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The map label for this gene is tgt [H]

Identifier: 161525833

GI number: 161525833

Start: 2909583

End: 2910782

Strand: Direct

Name: tgt [H]

Synonym: Bmul_2664

Alternate gene names: 161525833

Gene position: 2909583-2910782 (Clockwise)

Preceding gene: 161525832

Following gene: 161525834

Centisome position: 84.37

GC content: 66.08

Gene sequence:

>1200_bases
ATGACCGAAGGTTCATCCCACGATACGCGCGCGCACGCGCCCGAAGCCGCACCCGCCCGCCCCGCCAACGGGCTCGAATT
CGAACTGCTGACGACCGACGGCCACGCGCGCCGCGGCCGCGTGAAGCTCAACCACGGCGTCGTCGAGACGCCGATCTTCA
TGCCGGTCGGCACCTACGGCACCGTGAAGGCGATCCAGCCGCGCGAGCTGCACGAGATCAAGGCGCAGATCATCCTCGGC
AACACGTTCCACCTGTGGCTGCGCCCGGGCCTCGACACGATCGACGCACACGGCGGCCTGCACGGCTTCATGGGCTGGAA
CAAGCCGATCCTGACGGACTCGGGCGGCTTCCAGGTGTTCTCGCTCGGCGACCTGCGCAAGATCACCGAGGACGGCGTCA
CGTTCGCGTCGCCGATCAACGGCGACAAGCTGTTCCTGTCGCCGGAAGTGTCGATGCAGATCCAGAAGGTGCTGAACTCG
GACATCGTGATGCAGTTCGACGAATGCACGCCGTACGCGACCAACGGCGTGCCGACCACGCACAAGGAAGCGGCCGACTC
GATGCGCATGTCGCTGCGCTGGGCAAAGCGCTCGCTCGACGAGTTCAACCGGCTCGGCAACCCGAACGCGCTGTTCGGGA
TCGTCCAGGGCGGGATGTTCGAGGATCTGCGCGACGAATCGCTCGCGGGCCTGTCGGAACTCGGCTTCCACGGCCTCGCG
ATCGGCGGGCTGTCGGTCGGCGAGCCGAAGGAGGACATGATGCGCGTGCTGCGCCACGTCGGCCCGAAGCTGCCGGCGCA
CAAGCCGCACTACCTGATGGGCGTCGGCACGCCCGAGGATCTCGTCGAGGGCGTCGCGAATGGCGTCGACATGTTCGACT
GCGTGATGCCTACGCGCAACGCGCGCAACGGCTGGCTCTTCACGCGCTTCGGCGACGTGAAGATCCGCAACGCGACGCAC
AAGAATTCGCTGAAGCCGCTCGATCCGAGCTGCACGTGCTACACGTGCAGCAACTTCTCGCGCGGCTATCTGCACCACCT
GCATCGCGTGGGCGAAATCCTCGGCGCGCAGCTGAACACGATCCACAACCTGCACTACTACCTGCAGCTGATGAGCGAGA
TCCGCGACGCGATCGAATCGCACACGTTCGACGCGTTCCGCCAGCGCTTCGCGGAGGATCGCGCGAGAGGCGTCGACTGA

Upstream 100 bases:

>100_bases
GCGCTACGATGGCGCACATCCATTCTTTGCGCCGCCGCTACCGCGGTGGCTTTTTCCAACTCGCGCCGGACTGTTTTCCG
GTGGCCAGGAGCACCCCACG

Downstream 100 bases:

>100_bases
CGGGCCTGGCCGGGCGTGGCCGGCGCATCGTATGCGAACGAAATAATGCGTCGCGCCGCTCGGTCCCGATCGCCAAAACA
TTACAATTACCTTTCCAAAC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTEGSSHDTRAHAPEAAPARPANGLEFELLTTDGHARRGRVKLNHGVVETPIFMPVGTYGTVKAIQPRELHEIKAQIILG
NTFHLWLRPGLDTIDAHGGLHGFMGWNKPILTDSGGFQVFSLGDLRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNS
DIVMQFDECTPYATNGVPTTHKEAADSMRMSLRWAKRSLDEFNRLGNPNALFGIVQGGMFEDLRDESLAGLSELGFHGLA
IGGLSVGEPKEDMMRVLRHVGPKLPAHKPHYLMGVGTPEDLVEGVANGVDMFDCVMPTRNARNGWLFTRFGDVKIRNATH
KNSLKPLDPSCTCYTCSNFSRGYLHHLHRVGEILGAQLNTIHNLHYYLQLMSEIRDAIESHTFDAFRQRFAEDRARGVD

Sequences:

>Translated_399_residues
MTEGSSHDTRAHAPEAAPARPANGLEFELLTTDGHARRGRVKLNHGVVETPIFMPVGTYGTVKAIQPRELHEIKAQIILG
NTFHLWLRPGLDTIDAHGGLHGFMGWNKPILTDSGGFQVFSLGDLRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNS
DIVMQFDECTPYATNGVPTTHKEAADSMRMSLRWAKRSLDEFNRLGNPNALFGIVQGGMFEDLRDESLAGLSELGFHGLA
IGGLSVGEPKEDMMRVLRHVGPKLPAHKPHYLMGVGTPEDLVEGVANGVDMFDCVMPTRNARNGWLFTRFGDVKIRNATH
KNSLKPLDPSCTCYTCSNFSRGYLHHLHRVGEILGAQLNTIHNLHYYLQLMSEIRDAIESHTFDAFRQRFAEDRARGVD
>Mature_398_residues
TEGSSHDTRAHAPEAAPARPANGLEFELLTTDGHARRGRVKLNHGVVETPIFMPVGTYGTVKAIQPRELHEIKAQIILGN
TFHLWLRPGLDTIDAHGGLHGFMGWNKPILTDSGGFQVFSLGDLRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSD
IVMQFDECTPYATNGVPTTHKEAADSMRMSLRWAKRSLDEFNRLGNPNALFGIVQGGMFEDLRDESLAGLSELGFHGLAI
GGLSVGEPKEDMMRVLRHVGPKLPAHKPHYLMGVGTPEDLVEGVANGVDMFDCVMPTRNARNGWLFTRFGDVKIRNATHK
NSLKPLDPSCTCYTCSNFSRGYLHHLHRVGEILGAQLNTIHNLHYYLQLMSEIRDAIESHTFDAFRQRFAEDRARGVD

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=355, Percent_Identity=44.5070422535211, Blast_Score=296, Evalue=2e-80,
Organism=Escherichia coli, GI1786607, Length=374, Percent_Identity=64.7058823529412, Blast_Score=496, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI17544680, Length=371, Percent_Identity=42.5876010781671, Blast_Score=294, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI25146502, Length=139, Percent_Identity=28.0575539568345, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19920510, Length=355, Percent_Identity=44.2253521126761, Blast_Score=304, Evalue=7e-83,
Organism=Drosophila melanogaster, GI21355311, Length=158, Percent_Identity=27.8481012658228, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 44247; Mature: 44116

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEGSSHDTRAHAPEAAPARPANGLEFELLTTDGHARRGRVKLNHGVVETPIFMPVGTYG
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCEEECCEEEECCCCC
TVKAIQPRELHEIKAQIILGNTFHLWLRPGLDTIDAHGGLHGFMGWNKPILTDSGGFQVF
CEECCCCHHHHHHEEEEEECCEEEEEEECCCCCCCCCCCCEECCCCCCCEEECCCCEEEE
SLGDLRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDIVMQFDECTPYATNGVPTT
ECCHHHHHHHCCCEEECCCCCCEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCC
HKEAADSMRMSLRWAKRSLDEFNRLGNPNALFGIVQGGMFEDLRDESLAGLSELGFHGLA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEE
IGGLSVGEPKEDMMRVLRHVGPKLPAHKPHYLMGVGTPEDLVEGVANGVDMFDCVMPTRN
ECCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCC
ARNGWLFTRFGDVKIRNATHKNSLKPLDPSCTCYTCSNFSRGYLHHLHRVGEILGAQLNT
CCCCEEEEEECCEEEECCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
IHNLHYYLQLMSEIRDAIESHTFDAFRQRFAEDRARGVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TEGSSHDTRAHAPEAAPARPANGLEFELLTTDGHARRGRVKLNHGVVETPIFMPVGTYG
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCEEECCEEEECCCCC
TVKAIQPRELHEIKAQIILGNTFHLWLRPGLDTIDAHGGLHGFMGWNKPILTDSGGFQVF
CEECCCCHHHHHHEEEEEECCEEEEEEECCCCCCCCCCCCEECCCCCCCEEECCCCEEEE
SLGDLRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDIVMQFDECTPYATNGVPTT
ECCHHHHHHHCCCEEECCCCCCEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCC
HKEAADSMRMSLRWAKRSLDEFNRLGNPNALFGIVQGGMFEDLRDESLAGLSELGFHGLA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEE
IGGLSVGEPKEDMMRVLRHVGPKLPAHKPHYLMGVGTPEDLVEGVANGVDMFDCVMPTRN
ECCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCC
ARNGWLFTRFGDVKIRNATHKNSLKPLDPSCTCYTCSNFSRGYLHHLHRVGEILGAQLNT
CCCCEEEEEECCEEEECCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
IHNLHYYLQLMSEIRDAIESHTFDAFRQRFAEDRARGVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA