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The map label for this gene is dctA1

Identifier: 161621446

GI number: 161621446

Start: 4714062

End: 4715495

Strand: Reverse

Name: dctA1

Synonym: mll5840

Alternate gene names: 161621446

Gene position: 4715495-4714062 (Counterclockwise)

Preceding gene: 13474868

Following gene: 13474863

Centisome position: 67.02

GC content: 58.23

Gene sequence:

>1434_bases
ATGTTGACCCCACTGGCTCCAAGCGTAAAGCGCGCACCCCGCAAGACCCTTTTTGCCAAGCCTTACGTGCAGGTGCTTGT
CGCAATCCTGCTCGGAGTTGCGGTTGGCCATTTTTATCCGCAAATCGGAGAAAACCTGAAGCCGCTCGGCGATGCCTTCA
TCAAGCTCGTCAAGATGATCATCGCGCCCGTTATCTTTCTGACCGTGTCGACTGGAATTGCCGGCATGAGCGATCTTCAG
AAGGTCGGCCGTGTTGCCGGCAAGGCGATGGTCTATTTCGTCACCTTCTCGACGCTCGCGCTTATCGTCGGCCTCATCGT
CGGCAATGTCATTCAGCCTGGCGCCGGCCTCAACATCGATCTGACCTCGCTCGACGTCCAAGCCGTCAATGGCTATGCCT
CAAAGGCCCATGAGCAGTCCGTGACCGGCTTCCTCATGAACATGATCCCGACCACGATTGTCGGCGCCTTTGTGGAAGGC
GACATTCTACAGGTGTTGTTCTTCTCCGTCCTCTTCGGCATCGCGCTGGCGATGGTCGGAGAATCGGGCAAATCGGTTCT
TTCCTTCCTTCAGGACCTCACTGCACCCGTTTTCAAATTGGTCGGCATCCTGATGAAAGCCGCGCCGATCGGTGCTTTCG
GAGCAATGGCCTTCACGATTGGCAAATATGGCATCGGTTCGGTAGCCAACCTTGCGATGCTGGTCGGGACCTTCTATCTC
ACGGCCTTCCTCTTCGTATTCGGGGTTCTCGGTGCAGTCTGTCGCTACAATGGCTTCTCGATCTTTTCTCTTATCCGCTA
CATAAAGGAAGAGCTGCTGCTGGTCCTCGGAACGTCCTCCTCCGAGGCTGCGCTGCCCTCTCTCATGGAGAAAATGGAAA
AGGCCGGCGCCAAGCGCTCGGTCGTGGGCCTGGTTATCCCGACCGGATATTCCTTCAATCTGGATGGCACCAATATCTAC
ATGACCCTCGCGGCCCTTTTCATCGCGCAGGCGACGAATACGGATTTGTCAGTTGGCGATCAGGTCCTGCTGCTGCTCGT
CGCGATGCTTTCCTCCAAGGGTGCAGCAGGTGTCACAGGTGCCGGCTTCGTCACGTTGGCTGCTACGCTCTCCGTAGTGC
CGGCAGTTCCCGTTGCTGGAATGGCGCTGATCCTTGGCGTCGATCGCTTCATGTCGGAATGCAGGGCACTGACGAATTTG
GTCGGTAACGCGGTGGCATCGCTCGTCGTCGCCCGCTGGGAAGGCGAACTGGATCAGGCGCAATTGAAAGCTGCATTCTG
CGGCCACCAGCCTGCCGAGACATCAGCCGGCCAGCCACTGATAACGCCCGCGCCGAGCAACTCGGCAGCATCGCTGCCGG
TTGAATCGCCTGGCTGGTCCCAAACGCCGGACGATCGGGCCGCGGGTTCCAAACAAACTCTCGCGGGCCGATGA

Upstream 100 bases:

>100_bases
TGCGGTCAACAACGCAGCAGCGTTCGACCGGGGCGCCGCGATGGGAGAAGCCGCGGGGAGGAACAGCCCGCCCCAAACCG
GTCAGGGAGAGAAAACTTAC

Downstream 100 bases:

>100_bases
AATCAACAATCCCGAAATGCAGTGGGTGCAAAATCATCTGCGCCTTGCGAAGGTCTTGCACCGGTCACGACACACAAGCC
CTCGATGATGCGCTTAATGC

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MLTPLAPSVKRAPRKTLFAKPYVQVLVAILLGVAVGHFYPQIGENLKPLGDAFIKLVKMIIAPVIFLTVSTGIAGMSDLQ
KVGRVAGKAMVYFVTFSTLALIVGLIVGNVIQPGAGLNIDLTSLDVQAVNGYASKAHEQSVTGFLMNMIPTTIVGAFVEG
DILQVLFFSVLFGIALAMVGESGKSVLSFLQDLTAPVFKLVGILMKAAPIGAFGAMAFTIGKYGIGSVANLAMLVGTFYL
TAFLFVFGVLGAVCRYNGFSIFSLIRYIKEELLLVLGTSSSEAALPSLMEKMEKAGAKRSVVGLVIPTGYSFNLDGTNIY
MTLAALFIAQATNTDLSVGDQVLLLLVAMLSSKGAAGVTGAGFVTLAATLSVVPAVPVAGMALILGVDRFMSECRALTNL
VGNAVASLVVARWEGELDQAQLKAAFCGHQPAETSAGQPLITPAPSNSAASLPVESPGWSQTPDDRAAGSKQTLAGR

Sequences:

>Translated_477_residues
MLTPLAPSVKRAPRKTLFAKPYVQVLVAILLGVAVGHFYPQIGENLKPLGDAFIKLVKMIIAPVIFLTVSTGIAGMSDLQ
KVGRVAGKAMVYFVTFSTLALIVGLIVGNVIQPGAGLNIDLTSLDVQAVNGYASKAHEQSVTGFLMNMIPTTIVGAFVEG
DILQVLFFSVLFGIALAMVGESGKSVLSFLQDLTAPVFKLVGILMKAAPIGAFGAMAFTIGKYGIGSVANLAMLVGTFYL
TAFLFVFGVLGAVCRYNGFSIFSLIRYIKEELLLVLGTSSSEAALPSLMEKMEKAGAKRSVVGLVIPTGYSFNLDGTNIY
MTLAALFIAQATNTDLSVGDQVLLLLVAMLSSKGAAGVTGAGFVTLAATLSVVPAVPVAGMALILGVDRFMSECRALTNL
VGNAVASLVVARWEGELDQAQLKAAFCGHQPAETSAGQPLITPAPSNSAASLPVESPGWSQTPDDRAAGSKQTLAGR
>Mature_477_residues
MLTPLAPSVKRAPRKTLFAKPYVQVLVAILLGVAVGHFYPQIGENLKPLGDAFIKLVKMIIAPVIFLTVSTGIAGMSDLQ
KVGRVAGKAMVYFVTFSTLALIVGLIVGNVIQPGAGLNIDLTSLDVQAVNGYASKAHEQSVTGFLMNMIPTTIVGAFVEG
DILQVLFFSVLFGIALAMVGESGKSVLSFLQDLTAPVFKLVGILMKAAPIGAFGAMAFTIGKYGIGSVANLAMLVGTFYL
TAFLFVFGVLGAVCRYNGFSIFSLIRYIKEELLLVLGTSSSEAALPSLMEKMEKAGAKRSVVGLVIPTGYSFNLDGTNIY
MTLAALFIAQATNTDLSVGDQVLLLLVAMLSSKGAAGVTGAGFVTLAATLSVVPAVPVAGMALILGVDRFMSECRALTNL
VGNAVASLVVARWEGELDQAQLKAAFCGHQPAETSAGQPLITPAPSNSAASLPVESPGWSQTPDDRAAGSKQTLAGR

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane. This transport system plays an important role in the energy supply of rhizobium-legume symbionts

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family

Homologues:

Organism=Homo sapiens, GI40254478, Length=462, Percent_Identity=26.1904761904762, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI21314632, Length=461, Percent_Identity=26.6811279826464, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI4827012, Length=274, Percent_Identity=32.4817518248175, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI169790839, Length=433, Percent_Identity=25.8660508083141, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI194239697, Length=288, Percent_Identity=31.25, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI66773030, Length=480, Percent_Identity=25.625, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI223468566, Length=326, Percent_Identity=29.4478527607362, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI5032093, Length=428, Percent_Identity=27.1028037383178, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI223468564, Length=296, Percent_Identity=29.0540540540541, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI301601644, Length=218, Percent_Identity=30.2752293577982, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI262359914, Length=376, Percent_Identity=25, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1789947, Length=418, Percent_Identity=58.3732057416268, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI1790514, Length=412, Percent_Identity=39.0776699029126, Blast_Score=307, Evalue=1e-84,
Organism=Escherichia coli, GI1788024, Length=395, Percent_Identity=23.0379746835443, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789473, Length=226, Percent_Identity=26.9911504424779, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17537407, Length=430, Percent_Identity=26.5116279069767, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71996953, Length=442, Percent_Identity=26.9230769230769, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI71983106, Length=457, Percent_Identity=25.382932166302, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71983099, Length=457, Percent_Identity=25.382932166302, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI193206505, Length=421, Percent_Identity=24.4655581947743, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI193206654, Length=438, Percent_Identity=25.3424657534247, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17541374, Length=397, Percent_Identity=25.4408060453401, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24583025, Length=398, Percent_Identity=24.8743718592965, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17137668, Length=398, Percent_Identity=24.8743718592965, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24583023, Length=398, Percent_Identity=24.8743718592965, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI281360483, Length=452, Percent_Identity=26.3274336283186, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17137666, Length=452, Percent_Identity=26.3274336283186, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281360481, Length=452, Percent_Identity=26.3274336283186, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCTA1_RHILO (Q98AV2)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_106434.2
- ProteinModelPortal:   Q98AV2
- SMR:   Q98AV2
- GeneID:   1229093
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll5840
- NMPDR:   fig|266835.1.peg.4537
- HOGENOM:   HBG739804
- ProtClustDB:   PRK13027
- HAMAP:   MF_01300
- InterPro:   IPR001991
- InterPro:   IPR018107
- PANTHER:   PTHR11958
- PRINTS:   PR00173

Pfam domain/function: PF00375 SDF

EC number: NA

Molecular weight: Translated: 49646; Mature: 49646

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1; PS00714 NA_DICARBOXYL_SYMP_2; PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1481b274)-; HASH(0x11e3b754)-; HASH(0x1493c0f4)-; HASH(0x15000d70)-; HASH(0x14da0038)-; HASH(0x157e8c24)-; HASH(0x13d52164)-; HASH(0x1543e308)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTPLAPSVKRAPRKTLFAKPYVQVLVAILLGVAVGHFYPQIGENLKPLGDAFIKLVKMI
CCCCCCCHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
IAPVIFLTVSTGIAGMSDLQKVGRVAGKAMVYFVTFSTLALIVGLIVGNVIQPGAGLNID
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
LTSLDVQAVNGYASKAHEQSVTGFLMNMIPTTIVGAFVEGDILQVLFFSVLFGIALAMVG
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ESGKSVLSFLQDLTAPVFKLVGILMKAAPIGAFGAMAFTIGKYGIGSVANLAMLVGTFYL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
TAFLFVFGVLGAVCRYNGFSIFSLIRYIKEELLLVLGTSSSEAALPSLMEKMEKAGAKRS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCCCC
VVGLVIPTGYSFNLDGTNIYMTLAALFIAQATNTDLSVGDQVLLLLVAMLSSKGAAGVTG
EEEEEEECCEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
AGFVTLAATLSVVPAVPVAGMALILGVDRFMSECRALTNLVGNAVASLVVARWEGELDQA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
QLKAAFCGHQPAETSAGQPLITPAPSNSAASLPVESPGWSQTPDDRAAGSKQTLAGR
HHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCH
>Mature Secondary Structure
MLTPLAPSVKRAPRKTLFAKPYVQVLVAILLGVAVGHFYPQIGENLKPLGDAFIKLVKMI
CCCCCCCHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
IAPVIFLTVSTGIAGMSDLQKVGRVAGKAMVYFVTFSTLALIVGLIVGNVIQPGAGLNID
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
LTSLDVQAVNGYASKAHEQSVTGFLMNMIPTTIVGAFVEGDILQVLFFSVLFGIALAMVG
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ESGKSVLSFLQDLTAPVFKLVGILMKAAPIGAFGAMAFTIGKYGIGSVANLAMLVGTFYL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
TAFLFVFGVLGAVCRYNGFSIFSLIRYIKEELLLVLGTSSSEAALPSLMEKMEKAGAKRS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCCCC
VVGLVIPTGYSFNLDGTNIYMTLAALFIAQATNTDLSVGDQVLLLLVAMLSSKGAAGVTG
EEEEEEECCEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
AGFVTLAATLSVVPAVPVAGMALILGVDRFMSECRALTNLVGNAVASLVVARWEGELDQA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
QLKAAFCGHQPAETSAGQPLITPAPSNSAASLPVESPGWSQTPDDRAAGSKQTLAGR
HHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11214968