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The map label for this gene is rumA

Identifier: 161621317

GI number: 161621317

Start: 2311881

End: 2313299

Strand: Direct

Name: rumA

Synonym: Bxe_A2348

Alternate gene names: 161621317

Gene position: 2311881-2313299 (Clockwise)

Preceding gene: 91783467

Following gene: 91783470

Centisome position: 47.22

GC content: 63.99

Gene sequence:

>1419_bases
GTGTCCCGAACTGCCCCCCATCGCCGCGCGCCGAAGCGCTACAAGACGCCTCCGCCCGCGCCGGCACACGTCGTCACCGG
CAATGAGCCGGTCATCGAAATCATTTCGCTCGATATGGAAGCGCGCGGCGTCGGCCGCACCGAGACCGAAGACGGCACAC
CCGGCAAGGTGATCTTCGTCGAAGGTGTGCTGCCCGGTGAACGCGTGAGTTATTCGACGCATCGCAGCAAGCCGAAATTC
GAGCAGGCCGAAGTGGTGCAGGTATTGCGCGAAAGCGTGATGCGCACCAAGCCGAAGTGCACTTACTTCGATATCTGCGG
CGGCTGTTCGATGCAGCATCTGGACATTCGCGCGCAGGTAGCGGTCAAGCAGCGCGTGCTCGAAGACAACCTCCAGCATC
TGGCGAAGCTGCGCCCGGAAACGGTGTTCCGGCCGATTCACGGGCCCTCCTGGGGTTATCGCTACCGCGCGCGTCTGGCC
GTGCGTTTCCTGCCGGAAAAGGGCGGTATGCGGATCGGCTTCCACGAGAAGAAGAGCAGCTACATCGCCGACATGAAGAC
CTGCGAAGTGCTGCCGCCGCATGTGTCGGCCATGCTGATGCCGCTGCGCTTCATGGTCCGCAAGCTGTCGATTTACGACC
GTATGCCGCAGCTCGAACTGGCGGTCGGATCATCGGTGACGGCGCTGGTGGTGCGTAATCTCGAACCCATCACTGCGGCC
GACGAGCAGGTGCTGCGCGATTTCGCCGACGAGCACAAGGTGCAGTTCTGGCTGCAGCCGGGCGGCCCGGATACGGTCAC
GCCGTTCTATCCGCTCGACGTGCAGCTCGACTACACGTTGCCGGAGTACGGCATCCGCATGCCGTTCAAACCAACCGATT
TCACCCAGGTGAATCACGCCATCAACCGCGTGCTGGTGAGCCGCGCGCTGCGGCTGCTGGCGCCGGCGCGTACCGATCGC
GTGCTCGATCTGTTCTGCGGGATCGGCAACTTCACTTTGCCGCTCGCGCGAATCTCGAAAGAAGTGATGGGTATCGAAGG
CAGCGAGGTGCTGACTTCGCGTGCGCTCGCCAATGCCGGATTGAATGGCGTGGCGGGGCATACGTCGTTTGAATGCCGCA
ACCTGTTCGAAGTCACCGCCGACGACCTGCGCGCGCTCGGCCACTTTGACAAGTTCCTCATCGACCCGCCGCGCGAAGGC
GCGCTGGCCGTCGCCAAGGCGCTCGCGGAAATTGCCCGCAGCGGCAATGGCCCGCTGCCGAAGCGCATCGTCTACGTGTC
ATGCGCGCCGGCGACGCTCGCTCGCGACGCCGGTCTGCTCGTGCATGAGGCCGGCTATCGCCTCGTGGGTGCGGGCGTGG
TGAACATGTTCCCGCACACGTCGCATGTGGAGTCGATCGCGTTGTTCGAGCGGGACTGA

Upstream 100 bases:

>100_bases
TCGCGCGGCTGGCGGCCATTCCCGCCGCGCCGAATGATGGTGAGGCGCGGCGCTTCGTCTACAATCTCGCCTTTCCCCCA
AGTTTGTCAGGAAGTAGCAG

Downstream 100 bases:

>100_bases
ACAGCGCCGCCGCCGTGCCCGGTGCGGCATGGCAGCGCGGCAGCGTAGCGCGGGGCACAAACAGAAACGCCACGACCGAA
GTCGTGGCGTTTTCTATTGC

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSRTAPHRRAPKRYKTPPPAPAHVVTGNEPVIEIISLDMEARGVGRTETEDGTPGKVIFVEGVLPGERVSYSTHRSKPKF
EQAEVVQVLRESVMRTKPKCTYFDICGGCSMQHLDIRAQVAVKQRVLEDNLQHLAKLRPETVFRPIHGPSWGYRYRARLA
VRFLPEKGGMRIGFHEKKSSYIADMKTCEVLPPHVSAMLMPLRFMVRKLSIYDRMPQLELAVGSSVTALVVRNLEPITAA
DEQVLRDFADEHKVQFWLQPGGPDTVTPFYPLDVQLDYTLPEYGIRMPFKPTDFTQVNHAINRVLVSRALRLLAPARTDR
VLDLFCGIGNFTLPLARISKEVMGIEGSEVLTSRALANAGLNGVAGHTSFECRNLFEVTADDLRALGHFDKFLIDPPREG
ALAVAKALAEIARSGNGPLPKRIVYVSCAPATLARDAGLLVHEAGYRLVGAGVVNMFPHTSHVESIALFERD

Sequences:

>Translated_472_residues
MSRTAPHRRAPKRYKTPPPAPAHVVTGNEPVIEIISLDMEARGVGRTETEDGTPGKVIFVEGVLPGERVSYSTHRSKPKF
EQAEVVQVLRESVMRTKPKCTYFDICGGCSMQHLDIRAQVAVKQRVLEDNLQHLAKLRPETVFRPIHGPSWGYRYRARLA
VRFLPEKGGMRIGFHEKKSSYIADMKTCEVLPPHVSAMLMPLRFMVRKLSIYDRMPQLELAVGSSVTALVVRNLEPITAA
DEQVLRDFADEHKVQFWLQPGGPDTVTPFYPLDVQLDYTLPEYGIRMPFKPTDFTQVNHAINRVLVSRALRLLAPARTDR
VLDLFCGIGNFTLPLARISKEVMGIEGSEVLTSRALANAGLNGVAGHTSFECRNLFEVTADDLRALGHFDKFLIDPPREG
ALAVAKALAEIARSGNGPLPKRIVYVSCAPATLARDAGLLVHEAGYRLVGAGVVNMFPHTSHVESIALFERD
>Mature_471_residues
SRTAPHRRAPKRYKTPPPAPAHVVTGNEPVIEIISLDMEARGVGRTETEDGTPGKVIFVEGVLPGERVSYSTHRSKPKFE
QAEVVQVLRESVMRTKPKCTYFDICGGCSMQHLDIRAQVAVKQRVLEDNLQHLAKLRPETVFRPIHGPSWGYRYRARLAV
RFLPEKGGMRIGFHEKKSSYIADMKTCEVLPPHVSAMLMPLRFMVRKLSIYDRMPQLELAVGSSVTALVVRNLEPITAAD
EQVLRDFADEHKVQFWLQPGGPDTVTPFYPLDVQLDYTLPEYGIRMPFKPTDFTQVNHAINRVLVSRALRLLAPARTDRV
LDLFCGIGNFTLPLARISKEVMGIEGSEVLTSRALANAGLNGVAGHTSFECRNLFEVTADDLRALGHFDKFLIDPPREGA
LAVAKALAEIARSGNGPLPKRIVYVSCAPATLARDAGLLVHEAGYRLVGAGVVNMFPHTSHVESIALFERD

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Escherichia coli, GI1789148, Length=446, Percent_Identity=36.7713004484305, Blast_Score=275, Evalue=3e-75,
Organism=Escherichia coli, GI1787083, Length=185, Percent_Identity=29.7297297297297, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24666579, Length=211, Percent_Identity=31.7535545023697, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMA_BURXL (Q13Z67)

Other databases:

- EMBL:   CP000270
- RefSeq:   YP_558674.2
- ProteinModelPortal:   Q13Z67
- GeneID:   4003741
- GenomeReviews:   CP000270_GR
- KEGG:   bxe:Bxe_A2348
- HOGENOM:   HBG690163
- OMA:   HRADGKV
- ProtClustDB:   PRK13168
- HAMAP:   MF_01010
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: =2.1.1.190

Molecular weight: Translated: 52414; Mature: 52283

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 428-428 BINDING 296-296 BINDING 325-325 BINDING 330-330 BINDING 346-346 BINDING 374-374 BINDING 395-395

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTAPHRRAPKRYKTPPPAPAHVVTGNEPVIEIISLDMEARGVGRTETEDGTPGKVIFV
CCCCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEEECHHHCCCCCCCCCCCCCCEEEEE
EGVLPGERVSYSTHRSKPKFEQAEVVQVLRESVMRTKPKCTYFDICGGCSMQHLDIRAQV
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEHHHHHHH
AVKQRVLEDNLQHLAKLRPETVFRPIHGPSWGYRYRARLAVRFLPEKGGMRIGFHEKKSS
HHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCEEEEEECCCHH
YIADMKTCEVLPPHVSAMLMPLRFMVRKLSIYDRMPQLELAVGSSVTALVVRNLEPITAA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEHHHHCCCCCCCH
DEQVLRDFADEHKVQFWLQPGGPDTVTPFYPLDVQLDYTLPEYGIRMPFKPTDFTQVNHA
HHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHH
INRVLVSRALRLLAPARTDRVLDLFCGIGNFTLPLARISKEVMGIEGSEVLTSRALANAG
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
LNGVAGHTSFECRNLFEVTADDLRALGHFDKFLIDPPREGALAVAKALAEIARSGNGPLP
CCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCC
KRIVYVSCAPATLARDAGLLVHEAGYRLVGAGVVNMFPHTSHVESIALFERD
CEEEEEEECCHHHHHCCCEEEECCCCEEEECCHHHHCCCCHHHHHEEEEECC
>Mature Secondary Structure 
SRTAPHRRAPKRYKTPPPAPAHVVTGNEPVIEIISLDMEARGVGRTETEDGTPGKVIFV
CCCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEEECHHHCCCCCCCCCCCCCCEEEEE
EGVLPGERVSYSTHRSKPKFEQAEVVQVLRESVMRTKPKCTYFDICGGCSMQHLDIRAQV
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEHHHHHHH
AVKQRVLEDNLQHLAKLRPETVFRPIHGPSWGYRYRARLAVRFLPEKGGMRIGFHEKKSS
HHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCEEEEEECCCHH
YIADMKTCEVLPPHVSAMLMPLRFMVRKLSIYDRMPQLELAVGSSVTALVVRNLEPITAA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEHHHHCCCCCCCH
DEQVLRDFADEHKVQFWLQPGGPDTVTPFYPLDVQLDYTLPEYGIRMPFKPTDFTQVNHA
HHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHH
INRVLVSRALRLLAPARTDRVLDLFCGIGNFTLPLARISKEVMGIEGSEVLTSRALANAG
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
LNGVAGHTSFECRNLFEVTADDLRALGHFDKFLIDPPREGALAVAKALAEIARSGNGPLP
CCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCC
KRIVYVSCAPATLARDAGLLVHEAGYRLVGAGVVNMFPHTSHVESIALFERD
CEEEEEEECCHHHHHCCCEEEECCCCEEEECCHHHHCCCCHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA