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The map label for this gene is ubiE [H]

Identifier: 161598678

GI number: 161598678

Start: 390814

End: 391503

Strand: Direct

Name: ubiE [H]

Synonym: DIP0426

Alternate gene names: 161598678

Gene position: 390814-391503 (Clockwise)

Preceding gene: 38233039

Following gene: 38233043

Centisome position: 15.7

GC content: 53.91

Gene sequence:

>690_bases
GTGGCAAAGGCATCATTGGATAAGAAGCCCCTCGACGTTGCAAAAATGTTCGACGGCGTGGGCAAGAACTACGACATCAC
CAATACGGTGTTGAGTTTTGGGCGCGATAAAAAGTGGCGGGAAAAGACCCGTGAGCGTCTCCAGCTCAAAGCGGGTGAAC
GCGTGCTCGACCTTGCGGCAGGAACAGCGGTGTCTACCGTGGAACTCACCCGATCTGGTGCATGGGTAGTTGCGTGCGAT
TTCTCCCAAGGCATGCTGGCAGCCGGTAAGGATCGCAATGTTCCCAAGGTTGTGGGCGACGGCATGAAGCTGCCCTTTGC
TGACAACTCCTTTGACGCTGTCACCATTTCTTATGGTTTGCGCAACGTTCATGATTTCCGTATGGCTTTGCGCGAGATGG
CGCGAGTAACCAAGCCTGGTGGTCGTATTACTATCGCGGAGTTTTCCACCCCGAACCTACCTGGTTTTAAAACCATCTAC
AAGGAATATCTCACTAAGCTACTGCCATTAATCGCCAAGGTTGTAGGCACCAACCCAGAGGCTTATGAGTATTTGGCGGA
ATCGATCCGCGCATGGCCGGAGCGAGAAGAACTCGCCGCGGCCATCAATGAAAACGGCTGGCACGATGCCGGCTGGCAAA
ATCTCAGCCTCGGCATCGTGGCTCTTCACTCGGCTGTTAAGCCTTCCTAA

Upstream 100 bases:

>100_bases
CTTGCGCATGACTTTGATCCGCGGTCGACAGTGGTGACGTGGGAGACTGTCTGTAGCCAGCTCTTCGCCCATTATCGTGA
TGTTGTTACACTGGACAACC

Downstream 100 bases:

>100_bases
CAGTCTCGCGGATAAATTCTGCGGACTTATGCAGCTGCTCTAGCTCCCATTCGTTGAGCTGGGGCAGCATTTTGCGTCCA
GCACCTTCGTGGGTGATCTC

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MAKASLDKKPLDVAKMFDGVGKNYDITNTVLSFGRDKKWREKTRERLQLKAGERVLDLAAGTAVSTVELTRSGAWVVACD
FSQGMLAAGKDRNVPKVVGDGMKLPFADNSFDAVTISYGLRNVHDFRMALREMARVTKPGGRITIAEFSTPNLPGFKTIY
KEYLTKLLPLIAKVVGTNPEAYEYLAESIRAWPEREELAAAINENGWHDAGWQNLSLGIVALHSAVKPS

Sequences:

>Translated_229_residues
MAKASLDKKPLDVAKMFDGVGKNYDITNTVLSFGRDKKWREKTRERLQLKAGERVLDLAAGTAVSTVELTRSGAWVVACD
FSQGMLAAGKDRNVPKVVGDGMKLPFADNSFDAVTISYGLRNVHDFRMALREMARVTKPGGRITIAEFSTPNLPGFKTIY
KEYLTKLLPLIAKVVGTNPEAYEYLAESIRAWPEREELAAAINENGWHDAGWQNLSLGIVALHSAVKPS
>Mature_228_residues
AKASLDKKPLDVAKMFDGVGKNYDITNTVLSFGRDKKWREKTRERLQLKAGERVLDLAAGTAVSTVELTRSGAWVVACDF
SQGMLAAGKDRNVPKVVGDGMKLPFADNSFDAVTISYGLRNVHDFRMALREMARVTKPGGRITIAEFSTPNLPGFKTIYK
EYLTKLLPLIAKVVGTNPEAYEYLAESIRAWPEREELAAAINENGWHDAGWQNLSLGIVALHSAVKPS

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=267, Percent_Identity=31.4606741573034, Blast_Score=144, Evalue=6e-35,
Organism=Escherichia coli, GI48994983, Length=226, Percent_Identity=38.9380530973451, Blast_Score=164, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17557039, Length=233, Percent_Identity=32.1888412017167, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6323526, Length=245, Percent_Identity=30.6122448979592, Blast_Score=105, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24641702, Length=244, Percent_Identity=31.5573770491803, Blast_Score=136, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 25228; Mature: 25097

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS01183 UBIE_1 ; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKASLDKKPLDVAKMFDGVGKNYDITNTVLSFGRDKKWREKTRERLQLKAGERVLDLAA
CCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHC
GTAVSTVELTRSGAWVVACDFSQGMLAAGKDRNVPKVVGDGMKLPFADNSFDAVTISYGL
CCHHEEEEEECCCCEEEEEECCCCCEECCCCCCCCHHHCCCCCCCCCCCCCCEEEEECCH
RNVHDFRMALREMARVTKPGGRITIAEFSTPNLPGFKTIYKEYLTKLLPLIAKVVGTNPE
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
AYEYLAESIRAWPEREELAAAINENGWHDAGWQNLSLGIVALHSAVKPS
HHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKASLDKKPLDVAKMFDGVGKNYDITNTVLSFGRDKKWREKTRERLQLKAGERVLDLAA
CCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHC
GTAVSTVELTRSGAWVVACDFSQGMLAAGKDRNVPKVVGDGMKLPFADNSFDAVTISYGL
CCHHEEEEEECCCCEEEEEECCCCCEECCCCCCCCHHHCCCCCCCCCCCCCCEEEEECCH
RNVHDFRMALREMARVTKPGGRITIAEFSTPNLPGFKTIYKEYLTKLLPLIAKVVGTNPE
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
AYEYLAESIRAWPEREELAAAINENGWHDAGWQNLSLGIVALHSAVKPS
HHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA