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The map label for this gene is tapB [H]

Identifier: 161525987

GI number: 161525987

Start: 3070493

End: 3071860

Strand: Direct

Name: tapB [H]

Synonym: Bmul_2818

Alternate gene names: 161525987

Gene position: 3070493-3071860 (Clockwise)

Preceding gene: 161525986

Following gene: 161525988

Centisome position: 89.04

GC content: 71.78

Gene sequence:

>1368_bases
ATGGGCGCGGCCGCGCCTGACCGAACGGCCGACTACCGGCGCCTGCCGTGCCGCGCGATCCTGTACGCGTCACGATCAAC
GGCGGCCGACGCGCCGGCTCACATGAACTTTCCCGCTTCCCGCGCGGCGCCGCGTGCGCCTGCGCCGTCCGACGCAGCGG
CCGCTGCGCCCCGCTCGCTCGATGCGGCGCAGCTCGACGATGCGCCTGCGGTGCGGCTGCTCACCGACACGCTGCATGCG
GCGCATGCACGCGATGCGTCCGACATTCATATCGAGCCGTTCGAAGGCGGCTGGCGCATCCGGCTGCGCATCGACGGCGT
GCTGCACGAGCATGGGCGGCCGCCCGTTCACCTGCGCGACGCGCTTGTCACGCGCATCAAGGTGCTCGCGCGCATGGACA
TCGCCGAGCGCCGGCTTCCGCAGGACGGCCGGCTGCGGATCGCGCTCGGCGGCGGCACGCGCGGCGACTACCGCGTGAGC
TCGCTGCCGACGCTGTTCGGCGAAAAGCTCGTGCTGCGCCGGCTCGAAACGCTGCCGCCCGATCTCACGCTTGCCGCGCT
CGGCTTCGATGCGCAGCAGGCACGCGCGATGGAAGCGGCGATCCGCGCACCGCACGGCCTCGTGCTCGTCACGGGCCCGA
CCGGCAGCGGCAAGACACTGTCGCTGTACTGCGCGCTGCAGATGCTCGATCGCGATGCCCACAACGTCTGCACGGTCGAA
GATCCGGCCGAAATCCAGCTCGACGGCATCAACCAGGTCGGCGTCGCGGAAAAAGCCGGCCTCACGTTTGCGACCGCGCT
GCGCGCGCTGCTGCGGCAGGATCCGGACGTCATCATGGTCGGCGAGATCCGCGATGCCGAAACGGCCGACGTCGCCATCA
AGGCCGCGCAGACCGGCCATCTCGTGCTGTCGACGCTGCATACGAACGACGCGCCGGCCGCGATCGCACGGCTGCTCGAC
ATCGGCGTCGCGCCGTACAACCTCGCGGCCGCGCTGCGCCTCGTCACGGCGCAACGGCTCGTCAGGCGGCTGTGCATCGG
CTGCCTCGAGCCGTCGGACGCGCCAGCCGCGCTGCTGCGTGACGCCGGCTGCGACGCGCCCGCGCTCGAATCGGGATGGC
GCCCGTTCGTCGCGCGCGGCTGCGCCGCATGTCATGGCATCGGCTATCGCGGCCGCATCGGCCTGCATCAGACGATGCCG
GTGTCGTCGCGGCTCGCCGAATGTATCGTCGCGCGTGCGAGCGTCGGCGAACTCGCGCGATGCGCGGCGGCGGAAGGCGT
TCGCACTTTGCGTGACGCGGCGCTCGCGCGCGTTCGAGACGGCACGACGAGCGTCGCGGAAGCGCTCGCCGCCACGCCGA
ACGCATGA

Upstream 100 bases:

>100_bases
GGCGGCGTGCCCGGCGCCCCGCCCCGCGCGCCACTGCGATGCGTCAGAAGAACGGGTTTCACCGTCACGCCAACCTCCCC
GCTAACGCCGCACGCCCGTC

Downstream 100 bases:

>100_bases
CTATGACCATGCGCGCGCGGCCTCGCGAGATGCGCTTTGCCTGGCGCGGCCGTCGCCGCGACGGCACGCCGCGCAGCGGT
ACCGTCGTCGCATTCGACGC

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MGAAAPDRTADYRRLPCRAILYASRSTAADAPAHMNFPASRAAPRAPAPSDAAAAAPRSLDAAQLDDAPAVRLLTDTLHA
AHARDASDIHIEPFEGGWRIRLRIDGVLHEHGRPPVHLRDALVTRIKVLARMDIAERRLPQDGRLRIALGGGTRGDYRVS
SLPTLFGEKLVLRRLETLPPDLTLAALGFDAQQARAMEAAIRAPHGLVLVTGPTGSGKTLSLYCALQMLDRDAHNVCTVE
DPAEIQLDGINQVGVAEKAGLTFATALRALLRQDPDVIMVGEIRDAETADVAIKAAQTGHLVLSTLHTNDAPAAIARLLD
IGVAPYNLAAALRLVTAQRLVRRLCIGCLEPSDAPAALLRDAGCDAPALESGWRPFVARGCAACHGIGYRGRIGLHQTMP
VSSRLAECIVARASVGELARCAAAEGVRTLRDAALARVRDGTTSVAEALAATPNA

Sequences:

>Translated_455_residues
MGAAAPDRTADYRRLPCRAILYASRSTAADAPAHMNFPASRAAPRAPAPSDAAAAAPRSLDAAQLDDAPAVRLLTDTLHA
AHARDASDIHIEPFEGGWRIRLRIDGVLHEHGRPPVHLRDALVTRIKVLARMDIAERRLPQDGRLRIALGGGTRGDYRVS
SLPTLFGEKLVLRRLETLPPDLTLAALGFDAQQARAMEAAIRAPHGLVLVTGPTGSGKTLSLYCALQMLDRDAHNVCTVE
DPAEIQLDGINQVGVAEKAGLTFATALRALLRQDPDVIMVGEIRDAETADVAIKAAQTGHLVLSTLHTNDAPAAIARLLD
IGVAPYNLAAALRLVTAQRLVRRLCIGCLEPSDAPAALLRDAGCDAPALESGWRPFVARGCAACHGIGYRGRIGLHQTMP
VSSRLAECIVARASVGELARCAAAEGVRTLRDAALARVRDGTTSVAEALAATPNA
>Mature_454_residues
GAAAPDRTADYRRLPCRAILYASRSTAADAPAHMNFPASRAAPRAPAPSDAAAAAPRSLDAAQLDDAPAVRLLTDTLHAA
HARDASDIHIEPFEGGWRIRLRIDGVLHEHGRPPVHLRDALVTRIKVLARMDIAERRLPQDGRLRIALGGGTRGDYRVSS
LPTLFGEKLVLRRLETLPPDLTLAALGFDAQQARAMEAAIRAPHGLVLVTGPTGSGKTLSLYCALQMLDRDAHNVCTVED
PAEIQLDGINQVGVAEKAGLTFATALRALLRQDPDVIMVGEIRDAETADVAIKAAQTGHLVLSTLHTNDAPAAIARLLDI
GVAPYNLAAALRLVTAQRLVRRLCIGCLEPSDAPAALLRDAGCDAPALESGWRPFVARGCAACHGIGYRGRIGLHQTMPV
SSRLAECIVARASVGELARCAAAEGVRTLRDAALARVRDGTTSVAEALAATPNA

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=388, Percent_Identity=46.6494845360825, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1789723, Length=383, Percent_Identity=44.9086161879896, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI87082188, Length=268, Percent_Identity=35.0746268656716, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 48111; Mature: 47980

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAAAPDRTADYRRLPCRAILYASRSTAADAPAHMNFPASRAAPRAPAPSDAAAAAPRSL
CCCCCCCCCCCHHHCCCCEEEEECCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHCCCCCC
DAAQLDDAPAVRLLTDTLHAAHARDASDIHIEPFEGGWRIRLRIDGVLHEHGRPPVHLRD
CHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEEEEHHHCCCCCHHHHH
ALVTRIKVLARMDIAERRLPQDGRLRIALGGGTRGDYRVSSLPTLFGEKLVLRRLETLPP
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEHHHCHHHHHHHHHHHHHHHCCC
DLTLAALGFDAQQARAMEAAIRAPHGLVLVTGPTGSGKTLSLYCALQMLDRDAHNVCTVE
CCEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEHHHHHHHHCCCCCCEEECC
DPAEIQLDGINQVGVAEKAGLTFATALRALLRQDPDVIMVGEIRDAETADVAIKAAQTGH
CCCEEEECCCCCCCCHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCC
LVLSTLHTNDAPAAIARLLDIGVAPYNLAAALRLVTAQRLVRRLCIGCLEPSDAPAALLR
EEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
DAGCDAPALESGWRPFVARGCAACHGIGYRGRIGLHQTMPVSSRLAECIVARASVGELAR
HCCCCCCCHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
CAAAEGVRTLRDAALARVRDGTTSVAEALAATPNA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
GAAAPDRTADYRRLPCRAILYASRSTAADAPAHMNFPASRAAPRAPAPSDAAAAAPRSL
CCCCCCCCCCHHHCCCCEEEEECCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHCCCCCC
DAAQLDDAPAVRLLTDTLHAAHARDASDIHIEPFEGGWRIRLRIDGVLHEHGRPPVHLRD
CHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEEEEHHHCCCCCHHHHH
ALVTRIKVLARMDIAERRLPQDGRLRIALGGGTRGDYRVSSLPTLFGEKLVLRRLETLPP
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEHHHCHHHHHHHHHHHHHHHCCC
DLTLAALGFDAQQARAMEAAIRAPHGLVLVTGPTGSGKTLSLYCALQMLDRDAHNVCTVE
CCEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEHHHHHHHHCCCCCCEEECC
DPAEIQLDGINQVGVAEKAGLTFATALRALLRQDPDVIMVGEIRDAETADVAIKAAQTGH
CCCEEEECCCCCCCCHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCC
LVLSTLHTNDAPAAIARLLDIGVAPYNLAAALRLVTAQRLVRRLCIGCLEPSDAPAALLR
EEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
DAGCDAPALESGWRPFVARGCAACHGIGYRGRIGLHQTMPVSSRLAECIVARASVGELAR
HCCCCCCCHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
CAAAEGVRTLRDAALARVRDGTTSVAEALAATPNA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]