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The map label for this gene is truB [H]

Identifier: 15897326

GI number: 15897326

Start: 343263

End: 343532

Strand: Reverse

Name: truB [H]

Synonym: SSO5761

Alternate gene names: 15897326

Gene position: 343532-343263 (Counterclockwise)

Preceding gene: 15897327

Following gene: 161777478

Centisome position: 11.48

GC content: 35.19

Gene sequence:

>270_bases
ATGATTTTAAATGATTTTATTTACAAAATAGATAATTTTTGTGGATATAAAAATGAATGGAAGATAAGGAAGGATAGTGA
AACATCAGATAAATACGGATATTATCCAGAAAAAAGACCAATAGAGATACATATCAAAAACTCAATAATAAACCTTGATA
AACCTCCAGGGCCCACGAGCCATGAAGTAGCGTATTGGGTTAAAAAGATGTTAAACGTTACCAAAGCAGGACATGGGGGG
ACCCTAGAGCCCATTCATTGGGCGGGGTAA

Upstream 100 bases:

>100_bases
GAGCTTCGGATGAGGAAGTAAAGAAGAAATTAGAGGAGTCCAACTTAACAGAGTACATGAAGGAGAAAATAAAGATTAGG
ATGCCAACATTGTGATTTAG

Downstream 100 bases:

>100_bases
TCCTAAAGTTACCGGTGTGTTACCAATAGGAATAGAGAACGCAACTAAAATAATGAACTATATAAGTAAAGGAGGAAAAG
AGTACGTATGTGTAATGCAA

Product: tRNA pseudouridine synthase subunit A (truB)

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 89; Mature: 89

Protein sequence:

>89_residues
MILNDFIYKIDNFCGYKNEWKIRKDSETSDKYGYYPEKRPIEIHIKNSIINLDKPPGPTSHEVAYWVKKMLNVTKAGHGG
TLEPIHWAG

Sequences:

>Translated_89_residues
MILNDFIYKIDNFCGYKNEWKIRKDSETSDKYGYYPEKRPIEIHIKNSIINLDKPPGPTSHEVAYWVKKMLNVTKAGHGG
TLEPIHWAG
>Mature_89_residues
MILNDFIYKIDNFCGYKNEWKIRKDSETSDKYGYYPEKRPIEIHIKNSIINLDKPPGPTSHEVAYWVKKMLNVTKAGHGG
TLEPIHWAG

Specific function: Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI215599015, Length=67, Percent_Identity=43.2835820895522, Blast_Score=64, Evalue=3e-11,
Organism=Homo sapiens, GI4503337, Length=67, Percent_Identity=43.2835820895522, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17553978, Length=67, Percent_Identity=41.7910447761194, Blast_Score=63, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323204, Length=63, Percent_Identity=44.4444444444444, Blast_Score=65, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281364189, Length=67, Percent_Identity=46.2686567164179, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI281364187, Length=67, Percent_Identity=46.2686567164179, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI281364185, Length=67, Percent_Identity=46.2686567164179, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI281364183, Length=67, Percent_Identity=46.2686567164179, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI62471759, Length=67, Percent_Identity=46.2686567164179, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI17975520, Length=67, Percent_Identity=46.2686567164179, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012960
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR004802
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF08068 DKCLD; PF01472 PUA; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 10351; Mature: 10351

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILNDFIYKIDNFCGYKNEWKIRKDSETSDKYGYYPEKRPIEIHIKNSIINLDKPPGPTS
CCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCEEECCCCCCCCC
HEVAYWVKKMLNVTKAGHGGTLEPIHWAG
HHHHHHHHHHHHHHCCCCCCCCCCEECCC
>Mature Secondary Structure
MILNDFIYKIDNFCGYKNEWKIRKDSETSDKYGYYPEKRPIEIHIKNSIINLDKPPGPTS
CCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCEEECCCCCCCCC
HEVAYWVKKMLNVTKAGHGGTLEPIHWAG
HHHHHHHHHHHHHHCCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]