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The map label for this gene is potI [H]

Identifier: 161526162

GI number: 161526162

Start: 3273774

End: 3274613

Strand: Direct

Name: potI [H]

Synonym: Bmul_2993

Alternate gene names: 161526162

Gene position: 3273774-3274613 (Clockwise)

Preceding gene: 161526161

Following gene: 161526163

Centisome position: 94.93

GC content: 64.17

Gene sequence:

>840_bases
ATGATCAAGCCGAGCAAACCGCTGTCGACGGGCGTTCTCGCCTTCGGCTTCCTGTTTCTCTACATCCCGATCATCAGCCT
CGTCGTGTACTCGTTCAACGAGTCGAAGCTGGTCACCGTGTGGTCCGGCTTCTCGCTGAAGTGGTATGCGGCGCTCTGGC
AGGACGACGAGCTGCTGACGGCCGCCTGGCTGTCGCTGAAGATCGGCCTGCTGACCGCGACCGCGTCGGTCGTGATCGGC
ACCTGGGCCGGCTTCGTGCTCGCGCGCTTCGGGCGCTTCCGCGGCTTCACGCTCTACACGGGGATGATCAACGCGCCGCT
CGTGATTCCCGAAGTGATTCAAGGGATCTCGCTGCTGCTGTTGTTCGTCGCGCTCGAGCAGATGTTCGGCTGGCCGAAGG
GGCGCGGGATGGTGACGATCTGGATCGGCCACGTGATGCTCTGCGTGTCGTATGTCGCGATCATCGTGCAGTCCCGCGTG
AAGGAGATGAACAAGTCGCTCGAGGAGGCGGCGCTCGATCTCGGCGCGACGCCGTTGAAGGTGTTCTTCGTCGTCACGCT
GCCGCTGATCTCGCAGGCGCTGCTGTCGGGCTGGCTGCTGTCGTTCACGCTGTCGATCGACGATCTGGTGCTGTCGGCGT
TCCTGTCGGGCCCCGGCTCGACGACGCTGCCGCTCGTCGTGTTCTCGCGCGTGCGGCTCGGGCTCAATCCCGAGATGAAT
GCGCTCGCGACGCTGTTCATCACCGCGGTGACGATCGGCGTGATCGTCGTGAACCAGATGATGATCGCGCGCGAACGCCG
ACGCGTGGCCGATTTGAAGCACGCGTTCGCGGCCGCGTAA

Upstream 100 bases:

>100_bases
GGCCGATGGCGTCGGCGGTCACCGTCGCGATGGTGCTGCTGCTGCTCGTGCCGATGGCGTTGTTCCAGTACTACCAGGTC
AAGGAACTGGAGGAAGCGAA

Downstream 100 bases:

>100_bases
GCCCGCCCTTCCATCCGTACCGAAAACAACCCGGCGCGTCGCGTTGCGCGGCGCGCCGTTCGACCACGCACTGACGACGC
GCACGACAAGGAGAATCCGC

Product: ornithine carbamoyltransferase

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MIKPSKPLSTGVLAFGFLFLYIPIISLVVYSFNESKLVTVWSGFSLKWYAALWQDDELLTAAWLSLKIGLLTATASVVIG
TWAGFVLARFGRFRGFTLYTGMINAPLVIPEVIQGISLLLLFVALEQMFGWPKGRGMVTIWIGHVMLCVSYVAIIVQSRV
KEMNKSLEEAALDLGATPLKVFFVVTLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
ALATLFITAVTIGVIVVNQMMIARERRRVADLKHAFAAA

Sequences:

>Translated_279_residues
MIKPSKPLSTGVLAFGFLFLYIPIISLVVYSFNESKLVTVWSGFSLKWYAALWQDDELLTAAWLSLKIGLLTATASVVIG
TWAGFVLARFGRFRGFTLYTGMINAPLVIPEVIQGISLLLLFVALEQMFGWPKGRGMVTIWIGHVMLCVSYVAIIVQSRV
KEMNKSLEEAALDLGATPLKVFFVVTLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
ALATLFITAVTIGVIVVNQMMIARERRRVADLKHAFAAA
>Mature_279_residues
MIKPSKPLSTGVLAFGFLFLYIPIISLVVYSFNESKLVTVWSGFSLKWYAALWQDDELLTAAWLSLKIGLLTATASVVIG
TWAGFVLARFGRFRGFTLYTGMINAPLVIPEVIQGISLLLLFVALEQMFGWPKGRGMVTIWIGHVMLCVSYVAIIVQSRV
KEMNKSLEEAALDLGATPLKVFFVVTLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
ALATLFITAVTIGVIVVNQMMIARERRRVADLKHAFAAA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=270, Percent_Identity=56.2962962962963, Blast_Score=288, Evalue=3e-79,
Organism=Escherichia coli, GI1787368, Length=248, Percent_Identity=38.3064516129032, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI1787714, Length=245, Percent_Identity=32.2448979591837, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788764, Length=219, Percent_Identity=30.5936073059361, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30559; Mature: 30559

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKPSKPLSTGVLAFGFLFLYIPIISLVVYSFNESKLVTVWSGFSLKWYAALWQDDELLT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEHHHHHCCCHHHH
AAWLSLKIGLLTATASVVIGTWAGFVLARFGRFRGFTLYTGMINAPLVIPEVIQGISLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHH
LFVALEQMFGWPKGRGMVTIWIGHVMLCVSYVAIIVQSRVKEMNKSLEEAALDLGATPLK
HHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VFFVVTLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHH
ALATLFITAVTIGVIVVNQMMIARERRRVADLKHAFAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKPSKPLSTGVLAFGFLFLYIPIISLVVYSFNESKLVTVWSGFSLKWYAALWQDDELLT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEHHHHHCCCHHHH
AAWLSLKIGLLTATASVVIGTWAGFVLARFGRFRGFTLYTGMINAPLVIPEVIQGISLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHH
LFVALEQMFGWPKGRGMVTIWIGHVMLCVSYVAIIVQSRVKEMNKSLEEAALDLGATPLK
HHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VFFVVTLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHH
ALATLFITAVTIGVIVVNQMMIARERRRVADLKHAFAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]