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The map label for this gene is gatA

Identifier: 161526274

GI number: 161526274

Start: 3390806

End: 3392305

Strand: Reverse

Name: gatA

Synonym: Bmul_3105

Alternate gene names: 161526274

Gene position: 3392305-3390806 (Counterclockwise)

Preceding gene: 161526275

Following gene: 161526273

Centisome position: 98.37

GC content: 69.07

Gene sequence:

>1500_bases
ATGCACGCAAAAAGCCTGACCGAACTGCGCGCCGCGCTCGCCGCCAAGGAATGCTCGGCCGTCGAGCTCGCGCAGCACTA
TCTGAAACGGATCGACGCCGCGCGCGACCTGAACGCGTTCGTCCACGTCGAGCCCGAACTCACCCTCGCGCAGGCGAAGG
CCGCCGATGCCGAACTCGCGCGCGGCGCGGGCGGCCCGCTGACGGGCCTGCCGATCGCGCACAAGGACGTGTTCGTCACG
CGCGGCTGGCGCTCGACGGCCGGCTCGAAGATGCTCGCCAATTACGAGAGCCCGTTCGACGCGACCGTCGTCGCCCGCCT
GCAGGCGGCCGGCATGGTCACGCTCGGCAAGACCAACATGGACGAGTTCGCGATGGGCTCGTCGAACGAGAATTCCGCGT
TCGGCGCGGTGAAGAACCCGTGGGACACGAACGCCGTGCCGGGCGGCAGCTCGGGCGGCAGCTCGGCCGCGGTGGCCGCG
CGTCTCGCGCCGGCCGCGACCGGCACCGACACCGGCGGCTCGATCCGCCAGCCGGCGTCGTTCGCGGGCGTGACCGGCAT
CAAGCCGACCTACGGCCGCGTGTCGCGCTACGGCATGATCGCGTTCGCGTCGTCGCTCGACCAGGGCGGCCCGATGGCGC
AGAGCGCGTCCGACTGCGCGCTGCTGCTCAACGCGATGGCCGGCTTCGACGAGCGCGACTCGACGAGCCTCGAGCGCGAC
GACGAAGACTTCGCGCGCCACCTCGGCCAGCCGTGGGCACCGGGCAACGACGCGGGCAAGCCGCTCGCCGGCCTGCGCAT
CGGCCTGCCGAACGAGTATTTCGGCGACGGTCTCGCCGACGACGTGCGCGCGGCGATCGACGCGGCACTCAAGCAATATG
AAGCGCTCGGCGCGACGCTCGTGCCGGTGTCGCTGCCGAAGACGGAGCTGTCGATTCCCGTGTACTACGTGATCGCGCCG
GCCGAGGCCTCGTCGAACCTGTCGCGGTTCGACGGCGTGCGCTTCGGCCATCGCGCCGCGCAGTACGGCGATCTGCTCGA
CATGTACAAGAAGTCGCGCGCCGAAGGCTTCGGGCCCGAAGTGAAGCGCCGCATTCTCGTCGGCACGTATGTGCTGTCGC
ACGGCTACTACGACGCGTATTACCTGCAGGCGCAGAAGATCCGCCGCATCATCGCGAACGACTTCCAGGAAGCATTCAAG
TCCTGCGACGTGATCATGGGCCCGGCGTCGCCGACGGTCGCATGGGATCTCGGCGCGAAGGGCGACGATCCGGTGCAGAT
GTATCTGGCCGATATCTACACGCTGTCGGTGAGCCTCGCCGGCCTGCCCGGCATGAGCGTGCCGTGCGGCTTCGGCGCCG
GCGCGAACGCGAAGCGTCCGGTCGGCCTGCAGATCATCGGCAACTATTTCAACGAAGCCCGGATGCTGCAGGTCGCCGAC
GCGTTCCAGCGCGCGACCGACTGGCACAAGCAAGTTCCGCAAGTTCCGGCAGGGGTGTAA

Upstream 100 bases:

>100_bases
CTCTATCTCGTGCCGAAGGTGATCGAGTAAACGCTCGACGACAAGCGCGTCGGCCCGCACGGCGCCGCGCGCGACCCAGA
ATTCCCAGGAAGACTCCCTC

Downstream 100 bases:

>100_bases
GCACATGTCTACTCAGTGGGAAGTCGTCATCGGTCTCGAGACGCACGCGCAACTGTCGACCGTCTCGAAGATTTTCTCCG
GCGCCTCGACGCAGTTCGGC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MHAKSLTELRAALAAKECSAVELAQHYLKRIDAARDLNAFVHVEPELTLAQAKAADAELARGAGGPLTGLPIAHKDVFVT
RGWRSTAGSKMLANYESPFDATVVARLQAAGMVTLGKTNMDEFAMGSSNENSAFGAVKNPWDTNAVPGGSSGGSSAAVAA
RLAPAATGTDTGGSIRQPASFAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMAQSASDCALLLNAMAGFDERDSTSLERD
DEDFARHLGQPWAPGNDAGKPLAGLRIGLPNEYFGDGLADDVRAAIDAALKQYEALGATLVPVSLPKTELSIPVYYVIAP
AEASSNLSRFDGVRFGHRAAQYGDLLDMYKKSRAEGFGPEVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIANDFQEAFK
SCDVIMGPASPTVAWDLGAKGDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAKRPVGLQIIGNYFNEARMLQVAD
AFQRATDWHKQVPQVPAGV

Sequences:

>Translated_499_residues
MHAKSLTELRAALAAKECSAVELAQHYLKRIDAARDLNAFVHVEPELTLAQAKAADAELARGAGGPLTGLPIAHKDVFVT
RGWRSTAGSKMLANYESPFDATVVARLQAAGMVTLGKTNMDEFAMGSSNENSAFGAVKNPWDTNAVPGGSSGGSSAAVAA
RLAPAATGTDTGGSIRQPASFAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMAQSASDCALLLNAMAGFDERDSTSLERD
DEDFARHLGQPWAPGNDAGKPLAGLRIGLPNEYFGDGLADDVRAAIDAALKQYEALGATLVPVSLPKTELSIPVYYVIAP
AEASSNLSRFDGVRFGHRAAQYGDLLDMYKKSRAEGFGPEVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIANDFQEAFK
SCDVIMGPASPTVAWDLGAKGDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAKRPVGLQIIGNYFNEARMLQVAD
AFQRATDWHKQVPQVPAGV
>Mature_499_residues
MHAKSLTELRAALAAKECSAVELAQHYLKRIDAARDLNAFVHVEPELTLAQAKAADAELARGAGGPLTGLPIAHKDVFVT
RGWRSTAGSKMLANYESPFDATVVARLQAAGMVTLGKTNMDEFAMGSSNENSAFGAVKNPWDTNAVPGGSSGGSSAAVAA
RLAPAATGTDTGGSIRQPASFAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMAQSASDCALLLNAMAGFDERDSTSLERD
DEDFARHLGQPWAPGNDAGKPLAGLRIGLPNEYFGDGLADDVRAAIDAALKQYEALGATLVPVSLPKTELSIPVYYVIAP
AEASSNLSRFDGVRFGHRAAQYGDLLDMYKKSRAEGFGPEVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIANDFQEAFK
SCDVIMGPASPTVAWDLGAKGDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAKRPVGLQIIGNYFNEARMLQVAD
AFQRATDWHKQVPQVPAGV

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=512, Percent_Identity=36.9140625, Blast_Score=315, Evalue=6e-86,
Organism=Homo sapiens, GI195972892, Length=495, Percent_Identity=26.0606060606061, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI166795287, Length=135, Percent_Identity=38.5185185185185, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=475, Percent_Identity=36.8421052631579, Blast_Score=267, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17537465, Length=238, Percent_Identity=32.7731092436975, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17556264, Length=192, Percent_Identity=31.7708333333333, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=323, Percent_Identity=26.625386996904, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71990152, Length=229, Percent_Identity=30.1310043668122, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=192, Percent_Identity=32.8125, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=192, Percent_Identity=32.8125, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=345, Percent_Identity=25.7971014492754, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=444, Percent_Identity=33.5585585585586, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6319685, Length=424, Percent_Identity=28.5377358490566, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320448, Length=237, Percent_Identity=28.2700421940928, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648113, Length=490, Percent_Identity=39.7959183673469, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24652985, Length=505, Percent_Identity=26.3366336633663, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI19922090, Length=505, Percent_Identity=26.3366336633663, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24652981, Length=505, Percent_Identity=26.3366336633663, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24652983, Length=505, Percent_Identity=26.3366336633663, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24644968, Length=524, Percent_Identity=25.1908396946565, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161078093, Length=532, Percent_Identity=25.3759398496241, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356731, Length=509, Percent_Identity=23.3791748526523, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24648435, Length=235, Percent_Identity=29.7872340425532, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648437, Length=235, Percent_Identity=29.7872340425532, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45550774, Length=235, Percent_Identity=29.7872340425532, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648441, Length=167, Percent_Identity=33.5329341317365, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648439, Length=167, Percent_Identity=33.5329341317365, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_BURM1 (A9AC51)

Other databases:

- EMBL:   CP000868
- EMBL:   AP009385
- RefSeq:   YP_001581286.1
- RefSeq:   YP_001944640.1
- ProteinModelPortal:   A9AC51
- SMR:   A9AC51
- GeneID:   5765524
- GeneID:   6359378
- GenomeReviews:   AP009385_GR
- GenomeReviews:   CP000868_GR
- KEGG:   bmj:BMULJ_00126
- KEGG:   bmu:Bmul_3105
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52792; Mature: 52792

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 75-75 ACT_SITE 150-150 ACT_SITE 174-174

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAKSLTELRAALAAKECSAVELAQHYLKRIDAARDLNAFVHVEPELTLAQAKAADAELA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEHHHHHHHHHHHH
RGAGGPLTGLPIAHKDVFVTRGWRSTAGSKMLANYESPFDATVVARLQAAGMVTLGKTNM
CCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHCCCCCCCHHHHHHHHHCCEEEECCCCH
DEFAMGSSNENSAFGAVKNPWDTNAVPGGSSGGSSAAVAARLAPAATGTDTGGSIRQPAS
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCH
FAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMAQSASDCALLLNAMAGFDERDSTSLERD
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
DEDFARHLGQPWAPGNDAGKPLAGLRIGLPNEYFGDGLADDVRAAIDAALKQYEALGATL
HHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCEE
VPVSLPKTELSIPVYYVIAPAEASSNLSRFDGVRFGHRAAQYGDLLDMYKKSRAEGFGPE
EEEECCCCCCCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
VKRRILVGTYVLSHGYYDAYYLQAQKIRRIIANDFQEAFKSCDVIMGPASPTVAWDLGAK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEECCCC
GDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAKRPVGLQIIGNYFNEARMLQVAD
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AFQRATDWHKQVPQVPAGV
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MHAKSLTELRAALAAKECSAVELAQHYLKRIDAARDLNAFVHVEPELTLAQAKAADAELA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEHHHHHHHHHHHH
RGAGGPLTGLPIAHKDVFVTRGWRSTAGSKMLANYESPFDATVVARLQAAGMVTLGKTNM
CCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHCCCCCCCHHHHHHHHHCCEEEECCCCH
DEFAMGSSNENSAFGAVKNPWDTNAVPGGSSGGSSAAVAARLAPAATGTDTGGSIRQPAS
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCH
FAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMAQSASDCALLLNAMAGFDERDSTSLERD
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
DEDFARHLGQPWAPGNDAGKPLAGLRIGLPNEYFGDGLADDVRAAIDAALKQYEALGATL
HHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCEE
VPVSLPKTELSIPVYYVIAPAEASSNLSRFDGVRFGHRAAQYGDLLDMYKKSRAEGFGPE
EEEECCCCCCCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
VKRRILVGTYVLSHGYYDAYYLQAQKIRRIIANDFQEAFKSCDVIMGPASPTVAWDLGAK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEECCCC
GDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAKRPVGLQIIGNYFNEARMLQVAD
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AFQRATDWHKQVPQVPAGV
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA