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The map label for this gene is bcr [H]

Identifier: 161525604

GI number: 161525604

Start: 2649518

End: 2650735

Strand: Reverse

Name: bcr [H]

Synonym: Bmul_2434

Alternate gene names: 161525604

Gene position: 2650735-2649518 (Counterclockwise)

Preceding gene: 161525614

Following gene: 161525600

Centisome position: 76.87

GC content: 69.87

Gene sequence:

>1218_bases
ATGTCTCACGTCGTCCGGCGCCGGCCCGATGCCCGGCTGATCCTGTTGCTCGGCGCGCTGGCCGCGTGCGGGCCGATCGC
TACCGACATGTACCTGCCGAGCCTGCCGTCGATCGCGCAAGGCTTCTCCGTCAACCCGGGCGCCGCGCAGCGCACGCTGA
CGAGCTTCATGGCCGGCTTCTCGATCGGCATGCTGCTGTACGGCCCGCTGTCCGACACCTGGGGCCGCAGGCCCGTGCTG
CTCGGCGGGATCGCGCTGTTTACGCTCGCGAGCGTCGGCTGCTTCGTGTCGACGTCGATCGACATGCTGATCGTCGTGCG
CTTTCTGCAGGCGCTCGGCGCCGGTGCGGCGTCGGTGCTCGCGCGGGCGATCGCGCGCGATGCGCACGAGCCGACCGATG
CGGCGAAAGTGCTGTCGATGGTCGCGATCGTCACCGCGGTCGGCCCGCTGCTCGCGCCGCTGATCGGCGGACAGATCCTG
CGCTTTGCCGGCTGGCGCGGCGTGTTCGTCGTGCTCGCCGCGTTCGGCGCGCTATGCGCGGCGACCGCGTATCTGCGCGT
GCCCGAAACCTGGCCGAAGGAGCGCCGCAAGAGCGCGGCCGTGCTCGCGTCGTTCGCGTCGTACGGGCGCATCCTGTCCG
ACCCCGTCGCGTGGGGCCACATGCTGTGCGGCGGGATGGCGTTCGCGTCGATGTTCTCGTACATCACCGCGACGCCGTTC
GTGTACATCGAGTATTTCCACGTGTCGCCGCAGCACTACGGCCTGCTGTTCGGGCTCAACGTGGTCGGCATCATGATCGG
CAACTTCACGAATACGCGGCTCGTCGGCCGGCTCGGCTCGCTGCGCATCATCTCGGCCGCGTCGCTCGTGAGCTGCGTCG
CGTCGCTCGCGGTCGCGCTCGTCGCGCTGACGGGGTGGGGCGGGCTGTGGTCGATCGTCGTCTGTCTGTTCTTCGTCGTC
GGCGTGGTCGGGATTCTGTCCGCGAACTGCACGACCGACCTGATGCATCGCTATCCGCACAACGCGGGCGCGTCGGCGGC
CGTGTTCGGCGCGATGCAGCTCGCGCTCGGCGCGCTCGCGAGCGTCGCCATCGGCGTGCTCGCGGACGGCACGCCGTTCG
CGATGGGCGTGACGATCGGTGTGACCGGCGTGCTCTGTTTCGTCGGACGTTATCTCGTGCTGCGCTGGCACGGGCGGCCG
GTCAAAGCGGCCGCCTGA

Upstream 100 bases:

>100_bases
TTGCGCGTACAATCATGCTCCTGAGCGCGCCGCATACGGCGCGACTTCGTCCAGACCGCCACGCGCGCCGCGAAGCGCGC
GGGCTTCGACCCGGAATCCC

Downstream 100 bases:

>100_bases
GCGCGTCGCCGCATGCAAAACGCCACCGCGCGCGCTTGCGCCGGTGGCGTTTTGCTTTGGGCACGCGCAGGCGTGCCCGC
GAGCGCGCTGCGTCAGTCGC

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSHVVRRRPDARLILLLGALAACGPIATDMYLPSLPSIAQGFSVNPGAAQRTLTSFMAGFSIGMLLYGPLSDTWGRRPVL
LGGIALFTLASVGCFVSTSIDMLIVVRFLQALGAGAASVLARAIARDAHEPTDAAKVLSMVAIVTAVGPLLAPLIGGQIL
RFAGWRGVFVVLAAFGALCAATAYLRVPETWPKERRKSAAVLASFASYGRILSDPVAWGHMLCGGMAFASMFSYITATPF
VYIEYFHVSPQHYGLLFGLNVVGIMIGNFTNTRLVGRLGSLRIISAASLVSCVASLAVALVALTGWGGLWSIVVCLFFVV
GVVGILSANCTTDLMHRYPHNAGASAAVFGAMQLALGALASVAIGVLADGTPFAMGVTIGVTGVLCFVGRYLVLRWHGRP
VKAAA

Sequences:

>Translated_405_residues
MSHVVRRRPDARLILLLGALAACGPIATDMYLPSLPSIAQGFSVNPGAAQRTLTSFMAGFSIGMLLYGPLSDTWGRRPVL
LGGIALFTLASVGCFVSTSIDMLIVVRFLQALGAGAASVLARAIARDAHEPTDAAKVLSMVAIVTAVGPLLAPLIGGQIL
RFAGWRGVFVVLAAFGALCAATAYLRVPETWPKERRKSAAVLASFASYGRILSDPVAWGHMLCGGMAFASMFSYITATPF
VYIEYFHVSPQHYGLLFGLNVVGIMIGNFTNTRLVGRLGSLRIISAASLVSCVASLAVALVALTGWGGLWSIVVCLFFVV
GVVGILSANCTTDLMHRYPHNAGASAAVFGAMQLALGALASVAIGVLADGTPFAMGVTIGVTGVLCFVGRYLVLRWHGRP
VKAAA
>Mature_404_residues
SHVVRRRPDARLILLLGALAACGPIATDMYLPSLPSIAQGFSVNPGAAQRTLTSFMAGFSIGMLLYGPLSDTWGRRPVLL
GGIALFTLASVGCFVSTSIDMLIVVRFLQALGAGAASVLARAIARDAHEPTDAAKVLSMVAIVTAVGPLLAPLIGGQILR
FAGWRGVFVVLAAFGALCAATAYLRVPETWPKERRKSAAVLASFASYGRILSDPVAWGHMLCGGMAFASMFSYITATPFV
YIEYFHVSPQHYGLLFGLNVVGIMIGNFTNTRLVGRLGSLRIISAASLVSCVASLAVALVALTGWGGLWSIVVCLFFVVG
VVGILSANCTTDLMHRYPHNAGASAAVFGAMQLALGALASVAIGVLADGTPFAMGVTIGVTGVLCFVGRYLVLRWHGRPV
KAAA

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=397, Percent_Identity=33.7531486146096, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI48994889, Length=396, Percent_Identity=27.5252525252525, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI87082312, Length=389, Percent_Identity=28.0205655526992, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1790146, Length=202, Percent_Identity=33.6633663366337, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1787065, Length=257, Percent_Identity=27.6264591439689, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1790794, Length=223, Percent_Identity=25.1121076233184, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1790195, Length=163, Percent_Identity=29.4478527607362, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787880, Length=233, Percent_Identity=27.8969957081545, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319657, Length=147, Percent_Identity=27.891156462585, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324264, Length=178, Percent_Identity=23.5955056179775, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42290; Mature: 42158

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHVVRRRPDARLILLLGALAACGPIATDMYLPSLPSIAQGFSVNPGAAQRTLTSFMAGF
CCCCHHCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHH
SIGMLLYGPLSDTWGRRPVLLGGIALFTLASVGCFVSTSIDMLIVVRFLQALGAGAASVL
HHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ARAIARDAHEPTDAAKVLSMVAIVTAVGPLLAPLIGGQILRFAGWRGVFVVLAAFGALCA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ATAYLRVPETWPKERRKSAAVLASFASYGRILSDPVAWGHMLCGGMAFASMFSYITATPF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCE
VYIEYFHVSPQHYGLLFGLNVVGIMIGNFTNTRLVGRLGSLRIISAASLVSCVASLAVAL
EEEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VALTGWGGLWSIVVCLFFVVGVVGILSANCTTDLMHRYPHNAGASAAVFGAMQLALGALA
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
SVAIGVLADGTPFAMGVTIGVTGVLCFVGRYLVLRWHGRPVKAAA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCC
>Mature Secondary Structure 
SHVVRRRPDARLILLLGALAACGPIATDMYLPSLPSIAQGFSVNPGAAQRTLTSFMAGF
CCCHHCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHH
SIGMLLYGPLSDTWGRRPVLLGGIALFTLASVGCFVSTSIDMLIVVRFLQALGAGAASVL
HHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ARAIARDAHEPTDAAKVLSMVAIVTAVGPLLAPLIGGQILRFAGWRGVFVVLAAFGALCA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ATAYLRVPETWPKERRKSAAVLASFASYGRILSDPVAWGHMLCGGMAFASMFSYITATPF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCE
VYIEYFHVSPQHYGLLFGLNVVGIMIGNFTNTRLVGRLGSLRIISAASLVSCVASLAVAL
EEEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VALTGWGGLWSIVVCLFFVVGVVGILSANCTTDLMHRYPHNAGASAAVFGAMQLALGALA
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
SVAIGVLADGTPFAMGVTIGVTGVLCFVGRYLVLRWHGRPVKAAA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]