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The map label for this gene is epsC [H]

Identifier: 161525673

GI number: 161525673

Start: 2720650

End: 2722536

Strand: Reverse

Name: epsC [H]

Synonym: Bmul_2504

Alternate gene names: 161525673

Gene position: 2722536-2720650 (Counterclockwise)

Preceding gene: 161525674

Following gene: 161525666

Centisome position: 78.95

GC content: 63.7

Gene sequence:

>1887_bases
ATGTTGCGATCCAAAGCATCATGGCTGTCGCTGAGTGCTTTCTTTTTCGACCTGACGGCGGTTGTCGCCGCGTGGCTGTG
CGCTTATCTGATCCGCTTCAACGGCAGCATCCCGCCTGATTTCCTGCGCGGTGCGCTGACCGCGATGGGATGGGCACTGC
CCGTCTACGCCCTGATGTTCCACATCTTCGGGCTCTATCGAGGCCTATGGGTGTTCGCGAGCCTACCGGATCTGATGCGC
ATCTCGAAGGCGGTCGGCGGCGGCGCCGTGATCGTGATGATCGGCGCCGTGATGTTCCAGCCGATGCCCATCATCCCGCG
CTCGGTGCTGCTTGTCTCGCCGCTGATGCTGTTCCTCGCAATGGGCGGCGCCCGCGCGCTCTACCGCGCGACGAAGGAGT
TCTACCTGTACGGCGGGCTCGTTGGCCAGGGGAAGCCCGTGCTCGTGCTCGGCGCCGGCACGGCCGGCGCGAATCTCGCG
CGCGAGCTGTCCCGTTCCGGCGAATGGCGGCTCGTCGGCCTGCTCGACGACGACGTCACGAAGCAGGGCCGTGAAATCTA
CGGATACAAAGTGCTGGGCTCGTTCAACGACCTGAAGCTCTGGACCGACGCGACGAAGGTCGAATACGCGATCATCGCGA
TTCCGTCGGCGTCCGTCGAAACGCAGCGACGTGTCGCGACGCTGTGCGTGCGCGCCGGCGTGAAGGCGATGGTACTGCCG
TCGTTGACCGCGTTGATGCCGGGGCAGGGCTTCCTGTCCCAGGTGCGCAATATCGATCTCGAAGACCTGCTCGGTCGAGA
GGCGGTAACGATCGACACGCCGCACGTCGAAGCGCTCCTGCGCGGCCGTGTCGTGATGGTCACGGGCGCCGGGGGGTCGA
TCGGCTCCGAGCTGTGCCGGCAGATCCTCCGCTTCGCGCCGGCGCAGCTCGTCGCGTTTGATTTGTCCGAATACGCAATG
TATCGGCTCGTCGAGGAACTGCGCGAACGCTTCCCTGATCAGCCCGTCGTGCCGATTATCGGTGACGCGAAGGATTCGCT
GCTGCTCGATCAGGTGATGTCGCGCTACGCACCGCACATCGTCTTCCATGCGGCCGCGTACAAGCACGTGCCGCTGATGG
AGGAGCACAACGCGTGGCAGGCGCTGCGCAACAACGTGCTCGGCACGTATCGCGTGGCGCGCGCGGCGATTCGCCACGAT
GTCCGTCACTTCGTGCTGATCTCGACCGACAAGGCCGTCAATCCGACCAACGTGATGGGCGCCAGCAAGCGGCTCGCCGA
AATGGCATGTCAGGCGCTGCAGCAGACGAGCGACCGCACGCAGTTCGAGACCGTGCGTTTTGGCAACGTGCTGGGCAGCG
CGGGCAGCGTGATTCCGAAGTTCCAGCAGCAAATCGCGAAGGGTGGGCCGGTCACAGTCACGCATCCCGAGATCACCCGA
TTTTTCATGACGATCCCCGAGGCGTCGCAGCTCGTGCTGCAGGCGTCGAGCATGGGCCGCGGCGGTGAGATCTTTATTCT
CGACATGGGCCAGCCGGTGAAGATCGTCGATCTAGCGCGCGACCTGATCCGCCTGTACGGCTTTTCCGAGGAGCAGATCC
GCATCGAATTCACTGGCCTGCGTCCGGGTGAAAAACTCTACGAGGAGTTGCTCGCGGACGACGAAACGACGACGCGCACG
CCGCATCCGAAGCTGCGCATTGCTCGGGCACGAGAAGTGCCGGACCATCTGCTTGATGAACTATTGCCGTGGCTGATGCA
GCACCGCGTGCTGACCGACGACGAGGTCCGGCGCGATCTGCGACGCTGGGTGCCGGAATATCAGACAGCATCGGTACCTA
TGTTGCAAAGCGTCCCGACGGCAGTACGTGCGGTATCAGAGGGTTAA

Upstream 100 bases:

>100_bases
CGCATGGTACGGCGTCCTGGCATGTATCGGAGTGGTGATCGACATGCGTTGGCGCCGGCTTCAGACGGCCGCCAAAAACA
ATTCGTGAGGTTTCCCGCCG

Downstream 100 bases:

>100_bases
TGGTGGTTCGGATACGCGGGGCTAGTCGCGAGCCCGAACTGCAGTTGGGACCTGCAAGACCGTTCGCGGATTAGCCGAGG
CATGTCGAGAGATGTCATGC

Product: polysaccharide biosynthesis protein CapD

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 628; Mature: 628

Protein sequence:

>628_residues
MLRSKASWLSLSAFFFDLTAVVAAWLCAYLIRFNGSIPPDFLRGALTAMGWALPVYALMFHIFGLYRGLWVFASLPDLMR
ISKAVGGGAVIVMIGAVMFQPMPIIPRSVLLVSPLMLFLAMGGARALYRATKEFYLYGGLVGQGKPVLVLGAGTAGANLA
RELSRSGEWRLVGLLDDDVTKQGREIYGYKVLGSFNDLKLWTDATKVEYAIIAIPSASVETQRRVATLCVRAGVKAMVLP
SLTALMPGQGFLSQVRNIDLEDLLGREAVTIDTPHVEALLRGRVVMVTGAGGSIGSELCRQILRFAPAQLVAFDLSEYAM
YRLVEELRERFPDQPVVPIIGDAKDSLLLDQVMSRYAPHIVFHAAAYKHVPLMEEHNAWQALRNNVLGTYRVARAAIRHD
VRHFVLISTDKAVNPTNVMGASKRLAEMACQALQQTSDRTQFETVRFGNVLGSAGSVIPKFQQQIAKGGPVTVTHPEITR
FFMTIPEASQLVLQASSMGRGGEIFILDMGQPVKIVDLARDLIRLYGFSEEQIRIEFTGLRPGEKLYEELLADDETTTRT
PHPKLRIARAREVPDHLLDELLPWLMQHRVLTDDEVRRDLRRWVPEYQTASVPMLQSVPTAVRAVSEG

Sequences:

>Translated_628_residues
MLRSKASWLSLSAFFFDLTAVVAAWLCAYLIRFNGSIPPDFLRGALTAMGWALPVYALMFHIFGLYRGLWVFASLPDLMR
ISKAVGGGAVIVMIGAVMFQPMPIIPRSVLLVSPLMLFLAMGGARALYRATKEFYLYGGLVGQGKPVLVLGAGTAGANLA
RELSRSGEWRLVGLLDDDVTKQGREIYGYKVLGSFNDLKLWTDATKVEYAIIAIPSASVETQRRVATLCVRAGVKAMVLP
SLTALMPGQGFLSQVRNIDLEDLLGREAVTIDTPHVEALLRGRVVMVTGAGGSIGSELCRQILRFAPAQLVAFDLSEYAM
YRLVEELRERFPDQPVVPIIGDAKDSLLLDQVMSRYAPHIVFHAAAYKHVPLMEEHNAWQALRNNVLGTYRVARAAIRHD
VRHFVLISTDKAVNPTNVMGASKRLAEMACQALQQTSDRTQFETVRFGNVLGSAGSVIPKFQQQIAKGGPVTVTHPEITR
FFMTIPEASQLVLQASSMGRGGEIFILDMGQPVKIVDLARDLIRLYGFSEEQIRIEFTGLRPGEKLYEELLADDETTTRT
PHPKLRIARAREVPDHLLDELLPWLMQHRVLTDDEVRRDLRRWVPEYQTASVPMLQSVPTAVRAVSEG
>Mature_628_residues
MLRSKASWLSLSAFFFDLTAVVAAWLCAYLIRFNGSIPPDFLRGALTAMGWALPVYALMFHIFGLYRGLWVFASLPDLMR
ISKAVGGGAVIVMIGAVMFQPMPIIPRSVLLVSPLMLFLAMGGARALYRATKEFYLYGGLVGQGKPVLVLGAGTAGANLA
RELSRSGEWRLVGLLDDDVTKQGREIYGYKVLGSFNDLKLWTDATKVEYAIIAIPSASVETQRRVATLCVRAGVKAMVLP
SLTALMPGQGFLSQVRNIDLEDLLGREAVTIDTPHVEALLRGRVVMVTGAGGSIGSELCRQILRFAPAQLVAFDLSEYAM
YRLVEELRERFPDQPVVPIIGDAKDSLLLDQVMSRYAPHIVFHAAAYKHVPLMEEHNAWQALRNNVLGTYRVARAAIRHD
VRHFVLISTDKAVNPTNVMGASKRLAEMACQALQQTSDRTQFETVRFGNVLGSAGSVIPKFQQQIAKGGPVTVTHPEITR
FFMTIPEASQLVLQASSMGRGGEIFILDMGQPVKIVDLARDLIRLYGFSEEQIRIEFTGLRPGEKLYEELLADDETTTRT
PHPKLRIARAREVPDHLLDELLPWLMQHRVLTDDEVRRDLRRWVPEYQTASVPMLQSVPTAVRAVSEG

Specific function: Involved in biofilm formation [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 69557; Mature: 69557

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRSKASWLSLSAFFFDLTAVVAAWLCAYLIRFNGSIPPDFLRGALTAMGWALPVYALMF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
HIFGLYRGLWVFASLPDLMRISKAVGGGAVIVMIGAVMFQPMPIIPRSVLLVSPLMLFLA
HHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
MGGARALYRATKEFYLYGGLVGQGKPVLVLGAGTAGANLARELSRSGEWRLVGLLDDDVT
CCCHHHHHHHHHHHEEECCEECCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEECCCHHH
KQGREIYGYKVLGSFNDLKLWTDATKVEYAIIAIPSASVETQRRVATLCVRAGVKAMVLP
HCCCCEEEEEEECCCCCCEEEECCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHH
SLTALMPGQGFLSQVRNIDLEDLLGREAVTIDTPHVEALLRGRVVMVTGAGGSIGSELCR
HHHHHCCCHHHHHHHHCCCHHHHCCCCEEEECCHHHHHHHCCCEEEEECCCCCHHHHHHH
QILRFAPAQLVAFDLSEYAMYRLVEELRERFPDQPVVPIIGDAKDSLLLDQVMSRYAPHI
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCE
VFHAAAYKHVPLMEEHNAWQALRNNVLGTYRVARAAIRHDVRHFVLISTDKAVNPTNVMG
EEEHHHHHCCCCHHHCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
ASKRLAEMACQALQQTSDRTQFETVRFGNVLGSAGSVIPKFQQQIAKGGPVTVTHPEITR
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEECHHHHH
FFMTIPEASQLVLQASSMGRGGEIFILDMGQPVKIVDLARDLIRLYGFSEEQIRIEFTGL
HHHCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECC
RPGEKLYEELLADDETTTRTPHPKLRIARAREVPDHLLDELLPWLMQHRVLTDDEVRRDL
CCHHHHHHHHHCCCCCCCCCCCCCEEHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
RRWVPEYQTASVPMLQSVPTAVRAVSEG
HHHCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLRSKASWLSLSAFFFDLTAVVAAWLCAYLIRFNGSIPPDFLRGALTAMGWALPVYALMF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
HIFGLYRGLWVFASLPDLMRISKAVGGGAVIVMIGAVMFQPMPIIPRSVLLVSPLMLFLA
HHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
MGGARALYRATKEFYLYGGLVGQGKPVLVLGAGTAGANLARELSRSGEWRLVGLLDDDVT
CCCHHHHHHHHHHHEEECCEECCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEECCCHHH
KQGREIYGYKVLGSFNDLKLWTDATKVEYAIIAIPSASVETQRRVATLCVRAGVKAMVLP
HCCCCEEEEEEECCCCCCEEEECCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHH
SLTALMPGQGFLSQVRNIDLEDLLGREAVTIDTPHVEALLRGRVVMVTGAGGSIGSELCR
HHHHHCCCHHHHHHHHCCCHHHHCCCCEEEECCHHHHHHHCCCEEEEECCCCCHHHHHHH
QILRFAPAQLVAFDLSEYAMYRLVEELRERFPDQPVVPIIGDAKDSLLLDQVMSRYAPHI
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCE
VFHAAAYKHVPLMEEHNAWQALRNNVLGTYRVARAAIRHDVRHFVLISTDKAVNPTNVMG
EEEHHHHHCCCCHHHCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
ASKRLAEMACQALQQTSDRTQFETVRFGNVLGSAGSVIPKFQQQIAKGGPVTVTHPEITR
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEECHHHHH
FFMTIPEASQLVLQASSMGRGGEIFILDMGQPVKIVDLARDLIRLYGFSEEQIRIEFTGL
HHHCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECC
RPGEKLYEELLADDETTTRTPHPKLRIARAREVPDHLLDELLPWLMQHRVLTDDEVRRDL
CCHHHHHHHHHCCCCCCCCCCCCCEEHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
RRWVPEYQTASVPMLQSVPTAVRAVSEG
HHHCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]