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The map label for this gene is gatB [H]

Identifier: 161526273

GI number: 161526273

Start: 3389326

End: 3390801

Strand: Reverse

Name: gatB [H]

Synonym: Bmul_3104

Alternate gene names: 161526273

Gene position: 3390801-3389326 (Counterclockwise)

Preceding gene: 161526274

Following gene: 161526272

Centisome position: 98.33

GC content: 65.58

Gene sequence:

>1476_bases
ATGTCTACTCAGTGGGAAGTCGTCATCGGTCTCGAGACGCACGCGCAACTGTCGACCGTCTCGAAGATTTTCTCCGGCGC
CTCGACGCAGTTCGGCGCCGAGCCGAACACGCAGGCCTGCCCGGTCGACCTCGCGCTGCCCGGCGTGCTGCCGGTGCTGA
ACCGCGGCGCGGTCGAGCGTGCGATCCGCTTCGGCCTCGCGATCGGCGCGACCATCGCGCCGCGCAGCATCTTCGCGCGC
AAGAATTATTTCTATCCCGATCTGCCGAAGGGCTATCAGATCAGCCAGTACGAGATTCCGGTCGTGCAGGGCGGCGCGAT
CACGATCCAGGTGCCGGCCAACGAGAAGGCCGGCAAGCCGGCGTACGAGAAGACCGTCAACCTGACCCGCGCGCACCTCG
AGGAAGATGCGGGCAAGTCGCTGCATGAAGACTTCGCCGGCATGACGGGCATCGACCTGAACCGCGCGGGCACGCCGCTG
CTCGAGATCGTCACCGAGCCGGAAATGCGCAGCGCGGCCGAAGCCGTCGCCTACGCGAAGGCGCTGCATGCGCTCGTCGT
GTGGCTCGGCATCTGCGACGGCAACATGCAGGAAGGCTCGTTCCGCTGCGACGCGAACGTGTCGGTGCGTCCGGTCGGTC
AGGAGAAGTTCGGCACGCGCGCCGAGATCAAGAACCTGAACTCGTTCCGCTTCCTCGAGGAAGCGATCAACTACGAAGTG
CGTCGCCAGATCGAGCTGATCGAGGACGGCGGCGAGGTCGTGCAGGAAACGCGTCTGTACGATCCGGACAAGCGCGAAAC
GCGCTCGATGCGCAGCAAGGAAGACGCGCACGATTACCGCTACTTCCCCGATCCCGACCTGATGCCGCTCGTGATCGGCC
GCGACTGGGTCGAGCGCGTGCAGGCCGGGATGCCCGAGCTGCCGGCCGCGATGCAGCAGCGCTTCGTCGAGCAATATGGC
GTGTCCGCGTACGACGCCGGCGTGCTGACGTCGACGAAGGCGATGGCCGCGTACTTCGAGGCCGTCGTCGCGAAGGCGGG
CGCGGCGAACGCGAAGATCGCCGCGAACTGGCTGATGGGCGACGTGTCGTCGCAGCTGAACCGCGACGGCATCGAGATCG
ACGCGATTCCGGTATCGGCCGCGCAGCTCGCGCTGCTGCTGCAGCGTATCGCCGACGGCACGATCTCGAACAAGATCGCG
AAGGAAGTGTTCGCGACGATGTGGGAAGAGAAGGCGAGCGACGACGGCGCGGCCGATCGCATCATCGAGGCGAAGGGCCT
GAAGCAGATCTCCGACACCGGCGCGCTGGAAGCGATCATCGACGAGGTGCTCGCGGCGAACGCGAAGTCGGTCGAGGAAT
TCCGCGCGGGCAAGGAAAAGGCGTTCAACGCACTGATCGGCCAGGCGATGAAGGCGACCAAGGGCAAGGCCAATCCGCAG
CAGGTCAACGAACTGCTGAAGAAGAAGCTCGGCTGA

Upstream 100 bases:

>100_bases
TATTTCAACGAAGCCCGGATGCTGCAGGTCGCCGACGCGTTCCAGCGCGCGACCGACTGGCACAAGCAAGTTCCGCAAGT
TCCGGCAGGGGTGTAAGCAC

Downstream 100 bases:

>100_bases
ACATGACCGCGCGCCTGAACCGCGCCTGACGACGGGCCGCTGCCGAACCTGTTCGGCGCGGCCCGTCGCGTTTCGATGCG
AGCGCCCGCGCGCCGCGTCG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MSTQWEVVIGLETHAQLSTVSKIFSGASTQFGAEPNTQACPVDLALPGVLPVLNRGAVERAIRFGLAIGATIAPRSIFAR
KNYFYPDLPKGYQISQYEIPVVQGGAITIQVPANEKAGKPAYEKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPL
LEIVTEPEMRSAAEAVAYAKALHALVVWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRAEIKNLNSFRFLEEAINYEV
RRQIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGRDWVERVQAGMPELPAAMQQRFVEQYG
VSAYDAGVLTSTKAMAAYFEAVVAKAGAANAKIAANWLMGDVSSQLNRDGIEIDAIPVSAAQLALLLQRIADGTISNKIA
KEVFATMWEEKASDDGAADRIIEAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKEKAFNALIGQAMKATKGKANPQ
QVNELLKKKLG

Sequences:

>Translated_491_residues
MSTQWEVVIGLETHAQLSTVSKIFSGASTQFGAEPNTQACPVDLALPGVLPVLNRGAVERAIRFGLAIGATIAPRSIFAR
KNYFYPDLPKGYQISQYEIPVVQGGAITIQVPANEKAGKPAYEKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPL
LEIVTEPEMRSAAEAVAYAKALHALVVWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRAEIKNLNSFRFLEEAINYEV
RRQIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGRDWVERVQAGMPELPAAMQQRFVEQYG
VSAYDAGVLTSTKAMAAYFEAVVAKAGAANAKIAANWLMGDVSSQLNRDGIEIDAIPVSAAQLALLLQRIADGTISNKIA
KEVFATMWEEKASDDGAADRIIEAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKEKAFNALIGQAMKATKGKANPQ
QVNELLKKKLG
>Mature_490_residues
STQWEVVIGLETHAQLSTVSKIFSGASTQFGAEPNTQACPVDLALPGVLPVLNRGAVERAIRFGLAIGATIAPRSIFARK
NYFYPDLPKGYQISQYEIPVVQGGAITIQVPANEKAGKPAYEKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLL
EIVTEPEMRSAAEAVAYAKALHALVVWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRAEIKNLNSFRFLEEAINYEVR
RQIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGRDWVERVQAGMPELPAAMQQRFVEQYGV
SAYDAGVLTSTKAMAAYFEAVVAKAGAANAKIAANWLMGDVSSQLNRDGIEIDAIPVSAAQLALLLQRIADGTISNKIAK
EVFATMWEEKASDDGAADRIIEAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKEKAFNALIGQAMKATKGKANPQQ
VNELLKKKLG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=500, Percent_Identity=39.2, Blast_Score=357, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI115534094, Length=466, Percent_Identity=32.4034334763949, Blast_Score=230, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6319391, Length=523, Percent_Identity=31.3575525812619, Blast_Score=225, Evalue=1e-59,
Organism=Drosophila melanogaster, GI45550808, Length=497, Percent_Identity=35.2112676056338, Blast_Score=325, Evalue=4e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53446; Mature: 53315

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQWEVVIGLETHAQLSTVSKIFSGASTQFGAEPNTQACPVDLALPGVLPVLNRGAVER
CCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHCCHHHHH
AIRFGLAIGATIAPRSIFARKNYFYPDLPKGYQISQYEIPVVQGGAITIQVPANEKAGKP
HHHHHHHHCCHHCCHHHHHHCCCCCCCCCCCCCCCEEECEEEECCEEEEEECCCCCCCCC
AYEKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPEMRSAAEAVAYAK
HHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHH
ALHALVVWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRAEIKNLNSFRFLEEAINYEV
HHHHHHHHHHHCCCCCCCCCEEECCCCEEEECCHHHCCCHHHHHCCHHHHHHHHHHCHHH
RRQIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGRDWVERV
HHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHHCHHHHHHH
QAGMPELPAAMQQRFVEQYGVSAYDAGVLTSTKAMAAYFEAVVAKAGAANAKIAANWLMG
HCCCCCCHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
DVSSQLNRDGIEIDAIPVSAAQLALLLQRIADGTISNKIAKEVFATMWEEKASDDGAADR
HHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHH
IIEAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKEKAFNALIGQAMKATKGKANPQ
HHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHH
QVNELLKKKLG
HHHHHHHHHCC
>Mature Secondary Structure 
STQWEVVIGLETHAQLSTVSKIFSGASTQFGAEPNTQACPVDLALPGVLPVLNRGAVER
CCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHCCHHHHH
AIRFGLAIGATIAPRSIFARKNYFYPDLPKGYQISQYEIPVVQGGAITIQVPANEKAGKP
HHHHHHHHCCHHCCHHHHHHCCCCCCCCCCCCCCCEEECEEEECCEEEEEECCCCCCCCC
AYEKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPEMRSAAEAVAYAK
HHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHH
ALHALVVWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRAEIKNLNSFRFLEEAINYEV
HHHHHHHHHHHCCCCCCCCCEEECCCCEEEECCHHHCCCHHHHHCCHHHHHHHHHHCHHH
RRQIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGRDWVERV
HHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHHCHHHHHHH
QAGMPELPAAMQQRFVEQYGVSAYDAGVLTSTKAMAAYFEAVVAKAGAANAKIAANWLMG
HCCCCCCHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
DVSSQLNRDGIEIDAIPVSAAQLALLLQRIADGTISNKIAKEVFATMWEEKASDDGAADR
HHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHH
IIEAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKEKAFNALIGQAMKATKGKANPQ
HHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHH
QVNELLKKKLG
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA