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The map label for this gene is queA [H]

Identifier: 161525832

GI number: 161525832

Start: 2908296

End: 2909402

Strand: Direct

Name: queA [H]

Synonym: Bmul_2663

Alternate gene names: 161525832

Gene position: 2908296-2909402 (Clockwise)

Preceding gene: 161525825

Following gene: 161525833

Centisome position: 84.34

GC content: 69.38

Gene sequence:

>1107_bases
ATGCCGGCGGCGCCCGCCGGCCGTTTCGAACCAGCCCGCATGTTCACGCTTTCCGATTTCGATTTCAATCTGCCGCCCGA
GCTGATTGCGCAAACCGCGCTGCCCGACCGCACCGCGAGCCGCCTGCTCGAGGTCGACGCGACGGCCGAGCCCGCGCGCT
TCGTCGATCGCCGTTTCGCCGAATTGCCGGCCTGCATCGCGCGCGGCGACCTGCTCGTGTTCAACGATACCAAGGTGCTG
AAGGCGCGCTTCTTCGGCCAGAAGGCGAGCGGCGGCCGGATCGAGGTGCTGATCGAGCGCGTGACGGGCTCGCATACGGC
GCTCGCGCAGATCCGCGCGAGCAAGTCGCCGGGCGCCGGCACGACGCTGCGCCTCGCCGACGCGTTCGACGTGACGGTCG
GCGAACGCGTCGAGCCGTTCTTCACGCTGCACTTTCCGCAGCCGTGCCTCGAGCTGATCGAGCAGTACGGCCGCCTGCCG
CTGCCGCCGTACATCGAGCACGATCCGGACGCGACCGACGAGACGCGCTATCAGACCGTCTATGCGAGCAACCCGGGCGC
GGTCGCCGCGCCGACGGCCGGCCTGCACTTCGACGAGCCGCTGCTCGCGAAGCTCGACGCGATGGGCATCGAGCGCGCGA
CGCTGACGCTGCACGTCGGCGCGGGCACGTTCCAGCCGGTGCGCGTCGAGAACATCGCCGAGCACAAGATGCACAGCGAG
TGGTACGAACTGCCGCAGTCGCTCGTCGACAAGATCGCGGCCACACGCGCGCGCGGCGGCAAGGTGATCGCGGTCGGCAC
GACGTCGATGCGTGCGCTCGAAGCCGCGGCGCGCGATGCCGAAGCCGCCGGTCGGCCGCTCGCCGCGACGCAGGCCGAGA
CCGACATCTTCATCACGCCCGGCTACCGTTTCCGCGTGGTCGACCGGCTCGTCACGAATTTCCATCTGCCGAAGTCGACG
CTGATGATGCTGGTGTCCGCGTTCGCGGGCATCGAGACGATCCGCGCCGCGTACCGCCACGCGATCGAACAGCGCTACCG
CTTCTTCAGCTACGGCGACGCGATGCTGCTCACACGGCGCGATACGCCGGAGGCGACGCACGCGTAA

Upstream 100 bases:

>100_bases
GTACAATAGCGGCTTTCACGTCGTCCGAACGAACGACGCCGGCCAGGCCGCGCATCCCGCCATCATAGCGGCTCGCGCGC
GCCCTTTACGCGTTTCCGCC

Downstream 100 bases:

>100_bases
GGCGTCGTCCGTGCGTCGGCCGGCGTACGGCACGGCCGCGGCGGCGCCTTCGGCACGGGACCGCCGCTCGCCTGCGCCGC
GCGCTACGATGGCGCACATC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MPAAPAGRFEPARMFTLSDFDFNLPPELIAQTALPDRTASRLLEVDATAEPARFVDRRFAELPACIARGDLLVFNDTKVL
KARFFGQKASGGRIEVLIERVTGSHTALAQIRASKSPGAGTTLRLADAFDVTVGERVEPFFTLHFPQPCLELIEQYGRLP
LPPYIEHDPDATDETRYQTVYASNPGAVAAPTAGLHFDEPLLAKLDAMGIERATLTLHVGAGTFQPVRVENIAEHKMHSE
WYELPQSLVDKIAATRARGGKVIAVGTTSMRALEAAARDAEAAGRPLAATQAETDIFITPGYRFRVVDRLVTNFHLPKST
LMMLVSAFAGIETIRAAYRHAIEQRYRFFSYGDAMLLTRRDTPEATHA

