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The map label for this gene is gacA [H]

Identifier: 161526221

GI number: 161526221

Start: 3335436

End: 3336092

Strand: Direct

Name: gacA [H]

Synonym: Bmul_3052

Alternate gene names: 161526221

Gene position: 3335436-3336092 (Clockwise)

Preceding gene: 161526220

Following gene: 161526222

Centisome position: 96.72

GC content: 65.91

Gene sequence:

>657_bases
ATGGGCCTGAACATCCTGCTCGTGGACGACCACGCGATCGTCCGGCAAGGGATCTGCCAGCTGCTCGTCGACCGCGGCAT
CGCACGCGAGGTCACCGAAGCGGAAAACGGCGCCGACGCGCTCGCCGCCGTCGACCGGCAGACGTTCGACGTGATCCTGC
TCGACATCTCGCTGCCCGACACGAACGGCATCGAGGTGCTCAAGCGCCTGAAGCGCAAGCTGCCGCGCACGCCGGTGCTG
ATGTTCTCGATGTACCGCGAGGATCAGTACGCGGTGCGCGCGCTGAAGGCCGGCGCCGCCGGCTATCTGTCGAAGACGGT
CAACGCCGCACAGATGATCGGCGCGATCCAGCAGGTCGCCGCGGGCCGCAAGTACGTGAGCCCCGCGATGGCCGAAGCGC
TCGCCGAATACGTGTCGTTCGAGAACGAGCCGCTGCCGCACGAAAAGCTGTCCGACCGCGAATATCAGACGCTCTGCATG
CTCGCCTCGGGCAAGCGGCTCACCGACATCGCGAACACGCTGTCGCTGTCGGTGAAGACGGTCAGCGTGTACCGCTCGCG
GCTGCTCGAGAAGATGCGGCTGTCGAACAACGCGGAACTCACGTTCTACGTGATGAGCAACCGGCTCGTCGACATGTCGC
CCGCGTCGGGCACCTGA

Upstream 100 bases:

>100_bases
GCGGCCGGGCGCGGCACGCGCGTGACCGCGTACTTCGCGTGGGACGCGCTGCTCGGCGCGCCCGCCGCGGCCCGTTTCGC
ATCGCTGTCGTGAGGTCGCG

Downstream 100 bases:

>100_bases
CGCGCCGCGCACACCCTCTCGCTGCCCCACCCGCACGGTTGCGGCGCGGCCGCTCGGCCGCCGCTCGGCCGTTGCGCACG
CAAGCGCTTGTTTTATCGGA

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MGLNILLVDDHAIVRQGICQLLVDRGIAREVTEAENGADALAAVDRQTFDVILLDISLPDTNGIEVLKRLKRKLPRTPVL
MFSMYREDQYAVRALKAGAAGYLSKTVNAAQMIGAIQQVAAGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCM
LASGKRLTDIANTLSLSVKTVSVYRSRLLEKMRLSNNAELTFYVMSNRLVDMSPASGT

Sequences:

>Translated_218_residues
MGLNILLVDDHAIVRQGICQLLVDRGIAREVTEAENGADALAAVDRQTFDVILLDISLPDTNGIEVLKRLKRKLPRTPVL
MFSMYREDQYAVRALKAGAAGYLSKTVNAAQMIGAIQQVAAGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCM
LASGKRLTDIANTLSLSVKTVSVYRSRLLEKMRLSNNAELTFYVMSNRLVDMSPASGT
>Mature_217_residues
GLNILLVDDHAIVRQGICQLLVDRGIAREVTEAENGADALAAVDRQTFDVILLDISLPDTNGIEVLKRLKRKLPRTPVLM
FSMYREDQYAVRALKAGAAGYLSKTVNAAQMIGAIQQVAAGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCML
ASGKRLTDIANTLSLSVKTVSVYRSRLLEKMRLSNNAELTFYVMSNRLVDMSPASGT

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=211, Percent_Identity=34.1232227488152, Blast_Score=137, Evalue=4e-34,
Organism=Escherichia coli, GI1790102, Length=198, Percent_Identity=37.3737373737374, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1788521, Length=207, Percent_Identity=31.4009661835749, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788712, Length=209, Percent_Identity=28.2296650717703, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1786747, Length=213, Percent_Identity=30.5164319248826, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI1787473, Length=210, Percent_Identity=32.3809523809524, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1786599, Length=156, Percent_Identity=30.7692307692308, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788546, Length=216, Percent_Identity=25.9259259259259, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1790437, Length=106, Percent_Identity=39.622641509434, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1790299, Length=114, Percent_Identity=30.7017543859649, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1788550, Length=104, Percent_Identity=36.5384615384615, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1788191, Length=112, Percent_Identity=33.0357142857143, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24053; Mature: 23922

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLNILLVDDHAIVRQGICQLLVDRGIAREVTEAENGADALAAVDRQTFDVILLDISLPD
CCCEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCEEEEEEEEECCC
TNGIEVLKRLKRKLPRTPVLMFSMYREDQYAVRALKAGAAGYLSKTVNAAQMIGAIQQVA
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
AGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCMLASGKRLTDIANTLSLSVKT
CCCHHCCHHHHHHHHHHHCCCCCCCCCHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
VSVYRSRLLEKMRLSNNAELTFYVMSNRLVDMSPASGT
HHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCCC
>Mature Secondary Structure 
GLNILLVDDHAIVRQGICQLLVDRGIAREVTEAENGADALAAVDRQTFDVILLDISLPD
CCEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCEEEEEEEEECCC
TNGIEVLKRLKRKLPRTPVLMFSMYREDQYAVRALKAGAAGYLSKTVNAAQMIGAIQQVA
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
AGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCMLASGKRLTDIANTLSLSVKT
CCCHHCCHHHHHHHHHHHCCCCCCCCCHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
VSVYRSRLLEKMRLSNNAELTFYVMSNRLVDMSPASGT
HHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]