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The map label for this gene is thiC

Identifier: 161579523

GI number: 161579523

Start: 956917

End: 958197

Strand: Reverse

Name: thiC

Synonym: MJ1026

Alternate gene names: 161579523

Gene position: 958197-956917 (Counterclockwise)

Preceding gene: 15669224

Following gene: 15669214

Centisome position: 57.55

GC content: 35.28

Gene sequence:

>1281_bases
ATGACTCAAATGGATGATGCAAAAAATGGGATTATCACTGAAGAGATGAAAATCGTTGCTGAAAAAGAAAAAATTGATAT
TGAAAAGCTTAGAAAACTTATAGCAAAAGGATATGTAGTTATTTTAAAGAATGTTAATAGGGATACAAATCCAGTAGGAA
TTGGGCAGAGTTTAAGAACTAAAGTAAATGCAAACATTGGGACGTCTCCAGATTGTGTTGATATAGAATTGGAGATAAAA
AAGGCAAAAATTGCTGAAAAATATGGGGCAGATGCAGTAATGGATTTAAGCACTGGAGGTAATTTGGAAGAGATAAGAAA
AGCGATAATGGATGCTGTTAAAATCCCTATTGGGACAGTTCCAATATATGAAGTTGGAAAATTGGCAAGAGAAAAGTATG
GAAGAGTCATTGATATGAATGAAGATTTGATGTTTAAGGTTATTGAAAAGCAAGCTAAAGAAGGAGTAGATTTTATGACT
TTACATTGTGGTATAACTAAACAGTCAGTTGAGAGATTAAAGAGAAGTGGAAGAATAATGGGAGTTGTAAGTAGAGGAGG
AGCATTTTTAACCGCCTATATCTTATATCACAACGAAGAAAACCCATTATACAAAAACTTTGATTATTTATTAGATATCC
TTAAAGAGCATGATGTAACTATAAGCTTAGGAGATGGAATGAGACCTGGTTGCTTAGCAGATAACACAGATAGGGCTCAA
ATTGAAGAGCTCATTACTTTAGGAGAGTTGGTTGAAAGATGTAGGGAGAAAGGAGTTCAATGTATGGTTGAAGGGCCAGG
ACATATTCCTATAAACTACATAGAAACAAACATCAGATTGCAAAAAAGTTTATGTAAAAATGCTCCATTCTACGTTTTGG
GGCCGATAGTTACAGATATAGCCCCTGGCTATGACCATATAACTGCTGCAATTGGTGGAGCTTTAGCAGGCTATTATGGA
GCTGATTTCCTCTGCTATGTAACTCCAAGTGAGCATTTAAGATTGCCTACAATAGAAGATGTTAAAGAAGGAGTTATAGC
TACTAAAATAGCTGCTCAAGCTGCTGATGTTGCTAAAGGGAATAAATTAGCATGGGAAAAAGAGACAGAGATGGCTTATG
CAAGGAAAAACCATGATTGGGAAAAGCAGTTTGAATTAGCAATAGATAAGGAGAAGGCAAGAAAGATGAGAGAAGAAATT
CCATCAAAAGAAGAAAAGGCATGTTCAATTTGTGGGGATTACTGCGCTTTGTTAATGGTTGAAGAGTTAGGAAAGAGATA
A

Upstream 100 bases:

>100_bases
TCATAATTAATAATGATAATTAGCTTAACCTTTTATATAGTTTTGTTTTAATAAAGTTATCGAGATGCATTATTATTTTA
TTACAAATCTGGTGATTGTT

Downstream 100 bases:

>100_bases
AATGTGGTGTCTATTATGAACAAAAATGAATTAATAACTGAAATTTTAAAAAATGAGGTAGTTAAGGCGTTAGGTTGCAC
AGAAGTTGGATTAATTGGTT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTQMDDAKNGIITEEMKIVAEKEKIDIEKLRKLIAKGYVVILKNVNRDTNPVGIGQSLRTKVNANIGTSPDCVDIELEIK
KAKIAEKYGADAVMDLSTGGNLEEIRKAIMDAVKIPIGTVPIYEVGKLAREKYGRVIDMNEDLMFKVIEKQAKEGVDFMT
LHCGITKQSVERLKRSGRIMGVVSRGGAFLTAYILYHNEENPLYKNFDYLLDILKEHDVTISLGDGMRPGCLADNTDRAQ
IEELITLGELVERCREKGVQCMVEGPGHIPINYIETNIRLQKSLCKNAPFYVLGPIVTDIAPGYDHITAAIGGALAGYYG
ADFLCYVTPSEHLRLPTIEDVKEGVIATKIAAQAADVAKGNKLAWEKETEMAYARKNHDWEKQFELAIDKEKARKMREEI
PSKEEKACSICGDYCALLMVEELGKR

Sequences:

>Translated_426_residues
MTQMDDAKNGIITEEMKIVAEKEKIDIEKLRKLIAKGYVVILKNVNRDTNPVGIGQSLRTKVNANIGTSPDCVDIELEIK
KAKIAEKYGADAVMDLSTGGNLEEIRKAIMDAVKIPIGTVPIYEVGKLAREKYGRVIDMNEDLMFKVIEKQAKEGVDFMT
LHCGITKQSVERLKRSGRIMGVVSRGGAFLTAYILYHNEENPLYKNFDYLLDILKEHDVTISLGDGMRPGCLADNTDRAQ
IEELITLGELVERCREKGVQCMVEGPGHIPINYIETNIRLQKSLCKNAPFYVLGPIVTDIAPGYDHITAAIGGALAGYYG
ADFLCYVTPSEHLRLPTIEDVKEGVIATKIAAQAADVAKGNKLAWEKETEMAYARKNHDWEKQFELAIDKEKARKMREEI
PSKEEKACSICGDYCALLMVEELGKR
>Mature_425_residues
TQMDDAKNGIITEEMKIVAEKEKIDIEKLRKLIAKGYVVILKNVNRDTNPVGIGQSLRTKVNANIGTSPDCVDIELEIKK
AKIAEKYGADAVMDLSTGGNLEEIRKAIMDAVKIPIGTVPIYEVGKLAREKYGRVIDMNEDLMFKVIEKQAKEGVDFMTL
HCGITKQSVERLKRSGRIMGVVSRGGAFLTAYILYHNEENPLYKNFDYLLDILKEHDVTISLGDGMRPGCLADNTDRAQI
EELITLGELVERCREKGVQCMVEGPGHIPINYIETNIRLQKSLCKNAPFYVLGPIVTDIAPGYDHITAAIGGALAGYYGA
DFLCYVTPSEHLRLPTIEDVKEGVIATKIAAQAADVAKGNKLAWEKETEMAYARKNHDWEKQFELAIDKEKARKMREEIP
SKEEKACSICGDYCALLMVEELGKR

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=460, Percent_Identity=41.304347826087, Blast_Score=344, Evalue=6e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_METJA (Q58432)

Other databases:

- EMBL:   L77117
- PIR:   A64428
- RefSeq:   NP_248020.2
- GeneID:   1451923
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_1026
- NMPDR:   fig|243232.1.peg.1057
- TIGR:   MJ1026
- HOGENOM:   HBG285234
- OMA:   VDFMTIH
- ProtClustDB:   PRK13352
- BioCyc:   MJAN243232:MJ_1026-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 47478; Mature: 47346

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: BINDING 65-65 BINDING 94-94 BINDING 123-123 BINDING 162-162 BINDING 264-264 BINDING 291-291

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQMDDAKNGIITEEMKIVAEKEKIDIEKLRKLIAKGYVVILKNVNRDTNPVGIGQSLRT
CCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHH
KVNANIGTSPDCVDIELEIKKAKIAEKYGADAVMDLSTGGNLEEIRKAIMDAVKIPIGTV
HCCCCCCCCCCEEEEEEEEHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCC
PIYEVGKLAREKYGRVIDMNEDLMFKVIEKQAKEGVDFMTLHCGITKQSVERLKRSGRIM
CHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCEE
GVVSRGGAFLTAYILYHNEENPLYKNFDYLLDILKEHDVTISLGDGMRPGCLADNTDRAQ
EEEECCCCEEEEEEEEECCCCCCHHCHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHH
IEELITLGELVERCREKGVQCMVEGPGHIPINYIETNIRLQKSLCKNAPFYVLGPIVTDI
HHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCHHHHHHHHCCCCCEEECCHHHHC
APGYDHITAAIGGALAGYYGADFLCYVTPSEHLRLPTIEDVKEGVIATKIAAQAADVAKG
CCCHHHHHHHHHHHHHHHHCCCEEEEECCHHCEECCCHHHHHCCHHHHHHHHHHHHHCCC
NKLAWEKETEMAYARKNHDWEKQFELAIDKEKARKMREEIPSKEEKACSICGDYCALLMV
CCCCCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EELGKR
HHHCCC
>Mature Secondary Structure 
TQMDDAKNGIITEEMKIVAEKEKIDIEKLRKLIAKGYVVILKNVNRDTNPVGIGQSLRT
CCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHH
KVNANIGTSPDCVDIELEIKKAKIAEKYGADAVMDLSTGGNLEEIRKAIMDAVKIPIGTV
HCCCCCCCCCCEEEEEEEEHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCC
PIYEVGKLAREKYGRVIDMNEDLMFKVIEKQAKEGVDFMTLHCGITKQSVERLKRSGRIM
CHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCEE
GVVSRGGAFLTAYILYHNEENPLYKNFDYLLDILKEHDVTISLGDGMRPGCLADNTDRAQ
EEEECCCCEEEEEEEEECCCCCCHHCHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHH
IEELITLGELVERCREKGVQCMVEGPGHIPINYIETNIRLQKSLCKNAPFYVLGPIVTDI
HHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCHHHHHHHHCCCCCEEECCHHHHC
APGYDHITAAIGGALAGYYGADFLCYVTPSEHLRLPTIEDVKEGVIATKIAAQAADVAKG
CCCHHHHHHHHHHHHHHHHCCCEEEEECCHHCEECCCHHHHHCCHHHHHHHHHHHHHCCC
NKLAWEKETEMAYARKNHDWEKQFELAIDKEKARKMREEIPSKEEKACSICGDYCALLMV
CCCCCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EELGKR
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087