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The map label for this gene is trmE

Identifier: 161702952

GI number: 161702952

Start: 3688886

End: 3690280

Strand: Reverse

Name: trmE

Synonym: Bcep18194_A6518

Alternate gene names: 161702952

Gene position: 3690280-3688886 (Counterclockwise)

Preceding gene: 78067989

Following gene: 78067986

Centisome position: 99.9

GC content: 67.46

Gene sequence:

>1395_bases
ATGCTCGCTACCGATTCCGATCCGATCGTCGCCATTGCCACTGCTGCCGGCCGGGGTGGCATCGGGGTTGTCCGCGTGTC
GTTCGGGCGCGGCGGCGAGGCGGCCGCGTTGCCGCTGATCGACGCGTTGTGCGGGCAGAAGCTCGCCCCGCGCCACGCGA
GCTACGTGCCGTTCCTCGACGAGCACGGCGCGCCGCTCGATCGCGGGATCGCGCTTTACTTCCCGGCACCGCATTCGTAC
ACCGGCGAACACGTGCTCGAGCTGCAGGGGCACGGCGGGCCGATCGTGATGCAGCTGCTGCTGCAGCGTTGCCTGGATGC
GGGGCGCGGTTTCGGGCTGCGGCTAGCGGAGCCTGGCGAATTCACACGCCGCGCGTTCCTGAACGACAAGCTCGATCTTG
CACAGGCCGAGGCCGTTGCCGATCTGATCGAGGCGAGTACCGAAGCCGCCGCGCGTTCGGCCGGGCGTTCGCTCGACGGC
GCGTTCTCGCGGCAGATCCATGCGCTCGTCGACGACGTGATCACGCTGCGGATGCTGGTCGAGGCGACGCTCGATTTTCC
GGAAGAAGAGATCGATTTCCTGGAAGCGGCCGACGCGCGCGGCAAGCTCGCGAAGATCCGTGCCCAGCTCGCGCACGTGC
TGGGCGACGCGCGGCAGGGGGCGTTGCTGCGTGAAGGGCTGTCGGTCGTGCTTGCGGGGCAGCCGAACGTCGGCAAGTCG
TCGCTGCTGAATGCGCTGGCCGGCGCGGAGCTGGCGATCGTCACGCCGATTGCCGGGACGACGCGCGACAAGGTCGCGCA
GACGATCCAGGTCGAAGGGATTCCGTTGCACATCATCGATACGGCCGGGTTGCGCGAGACGGAGGACGAGGTCGAGCGGA
TCGGTATCGCGCGCACATGGAGCGAGATCGAGCGGGCGGACGTCGTGCTGCATCTGCTGGATTCGCGCAATGGGATGACG
GCGGATGACGAGACGATCGCGGCGCGGTTTCCGGGCGGCGTGCCGGTGGTGCGCGTGCTGAACAAGACAGATCTGACCGG
TGTGGCGGCGTGTGTCGAGCATCCGGCGGCGGAAGGCGACCTGACCGAGGTACATCTGTCGGCAAAGCGCGGCGACGGGA
TCGACATGCTGCGTGCGGAGTTGCTGCGGATCGCGGGGTGGCAGGCGGGGGCCGAAGGCGTGTATCTCGCGCGCGAGCGG
CATCTGATTGCGTTGAGGGCCGCGCAGGAGCATCTCGCGCAGGCGGCGGATCACGCGGAGCAGCGCGCGCAGTCACTCGA
TCTGTTTGCCGAGGAACTGCGGCTCGCGCAGGAGCAACTCAATGCGATTACCGGGGAGTTCACTTCGGATGACCTGCTGG
GGGTGATTTTCAGCAGGTTCTGTATCGGGAAGTAA

Upstream 100 bases:

>100_bases
GTGGCGCGTGTGCCGGCACGCTACAATGCCGGCGTTCCGATGCCCGAAGTGTTGCGCGGGCGCACCATATTCACTTGATC
ACCCGATTCATCCGATTCCC

Downstream 100 bases:

>100_bases
TGCCCGCGCATTTGCAGTTCGACAGCCCTTCGTGGCTCGTCATTCGTTTTTGATCGAGACATCCGATCACGATCGCACTC
CCGTTTTCGTCACGTGGCCT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MLATDSDPIVAIATAAGRGGIGVVRVSFGRGGEAAALPLIDALCGQKLAPRHASYVPFLDEHGAPLDRGIALYFPAPHSY
TGEHVLELQGHGGPIVMQLLLQRCLDAGRGFGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDG
AFSRQIHALVDDVITLRMLVEATLDFPEEEIDFLEAADARGKLAKIRAQLAHVLGDARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHIIDTAGLRETEDEVERIGIARTWSEIERADVVLHLLDSRNGMT
ADDETIAARFPGGVPVVRVLNKTDLTGVAACVEHPAAEGDLTEVHLSAKRGDGIDMLRAELLRIAGWQAGAEGVYLARER
HLIALRAAQEHLAQAADHAEQRAQSLDLFAEELRLAQEQLNAITGEFTSDDLLGVIFSRFCIGK

Sequences:

>Translated_464_residues
MLATDSDPIVAIATAAGRGGIGVVRVSFGRGGEAAALPLIDALCGQKLAPRHASYVPFLDEHGAPLDRGIALYFPAPHSY
TGEHVLELQGHGGPIVMQLLLQRCLDAGRGFGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDG
AFSRQIHALVDDVITLRMLVEATLDFPEEEIDFLEAADARGKLAKIRAQLAHVLGDARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHIIDTAGLRETEDEVERIGIARTWSEIERADVVLHLLDSRNGMT
ADDETIAARFPGGVPVVRVLNKTDLTGVAACVEHPAAEGDLTEVHLSAKRGDGIDMLRAELLRIAGWQAGAEGVYLARER
HLIALRAAQEHLAQAADHAEQRAQSLDLFAEELRLAQEQLNAITGEFTSDDLLGVIFSRFCIGK
>Mature_464_residues
MLATDSDPIVAIATAAGRGGIGVVRVSFGRGGEAAALPLIDALCGQKLAPRHASYVPFLDEHGAPLDRGIALYFPAPHSY
TGEHVLELQGHGGPIVMQLLLQRCLDAGRGFGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDG
AFSRQIHALVDDVITLRMLVEATLDFPEEEIDFLEAADARGKLAKIRAQLAHVLGDARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHIIDTAGLRETEDEVERIGIARTWSEIERADVVLHLLDSRNGMT
ADDETIAARFPGGVPVVRVLNKTDLTGVAACVEHPAAEGDLTEVHLSAKRGDGIDMLRAELLRIAGWQAGAEGVYLARER
HLIALRAAQEHLAQAADHAEQRAQSLDLFAEELRLAQEQLNAITGEFTSDDLLGVIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=476, Percent_Identity=38.2352941176471, Blast_Score=231, Evalue=9e-61,
Organism=Homo sapiens, GI193082993, Length=466, Percent_Identity=37.5536480686695, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI193082988, Length=506, Percent_Identity=35.5731225296443, Blast_Score=216, Evalue=2e-56,
Organism=Escherichia coli, GI2367268, Length=470, Percent_Identity=54.0425531914894, Blast_Score=453, Evalue=1e-128,
Organism=Escherichia coli, GI87082120, Length=161, Percent_Identity=32.9192546583851, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17507259, Length=467, Percent_Identity=29.3361884368308, Blast_Score=159, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6323665, Length=503, Percent_Identity=29.8210735586481, Blast_Score=193, Evalue=4e-50,
Organism=Drosophila melanogaster, GI45550813, Length=491, Percent_Identity=33.1975560081466, Blast_Score=187, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49453; Mature: 49453

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLATDSDPIVAIATAAGRGGIGVVRVSFGRGGEAAALPLIDALCGQKLAPRHASYVPFLD
CCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHH
EHGAPLDRGIALYFPAPHSYTGEHVLELQGHGGPIVMQLLLQRCLDAGRGFGLRLAEPGE
CCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEECCCCH
FTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDGAFSRQIHALVDDVITLRMLV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
EATLDFPEEEIDFLEAADARGKLAKIRAQLAHVLGDARQGALLREGLSVVLAGQPNVGKS
HHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHCCHHHHCCCEEEEECCCCCCHH
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHIIDTAGLRETEDEVERIGIARTW
HHHHHHCCCCEEEEECCCCCCHHHHHHEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHH
SEIERADVVLHLLDSRNGMTADDETIAARFPGGVPVVRVLNKTDLTGVAACVEHPAAEGD
HHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHEEECCCCHHHHHHHHHCCCCCCC
LTEVHLSAKRGDGIDMLRAELLRIAGWQAGAEGVYLARERHLIALRAAQEHLAQAADHAE
CEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCEEHHHHHHHHHHHHHHHHH
QRAQSLDLFAEELRLAQEQLNAITGEFTSDDLLGVIFSRFCIGK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLATDSDPIVAIATAAGRGGIGVVRVSFGRGGEAAALPLIDALCGQKLAPRHASYVPFLD
CCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHH
EHGAPLDRGIALYFPAPHSYTGEHVLELQGHGGPIVMQLLLQRCLDAGRGFGLRLAEPGE
CCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEECCCCH
FTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDGAFSRQIHALVDDVITLRMLV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
EATLDFPEEEIDFLEAADARGKLAKIRAQLAHVLGDARQGALLREGLSVVLAGQPNVGKS
HHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHCCHHHHCCCEEEEECCCCCCHH
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHIIDTAGLRETEDEVERIGIARTW
HHHHHHCCCCEEEEECCCCCCHHHHHHEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHH
SEIERADVVLHLLDSRNGMTADDETIAARFPGGVPVVRVLNKTDLTGVAACVEHPAAEGD
HHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHEEECCCCHHHHHHHHHCCCCCCC
LTEVHLSAKRGDGIDMLRAELLRIAGWQAGAEGVYLARERHLIALRAAQEHLAQAADHAE
CEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCEEHHHHHHHHHHHHHHHHH
QRAQSLDLFAEELRLAQEQLNAITGEFTSDDLLGVIFSRFCIGK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA