Click here to switch to the map view.

The map label for this gene is amiD [H]

Identifier: 161524857

GI number: 161524857

Start: 1852704

End: 1853579

Strand: Reverse

Name: amiD [H]

Synonym: Bmul_1684

Alternate gene names: 161524857

Gene position: 1853579-1852704 (Counterclockwise)

Preceding gene: 161524858

Following gene: 161524856

Centisome position: 53.75

GC content: 68.95

Gene sequence:

>876_bases
ATGACATCGTTTCGCGTCGGCGCGTCGTGCGCCGCCTTGTGCCTGCTTGCCGCGTGCGCGTCGCCGTCGCTGGTCGAACG
CGGCAGCTACTACGCCGATACGAGCCTGCACGCGCGCGGCGCCGACTCGCGGATTCGCTTTCTCGTGATGCACTACACCG
AAAGCGACGAGGCGAAATCGTTGCGCACGCTGACGGGCGATACGGTCAGCGTGCACTACGTGGTGCCGGCTCATCCACGC
AGCGAGCGCGGGCAGCCGGTCGTCTATCAGCTCGTGCCCGAAGCACAGCGCGCGTGGCATGCGGGCGTCAGCGCATGGCA
GCAGACGACCGAACTGAACGCGGCATCGATCGGCATCGAGAACGTGAATCGCGGGCCGCTCGATCCGCAGCAGCGCACGT
GGCAGCCGTATCCGCCGGAACAGGTCGATGCGCTGATCCGGCTGTCGAAGGACATCGTCGCGCGTTACGGGATCGCACCG
ACGCGCGTGGTCGGACACAGCGACATCGCACCGCAACGCAAGATCGATCCGGGCCCGCTGTTTCCGTGGCGCGCGCTCGC
ACAAGCCGGCGTCGGCGCGTGGCCCGACGACGCGACGGTCGCCGCGCGGCTCGCGGGCCGCGACCCGCATGCACCCGTCG
ACGTGCGCGAGCTGCAGACGAAGCTCGCGCGCTACGGCTACGACGTGCCGACCGACGGCGTGCTCGACACGCGCACGCGC
CGCGTGTTTTCGGCGTTCCAGATGCACTTTCGTCCGTCCGACTATGCGGGCGATGCCGATGCCGAGACGGACGCGATCGC
GCAGGCGCTGCTCGACAAGTATTTCCCGGGCCAGCCGCCGATGGATGGCGCGCCGTCCGACGTCGATCCGCAGTGA

Upstream 100 bases:

>100_bases
GCGAAAGGCTGAGGCAGCGGCCGCGCCGCGAGCGCGCAGCACGATGCGCATCGCGCGCCCGCAAGCGCAGCGGCGCAAGT
TTTCTTCGGGTTCTGGATCG

Downstream 100 bases:

>100_bases
GCGACCAGCGCGCCGAAAACGGGCGGCCGCTCGCGCGAGTCCGGTAAACTGGCGGTTTTTCCGCAATCCTGCAGCATGAC
CCATCCCATGACTCAAGACG

Product: N-acetylmuramyl-L-alanine amidase, negative regulator of AmpC, AmpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTSFRVGASCAALCLLAACASPSLVERGSYYADTSLHARGADSRIRFLVMHYTESDEAKSLRTLTGDTVSVHYVVPAHPR
SERGQPVVYQLVPEAQRAWHAGVSAWQQTTELNAASIGIENVNRGPLDPQQRTWQPYPPEQVDALIRLSKDIVARYGIAP
TRVVGHSDIAPQRKIDPGPLFPWRALAQAGVGAWPDDATVAARLAGRDPHAPVDVRELQTKLARYGYDVPTDGVLDTRTR
RVFSAFQMHFRPSDYAGDADAETDAIAQALLDKYFPGQPPMDGAPSDVDPQ

Sequences:

>Translated_291_residues
MTSFRVGASCAALCLLAACASPSLVERGSYYADTSLHARGADSRIRFLVMHYTESDEAKSLRTLTGDTVSVHYVVPAHPR
SERGQPVVYQLVPEAQRAWHAGVSAWQQTTELNAASIGIENVNRGPLDPQQRTWQPYPPEQVDALIRLSKDIVARYGIAP
TRVVGHSDIAPQRKIDPGPLFPWRALAQAGVGAWPDDATVAARLAGRDPHAPVDVRELQTKLARYGYDVPTDGVLDTRTR
RVFSAFQMHFRPSDYAGDADAETDAIAQALLDKYFPGQPPMDGAPSDVDPQ
>Mature_290_residues
TSFRVGASCAALCLLAACASPSLVERGSYYADTSLHARGADSRIRFLVMHYTESDEAKSLRTLTGDTVSVHYVVPAHPRS
ERGQPVVYQLVPEAQRAWHAGVSAWQQTTELNAASIGIENVNRGPLDPQQRTWQPYPPEQVDALIRLSKDIVARYGIAPT
RVVGHSDIAPQRKIDPGPLFPWRALAQAGVGAWPDDATVAARLAGRDPHAPVDVRELQTKLARYGYDVPTDGVLDTRTRR
VFSAFQMHFRPSDYAGDADAETDAIAQALLDKYFPGQPPMDGAPSDVDPQ

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=259, Percent_Identity=55.5984555984556, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1786300, Length=112, Percent_Identity=38.3928571428571, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 31784; Mature: 31653

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSFRVGASCAALCLLAACASPSLVERGSYYADTSLHARGADSRIRFLVMHYTESDEAKS
CCCCCCCHHHHHHHHHHHHCCCHHHHCCCCEECCCCCCCCCCCEEEEEEEEECCCCHHHH
LRTLTGDTVSVHYVVPAHPRSERGQPVVYQLVPEAQRAWHAGVSAWQQTTELNAASIGIE
HHHCCCCEEEEEEEECCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCHHHCCCC
NVNRGPLDPQQRTWQPYPPEQVDALIRLSKDIVARYGIAPTRVVGHSDIAPQRKIDPGPL
CCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCC
FPWRALAQAGVGAWPDDATVAARLAGRDPHAPVDVRELQTKLARYGYDVPTDGVLDTRTR
CHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHH
RVFSAFQMHFRPSDYAGDADAETDAIAQALLDKYFPGQPPMDGAPSDVDPQ
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSFRVGASCAALCLLAACASPSLVERGSYYADTSLHARGADSRIRFLVMHYTESDEAKS
CCCCCCHHHHHHHHHHHHCCCHHHHCCCCEECCCCCCCCCCCEEEEEEEEECCCCHHHH
LRTLTGDTVSVHYVVPAHPRSERGQPVVYQLVPEAQRAWHAGVSAWQQTTELNAASIGIE
HHHCCCCEEEEEEEECCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCHHHCCCC
NVNRGPLDPQQRTWQPYPPEQVDALIRLSKDIVARYGIAPTRVVGHSDIAPQRKIDPGPL
CCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCC
FPWRALAQAGVGAWPDDATVAARLAGRDPHAPVDVRELQTKLARYGYDVPTDGVLDTRTR
CHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHH
RVFSAFQMHFRPSDYAGDADAETDAIAQALLDKYFPGQPPMDGAPSDVDPQ
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]