Sequences:

>Translated_368_residues
MPAAPAGRFEPARMFTLSDFDFNLPPELIAQTALPDRTASRLLEVDATAEPARFVDRRFAELPACIARGDLLVFNDTKVL
KARFFGQKASGGRIEVLIERVTGSHTALAQIRASKSPGAGTTLRLADAFDVTVGERVEPFFTLHFPQPCLELIEQYGRLP
LPPYIEHDPDATDETRYQTVYASNPGAVAAPTAGLHFDEPLLAKLDAMGIERATLTLHVGAGTFQPVRVENIAEHKMHSE
WYELPQSLVDKIAATRARGGKVIAVGTTSMRALEAAARDAEAAGRPLAATQAETDIFITPGYRFRVVDRLVTNFHLPKST
LMMLVSAFAGIETIRAAYRHAIEQRYRFFSYGDAMLLTRRDTPEATHA
>Mature_367_residues
PAAPAGRFEPARMFTLSDFDFNLPPELIAQTALPDRTASRLLEVDATAEPARFVDRRFAELPACIARGDLLVFNDTKVLK
ARFFGQKASGGRIEVLIERVTGSHTALAQIRASKSPGAGTTLRLADAFDVTVGERVEPFFTLHFPQPCLELIEQYGRLPL
PPYIEHDPDATDETRYQTVYASNPGAVAAPTAGLHFDEPLLAKLDAMGIERATLTLHVGAGTFQPVRVENIAEHKMHSEW
YELPQSLVDKIAATRARGGKVIAVGTTSMRALEAAARDAEAAGRPLAATQAETDIFITPGYRFRVVDRLVTNFHLPKSTL
MMLVSAFAGIETIRAAYRHAIEQRYRFFSYGDAMLLTRRDTPEATHA

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=346, Percent_Identity=52.0231213872832, Blast_Score=343, Evalue=7e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 40464; Mature: 40332

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAAPAGRFEPARMFTLSDFDFNLPPELIAQTALPDRTASRLLEVDATAEPARFVDRRFA
CCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCHHHHHHEECCCCCCHHHHHHHHHH
ELPACIARGDLLVFNDTKVLKARFFGQKASGGRIEVLIERVTGSHTALAQIRASKSPGAG
HHHHHHHCCCEEEECCCHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCC
TTLRLADAFDVTVGERVEPFFTLHFPQPCLELIEQYGRLPLPPYIEHDPDATDETRYQTV
CEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEE
YASNPGAVAAPTAGLHFDEPLLAKLDAMGIERATLTLHVGAGTFQPVRVENIAEHKMHSE
EECCCCCEECCCCCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCCEEHHHHHHHHHHHH
WYELPQSLVDKIAATRARGGKVIAVGTTSMRALEAAARDAEAAGRPLAATQAETDIFITP
HHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCEECCCCCEEEECC
GYRFRVVDRLVTNFHLPKSTLMMLVSAFAGIETIRAAYRHAIEQRYRFFSYGDAMLLTRR
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
DTPEATHA
CCCCCCCC
>Mature Secondary Structure 
PAAPAGRFEPARMFTLSDFDFNLPPELIAQTALPDRTASRLLEVDATAEPARFVDRRFA
CCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCHHHHHHEECCCCCCHHHHHHHHHH
ELPACIARGDLLVFNDTKVLKARFFGQKASGGRIEVLIERVTGSHTALAQIRASKSPGAG
HHHHHHHCCCEEEECCCHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCC
TTLRLADAFDVTVGERVEPFFTLHFPQPCLELIEQYGRLPLPPYIEHDPDATDETRYQTV
CEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEE
YASNPGAVAAPTAGLHFDEPLLAKLDAMGIERATLTLHVGAGTFQPVRVENIAEHKMHSE
EECCCCCEECCCCCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCCEEHHHHHHHHHHHH
WYELPQSLVDKIAATRARGGKVIAVGTTSMRALEAAARDAEAAGRPLAATQAETDIFITP
HHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCEECCCCCEEEECC
GYRFRVVDRLVTNFHLPKSTLMMLVSAFAGIETIRAAYRHAIEQRYRFFSYGDAMLLTRR
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
DTPEATHA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA