BacMap

Definition	Burkholderia multivorans ATCC 17616 chromosome chromosome 1, complete sequence.
Accession	NC_010084
Length	3,448,466

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The map label for this gene is cpnA [H]

Identifier: 161525930

GI number: 161525930

Start: 3013315

End: 3014109

Strand: Reverse

Name: cpnA [H]

Synonym: Bmul_2761

Alternate gene names: 161525930

Gene position: 3014109-3013315 (Counterclockwise)

Preceding gene: 161525931

Following gene: 161525929

Centisome position: 87.4

GC content: 70.06

Gene sequence:

>795_bases
ATGACCACCGATTCTTCCGCGCGCGACGCGCATGCGAACTATGCGCACTATCCGAGCCTCGTCGATCGCGCGGTGCTGAT
CACCGGCGGTGCGACCGGCATCGGCGCGTCGTTCGTCGAGCACTTCGCGCAGCAGGGCGCGCGCGTCGCGTTCGTCGACC
TCGATGCGGACGCGGGCCGCGCGCTCGCCGATCGTCTCGGCGGCGCACGTCACGCGCCGCTGTTCGTGCCGTGCGACCTG
ACCGACATCGAGGCGCTGCGTCGCGCGATCGACACGATCCGCACGCAGATCGGCGCGATCGCGGTGCTCGTGAACAATGC
GGCGAACGACGTGCGGCATGCGATCGCCGACGTGACGCCCGCGTCGTTCGACGCCGGCATCGCGGTGAACCTGCGGCACC
AGTTCTTCGCCGCGCAGGCCGTGATCGACGACATGAAGCGGCTCGGCGGCGGCGCGATCATCAACCTCGGCTCGATCAGC
TGGATGCTGAAGAACGGCGGCTATCCGGTGTATGTGATGGCGAAGGCGGCCGTGCAGGGCCTCACGCGCGGGCTCGCGCG
CGATCTCGGCCCGTTCGGGATCCGCGTGAATTCGCTGGTGCCCGGCTGGGTGATGACCGACAAGCAGCGCCGGCTCTGGC
TCGACGACGCGGGCCGCGCGGCGATCAAGGCCGGCCAGTGCCTCGACGCGGAGCTGCTGCCGGCCGATCTCGCACGCATG
GCGCTGTTCCTCGCGGCCGACGACAGCCGGATGATCACCGCACAGGACGTGATCGTCGACGGCGGGTGGGCCTGA

Upstream 100 bases:

>100_bases
CAGTATCTCGTGCGCGGCGCGATCCTGCTCGCGGCCGTGCTGCTCGACCAGCTGAAGAACCGCGGCGCACGCGACTGAGC
CGCCTACACGGAGACGCTGC

Downstream 100 bases:

>100_bases
CCGTCGCGCGGCCCGCGTAGCGGCCGCCCATCGAGCGCACCGTTTCTTCGACGAACGAAAGGAGCTGATCATGACCGCCA
CGTCCTCGCGCGCATCGTCG

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTTDSSARDAHANYAHYPSLVDRAVLITGGATGIGASFVEHFAQQGARVAFVDLDADAGRALADRLGGARHAPLFVPCDL
TDIEALRRAIDTIRTQIGAIAVLVNNAANDVRHAIADVTPASFDAGIAVNLRHQFFAAQAVIDDMKRLGGGAIINLGSIS
WMLKNGGYPVYVMAKAAVQGLTRGLARDLGPFGIRVNSLVPGWVMTDKQRRLWLDDAGRAAIKAGQCLDAELLPADLARM
ALFLAADDSRMITAQDVIVDGGWA

Sequences:

>Translated_264_residues
MTTDSSARDAHANYAHYPSLVDRAVLITGGATGIGASFVEHFAQQGARVAFVDLDADAGRALADRLGGARHAPLFVPCDL
TDIEALRRAIDTIRTQIGAIAVLVNNAANDVRHAIADVTPASFDAGIAVNLRHQFFAAQAVIDDMKRLGGGAIINLGSIS
WMLKNGGYPVYVMAKAAVQGLTRGLARDLGPFGIRVNSLVPGWVMTDKQRRLWLDDAGRAAIKAGQCLDAELLPADLARM
ALFLAADDSRMITAQDVIVDGGWA
>Mature_263_residues
TTDSSARDAHANYAHYPSLVDRAVLITGGATGIGASFVEHFAQQGARVAFVDLDADAGRALADRLGGARHAPLFVPCDLT
DIEALRRAIDTIRTQIGAIAVLVNNAANDVRHAIADVTPASFDAGIAVNLRHQFFAAQAVIDDMKRLGGGAIINLGSISW
MLKNGGYPVYVMAKAAVQGLTRGLARDLGPFGIRVNSLVPGWVMTDKQRRLWLDDAGRAAIKAGQCLDAELLPADLARMA
LFLAADDSRMITAQDVIVDGGWA

Specific function: Reduces cyclopentanol to cyclopentanone. The enzyme also shows considerable activity toward cyclohexanol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=259, Percent_Identity=30.1158301158301, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI7705925, Length=253, Percent_Identity=34.3873517786561, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI15277342, Length=251, Percent_Identity=32.6693227091633, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI40254992, Length=243, Percent_Identity=26.7489711934156, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI126723750, Length=262, Percent_Identity=25.1908396946565, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI59889578, Length=233, Percent_Identity=29.6137339055794, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI31542939, Length=193, Percent_Identity=30.5699481865285, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI19923817, Length=259, Percent_Identity=26.2548262548263, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI10190704, Length=269, Percent_Identity=29.368029739777, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI66933014, Length=252, Percent_Identity=29.3650793650794, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI4758504, Length=193, Percent_Identity=31.6062176165803, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI126723191, Length=218, Percent_Identity=27.0642201834862, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI224922801, Length=171, Percent_Identity=29.2397660818713, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI83715985, Length=181, Percent_Identity=30.3867403314917, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1787335, Length=240, Percent_Identity=31.6666666666667, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790717, Length=244, Percent_Identity=29.9180327868852, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87082100, Length=251, Percent_Identity=29.8804780876494, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1787905, Length=249, Percent_Identity=30.9236947791165, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1789208, Length=251, Percent_Identity=27.8884462151394, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI87082160, Length=257, Percent_Identity=28.0155642023346, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI2367365, Length=247, Percent_Identity=29.5546558704453, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789378, Length=256, Percent_Identity=29.6875, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786812, Length=250, Percent_Identity=29.2, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789057, Length=256, Percent_Identity=31.25, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788891, Length=193, Percent_Identity=32.1243523316062, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI2367175, Length=188, Percent_Identity=28.1914893617021, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=255, Percent_Identity=31.3725490196078, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17555706, Length=246, Percent_Identity=28.8617886178862, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI25147288, Length=250, Percent_Identity=29.2, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17562904, Length=265, Percent_Identity=29.4339622641509, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI72000259, Length=266, Percent_Identity=29.3233082706767, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560332, Length=272, Percent_Identity=29.4117647058824, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71994600, Length=245, Percent_Identity=27.7551020408163, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17563726, Length=262, Percent_Identity=28.2442748091603, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17560150, Length=262, Percent_Identity=29.7709923664122, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17559104, Length=271, Percent_Identity=28.7822878228782, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538480, Length=276, Percent_Identity=29.7101449275362, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17561402, Length=257, Percent_Identity=28.7937743190661, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17562910, Length=271, Percent_Identity=27.3062730627306, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17560220, Length=268, Percent_Identity=29.4776119402985, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71994604, Length=244, Percent_Identity=25.4098360655738, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562990, Length=273, Percent_Identity=29.6703296703297, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17508651, Length=247, Percent_Identity=26.7206477732794, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17551412, Length=269, Percent_Identity=30.1115241635688, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17538182, Length=202, Percent_Identity=29.2079207920792, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562906, Length=256, Percent_Identity=26.5625, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17544670, Length=266, Percent_Identity=27.4436090225564, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=256, Percent_Identity=26.5625, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17567345, Length=187, Percent_Identity=25.1336898395722, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI193204405, Length=264, Percent_Identity=28.4090909090909, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17565030, Length=273, Percent_Identity=28.2051282051282, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17531453, Length=272, Percent_Identity=27.2058823529412, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=198, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6324126, Length=251, Percent_Identity=25.4980079681275, Blast_Score=62, Evalue=9e-11,
Organism=Drosophila melanogaster, GI21357041, Length=250, Percent_Identity=30, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI28571526, Length=252, Percent_Identity=29.3650793650794, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24643142, Length=245, Percent_Identity=28.1632653061224, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24639444, Length=256, Percent_Identity=28.515625, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI23397609, Length=252, Percent_Identity=28.968253968254, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24644337, Length=247, Percent_Identity=31.1740890688259, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24644339, Length=245, Percent_Identity=26.9387755102041, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21355319, Length=252, Percent_Identity=24.2063492063492, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17737361, Length=206, Percent_Identity=30.5825242718447, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281364892, Length=182, Percent_Identity=29.6703296703297, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.163 [H]

Molecular weight: Translated: 27958; Mature: 27827

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDSSARDAHANYAHYPSLVDRAVLITGGATGIGASFVEHFAQQGARVAFVDLDADAGR
CCCCCCCCCCCCCCHHCHHHHCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCH
ALADRLGGARHAPLFVPCDLTDIEALRRAIDTIRTQIGAIAVLVNNAANDVRHAIADVTP
HHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHCCC
ASFDAGIAVNLRHQFFAAQAVIDDMKRLGGGAIINLGSISWMLKNGGYPVYVMAKAAVQG
CCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEECCCCEEEEEHHHHHHH
LTRGLARDLGPFGIRVNSLVPGWVMTDKQRRLWLDDAGRAAIKAGQCLDAELLPADLARM
HHHHHHHCCCCCCEEECCCCCCCEEECCCCEEEECCCCCHHHHCCCCCCCCCCHHHHHHH
ALFLAADDSRMITAQDVIVDGGWA
EEHEECCCCCEEEEEEEEECCCCC
>Mature Secondary Structure 
TTDSSARDAHANYAHYPSLVDRAVLITGGATGIGASFVEHFAQQGARVAFVDLDADAGR
CCCCCCCCCCCCCHHCHHHHCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCH
ALADRLGGARHAPLFVPCDLTDIEALRRAIDTIRTQIGAIAVLVNNAANDVRHAIADVTP
HHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHCCC
ASFDAGIAVNLRHQFFAAQAVIDDMKRLGGGAIINLGSISWMLKNGGYPVYVMAKAAVQG
CCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEECCCCEEEEEHHHHHHH
LTRGLARDLGPFGIRVNSLVPGWVMTDKQRRLWLDDAGRAAIKAGQCLDAELLPADLARM
HHHHHHHCCCCCCEEECCCCCCCEEECCCCEEEECCCCCHHHHCCCCCCCCCCHHHHHHH
ALFLAADDSRMITAQDVIVDGGWA
EEHEECCCCCEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12406764 [H]