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The map label for this gene is rlmB [H]

Identifier: 161524858

GI number: 161524858

Start: 1853668

End: 1854411

Strand: Reverse

Name: rlmB [H]

Synonym: Bmul_1685

Alternate gene names: 161524858

Gene position: 1854411-1853668 (Counterclockwise)

Preceding gene: 161524859

Following gene: 161524857

Centisome position: 53.77

GC content: 68.82

Gene sequence:

>744_bases
ATGTCACGTCTGAAGGTTCTTTACGGTTTTCATGCGGTGACCGCACGCTTGCGGCACGATGCAGCGACGGTGGTGGAGGT
GCTGTACGACCAGACGCGTCGCGATCGCCGCATGCAGGACTTCCTGCACGCCGCGAAGGAAGCGGGCGTGCGGCTGATCG
CAGCCGACGAGACGCGCCTGTGGGGGCTCGCGCATACCGAGCGTCACCAGGGCGTCGTCGCGCGCGTCGAAGACCTCCCG
CTCGCGCAAAACCTGTCCGAGCTGCTCGACGGCATTCAAGGGCCGGCGCTGTTGCTCGTGCTCGACGGCGTCACCGATCC
GCACAACCTCGGCGCGTGCCTGCGCGTCGCCGATGCGGCCGGCGCGCACGCGGTGATCGCGCCGCGCGACCGCGCGGTCG
GGCTGAACGCAACGGCGGCGAAGGTCGCGAGCGGCGCCGCCGACACCGTGCCGTACATCACGGTCACGAACCTGGCGCGT
GCGCTGCGCGAGCTGAAGGAAGCCGGCGTGTGGATCATCGGCACGTCGGACGAAGCGTCGGCGACGCTGTACGACACGAA
GCTCGACGGCCCGGTTGCGATCGTGATGGGCGCCGAAGGCGAAGGCATGCGCCGCCTCACGCGCGACACGTGCGACGAAG
TGATGAGCATTCCGATGGCGGGCAGCGTCGAAAGCCTGAACGTGTCGGTGGCGAGCGGCGTATGCCTGTACGAAGCGGTT
CGGCAGCGGCGCGCGAAAGGCTGA

Upstream 100 bases:

>100_bases
CGTCGCCGGTCATGTGCCGGCCACGCGGCGCTTTCCTTTTCCATGGATCGCGGCTGCGCGCGTCGTGCGGCCGCACGTTT
GATCGAAGGTTGTTCCAGTC

Downstream 100 bases:

>100_bases
GGCAGCGGCCGCGCCGCGAGCGCGCAGCACGATGCGCATCGCGCGCCCGCAAGCGCAGCGGCGCAAGTTTTCTTCGGGTT
CTGGATCGATGACATCGTTT

Product: RNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSRLKVLYGFHAVTARLRHDAATVVEVLYDQTRRDRRMQDFLHAAKEAGVRLIAADETRLWGLAHTERHQGVVARVEDLP
LAQNLSELLDGIQGPALLLVLDGVTDPHNLGACLRVADAAGAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLAR
ALRELKEAGVWIIGTSDEASATLYDTKLDGPVAIVMGAEGEGMRRLTRDTCDEVMSIPMAGSVESLNVSVASGVCLYEAV
RQRRAKG

Sequences:

>Translated_247_residues
MSRLKVLYGFHAVTARLRHDAATVVEVLYDQTRRDRRMQDFLHAAKEAGVRLIAADETRLWGLAHTERHQGVVARVEDLP
LAQNLSELLDGIQGPALLLVLDGVTDPHNLGACLRVADAAGAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLAR
ALRELKEAGVWIIGTSDEASATLYDTKLDGPVAIVMGAEGEGMRRLTRDTCDEVMSIPMAGSVESLNVSVASGVCLYEAV
RQRRAKG
>Mature_246_residues
SRLKVLYGFHAVTARLRHDAATVVEVLYDQTRRDRRMQDFLHAAKEAGVRLIAADETRLWGLAHTERHQGVVARVEDLPL
AQNLSELLDGIQGPALLLVLDGVTDPHNLGACLRVADAAGAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLARA
LRELKEAGVWIIGTSDEASATLYDTKLDGPVAIVMGAEGEGMRRLTRDTCDEVMSIPMAGSVESLNVSVASGVCLYEAVR
QRRAKG

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=264, Percent_Identity=26.1363636363636, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1790623, Length=241, Percent_Identity=55.1867219917012, Blast_Score=256, Evalue=1e-69,
Organism=Escherichia coli, GI1788935, Length=244, Percent_Identity=30.327868852459, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790083, Length=157, Percent_Identity=37.5796178343949, Blast_Score=82, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17555908, Length=256, Percent_Identity=26.171875, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324775, Length=186, Percent_Identity=30.1075268817204, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26427; Mature: 26295

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLKVLYGFHAVTARLRHDAATVVEVLYDQTRRDRRMQDFLHAAKEAGVRLIAADETRL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEE
WGLAHTERHQGVVARVEDLPLAQNLSELLDGIQGPALLLVLDGVTDPHNLGACLRVADAA
EECCHHHHHCCCEEHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCC
GAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLARALRELKEAGVWIIGTSDEAS
CCEEEECCCCCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCCCC
ATLYDTKLDGPVAIVMGAEGEGMRRLTRDTCDEVMSIPMAGSVESLNVSVASGVCLYEAV
EEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHCEEHHHCCHHHHHHH
RQRRAKG
HHHHCCC
>Mature Secondary Structure 
SRLKVLYGFHAVTARLRHDAATVVEVLYDQTRRDRRMQDFLHAAKEAGVRLIAADETRL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEE
WGLAHTERHQGVVARVEDLPLAQNLSELLDGIQGPALLLVLDGVTDPHNLGACLRVADAA
EECCHHHHHCCCEEHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCC
GAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLARALRELKEAGVWIIGTSDEAS
CCEEEECCCCCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCCCC
ATLYDTKLDGPVAIVMGAEGEGMRRLTRDTCDEVMSIPMAGSVESLNVSVASGVCLYEAV
EEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHCEEHHHCCHHHHHHH
RQRRAKG
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]