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The map label for this gene is mltB [H]

Identifier: 161525418

GI number: 161525418

Start: 2461482

End: 2462816

Strand: Direct

Name: mltB [H]

Synonym: Bmul_2248

Alternate gene names: 161525418

Gene position: 2461482-2462816 (Clockwise)

Preceding gene: 161525416

Following gene: 161525435

Centisome position: 71.38

GC content: 66.22

Gene sequence:

>1335_bases
ATGATGTCCATCGTCTGCCGCGAATTCGCCGCTAACTTACCACATCGCGCGCCCGCTTCGCGTCGGCGCGCGGGATGCGT
CGGGTATACTGCGCCGAACACAATCGCCCTGTCCGAGCGCTTCGACATGAATTTCAGCAAGCCTGCCGCCCTTCTCTCCG
CGCTGTTCCGCGCTCGCCTTCCGCTCGTCGTCGCTGCCGTCGCCGCATTGTGCGCGGCGCCCGCCGGCGCACAGACGGCG
CCCGCGAAACCCGGCATGCGCGTCGCGCAGGCGCAGCCGCAACAGCCGGGCCAGACCTTCGAAGAAGAGATCGTTCCGCA
GCGCTACGCGAACAATCCGAAGATCGATGCGTTTATCGACGACATGGCGAGCCGCAACGGTTTCGACGCCGCGAGCCTGC
ATGCGCTGTTCTCGCGCGTCAGCTACTCGGCGACGGCCGTCAAGCTCGTGCGGCCCGCACCGACGCCGCCCGTGAAGAAC
TGGCGCGTCTATCGCTCGCGCTTCATCGAGCCGATCCGCATCAACGCGGGCGTGAAGTTCTGGAAGGCGAACCAGGCGAC
GTTGCAGCGCGCGTCGCAGGAGTTCGGCGTGCCGCCGGAAGTAATCGTCGGCATCATCGGCGTCGAAACGATCTACGGCC
GCTACATGGGCAACTTCCGCGTGCTCGATGCGCTGACGACGCTCGCGTTCGACTATCCGGACACGTCGAACCGCGACAGC
CGTCAGGCGACGTTCCGCAAGAACCTCGAGGATTTCCTCGTGTGGACGCGCGACAGCCAGCTCGACCCGACGACCGTGCT
CGGCTCGTACACGGGCGCGATCGGCATTCCGCAGTTCCTGCCGAGCAGCATCCGCGACTATGCGGTCGACTACGACGGCA
CCGGGCGCGTCGATCTGCGCAGCAGCCCGGCCGACGCGATCGGCAGCGTTGCGAACTATCTGAAGCAGCACGGCTGGGAA
ACCGACCGTCCCGTGGTGTGGCAGATCACGCCCGATACGGGCAGCCTCGGCATCGCGCAGGCGGCCGCCGACGGTCAGCC
CGAACCGCACTGGGCGCTGTCGCAACTGCTGCGCGCCGGCATGACGCTGAACGAGCCGACGGTCAACATCACGACGGAAG
CCGGCACGCCCGTTACGGTCGTCGACCTGCCCTCGCCGGGGAGTGCGACCGAATACATGCTCGGCCTCAAGAACTTCTAC
GTGCTGACGCGTTACAACCGCAGCTTCTTCTATGCGCTCGCCGTGTATCAGCTCGGCGAAGCGGTGAAGGCGCAGATGGA
AGCGAACGGTGCACTGCCGCCGAGCGATGCGGACTCGCAGTCGACGTCGCAGTAA

Upstream 100 bases:

>100_bases
CCACTGCCCCATCTCGTGCAGCATGCAGTCGGGGTGCGTATAGAAGGCGGTTGCCATAACGTGTCATCTGCAGCGCTGGA
TGCACCGCAGGTCTCCTTCA

Downstream 100 bases:

>100_bases
TCCCGCCCGCCATCAACAAAAAACGCCGCTGCATGCGGCGTTTTTTGTTGTTGAAGCGCGGCCCGTGCGAGCCGCGCAGC
GTCACGCCGGAAACACGCCG

Product: lytic murein transglycosylase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MMSIVCREFAANLPHRAPASRRRAGCVGYTAPNTIALSERFDMNFSKPAALLSALFRARLPLVVAAVAALCAAPAGAQTA
PAKPGMRVAQAQPQQPGQTFEEEIVPQRYANNPKIDAFIDDMASRNGFDAASLHALFSRVSYSATAVKLVRPAPTPPVKN
WRVYRSRFIEPIRINAGVKFWKANQATLQRASQEFGVPPEVIVGIIGVETIYGRYMGNFRVLDALTTLAFDYPDTSNRDS
RQATFRKNLEDFLVWTRDSQLDPTTVLGSYTGAIGIPQFLPSSIRDYAVDYDGTGRVDLRSSPADAIGSVANYLKQHGWE
TDRPVVWQITPDTGSLGIAQAAADGQPEPHWALSQLLRAGMTLNEPTVNITTEAGTPVTVVDLPSPGSATEYMLGLKNFY
VLTRYNRSFFYALAVYQLGEAVKAQMEANGALPPSDADSQSTSQ

Sequences:

>Translated_444_residues
MMSIVCREFAANLPHRAPASRRRAGCVGYTAPNTIALSERFDMNFSKPAALLSALFRARLPLVVAAVAALCAAPAGAQTA
PAKPGMRVAQAQPQQPGQTFEEEIVPQRYANNPKIDAFIDDMASRNGFDAASLHALFSRVSYSATAVKLVRPAPTPPVKN
WRVYRSRFIEPIRINAGVKFWKANQATLQRASQEFGVPPEVIVGIIGVETIYGRYMGNFRVLDALTTLAFDYPDTSNRDS
RQATFRKNLEDFLVWTRDSQLDPTTVLGSYTGAIGIPQFLPSSIRDYAVDYDGTGRVDLRSSPADAIGSVANYLKQHGWE
TDRPVVWQITPDTGSLGIAQAAADGQPEPHWALSQLLRAGMTLNEPTVNITTEAGTPVTVVDLPSPGSATEYMLGLKNFY
VLTRYNRSFFYALAVYQLGEAVKAQMEANGALPPSDADSQSTSQ
>Mature_444_residues
MMSIVCREFAANLPHRAPASRRRAGCVGYTAPNTIALSERFDMNFSKPAALLSALFRARLPLVVAAVAALCAAPAGAQTA
PAKPGMRVAQAQPQQPGQTFEEEIVPQRYANNPKIDAFIDDMASRNGFDAASLHALFSRVSYSATAVKLVRPAPTPPVKN
WRVYRSRFIEPIRINAGVKFWKANQATLQRASQEFGVPPEVIVGIIGVETIYGRYMGNFRVLDALTTLAFDYPDTSNRDS
RQATFRKNLEDFLVWTRDSQLDPTTVLGSYTGAIGIPQFLPSSIRDYAVDYDGTGRVDLRSSPADAIGSVANYLKQHGWE
TDRPVVWQITPDTGSLGIAQAAADGQPEPHWALSQLLRAGMTLNEPTVNITTEAGTPVTVVDLPSPGSATEYMLGLKNFY
VLTRYNRSFFYALAVYQLGEAVKAQMEANGALPPSDADSQSTSQ

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=326, Percent_Identity=42.638036809816, Blast_Score=236, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 48355; Mature: 48355

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSIVCREFAANLPHRAPASRRRAGCVGYTAPNTIALSERFDMNFSKPAALLSALFRARL
CCHHHHHHHHHCCCCCCCCHHCCCCEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHH
PLVVAAVAALCAAPAGAQTAPAKPGMRVAQAQPQQPGQTFEEEIVPQRYANNPKIDAFID
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHCHHHHCCCCCHHHHHH
DMASRNGFDAASLHALFSRVSYSATAVKLVRPAPTPPVKNWRVYRSRFIEPIRINAGVKF
HHHCCCCCCHHHHHHHHHHHHCCHHEEEEECCCCCCCCCHHHHHHHHCCCCEEECCCEEE
WKANQATLQRASQEFGVPPEVIVGIIGVETIYGRYMGNFRVLDALTTLAFDYPDTSNRDS
EECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCH
RQATFRKNLEDFLVWTRDSQLDPTTVLGSYTGAIGIPQFLPSSIRDYAVDYDGTGRVDLR
HHHHHHHCHHHHEEECCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHEECCCCCCCEEEC
SSPADAIGSVANYLKQHGWETDRPVVWQITPDTGSLGIAQAAADGQPEPHWALSQLLRAG
CCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHCCCCCCCCCHHHHHHHHCC
MTLNEPTVNITTEAGTPVTVVDLPSPGSATEYMLGLKNFYVLTRYNRSFFYALAVYQLGE
CCCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHH
AVKAQMEANGALPPSDADSQSTSQ
HHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMSIVCREFAANLPHRAPASRRRAGCVGYTAPNTIALSERFDMNFSKPAALLSALFRARL
CCHHHHHHHHHCCCCCCCCHHCCCCEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHH
PLVVAAVAALCAAPAGAQTAPAKPGMRVAQAQPQQPGQTFEEEIVPQRYANNPKIDAFID
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHCHHHHCCCCCHHHHHH
DMASRNGFDAASLHALFSRVSYSATAVKLVRPAPTPPVKNWRVYRSRFIEPIRINAGVKF
HHHCCCCCCHHHHHHHHHHHHCCHHEEEEECCCCCCCCCHHHHHHHHCCCCEEECCCEEE
WKANQATLQRASQEFGVPPEVIVGIIGVETIYGRYMGNFRVLDALTTLAFDYPDTSNRDS
EECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCH
RQATFRKNLEDFLVWTRDSQLDPTTVLGSYTGAIGIPQFLPSSIRDYAVDYDGTGRVDLR
HHHHHHHCHHHHEEECCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHEECCCCCCCEEEC
SSPADAIGSVANYLKQHGWETDRPVVWQITPDTGSLGIAQAAADGQPEPHWALSQLLRAG
CCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHCCCCCCCCCHHHHHHHHCC
MTLNEPTVNITTEAGTPVTVVDLPSPGSATEYMLGLKNFYVLTRYNRSFFYALAVYQLGE
CCCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHH
AVKAQMEANGALPPSDADSQSTSQ
HHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]