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The map label for this gene is tyrS [H]

Identifier: 161525869

GI number: 161525869

Start: 2947464

End: 2948828

Strand: Reverse

Name: tyrS [H]

Synonym: Bmul_2700

Alternate gene names: 161525869

Gene position: 2948828-2947464 (Counterclockwise)

Preceding gene: 161525871

Following gene: 161525868

Centisome position: 85.51

GC content: 64.69

Gene sequence:

>1365_bases
ATGTCGCGCGGGCACGATCCGCGCGCGTTGCGCCGATTATCCGCGCAACGCGGCGCGCCGTTAAGCGACGCGCGCGCAAG
TTATGGGAAAATCCCTGTTTTTGCGACATTCTTCCTGGCACCGATGAGCACCGAACCCAGTTCCAAGCCCGTTTTCCCGA
TCACCGACGAAGTCCGGCATGCGCTCGCCGTCACGAAGCGCGGCGTCGACGAGCTGCTGATCGAGGAAGAGTTCGCGCAG
AAGCTCGCGCGCAGCGCGGCCACCGGCAAGCCGCTGCGCATCAAGCTCGGCCTCGACCCGACGGCGCCCGACATCCATAT
CGGCCACACGGTCGTGCTGAACAAGATGCGTCAGCTGCAGGATCTCGGCCATACGGTGATCTTCCTGATCGGCGATTTCA
CGTCGCTGATCGGCGATCCGTCGGGCCGCAACGCGACGCGTCCGCCGCTCACGCGCGAGCAGATCGAGGCGAACGCGAAG
ACCTACTTCGAGCAGGCTGCGCTCGTGCTCGACCGCGACAAGACCGAGATCCGCTACAACAGCGAATGGTCGATGCCGCT
CGGTGCGGACGGGATGATCAAGCTCGCGTCGCGCTACACGGTCGCGCGCATTCTCGAGCGCGAGGACTTCACGAAGCGCT
TCCAGGGCGGCGTGCCGATCTCGATCCACGAATTCCTGTACCCGCTGATGCAGGGCTACGACTCGGTCGCGCTGAACGCC
GATCTCGAGCTCGGCGGCACCGACCAGAAGTTCAACCTGCTGGTCGGCCGCGAACTGCAGAAGCAGTACGGCCAGGAACA
GCAGTGCATCCTGACGATGCCGCTGCTCGAAGGGCTCGACGGCGTCGAGAAGATGTCGAAGTCGAAGGGCAACTACGTCG
GCATCAGCGAGAAACCGACCGATATGTTCGGCAAGCTGATGAGCATCTCCGACACGCTGATGTGGCGTTACTTCGAACTG
CTGTCGTTCCGCAGCATGGACGAGATCGCCGGCTTCAAGCGCGAAGCCGAGGGCGGCCGCAATCCGCGCGACTTCAAGGT
GATGCTCGCGCAGGAAATCGTCGCGCGCTTCCATTCGCAGGCCGATGCCGAGCGTGCGCTGGAAGACTTCAACCATCGCG
CGAAGGGCGGTGTGCCGGACGACATTCCGTCGGTCTCGCTCGCCGGTGCGCCGCTCGCGATCGGCCAGCTGCTGAAGCAG
GCGGGGCTCGTGCCGTCGACGAGCGAAGCGCTGCGCAACATCGAGCAGGGCGGCGTGAAGATCGACGGCACGACGGTGTC
CGACAAGGGCCTGAAGGTCGACGCCGGCGAATACGTCGTGCAGGTCGGCAAGCGGCGTTTCGCGCGCGTGACGCTCGCTG
CATGA

Upstream 100 bases:

>100_bases
GCCGTCCATGCTGGTGCCGGACATCAGGCCGAAGTAGATGCCGTCGGCCGGATGGGCGGTTGGCGGATGTCGTTGCGGCA
CGTTGCGGTTCTCCTGAAGC

Downstream 100 bases:

>100_bases
TCGCGCTGATCCAGCGCGTGAAGCGCGCCGACGTGCGCGTCGGCGAGCGCGTGACGGGCGAGATCGGCGCCGGCCTGCTT
GCGCTCGTGTGTGCTGAGCG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSRGHDPRALRRLSAQRGAPLSDARASYGKIPVFATFFLAPMSTEPSSKPVFPITDEVRHALAVTKRGVDELLIEEEFAQ
KLARSAATGKPLRIKLGLDPTAPDIHIGHTVVLNKMRQLQDLGHTVIFLIGDFTSLIGDPSGRNATRPPLTREQIEANAK
TYFEQAALVLDRDKTEIRYNSEWSMPLGADGMIKLASRYTVARILEREDFTKRFQGGVPISIHEFLYPLMQGYDSVALNA
DLELGGTDQKFNLLVGRELQKQYGQEQQCILTMPLLEGLDGVEKMSKSKGNYVGISEKPTDMFGKLMSISDTLMWRYFEL
LSFRSMDEIAGFKREAEGGRNPRDFKVMLAQEIVARFHSQADAERALEDFNHRAKGGVPDDIPSVSLAGAPLAIGQLLKQ
AGLVPSTSEALRNIEQGGVKIDGTTVSDKGLKVDAGEYVVQVGKRRFARVTLAA

Sequences:

>Translated_454_residues
MSRGHDPRALRRLSAQRGAPLSDARASYGKIPVFATFFLAPMSTEPSSKPVFPITDEVRHALAVTKRGVDELLIEEEFAQ
KLARSAATGKPLRIKLGLDPTAPDIHIGHTVVLNKMRQLQDLGHTVIFLIGDFTSLIGDPSGRNATRPPLTREQIEANAK
TYFEQAALVLDRDKTEIRYNSEWSMPLGADGMIKLASRYTVARILEREDFTKRFQGGVPISIHEFLYPLMQGYDSVALNA
DLELGGTDQKFNLLVGRELQKQYGQEQQCILTMPLLEGLDGVEKMSKSKGNYVGISEKPTDMFGKLMSISDTLMWRYFEL
LSFRSMDEIAGFKREAEGGRNPRDFKVMLAQEIVARFHSQADAERALEDFNHRAKGGVPDDIPSVSLAGAPLAIGQLLKQ
AGLVPSTSEALRNIEQGGVKIDGTTVSDKGLKVDAGEYVVQVGKRRFARVTLAA
>Mature_453_residues
SRGHDPRALRRLSAQRGAPLSDARASYGKIPVFATFFLAPMSTEPSSKPVFPITDEVRHALAVTKRGVDELLIEEEFAQK
LARSAATGKPLRIKLGLDPTAPDIHIGHTVVLNKMRQLQDLGHTVIFLIGDFTSLIGDPSGRNATRPPLTREQIEANAKT
YFEQAALVLDRDKTEIRYNSEWSMPLGADGMIKLASRYTVARILEREDFTKRFQGGVPISIHEFLYPLMQGYDSVALNAD
LELGGTDQKFNLLVGRELQKQYGQEQQCILTMPLLEGLDGVEKMSKSKGNYVGISEKPTDMFGKLMSISDTLMWRYFELL
SFRSMDEIAGFKREAEGGRNPRDFKVMLAQEIVARFHSQADAERALEDFNHRAKGGVPDDIPSVSLAGAPLAIGQLLKQA
GLVPSTSEALRNIEQGGVKIDGTTVSDKGLKVDAGEYVVQVGKRRFARVTLAA

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=398, Percent_Identity=25.8793969849246, Blast_Score=102, Evalue=9e-22,
Organism=Escherichia coli, GI1787925, Length=424, Percent_Identity=29.7169811320755, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25145802, Length=210, Percent_Identity=29.0476190476191, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6325160, Length=370, Percent_Identity=24.5945945945946, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20130383, Length=450, Percent_Identity=24.8888888888889, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 50119; Mature: 49988

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRGHDPRALRRLSAQRGAPLSDARASYGKIPVFATFFLAPMSTEPSSKPVFPITDEVRH
CCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
ALAVTKRGVDELLIEEEFAQKLARSAATGKPLRIKLGLDPTAPDIHIGHTVVLNKMRQLQ
HHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHH
DLGHTVIFLIGDFTSLIGDPSGRNATRPPLTREQIEANAKTYFEQAALVLDRDKTEIRYN
HCCCEEEEEHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCEEEEC
SEWSMPLGADGMIKLASRYTVARILEREDFTKRFQGGVPISIHEFLYPLMQGYDSVALNA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCEEEEC
DLELGGTDQKFNLLVGRELQKQYGQEQQCILTMPLLEGLDGVEKMSKSKGNYVGISEKPT
CEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHCCHHHHHHHHCCCCEECCCCCCH
DMFGKLMSISDTLMWRYFELLSFRSMDEIAGFKREAEGGRNPRDFKVMLAQEIVARFHSQ
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
ADAERALEDFNHRAKGGVPDDIPSVSLAGAPLAIGQLLKQAGLVPSTSEALRNIEQGGVK
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCEE
IDGTTVSDKGLKVDAGEYVVQVGKRRFARVTLAA
ECCEEECCCCCEECHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure 
SRGHDPRALRRLSAQRGAPLSDARASYGKIPVFATFFLAPMSTEPSSKPVFPITDEVRH
CCCCCHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
ALAVTKRGVDELLIEEEFAQKLARSAATGKPLRIKLGLDPTAPDIHIGHTVVLNKMRQLQ
HHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHH
DLGHTVIFLIGDFTSLIGDPSGRNATRPPLTREQIEANAKTYFEQAALVLDRDKTEIRYN
HCCCEEEEEHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCEEEEC
SEWSMPLGADGMIKLASRYTVARILEREDFTKRFQGGVPISIHEFLYPLMQGYDSVALNA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCEEEEC
DLELGGTDQKFNLLVGRELQKQYGQEQQCILTMPLLEGLDGVEKMSKSKGNYVGISEKPT
CEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHCCHHHHHHHHCCCCEECCCCCCH
DMFGKLMSISDTLMWRYFELLSFRSMDEIAGFKREAEGGRNPRDFKVMLAQEIVARFHSQ
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
ADAERALEDFNHRAKGGVPDDIPSVSLAGAPLAIGQLLKQAGLVPSTSEALRNIEQGGVK
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCEE
IDGTTVSDKGLKVDAGEYVVQVGKRRFARVTLAA
ECCEEECCCCCEECHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA