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The map label for this gene is trmB [H]

Identifier: 161525642

GI number: 161525642

Start: 2688875

End: 2689705

Strand: Direct

Name: trmB [H]

Synonym: Bmul_2473

Alternate gene names: 161525642

Gene position: 2688875-2689705 (Clockwise)

Preceding gene: 161525641

Following gene: 161525646

Centisome position: 77.97

GC content: 68.35

Gene sequence:

>831_bases
ATGTGCCGGTTCCGCCGGTCACCGCGCCCGAGCCCTCGCTCGCAACCGAGCCAATCTCTGTCACCGATGATGCACGACGA
TCCGAACGAAGCCGGCCTGCCGCCGCACGACGACGCCCTGTCCGACGAAGCCGCCGACGGCACCGAAGTCAATCCGCTGC
ACCGCCGCCGCATCCGCAGCTTCGTCACGCGCGCCGGGCGCGTATCCACCGGCCAGCGCCGCGCGCTCGACGAACTGGGC
CCGCGCTTCGTCGTCCCGTACGCACCCGAAACGCCCGACTGGAATGCGATCTTCGGCCGCAGCGCACCGCGCATTCTCGA
AATCGGCTTCGGGATGGGCGCGTCGACGGCCGAAATCGCCGCGAATCGGCCGGGCGACGACTTCCTCGGCGTCGAAGTGC
ACGAACCGGGCGTCGGCGCGCTGCTGAAGCTGATCGGCGAACAGGATCTGTCGAACATCCGCATCATCCAGCACGATGCG
GTCGAAGTGCTCGAACACATGCTCACGCCCGAAAGCCTCGACGGCGTGCACATCTTCTTCCCCGATCCGTGGCACAAGGC
GCGGCACCACAAGCGCCGCCTGATTCAGCCGCCGCTCGTCGCGCAACTCGCATCGCGCCTGAAACCGGGCGCGTACCTGC
ATTGCGCGACCGACTGGCAGAACTACGCGGAGCAGATGCTCGAGGTGCTGAGCGCCGAGCCGACGCTCGAAAACACGGCC
GACGGCTACGCGCCGCGCCCCGACTATCGGCCCGTCACGAAGTTCGAACGACGCGGGCTGCGGCTCGGTCACGGCGTCTG
GGATCTCGTGTTCCGCAAGCGCGCGGGCTGA

Upstream 100 bases:

>100_bases
GTGTTCGAAGCGGGGGTTCCATTCCCATCGCCCGCTCCACTTTCCGGCTGCACTGCAGCCAGCGCGCAAGCCGCCTCGTC
GAAGGCGGCTTTTTTGTACC

Downstream 100 bases:

>100_bases
TTCACCGGCAGCAGCGCGCTGCCGCCCTCGCCAAAGACAACGGCCCGACCGGCATCTGCCGATCGGGCCGTTTTCACATC
CGCGCCCTACTCCCCACGAA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MCRFRRSPRPSPRSQPSQSLSPMMHDDPNEAGLPPHDDALSDEAADGTEVNPLHRRRIRSFVTRAGRVSTGQRRALDELG
PRFVVPYAPETPDWNAIFGRSAPRILEIGFGMGASTAEIAANRPGDDFLGVEVHEPGVGALLKLIGEQDLSNIRIIQHDA
VEVLEHMLTPESLDGVHIFFPDPWHKARHHKRRLIQPPLVAQLASRLKPGAYLHCATDWQNYAEQMLEVLSAEPTLENTA
DGYAPRPDYRPVTKFERRGLRLGHGVWDLVFRKRAG

Sequences:

>Translated_276_residues
MCRFRRSPRPSPRSQPSQSLSPMMHDDPNEAGLPPHDDALSDEAADGTEVNPLHRRRIRSFVTRAGRVSTGQRRALDELG
PRFVVPYAPETPDWNAIFGRSAPRILEIGFGMGASTAEIAANRPGDDFLGVEVHEPGVGALLKLIGEQDLSNIRIIQHDA
VEVLEHMLTPESLDGVHIFFPDPWHKARHHKRRLIQPPLVAQLASRLKPGAYLHCATDWQNYAEQMLEVLSAEPTLENTA
DGYAPRPDYRPVTKFERRGLRLGHGVWDLVFRKRAG
>Mature_276_residues
MCRFRRSPRPSPRSQPSQSLSPMMHDDPNEAGLPPHDDALSDEAADGTEVNPLHRRRIRSFVTRAGRVSTGQRRALDELG
PRFVVPYAPETPDWNAIFGRSAPRILEIGFGMGASTAEIAANRPGDDFLGVEVHEPGVGALLKLIGEQDLSNIRIIQHDA
VEVLEHMLTPESLDGVHIFFPDPWHKARHHKRRLIQPPLVAQLASRLKPGAYLHCATDWQNYAEQMLEVLSAEPTLENTA
DGYAPRPDYRPVTKFERRGLRLGHGVWDLVFRKRAG

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=220, Percent_Identity=55, Blast_Score=244, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6320000, Length=170, Percent_Identity=26.4705882352941, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 30926; Mature: 30926

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRFRRSPRPSPRSQPSQSLSPMMHDDPNEAGLPPHDDALSDEAADGTEVNPLHRRRIRS
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
FVTRAGRVSTGQRRALDELGPRFVVPYAPETPDWNAIFGRSAPRILEIGFGMGASTAEIA
HHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCCCCEECCCCCCCEEEECCCCCCCHHHHH
ANRPGDDFLGVEVHEPGVGALLKLIGEQDLSNIRIIQHDAVEVLEHMLTPESLDGVHIFF
CCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCCCEEEEC
PDPWHKARHHKRRLIQPPLVAQLASRLKPGAYLHCATDWQNYAEQMLEVLSAEPTLENTA
CCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCC
DGYAPRPDYRPVTKFERRGLRLGHGVWDLVFRKRAG
CCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MCRFRRSPRPSPRSQPSQSLSPMMHDDPNEAGLPPHDDALSDEAADGTEVNPLHRRRIRS
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
FVTRAGRVSTGQRRALDELGPRFVVPYAPETPDWNAIFGRSAPRILEIGFGMGASTAEIA
HHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCCCCEECCCCCCCEEEECCCCCCCHHHHH
ANRPGDDFLGVEVHEPGVGALLKLIGEQDLSNIRIIQHDAVEVLEHMLTPESLDGVHIFF
CCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCCCEEEEC
PDPWHKARHHKRRLIQPPLVAQLASRLKPGAYLHCATDWQNYAEQMLEVLSAEPTLENTA
CCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCC
DGYAPRPDYRPVTKFERRGLRLGHGVWDLVFRKRAG
CCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA