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The map label for this gene is mdh

Identifier: 161611326

GI number: 161611326

Start: 880705

End: 881712

Strand: Direct

Name: mdh

Synonym: Bd0928

Alternate gene names: 161611326

Gene position: 880705-881712 (Clockwise)

Preceding gene: 42522495

Following gene: 42522499

Centisome position: 23.28

GC content: 49.5

Gene sequence:

>1008_bases
ATGAAAGCTCCTGTTCGTGTAGCAGTTACCGGCGCTGCCGGCCAAATCGGTTATGCACTTCTTTTCCGTATCGCCAGCGG
TGCGATGTTGGGTGCAGACCAACCAGTGATCCTTCAACTTCTAGAGATCCCAGACGAAAAAGCTCAAAAAGCGCTAAAGG
GCGTGATGATGGAGCTTGAGGACTGCGCGTTCCCTCTATTGCACTCTATGATCGCTACGGGTGATCCTGCAGTTGCATTC
AAAGACGCTGATGTGGCCCTTCTAGTTGGTGCACGTCCTCGTGGCCCAGGTATGGAGCGTAAAGATCTTCTGACTGCAAA
CGGTCAGATCTTCACTGTACAAGGTGAAGCGATCGGTAAATACGCTAATCCAAATGTAAAAGTTTTGGTTGTTGGTAACC
CAGCAAACACAAACGCCTACATCGCGATGAAATCTGCGATGAAACACGGCCGCGTAAAAGCGAAAAACTTCACAGCAATG
CTTCGTTTGGACCACAACCGTGCATTGTCTCAGTTGGCGACCAAAACCGGCAAACCAGTTGCTTCTTTCAAAAAAGTGGC
AGTATGGGGTAACCACAGCCCAACGATGTACCCGGATGTTCGTTTCGCAACAGCTGATGGCGCAAAAGTTCCTGAGTTGT
TGAAACTGGGCACGGCTGAAGGTGATGCTTGGAACAAAGACACGTTCATCCCGACAGTGGGTAAACGTGGTGCTGCGATC
ATCGAGGCTCGTGGTTTGTCTTCTGCAGCTTCTGCGGCTTCCGCAGCTGTGGATCACATCCGTGACTGGTGGTTGGGCAC
AAATGGCGAGTGGGTTACTATGGGTATCCCTTCTGATGGTTCTTATGACATCCCAGAAGGCATCATGTACGGCTTCCCAG
TGACTTGCAAAAACGGCGAATACGAGATCGTAAAAGGTCTTGAGATCGACGCATTCTCTCGCGAGAAAATGAACAACACA
CTGAAAGAATTGAACGAAGAAAAAGACGCTGTTGCTTCTATGCTTTAA

Upstream 100 bases:

>100_bases
AAAACAGGCATATTTTCCCGATTTTCCCATGACAGAGGCTTTCAGCGGGTTTAAATTCTTCGGGATAGCTAAATCGACAA
TCTTTGAAAGGAACCAGCTT

Downstream 100 bases:

>100_bases
TTCTTAATTCAAATATTGAATTGAAAAAGGCCGGTGAAAACCGGCCTTTTTTTATTCTTAATCAGAGTACTGGGGTTCGA
CTCTTTTATTTTCCGAAGTT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKAPVRVAVTGAAGQIGYALLFRIASGAMLGADQPVILQLLEIPDEKAQKALKGVMMELEDCAFPLLHSMIATGDPAVAF
KDADVALLVGARPRGPGMERKDLLTANGQIFTVQGEAIGKYANPNVKVLVVGNPANTNAYIAMKSAMKHGRVKAKNFTAM
LRLDHNRALSQLATKTGKPVASFKKVAVWGNHSPTMYPDVRFATADGAKVPELLKLGTAEGDAWNKDTFIPTVGKRGAAI
IEARGLSSAASAASAAVDHIRDWWLGTNGEWVTMGIPSDGSYDIPEGIMYGFPVTCKNGEYEIVKGLEIDAFSREKMNNT
LKELNEEKDAVASML

Sequences:

>Translated_335_residues
MKAPVRVAVTGAAGQIGYALLFRIASGAMLGADQPVILQLLEIPDEKAQKALKGVMMELEDCAFPLLHSMIATGDPAVAF
KDADVALLVGARPRGPGMERKDLLTANGQIFTVQGEAIGKYANPNVKVLVVGNPANTNAYIAMKSAMKHGRVKAKNFTAM
LRLDHNRALSQLATKTGKPVASFKKVAVWGNHSPTMYPDVRFATADGAKVPELLKLGTAEGDAWNKDTFIPTVGKRGAAI
IEARGLSSAASAASAAVDHIRDWWLGTNGEWVTMGIPSDGSYDIPEGIMYGFPVTCKNGEYEIVKGLEIDAFSREKMNNT
LKELNEEKDAVASML
>Mature_335_residues
MKAPVRVAVTGAAGQIGYALLFRIASGAMLGADQPVILQLLEIPDEKAQKALKGVMMELEDCAFPLLHSMIATGDPAVAF
KDADVALLVGARPRGPGMERKDLLTANGQIFTVQGEAIGKYANPNVKVLVVGNPANTNAYIAMKSAMKHGRVKAKNFTAM
LRLDHNRALSQLATKTGKPVASFKKVAVWGNHSPTMYPDVRFATADGAKVPELLKLGTAEGDAWNKDTFIPTVGKRGAAI
IEARGLSSAASAASAAVDHIRDWWLGTNGEWVTMGIPSDGSYDIPEGIMYGFPVTCKNGEYEIVKGLEIDAFSREKMNNT
LKELNEEKDAVASML

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=341, Percent_Identity=53.0791788856305, Blast_Score=334, Evalue=7e-92,
Organism=Homo sapiens, GI89886456, Length=338, Percent_Identity=25.1479289940828, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17561064, Length=332, Percent_Identity=54.2168674698795, Blast_Score=334, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI32566798, Length=269, Percent_Identity=55.0185873605948, Blast_Score=265, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI32566800, Length=173, Percent_Identity=52.0231213872832, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24583394, Length=338, Percent_Identity=52.3668639053254, Blast_Score=319, Evalue=1e-87,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_BDEBA (P61973)

Other databases:

- EMBL:   BX842648
- RefSeq:   NP_967876.2
- ProteinModelPortal:   P61973
- SMR:   P61973
- GeneID:   2733201
- GenomeReviews:   BX842601_GR
- KEGG:   bba:Bd0928
- NMPDR:   fig|264462.1.peg.846
- HOGENOM:   HBG289884
- ProtClustDB:   PRK05442
- BioCyc:   BBAC264462:BD0928-MONOMER
- BRENDA:   1.1.1.37
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 35800; Mature: 35800

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 192-192 BINDING 94-94 BINDING 100-100 BINDING 107-107 BINDING 114-114 BINDING 133-133 BINDING 167-167

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAPVRVAVTGAAGQIGYALLFRIASGAMLGADQPVILQLLEIPDEKAQKALKGVMMELE
CCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
DCAFPLLHSMIATGDPAVAFKDADVALLVGARPRGPGMERKDLLTANGQIFTVQGEAIGK
HHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCCCCCHHHEEECCCEEEEEECHHCCC
YANPNVKVLVVGNPANTNAYIAMKSAMKHGRVKAKNFTAMLRLDHNRALSQLATKTGKPV
CCCCCEEEEEEECCCCCCEEEEEHHHHHCCCEEECCEEEEEEECCHHHHHHHHHHCCCCH
ASFKKVAVWGNHSPTMYPDVRFATADGAKVPELLKLGTAEGDAWNKDTFIPTVGKRGAAI
HHHEEEEEECCCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEE
IEARGLSSAASAASAAVDHIRDWWLGTNGEWVTMGIPSDGSYDIPEGIMYGFPVTCKNGE
EEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEECCEEEECCCC
YEIVKGLEIDAFSREKMNNTLKELNEEKDAVASML
EEEEECCEECCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKAPVRVAVTGAAGQIGYALLFRIASGAMLGADQPVILQLLEIPDEKAQKALKGVMMELE
CCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
DCAFPLLHSMIATGDPAVAFKDADVALLVGARPRGPGMERKDLLTANGQIFTVQGEAIGK
HHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCCCCCHHHEEECCCEEEEEECHHCCC
YANPNVKVLVVGNPANTNAYIAMKSAMKHGRVKAKNFTAMLRLDHNRALSQLATKTGKPV
CCCCCEEEEEEECCCCCCEEEEEHHHHHCCCEEECCEEEEEEECCHHHHHHHHHHCCCCH
ASFKKVAVWGNHSPTMYPDVRFATADGAKVPELLKLGTAEGDAWNKDTFIPTVGKRGAAI
HHHEEEEEECCCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEE
IEARGLSSAASAASAAVDHIRDWWLGTNGEWVTMGIPSDGSYDIPEGIMYGFPVTCKNGE
EEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEECCEEEECCCC
YEIVKGLEIDAFSREKMNNTLKELNEEKDAVASML
EEEEECCEECCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA