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The map label for this gene is speE [H]

Identifier: 161525918

GI number: 161525918

Start: 3001788

End: 3002675

Strand: Direct

Name: speE [H]

Synonym: Bmul_2749

Alternate gene names: 161525918

Gene position: 3001788-3002675 (Clockwise)

Preceding gene: 161525914

Following gene: 161525919

Centisome position: 87.05

GC content: 69.14

Gene sequence:

>888_bases
TTGGAACTGCCCGCCTCGCGCGGCCCGCCCGTGAGCACAACCCTCCTCTTTCATCCGACGCCCGACGCCACCTACGGCTT
TCCGAATGCGCGCCGCCTGGCGCGCGTCGCGTCCGCGCACCAGCAGATCGAAGTCTGGGAAACCGCGCAGCTCGGCCGGC
TGTTCACGCTCGACGGGCGGCCGATGACGTCCGTCGGCGACGAGTACGTCTACCACGAGTGCATGACGCATCCGGCCGCG
CTCGCGCATCCGGCGCCGAAAAAGGCGCTCGTACTCGGTGGCGGCGACGGCGGCGCCGCGCGCCAGCTGCTCAAGCACGC
ATGCATCGAACGGATCGTCGTCGCCGAGCTCGACGACGAAGTCGTCGGCATGGCGCGCCGCTATCTCGACGACGTCCATC
AAGGCGCGCTCGACGATCCGCGCGTCGAACTCGTGATCGGCGATGCCGCGCATTTCGTCGAATCGACGGTCGAGCATTTC
GATCTCGTCGTGTTCGATCTGACGCCGCCCGATTCGCCGGCCGCCGGCCTCTACACGCGCGCCTTCTACGCACGGCTCAA
GCGCATCCTGACGCCGCGCGGCGCGGTGTCGATGCATCTCGGCTCGCCGCTCTTCCATGCAACGCGGATCGCCGCGCTGC
TCGAGGATTTGCGCGCGAGCTTCGCGGTCGTCGATCCACTCTCCGCGCACGTGCCGCTGTACGGCTCGCAATGGCTGATG
GCGATCGCGAGCGACACGCTCGACGCGGCCGCGCTGTTCGCGCACGACATCGACGAACGGCTGGCCGCGCGCCGCATCGA
AGGCCTGCGCTACTACGATGCGCGGCTGCATGCGGCCCTTTTTGCGCTGCCGCGCGCGCTGCGCGATACACTGGGCGCTC
GCCGCTGA

Upstream 100 bases:

>100_bases
AAAGCTCGTCATTTTAACAGATGGGCCGGGATTTCCCCGGCGCATCGGCTTGTTCCGGCACGGTTTTCCGCGAAAGCGCG
CGCCTCCTATAATTCAGGCG

Downstream 100 bases:

>100_bases
GCGTACGCTTCGCACGCGCGTGCCGTGCTCGCCGCTTCCCGCGGTGCGCGAACGCGCGCAGCGAACCGGCGCACGCTCGC
GCCGCCTCCCCCGAACGACA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MELPASRGPPVSTTLLFHPTPDATYGFPNARRLARVASAHQQIEVWETAQLGRLFTLDGRPMTSVGDEYVYHECMTHPAA
LAHPAPKKALVLGGGDGGAARQLLKHACIERIVVAELDDEVVGMARRYLDDVHQGALDDPRVELVIGDAAHFVESTVEHF
DLVVFDLTPPDSPAAGLYTRAFYARLKRILTPRGAVSMHLGSPLFHATRIAALLEDLRASFAVVDPLSAHVPLYGSQWLM
AIASDTLDAAALFAHDIDERLAARRIEGLRYYDARLHAALFALPRALRDTLGARR

Sequences:

>Translated_295_residues
MELPASRGPPVSTTLLFHPTPDATYGFPNARRLARVASAHQQIEVWETAQLGRLFTLDGRPMTSVGDEYVYHECMTHPAA
LAHPAPKKALVLGGGDGGAARQLLKHACIERIVVAELDDEVVGMARRYLDDVHQGALDDPRVELVIGDAAHFVESTVEHF
DLVVFDLTPPDSPAAGLYTRAFYARLKRILTPRGAVSMHLGSPLFHATRIAALLEDLRASFAVVDPLSAHVPLYGSQWLM
AIASDTLDAAALFAHDIDERLAARRIEGLRYYDARLHAALFALPRALRDTLGARR
>Mature_295_residues
MELPASRGPPVSTTLLFHPTPDATYGFPNARRLARVASAHQQIEVWETAQLGRLFTLDGRPMTSVGDEYVYHECMTHPAA
LAHPAPKKALVLGGGDGGAARQLLKHACIERIVVAELDDEVVGMARRYLDDVHQGALDDPRVELVIGDAAHFVESTVEHF
DLVVFDLTPPDSPAAGLYTRAFYARLKRILTPRGAVSMHLGSPLFHATRIAALLEDLRASFAVVDPLSAHVPLYGSQWLM
AIASDTLDAAALFAHDIDERLAARRIEGLRYYDARLHAALFALPRALRDTLGARR

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=295, Percent_Identity=25.7627118644068, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1786312, Length=281, Percent_Identity=35.2313167259786, Blast_Score=165, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI25154106, Length=278, Percent_Identity=29.4964028776978, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI212645616, Length=323, Percent_Identity=25.3869969040248, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6323175, Length=262, Percent_Identity=31.2977099236641, Blast_Score=141, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6325326, Length=250, Percent_Identity=29.2, Blast_Score=129, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24645443, Length=270, Percent_Identity=30.3703703703704, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI21355941, Length=210, Percent_Identity=31.9047619047619, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 32277; Mature: 32277

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELPASRGPPVSTTLLFHPTPDATYGFPNARRLARVASAHQQIEVWETAQLGRLFTLDGR
CCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
PMTSVGDEYVYHECMTHPAALAHPAPKKALVLGGGDGGAARQLLKHACIERIVVAELDDE
CCCCCCHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHH
VVGMARRYLDDVHQGALDDPRVELVIGDAAHFVESTVEHFDLVVFDLTPPDSPAAGLYTR
HHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHH
AFYARLKRILTPRGAVSMHLGSPLFHATRIAALLEDLRASFAVVDPLSAHVPLYGSQWLM
HHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
AIASDTLDAAALFAHDIDERLAARRIEGLRYYDARLHAALFALPRALRDTLGARR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MELPASRGPPVSTTLLFHPTPDATYGFPNARRLARVASAHQQIEVWETAQLGRLFTLDGR
CCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
PMTSVGDEYVYHECMTHPAALAHPAPKKALVLGGGDGGAARQLLKHACIERIVVAELDDE
CCCCCCHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHH
VVGMARRYLDDVHQGALDDPRVELVIGDAAHFVESTVEHFDLVVFDLTPPDSPAAGLYTR
HHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHH
AFYARLKRILTPRGAVSMHLGSPLFHATRIAALLEDLRASFAVVDPLSAHVPLYGSQWLM
HHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
AIASDTLDAAALFAHDIDERLAARRIEGLRYYDARLHAALFALPRALRDTLGARR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]