Click here to switch to the map view.

The map label for this gene is potH [H]

Identifier: 161526161

GI number: 161526161

Start: 3272791

End: 3273777

Strand: Direct

Name: potH [H]

Synonym: Bmul_2992

Alternate gene names: 161526161

Gene position: 3272791-3273777 (Clockwise)

Preceding gene: 161526160

Following gene: 161526162

Centisome position: 94.91

GC content: 64.54

Gene sequence:

>987_bases
ATGAGAACCTCCGCTTCCACTTCGTCCGCGCCGGTGTCGCCCGGCGCCGTGCCGGCTGCCGCGGCGCCGCGCCGCCGCGC
GCGTTTCCCCGCGCTGTCCCGCCTGCTGCCGTCCGGGCGCAGCGTCGCGATCGGCGTGCCGTTCCTGTGGCTCGCAGTCT
TTTTCGCGCTGCCGTTCGTGCTCGTGCTGAAGATCAGCTTCGCCGATCAGGTGATGGGCATTCCGCCGTACACGTCGCTC
GTGCAGGTCAAGGACGGCGTCGTGCACTTCGCGCTGCAGCTGTCGCACTACGCGTTTCTGCTGCAGGACGACCTGTACGT
CGCAACCTATCTCAGCTCGCTGAAGATGGCGGCCGTATCGACGGTGTTCTGCCTGCTGATCGGCTATCCGATGGCGTACT
ACATCGCGCGCTCGGAACCCGGCACGCGCAACGTGCTGATGATGGCCGTGATGCTGCCGTTCTGGACGTCGTTCCTGATT
CGCGTGTACGCGTGGATCGGCATTCTGAAGGACGACGGCCTGCTGAACCACGCGCTGATCGCGCTCGGCATCATCCACAC
GCCGCTGCGGCTCTATCACAGCGATGCGGGCGTCTACATCGGGATGGTCTATTCGTACCTGCCGTTCATGGTGATGCCGC
TGTACGCGCACCTCGTCAAGATGGATCTCACGCTGCTCGAGGCCGCGTACGACCTCGGCGCGAAGCCGTGGGTCGCGTTC
ACGCGCATCACGCTGCCGCTGTCGAAGAACGGCATCATCGCGGGCAGCCTGCTCGTGTTCATTCCGGCGGTGGGCGAATA
CGTGATTCCGGAGCTGCTCGGCGGCGCCGACACGCTGATGATCGGGCGCGTCATGTGGGACGAGTTCTTCAACAACATGG
ACTGGCCGATGGCGTCGGCGGTCACCGTCGCGATGGTGCTGCTGCTGCTCGTGCCGATGGCGTTGTTCCAGTACTACCAG
GTCAAGGAACTGGAGGAAGCGAAATGA

Upstream 100 bases:

>100_bases
TCGCATCTGATGCACGACGGCGCGCCGTCGTGGAACGACGACGTGTTCGTCTCGTGGTCGCCGGCGAGCGGCGTCGTGCT
GACGCAGTGAGGAGCCGACG

Downstream 100 bases:

>100_bases
TCAAGCCGAGCAAACCGCTGTCGACGGGCGTTCTCGCCTTCGGCTTCCTGTTTCTCTACATCCCGATCATCAGCCTCGTC
GTGTACTCGTTCAACGAGTC

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MRTSASTSSAPVSPGAVPAAAAPRRRARFPALSRLLPSGRSVAIGVPFLWLAVFFALPFVLVLKISFADQVMGIPPYTSL
VQVKDGVVHFALQLSHYAFLLQDDLYVATYLSSLKMAAVSTVFCLLIGYPMAYYIARSEPGTRNVLMMAVMLPFWTSFLI
RVYAWIGILKDDGLLNHALIALGIIHTPLRLYHSDAGVYIGMVYSYLPFMVMPLYAHLVKMDLTLLEAAYDLGAKPWVAF
TRITLPLSKNGIIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPMASAVTVAMVLLLLVPMALFQYYQ
VKELEEAK

Sequences:

>Translated_328_residues
MRTSASTSSAPVSPGAVPAAAAPRRRARFPALSRLLPSGRSVAIGVPFLWLAVFFALPFVLVLKISFADQVMGIPPYTSL
VQVKDGVVHFALQLSHYAFLLQDDLYVATYLSSLKMAAVSTVFCLLIGYPMAYYIARSEPGTRNVLMMAVMLPFWTSFLI
RVYAWIGILKDDGLLNHALIALGIIHTPLRLYHSDAGVYIGMVYSYLPFMVMPLYAHLVKMDLTLLEAAYDLGAKPWVAF
TRITLPLSKNGIIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPMASAVTVAMVLLLLVPMALFQYYQ
VKELEEAK
>Mature_328_residues
MRTSASTSSAPVSPGAVPAAAAPRRRARFPALSRLLPSGRSVAIGVPFLWLAVFFALPFVLVLKISFADQVMGIPPYTSL
VQVKDGVVHFALQLSHYAFLLQDDLYVATYLSSLKMAAVSTVFCLLIGYPMAYYIARSEPGTRNVLMMAVMLPFWTSFLI
RVYAWIGILKDDGLLNHALIALGIIHTPLRLYHSDAGVYIGMVYSYLPFMVMPLYAHLVKMDLTLLEAAYDLGAKPWVAF
TRITLPLSKNGIIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPMASAVTVAMVLLLLVPMALFQYYQ
VKELEEAK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=296, Percent_Identity=55.0675675675676, Blast_Score=318, Evalue=3e-88,
Organism=Escherichia coli, GI1787369, Length=279, Percent_Identity=39.0681003584229, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1787713, Length=213, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36211; Mature: 36211

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSASTSSAPVSPGAVPAAAAPRRRARFPALSRLLPSGRSVAIGVPFLWLAVFFALPFV
CCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHCCCCCEEEECHHHHHHHHHHHHHHH
LVLKISFADQVMGIPPYTSLVQVKDGVVHFALQLSHYAFLLQDDLYVATYLSSLKMAAVS
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
TVFCLLIGYPMAYYIARSEPGTRNVLMMAVMLPFWTSFLIRVYAWIGILKDDGLLNHALI
HHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ALGIIHTPLRLYHSDAGVYIGMVYSYLPFMVMPLYAHLVKMDLTLLEAAYDLGAKPWVAF
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
TRITLPLSKNGIIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPMASA
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHH
VTVAMVLLLLVPMALFQYYQVKELEEAK
HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRTSASTSSAPVSPGAVPAAAAPRRRARFPALSRLLPSGRSVAIGVPFLWLAVFFALPFV
CCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHCCCCCEEEECHHHHHHHHHHHHHHH
LVLKISFADQVMGIPPYTSLVQVKDGVVHFALQLSHYAFLLQDDLYVATYLSSLKMAAVS
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
TVFCLLIGYPMAYYIARSEPGTRNVLMMAVMLPFWTSFLIRVYAWIGILKDDGLLNHALI
HHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ALGIIHTPLRLYHSDAGVYIGMVYSYLPFMVMPLYAHLVKMDLTLLEAAYDLGAKPWVAF
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
TRITLPLSKNGIIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPMASA
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHH
VTVAMVLLLLVPMALFQYYQVKELEEAK
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]