPKAWKKHES
>Mature_239_residues
MNYISKGGKEYVCVMQVHCEYNKEELAKIISSFKGEIYQRPPVRSSVKRRLRIRRIYDIEILDMDKKLVLLRVNCDSGTY
MRKLCHDIGIIYGCGAHMRELRRTRSGIFTESTNLVKLHELSEAIYLYKNCKDETELRRVLIPMEFATCEIPKIVIEDSA
VNALAYGAQLAVPGVVAYQNFKKNDTVAVLTLKGELVATGNALMDSEELENAKKGIVVNLSRVFMQRDIYPKAWKKHES

Specific function: Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI215599015, Length=232, Percent_Identity=39.2241379310345, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI4503337, Length=232, Percent_Identity=39.2241379310345, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17553978, Length=232, Percent_Identity=34.9137931034483, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6323204, Length=232, Percent_Identity=40.0862068965517, Blast_Score=201, Evalue=1e-52,
Organism=Drosophila melanogaster, GI281364189, Length=233, Percent_Identity=38.6266094420601, Blast_Score=191, Evalue=5e-49,
Organism=Drosophila melanogaster, GI281364187, Length=233, Percent_Identity=38.6266094420601, Blast_Score=191, Evalue=5e-49,
Organism=Drosophila melanogaster, GI281364185, Length=233, Percent_Identity=38.6266094420601, Blast_Score=191, Evalue=5e-49,
Organism=Drosophila melanogaster, GI281364183, Length=233, Percent_Identity=38.6266094420601, Blast_Score=191, Evalue=5e-49,
Organism=Drosophila melanogaster, GI62471759, Length=233, Percent_Identity=38.6266094420601, Blast_Score=191, Evalue=5e-49,
Organism=Drosophila melanogaster, GI17975520, Length=233, Percent_Identity=38.6266094420601, Blast_Score=191, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_SULSO (Q980C3)

Other databases:

- EMBL:   AE006641
- PIR:   A99183
- ProteinModelPortal:   Q980C3
- SMR:   Q980C3
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0393
- NMPDR:   fig|273057.1.peg.353
- HOGENOM:   HBG397258
- OMA:   SGPTSHE
- PhylomeDB:   Q980C3
- BioCyc:   SSOL273057:SSO0393-MONOMER
- HAMAP:   MF_01081
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR004802
- InterPro:   IPR002478
- InterPro:   IPR015947
- SMART:   SM00359
- TIGRFAMs:   TIGR00425

Pfam domain/function: PF01472 PUA; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 27386; Mature: 27386

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYISKGGKEYVCVMQVHCEYNKEELAKIISSFKGEIYQRPPVRSSVKRRLRIRRIYDIE
CCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEEEEE
ILDMDKKLVLLRVNCDSGTYMRKLCHDIGIIYGCGAHMRELRRTRSGIFTESTNLVKLHE
EEECCCEEEEEEEECCCCHHHHHHHHHHCEEEECCHHHHHHHHHHCCCEECCCCEEEHHH
LSEAIYLYKNCKDETELRRVLIPMEFATCEIPKIVIEDSAVNALAYGAQLAVPGVVAYQN
HHHHHHHHHCCCCHHHHHHHHCCHHHCCCCCCEEEEECCHHHHHHHCCHHCCCCEEECCC
FKKNDTVAVLTLKGELVATGNALMDSEELENAKKGIVVNLSRVFMQRDIYPKAWKKHES
CCCCCCEEEEEECCEEEECCCHHCCHHHHHHHHCCEEEEHHHHHHHHCCCHHHHHCCCC
>Mature Secondary Structure
MNYISKGGKEYVCVMQVHCEYNKEELAKIISSFKGEIYQRPPVRSSVKRRLRIRRIYDIE
CCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEEEEE
ILDMDKKLVLLRVNCDSGTYMRKLCHDIGIIYGCGAHMRELRRTRSGIFTESTNLVKLHE
EEECCCEEEEEEEECCCCHHHHHHHHHHCEEEECCHHHHHHHHHHCCCEECCCCEEEHHH
LSEAIYLYKNCKDETELRRVLIPMEFATCEIPKIVIEDSAVNALAYGAQLAVPGVVAYQN
HHHHHHHHHCCCCHHHHHHHHCCHHHCCCCCCEEEEECCHHHHHHHCCHHCCCCEEECCC
FKKNDTVAVLTLKGELVATGNALMDSEELENAKKGIVVNLSRVFMQRDIYPKAWKKHES
CCCCCCEEEEEECCEEEECCCHHCCHHHHHHHHCCEEEEHHHHHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726